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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFI123
Library AF
(Link to library)
Clone ID AFI123
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFI123P
(Link to Original site)
Representative DNA sequence 
>AFI123 (AFI123Q) /CSM/AF/AFI1-A/AFI123Q.Seq.d/
CTGTTGGCCTACTGGTAACACACCAAATATATATTTATATTTATATATTTATATATTTTT
TTTTATTTTATTTTCACACCCTATTCTATATAAATTAATTTTTTTTTTTTTANCACCAAA
TTAAAAAAAAAAXXXXXXXXXXTATATGCTTAATAAACCAATTGAAAATATTGTTTTCGA
TGAAGAAAAGAAAATTATTAAAGTTACATCTGAAGGTGAAACTGTAACATGTGATAAAAT
TGTTTGTGATCCATCATATTTCCCAAATAAAGTTCAAAANAAAGGTAAAATTATTCGTTC
AATTTGTATTTTGAATGGTCCAATTCCATCTGCTGATAANAAATTTGAATCACTCCAAAT
CATTATCCCNCAAAAACAAGTTAAACGTCAATCAGATATGTACGTTGGTGTTATGTCATC
CGCTCATTGTGTCTGTCCAAAGGGTAAATACATTGCCATCGTTTCAGCCNCTGTTGAAAC
CAATGAACCAGAGAAAGAATTAGCNCCAGCTTACGCTTTATTAGGTCCAATTCAANAGAA
ATTTGTCTATATTTCTGATTTCATTGAACCAATTGCTGATGGNACTGCCGACAATGTTTT
CATCTCAAAATCATATGATGCTACCTCNCNCTTTGAAACCACNTGTGAAGATGTCATGGA
TATGGACAAGAGAATCNCTGGTAAAGATTTAGTTCNCNCTGNTCATCCAAANATTCTTNA
ACAACAAGAAGAAG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
llayw*htkyififiylyiffyfiftpysi*INFFFFXTKLKKK---

---YMLNKPIENIVFDEEKKIIKVTSEGETVTCDKIVCDPSYFPNKVQXKGKIIRSICIL
NGPIPSADXKFESLQIIIPQKQVKRQSDMYVGVMSSAHCVCPKGKYIAIVSAXVETNEPE
KELAPAYALLGPIQXKFVYISDFIEPIADGTADNVFISKSYDATSXFETTCEDVMDMDKR
IXGKDLVXXXHPXILXQQEE
Translated Amino Acid sequence (All Frames) 
Frame A:
llayw*htkyififiylyiffyfiftpysi*INFFFFXTKLKKK---

---YMLNKPIENIVFDEEKKIIKVTSEGETVTCDKIVCDPSYFPNKVQXKGKIIRSICIL
NGPIPSADXKFESLQIIIPQKQVKRQSDMYVGVMSSAHCVCPKGKYIAIVSAXVETNEPE
KELAPAYALLGPIQXKFVYISDFIEPIADGTADNVFISKSYDATSXFETTCEDVMDMDKR
IXGKDLVXXXHPXILXQQEE

Frame B:
cwptgntpniylylyiyifffilfshpilykliffflxpn*kk---

---iclinqlkilfsmkkrkllklhlkvkl*hviklfvihhisqikfkxkvklfvqfvf*
mvqfhllixnlnhskslsxknklnvnqictlvlchplivsvqrvntlpsfqpllkpmnqr
kn*xqltly*vqfxrnlsiflislnqllmxlptmfssqnhmmlpxxlkpxvkmswiwtre
slvki*fxlxiqxfxnnkkk

