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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFD272
Library SF
(Link to library)
Clone ID SFD272
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFD272P
(Link to Original site)
Representative DNA sequence 
>SFD272 (SFD272Q) /CSM/SF/SFD2-C/SFD272Q.Seq.d/
AAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGATTTANGTACAACA
TATTCATGTGTTGGTGTTNGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGT
AATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTAATTGGNGATGCT
GCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATT
GGTCGNAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTT
ATTCCAAAGGATGGNGATAAGCCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTT
TTCTCACCAGAAGAAATCTNTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCT
TATCTTGGTAAAACCATTAATAATGCTGTAATTACCGNTCCAGCTTATTTCAATGATAGT
CAACGTNAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATT
AATGAACCAACTGCTGCTGCTATTGCTTATGGTTTXXXXXXXXXXTTCCAAGAAATACAA
CAATTCCCATGTAAGAAAACTCAAACCTTCTCTACATACTCTGATAATCANCCAGGTGTA
TTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAA
TTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTT
GATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAA
CAAAAGATTACAATTGCCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATG
GTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACACAAAAAGATCGTGTTGAATCAA
AGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTG
CAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCA
AATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCT
TAGAAGCAGTTGTCAATCCAGATCATGAGTNAACTCNATCAAGAAGGAGGTATGCCACAA
GGTGGTGGTACTGCCAGGTGGTATGTCAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ky*iiky*KMSSIGIDLXTTYSCVGVXQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDA
AKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKV
FSPEEIXSMVLLKMKETAEAYLGKTINNAVITXPAYFNDSQRXATKDAGTISKLNVQRII
NEPTAAAIAYG---

---FQEIQQFPCKKTQTFSTYSDNXPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRG
VPQVEVTFDVDANGILNVSAEDKSTGNKQKITIANDKGRLSKEEIEKMVADAEKFKQQDE
HKKIVLNQRINWKIMHSLLKIQLKMKKLQPKFQIQINQLLNLKLKVYSNG*nqikpqkrm
nmkir*kp*kqlsiqimsxlxqeggmpqgggtarwyvk
Translated Amino Acid sequence (All Frames) 
Frame A:
ky*iiky*KMSSIGIDLXTTYSCVGVXQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDA
AKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKV
FSPEEIXSMVLLKMKETAEAYLGKTINNAVITXPAYFNDSQRXATKDAGTISKLNVQRII
NEPTAAAIAYG---

---FQEIQQFPCKKTQTFSTYSDNXPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRG
VPQVEVTFDVDANGILNVSAEDKSTGNKQKITIANDKGRLSKEEIEKMVADAEKFKQQDE
HKKIVLNQRINWKIMHSLLKIQLKMKKLQPKFQIQINQLLNLKLKVYSNG*nqikpqkrm
nmkir*kp*kqlsiqimsxlxqeggmpqgggtarwyvk

Frame B:
nik*lnikkchqlvlixvqhihvlvxgkmivlkllqmikvieplhlmlhlliqnv*lxml
qriklq*iqpiqylmlnv*lvxnlvtkkfkvi*nighsklfqrmxishifklnskvklkf
shqkksxqwys*k*kkplklilvkpliml*lpxqlismivnvkqpkmlvqfqn*mfnvll
mnqllllllmv---

---skkynnshvrklkpslhtliixqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqev
yhklklhlmlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmn
tkrsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*
i*r*deslrsscqsrs*vnsikkevchkvvvlpggms

Frame C:
ilnn*ilknvinwy*fxynifmcwcxak*sc*nyck*sr**nhsilcciy*yrtfnwxcc
kesscnesnqysi*c*tfnwsxi**qrssk*yetlaiqsyskgwx*atyss*iqr*n*sf
ltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stxsnqrcwynfkikcstyy*
*tncccyclwf---

