>AFI116 (AFI116Q) /CSM/AF/AFI1-A/AFI116Q.Seq.d/
ATGTTGCCAATTTCAACCACTCGTCAAGAGATTAAAAGTCATGTTGCAACCTCAAAGGTT
GAGCATAAAATTGAAACATTGGCATCACGTATGACAACTCCATTCATTGATAATGAAGCC
TTATTCACAATACCAATTGATGAAGGTGGTCGTGTTAAAGTTTGGAAAATTGAAGACTAT
GAAAAGATTGATCATCCACAACATTTATATTCTCAATTCTTTTCATCAGATAGTTATATC
GTTCTCTATACCTATATGCAAAATAATAAGGAAGCTCATGTTATCTATTATTATTTAGGT
AGAGATTCTTCAATCAATGAAAAAGGTACTTCCGCTTATTTAACAGTTGATCTCAATGAA
TCATTGGTTGGTGCTTGTGTTCAAACTAGAGTAGTTGCAAATAAAGAATGTAAAAACTTT
TTAAATCTTTTCAAAACCAAAATGGTAATTCATAAAGGTAAATACAATAACTATGATCCA
AAGAAACCATCACTCTATCAAGTTAAAGGTTTAGATAAAATTGATATTCGTGCCGTTCAA
GTAGAATTCTCAAGCTCAATGTTAAATACTTTACATGTTTCAATCTTGCGTACTCCTGAA
AAGATTTACATTTGGCATGGGTAAATTCTCATTGGATGCTGAACAAAACTCTGCTCTXXX
XXXXXXXAGAATTTCAATTCCACTTCAGCTCCAATCGAAATCTTAAAAGAAGGTAGTGAA
TCCAATGAATTTTGGTCAGCTTTTGAAAGTACAGGTGGTAGACAAAAATACTTTAATGAT
ATTATGATTCAAAGTAGTTCAATTCCAACCTCATTCACTTATAAACCACGTTTCTTTGTT
TGTAGTAATGCATCGGGTATCGTTGAAGTTACAGAGGAATCACCATTCTCTCAAGATGAT
CTAGATATTGGATCCGTTTGTATATTGGATGTACAAAGTCATATTTACCTTTGGATTGGT
AGTCGTGCAACTCATCGTACTAAACGTGCCTCTATGGAAGTTGTATTAAACTTTATTGAA
ACTTCAAAATTGGGTCATTCAAAAGAGCACACTAAAGTACTAATTGCAACTCCATTCGAA
GAACCAATTGGTTTCAAAAGTTATTTCCGTGCTTGGTGTACCTCTAAATATCCAAAGAAT
AAATTACCTTTGGTTGAAAAAGATGGTATCCCAGTTGAACAAGTACTTAAAGATTATCTC
AAAGAAATCTATACCTATGAAGAGTTATTGGCCGATCCATTACCTGCTGGCGTTGACTCT
ACTAAATTGGATACTTATCTCAATGATGAAGATTTTGAAAAAGTTTTCAAAATGACTAGA
ACTGAATGGTTAAAGATTCCAGCTGGAAGAGAGAAGGTATTAAAAAAGAATTATTCTTAT
TTTAAAAATAAAAANA

ccqfqplvkrlkvmlqpqrlsiklkhwhhv*qlhslimkpysqyqlmkvvvlkfgklktm
krliihniyilnsfhqivisfsipickiirklmlsiii*veilqsmkkvlpli*qlismn
hwlvlvfkle*lqiknvktf*ifskpkw*fikvntitmiqrnhhsiklkv*iklifvpfk
*nsqaqc*ILYMFQSCVLLKRFTFGMGKFSLDAEQNSA---

---NFNSTSAPIEILKEGSESNEFWSAFESTGGRQKYFNDIMIQSSSIPTSFTYKPRFFV
CSNASGIVEVTEESPFSQDDLDIGSVCILDVQSHIYLWIGSRATHRTKRASMEVVLNFIE
TSKLGHSKEHTKVLIATPFEEPIGFKSYFRAWCTSKYPKNKLPLVEKDGIPVEQVLKDYL
KEIYTYEELLADPLPAGVDSTKLDTYLNDEDFEKVFKMTRTEWLKIPAGREKVLKKNYSY
FKNKX

