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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFD687
Library SF
(Link to library)
Clone ID SFD687
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFD687P
(Link to Original site)
Representative DNA sequence 
>SFD687 (SFD687Q) /CSM/SF/SFD6-D/SFD687Q.Seq.d/
ACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGT
GATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAA
GTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTC
TTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATT
ACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATT
TCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGT
TTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACA
TTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGT
GATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAA
TTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTTTAAGA
ACTGCTTGTGAACGTGCCAAXXXXXXXXXXCAGGTGTATTAATTCAAGTTTATGAAGGTG
AACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCAC
CAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCT
TAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATG
ATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCA
AACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATG
CATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAG
ATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAA
CCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAA
TCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTA
TGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATT
TTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATC
AATAAAGCTTCTCATATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
TVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVL
LKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYG
LEKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDE
FKRKHKKDIMGNQRAVRRLRTACERA---

---GVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDK
STGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSI
KDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQE
GGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sfsy
Translated Amino Acid sequence (All Frames) 
Frame A:
TVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVL
LKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYG
LEKKGSGEKNILIFDLGGGTFDVSLLTIEDGVFEVKATAGDTHLGGEDFDNRLVSHFVDE
FKRKHKKDIMGNQRAVRRLRTACERA---

---qvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*myqlki
nqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsllkiq
lkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*vnsik
kevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vskinkashi

Frame B:
qylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifklnskvklkfshqkkslqwys
*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*mfnvllmnqllllllmv
*krkvlvkrif*fsi*vvvhlmflslplkmvylklkpplvili*vvkiliivsfltllmn
lnvnikkilwvinvlfvv*ellvnvp---

---rcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkcis*r*
inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc*kfn
*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*tlsr
rryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvksiklli*

Frame C:
si*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iqr*n*sfltrrnlfngtl
knernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkikcstyy**tncccyclwf
rkerfw*keyfnfrfrwwyi*cfspyh*rwci*s*shrw*ysfrw*rf**ssrfslc**i
*t*t*kryyg*stccssfkncl*tcq---

---GVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVSAEDK
STGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTVKNSI
KDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSKLYQE
GGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq*sfsy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFD687 (SFD687Q) /CSM/SF/SFD6-D/SFD687Q.Seq.d/                       2595   0.0
VFN458 (VFN458Q) /CSM/VF/VFN4-C/VFN458Q.Seq.d/                       1405   0.0
VFG861 (VFG861Q) /CSM/VF/VFG8-C/VFG861Q.Seq.d/                       1405   0.0
SFK617 (SFK617Q) /CSM/SF/SFK6-A/SFK617Q.Seq.d/                       1405   0.0
SFI687 (SFI687Q) /CSM/SF/SFI6-D/SFI687Q.Seq.d/                       1405   0.0
SFG515 (SFG515Q) /CSM/SF/SFG5-A/SFG515Q.Seq.d/                       1405   0.0
SFB319 (SFB319Q) /CSM/SF/SFB3-A/SFB319Q.Seq.d/                       1405   0.0
CHR221 (CHR221Q) /CSM/CH/CHR2-A/CHR221Q.Seq.d/                       1405   0.0
CHI410 (CHI410Q) /CSM/CH/CHI4-A/CHI410Q.Seq.d/                       1405   0.0
CHH144 (CHH144Q) /CSM/CH/CHH1-B/CHH144Q.Seq.d/                       1405   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1404   0.0    1
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1396   0.0    1
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   293   e-180  9
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   293   e-172  13
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 121   e-127  11
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          121   e-127  11
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   121   e-127  11
AF278536|AF278536.1 Parastrongyloides trichosuri heat shock 70 protein gene, complete cds.   133   e-116  11
AJ310880|AJ310880.1 Cryptosporidium baileyi partial hsp70 gene for heat shock protein  70.   100   e-110  9
AF221539|AF221539.1 Cryptosporidium baileyi heat shock protein 70 (HSP70) gene, partial cds.   100   e-110  9
dna update 2003.12.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   411   e-113
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   409   e-112
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   382   e-104
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   378   e-103
AY582833_1(AY582833|pid:none) Chytriomyces confervae strain CBS ...   358   2e-97
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           358   2e-97
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   353   8e-96
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   353   8e-96
EU679414_1(EU679414|pid:none) Tetranychus cinnabarinus heat shoc...   353   1e-95
EU679412_1(EU679412|pid:none) Tetranychus cinnabarinus heat shoc...   350   8e-95
protein update 2009. 5.23
PSORT 

 psg: 0.42  gvh: 0.25  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.03
 nuc: 0.14  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for SFD687 is nuc
5' end seq. ID SFD687F
5' end seq. 
>SFD687F.Seq
ACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGT
GATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAA
GTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTC
TTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATT
ACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATT
TCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGT
TTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACA
TTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGT
GATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAA
TTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTTTAAGA
ACTGCTTGTGAACGTGCCAA----------
Length of 5' end seq. 620
3' end seq. ID SFD687Z
3' end seq. 
>SFD687Z.Seq
----------CAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGAT
AATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCA
CAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGAT
AAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAA
GAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAA
AAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCA
ATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCT
GAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATAT
GAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAA
GAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAG
AGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCT
ATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATATAA
Length of 3' end seq. 709
Connected seq. ID SFD687P
Connected seq. 
>SFD687P.Seq
ACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGT
GATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAA
GTTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTC
TTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATT
ACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATT
TCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGT
TTAGAAAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACA
TTTGATGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGAAGTTAAAGCCACCGCTGGT
GATACTCATTTAGGTGGTGAAGATTTTGATAATCGTCTCGTTTCTCACTTTGTTGATGAA
TTTAAACGTAAACATAAAAAAGATATTATGGGTAATCAACGTGCTGTTCGTCGTTTAAGA
ACTGCTTGTGAACGTGCCAA----------CAGGTGTATTAATTCAAGTTTATGAAGGTG
AACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCAC
CAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCT
TAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATG
ATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCA
AACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATG
CATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAG
ATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAA
CCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAA
TCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTA
TGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATT
TTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATC
AATAAAGCTTCTCATATAA
Length of connected seq. 1329
Full length Seq ID -
Full length Seq. -
Length of full length seq. -