Frame C:
vgllvthqiyiyiyifiyfflfyfhtlfyin*ffffxhqikkk---

---ya**tn*kycfr*rkeny*syi*r*ncnm**ncl*siifpk*sskxr*nysfnlyfe
wsnsic**xi*itpnhypxkts*tsiryvrwcyvirslclskg*ihchrfsxc*nq*tre
risxslrfirsnsxeiclyf*fh*tnc*wxcrqcfhlkii*cylxl*nhx*rchgygqen
xw*rfssxxsskxsxttrr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI123 (AFI123Q) /CSM/AF/AFI1-A/AFI123Q.Seq.d/                       1037   0.0
SFJ795 (SFJ795Q) /CSM/SF/SFJ7-D/SFJ795Q.Seq.d/                       1029   0.0
SFJ449 (SFJ449Q) /CSM/SF/SFJ4-C/SFJ449Q.Seq.d/                       1029   0.0
SFI510 (SFI510Q) /CSM/SF/SFI5-A/SFI510Q.Seq.d/                       1029   0.0
SFE141 (SFE141Q) /CSM/SF/SFE1-B/SFE141Q.Seq.d/                       1029   0.0
SFD219 (SFD219Q) /CSM/SF/SFD2-A/SFD219Q.Seq.d/                       1029   0.0
CHD258 (CHD258Q) /CSM/CH/CHD2-C/CHD258Q.Seq.d/                       1029   0.0
CHB577 (CHB577Q) /CSM/CH/CHB5-D/CHB577Q.Seq.d/                       1029   0.0
CFC760 (CFC760Q) /CSM/CF/CFC7-C/CFC760Q.Seq.d/                       1029   0.0
AFN874 (AFN874Q) /CSM/AF/AFN8-D/AFN874Q.Seq.d/                       1029   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI315670|BI315670.1 saf75f12.y1 Gm-c1078 Glycine max cDNA clone GENOME SYSTEMS CLONEINHIBITOR. ;, mRNA sequence.    62   1e-05  1
AI437629|AI437629.1 sa37e09.y1 Gm-c1004 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1004-1505 5' similar to TR:O24653 O24653 GDI2. ;, mRNA sequence.    62   1e-05  1
BU964562|BU964562.1 sas99a12.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-12311 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   1e-05  1
BE800797|BE800797.1 sq98c12.y1 Gm-c1049 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1049-1199 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   1e-05  1
AW397479|AW397479.1 sg79d05.y1 Gm-c1007 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1007-2626 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   1e-05  1
BG790353|BG790353.1 sae68c10.y1 Gm-c1064 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1064-3212 5' similar to TR:O65744 O65744 GDP DISSOCIATION INHIBITOR. ;, mRNA sequence.    62   1e-05  1
AW909710|AW909710.1 ur73b01.y1 NCI_CGAP_Mam3 Mus musculus cDNA clone IMAGE:3155881 5' similar to gb:L05086 Human smg p25A GDP dissociation inhibitor homologue mRNA, partial (HUMAN); gb:U07951 Mus musculus BALB/c GDP-dissociation inhibitor (MOUSE);, mRNA sequence.    34   2e-04  4
BE382073|BE382073.1 601272233F1 NCI_CGAP_Mam1 Mus musculus cDNA clone IMAGE:3601424 5', mRNA sequence.    34   2e-04  4
CN674651|CN674651.1 A0951G10-5 NIA Mouse Embryonic Stem (ES) cell (Lif+, 48 h, high density) cDNA library (Long) Mus musculus cDNA clone NIA:A0951G10 IMAGE:30771729 5', mRNA sequence.    34   3e-04  4
BI149486|BI149486.1 602849057F1 NCI_CGAP_Lu29 Mus musculus cDNA clone IMAGE:5012270 5', mRNA sequence.    34   4e-04  4
dna update 2004. 6.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF676817_1(EF676817|pid:none) Picea sitchensis clone WS02753_I09...   198   1e-49
EF085049_1(EF085049|pid:none) Picea sitchensis clone WS0297_P09 ...   198   1e-49
AF251042_1(AF251042|pid:none) Mus musculus rab GDP dissociation ...   194   2e-48
BT071197_1(BT071197|pid:none) Picea sitchensis clone WS0279_A10 ...   194   3e-48
AF130987_1(AF130987|pid:none) Rattus norvegicus RAB GDP dissocia...   193   5e-48
(O97555) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   193   5e-48
(P50398) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   193   5e-48
X74402_1(X74402|pid:none) R.norvegicus rab GDI alpha mRNA.            193   5e-48
(P21856) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   192   9e-48
(Q8HXX7) RecName: Full=Rab GDP dissociation inhibitor alpha;    ...   192   1e-47
protein update 2009. 4.22
PSORT 