Frame A:
mlpisttrqeikshvatskvehkietlasrmttpfidnealftipideggrvkvwkiedy
ekidhpqhlysqffssdsyivlytymqnnkeahviyyylgrdssinekgtsayltvdlne
slvgacvqtrvvankecknflnlfktkmvihkgkynnydpkkpslyqvkgldkidiravq
vefsssmlntlhvsilrtpekiyiwhg*iligc*tklcs---

---risiplqlqsks*kkvvnpmnfgqllkvqvvdkntlmil*fkvvqfqphslinhvsl
fvvmhrvslklqrnhhslkmi*ildpfvywmykviftfglvvvqlivlnvplwkly*tll
klqnwviqkstlky*lqlhsknqlvskvisvlgvplniqrinylwlkkmvsqlnkylkii
skksipmksywpihyllaltllnwilismmkilkkfsk*lelng*rfqleerry*kriil
ilkikx

Frame B:
ccqfqplvkrlkvmlqpqrlsiklkhwhhv*qlhslimkpysqyqlmkvvvlkfgklktm
krliihniyilnsfhqivisfsipickiirklmlsiii*veilqsmkkvlpli*qlismn
hwlvlvfkle*lqiknvktf*ifskpkw*fikvntitmiqrnhhsiklkv*iklifvpfk
*nsqaqc*ILYMFQSCVLLKRFTFGMGKFSLDAEQNSA---

---efqfhfssnrnlkrr**iq*ilvsf*kyrw*tkil**yydsk*fnsnlihl*ttflc
l**cigyr*syrgitilsr*srywirlyigctksylpldw*scnssy*tclygscikly*
nfkigsfkrah*stncnsirrtnwfqklfpclvyl*iske*itfg*krwyps*tst*rls
qrnlyl*rvigrsitcwr*ly*igylsq**rf*ksfqnd*n*mvkdsswkregikkelfl
f*k*k

Frame C:
vanfnhssrd*ksccnlkg*a*n*nigitydnsih***slihntn**rwsc*slen*rl*
kd*ssttfifsilfir*lyrslylyak**gsscylllfr*rffnq*kryfrlfns*sq*i
igwclcsn*ssck*rm*klfksfqnqngns*r*iq*l*sketitlss*rfr*n*yscrss
rilklnvkyftcfnlays*kdlhlawvnshwmlnktll---

---NFNSTSAPIEILKEGSESNEFWSAFESTGGRQKYFNDIMIQSSSIPTSFTYKPRFFV
CSNASGIVEVTEESPFSQDDLDIGSVCILDVQSHIYLWIGSRATHRTKRASMEVVLNFIE
TSKLGHSKEHTKVLIATPFEEPIGFKSYFRAWCTSKYPKNKLPLVEKDGIPVEQVLKDYL
KEIYTYEELLADPLPAGVDSTKLDTYLNDEDFEKVFKMTRTEWLKIPAGREKVLKKNYSY
FKNKX