---prnttipm*ensnllyil**sxrcinssl*r*tcydqr**fig*i*tfwyststkrc
tts*syi*c*ckwylkcis*r*inw*qtkdyncq**rssfkrrn*kngc*creiqttr*t
qkdrvesknklenyaftvknsikdekvaakisdsdkstiesetesvlkwlesnqtaekde
yedkmkaleavvnpdhextxsrrryatrwwycqvvcq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFD272 (SFD272Q) /CSM/SF/SFD2-C/SFD272Q.Seq.d/                       2313   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1207   0.0
VHK815 (VHK815Q) /CSM/VH/VHK8-A/VHK815Q.Seq.d/                       1207   0.0
VHD617 (VHD617Q) /CSM/VH/VHD6-A/VHD617Q.Seq.d/                       1207   0.0
VHA854 (VHA854Q) /CSM/VH/VHA8-C/VHA854Q.Seq.d/                       1207   0.0
CHR196 (CHR196Q) /CSM/CH/CHR1-D/CHR196Q.Seq.d/                       1207   0.0
CHM141 (CHM141Q) /CSM/CH/CHM1-B/CHM141Q.Seq.d/                       1207   0.0
CHL177 (CHL177Q) /CSM/CH/CHL1-D/CHL177Q.Seq.d/                       1207   0.0
CHI127 (CHI127Q) /CSM/CH/CHI1-B/CHI127Q.Seq.d/                       1207   0.0
CHE894 (CHE894Q) /CSM/CH/CHE8-D/CHE894Q.Seq.d/                       1207   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1067   0.0    5
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.   866   0.0    5
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   228   e-155  7
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   228   e-142  12
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 127   e-109  8
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   127   e-108  8
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          127   e-108  8
AF221541|AF221541.1 Cryptosporidium serpentis heat shock protein 70 (HSP70) gene, partial cds.   139   e-107  11
AF221542|AF221542.1 Cryptosporidium muris isolate 589 heat shock protein 70 (HSP70) gene, partial cds.   103   1e-98  10
AF221543|AF221543.1 Cryptosporidium muris isolate 707 heat shock protein 70 (HSP70) gene, partial cds.   117   9e-98  8
dna update 2003.12.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK097824_1(AK097824|pid:none) Homo sapiens cDNA FLJ40505 fis, cl...   298   8e-98
(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   351   3e-95
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   349   1e-94
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   328   3e-88
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   328   3e-88
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           310   6e-83
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   302   2e-80
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   302   2e-80
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   302   2e-80
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   300   5e-80
protein update 2009. 5.23
PSORT 

 psg: 0.86  gvh: 0.40  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SFD272 is cyt
5' end seq. ID SFD272F
5' end seq. 
>SFD272F.Seq
AAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGATTTANGTACAACA
TATTCATGTGTTGGTGTTNGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGT
AATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTAATTGGNGATGCT
GCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATT
GGTCGNAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTT
ATTCCAAAGGATGGNGATAAGCCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTT
TTCTCACCAGAAGAAATCTNTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCT
TATCTTGGTAAAACCATTAATAATGCTGTAATTACCGNTCCAGCTTATTTCAATGATAGT
CAACGTNAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATT
AATGAACCAACTGCTGCTGCTATTGCTTATGGTTT----------
Length of 5' end seq. 575
3' end seq. ID SFD272Z
3' end seq. 
>SFD272Z.Seq
----------TTCCAAGAAATACAACAATTCCCATGTAAGAAAACTCAAACCTTCTCTAC
ATACTCTGATAATCANCCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGAC
CAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGG
TGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGC
TGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTGCCAATGATAAAGGTCGTCT
TTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGA
ACACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAA
AATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATT
GAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGAT
GAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAGATCATGAGTNAACT
CNATCAAGAAGGAGGTATGCCACAAGGTGGTGGTACTGCCAGGTGGTATGTCAAA
Length of 3' end seq. 645
Connected seq. ID SFD272P
Connected seq. 
>SFD272P.Seq
AAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTGATTTANGTACAACA
TATTCATGTGTTGGTGTTNGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGT
AATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTTTAATTGGNGATGCT
GCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATT
GGTCGNAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTT
ATTCCAAAGGATGGNGATAAGCCACATATTCAAGTTGAATTCAAAGGTGAAACTAAAGTT
TTCTCACCAGAAGAAATCTNTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCT
TATCTTGGTAAAACCATTAATAATGCTGTAATTACCGNTCCAGCTTATTTCAATGATAGT
CAACGTNAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATT
AATGAACCAACTGCTGCTGCTATTGCTTATGGTTT----------TTCCAAGAAATACAA
CAATTCCCATGTAAGAAAACTCAAACCTTCTCTACATACTCTGATAATCANCCAGGTGTA
TTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAA
TTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTT
GATGTTGATGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAA
CAAAAGATTACAATTGCCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATG
GTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACACAAAAAGATCGTGTTGAATCAA
AGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTG
CAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCA
AATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCT
TAGAAGCAGTTGTCAATCCAGATCATGAGTNAACTCNATCAAGAAGGAGGTATGCCACAA
GGTGGTGGTACTGCCAGGTGGTATGTCAAA
Length of connected seq. 1220
Full length Seq ID -
Full length Seq. -
Length of full length seq. -