 psg: 0.49  gvh: 0.30  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFI123 is nuc
5' end seq. ID AFI123F
5' end seq. 
>AFI123F.Seq
CTGTTGGCCTACTGGTAACACACCAAATATATATTTATATTTATATATTTATATATTTTT
TTTTATTTTATTTTCACACCCTATTCTATATAAATTAATTTTTTTTTTTTTANCACCAAA
TTAAAAAAAAAA----------
Length of 5' end seq. 132
3' end seq. ID AFI123Z
3' end seq. 
>AFI123Z.Seq
----------TATATGCTTAATAAACCAATTGAAAATATTGTTTTCGATGAAGAAAAGAA
AATTATTAAAGTTACATCTGAAGGTGAAACTGTAACATGTGATAAAATTGTTTGTGATCC
ATCATATTTCCCAAATAAAGTTCAAAANAAAGGTAAAATTATTCGTTCAATTTGTATTTT
GAATGGTCCAATTCCATCTGCTGATAANAAATTTGAATCACTCCAAATCATTATCCCNCA
AAAACAAGTTAAACGTCAATCAGATATGTACGTTGGTGTTATGTCATCCGCTCATTGTGT
CTGTCCAAAGGGTAAATACATTGCCATCGTTTCAGCCNCTGTTGAAACCAATGAACCAGA
GAAAGAATTAGCNCCAGCTTACGCTTTATTAGGTCCAATTCAANAGAAATTTGTCTATAT
TTCTGATTTCATTGAACCAATTGCTGATGGNACTGCCGACAATGTTTTCATCTCAAAATC
ATATGATGCTACCTCNCNCTTTGAAACCACNTGTGAAGATGTCATGGATATGGACAAGAG
AATCNCTGGTAAAGATTTAGTTCNCNCTGNTCATCCAAANATTCTTNAACAACAAGAAGA
AG
Length of 3' end seq. 592
Connected seq. ID AFI123P
Connected seq. 
>AFI123P.Seq
CTGTTGGCCTACTGGTAACACACCAAATATATATTTATATTTATATATTTATATATTTTT
TTTTATTTTATTTTCACACCCTATTCTATATAAATTAATTTTTTTTTTTTTANCACCAAA
TTAAAAAAAAAA----------TATATGCTTAATAAACCAATTGAAAATATTGTTTTCGA
TGAAGAAAAGAAAATTATTAAAGTTACATCTGAAGGTGAAACTGTAACATGTGATAAAAT
TGTTTGTGATCCATCATATTTCCCAAATAAAGTTCAAAANAAAGGTAAAATTATTCGTTC
AATTTGTATTTTGAATGGTCCAATTCCATCTGCTGATAANAAATTTGAATCACTCCAAAT
CATTATCCCNCAAAAACAAGTTAAACGTCAATCAGATATGTACGTTGGTGTTATGTCATC
CGCTCATTGTGTCTGTCCAAAGGGTAAATACATTGCCATCGTTTCAGCCNCTGTTGAAAC
CAATGAACCAGAGAAAGAATTAGCNCCAGCTTACGCTTTATTAGGTCCAATTCAANAGAA
ATTTGTCTATATTTCTGATTTCATTGAACCAATTGCTGATGGNACTGCCGACAATGTTTT
CATCTCAAAATCATATGATGCTACCTCNCNCTTTGAAACCACNTGTGAAGATGTCATGGA
TATGGACAAGAGAATCNCTGGTAAAGATTTAGTTCNCNCTGNTCATCCAAANATTCTTNA
ACAACAAGAAGAAG
Length of connected seq. 724
Full length Seq ID -
Full length Seq. -
Length of full length seq. -