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI116 (AFI116Q) /CSM/AF/AFI1-A/AFI116Q.Seq.d/                       2704   0.0
AHI567 (AHI567Q) /CSM/AH/AHI5-C/AHI567Q.Seq.d/                       1388   0.0
CHI516 (CHI516Q) /CSM/CH/CHI5-A/CHI516Q.Seq.d/                       1386   0.0
SHK802 (SHK802Q) /CSM/SH/SHK8-A/SHK802Q.Seq.d/                       1348   0.0
SHJ849 (SHJ849Q) /CSM/SH/SHJ8-C/SHJ849Q.Seq.d/                       1294   0.0
AHN388 (AHN388Q) /CSM/AH/AHN3-D/AHN388Q.Seq.d/                       1211   0.0
SSB880 (SSB880Q) /CSM/SS/SSB8-D/SSB880Q.Seq.d/                       1120   0.0
AHC823 (AHC823Q) /CSM/AH/AHC8-A/AHC823Q.Seq.d/                       1057   0.0
AHF158 (AHF158Q) /CSM/AH/AHF1-C/AHF158Q.Seq.d/                       1015   0.0
AHD338 (AHD338Q) /CSM/AH/AHD3-B/AHD338Q.Seq.d/                        981   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U78754|U78754.1 Dictyostelium discoideum villin (vilA) mRNA, partial cds.  1342   0.0    4
AJ427856|AJ427856.1 Dictyostelium discoideum ORF encoding villidin.  1342   0.0    4
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    38   2e-05  16
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    36   3e-05  17
BZ393782|BZ393782.1 EINAM68TR EI_10_12_KB Entamoeba invadens genomic clone EINAM68, DNA sequence.    48   9e-05  2
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   0.001  23
AE017338|AE017338.1 Mycoplasma mobile 163K section 2 of 3 of the complete genome.    32   0.007  15
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    48   0.008  13
AP003360|AP003360.3 Staphylococcus aureus subsp. aureus Mu50 DNA, complete genome, section 3/9.    34   0.011  15
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    38   0.012  18

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ427856_1(AJ427856|pid:none) Dictyostelium discoideum ORF encod...   873   0.0
(Q8WQ85) RecName: Full=Villidin;                                      873   0.0
DQ453500_1(DQ453500|pid:none) Heliocidaris tuberculata advillin ...   226   1e-57
EU334660_1(EU334660|pid:none) Strongylocentrotus purpuratus vill...   224   4e-57
AK154851_1(AK154851|pid:none) Mus musculus NOD-derived CD11c +ve...   206   2e-51
BC135895_1(BC135895|pid:none) Xenopus tropicalis hypothetical pr...   201   6e-50
BC111730_1(BC111730|pid:none) Homo sapiens advillin, mRNA (cDNA ...   200   8e-50
AK314362_1(AK314362|pid:none) Homo sapiens cDNA, FLJ95130, Homo ...   200   8e-50
BC054960_1(BC054960|pid:none) Xenopus laevis villin 1, mRNA (cDN...   199   2e-49
(O75366) RecName: Full=Advillin; AltName: Full=p92;  &AF041449_1...   197   5e-49

 psg: 0.90  gvh: 0.47  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for AFI116 is cyt

>AFI116F.Seq
ATGTTGCCAATTTCAACCACTCGTCAAGAGATTAAAAGTCATGTTGCAACCTCAAAGGTT
GAGCATAAAATTGAAACATTGGCATCACGTATGACAACTCCATTCATTGATAATGAAGCC
TTATTCACAATACCAATTGATGAAGGTGGTCGTGTTAAAGTTTGGAAAATTGAAGACTAT
GAAAAGATTGATCATCCACAACATTTATATTCTCAATTCTTTTCATCAGATAGTTATATC
GTTCTCTATACCTATATGCAAAATAATAAGGAAGCTCATGTTATCTATTATTATTTAGGT
AGAGATTCTTCAATCAATGAAAAAGGTACTTCCGCTTATTTAACAGTTGATCTCAATGAA
TCATTGGTTGGTGCTTGTGTTCAAACTAGAGTAGTTGCAAATAAAGAATGTAAAAACTTT
TTAAATCTTTTCAAAACCAAAATGGTAATTCATAAAGGTAAATACAATAACTATGATCCA
AAGAAACCATCACTCTATCAAGTTAAAGGTTTAGATAAAATTGATATTCGTGCCGTTCAA
GTAGAATTCTCAAGCTCAATGTTAAATACTTTACATGTTTCAATCTTGCGTACTCCTGAA
AAGATTTACATTTGGCATGGGTAAATTCTCATTGGATGCTGAACAAAACTCTGCTCT---
-------

>AFI116Z.Seq
----------AGAATTTCAATTCCACTTCAGCTCCAATCGAAATCTTAAAAGAAGGTAGT
GAATCCAATGAATTTTGGTCAGCTTTTGAAAGTACAGGTGGTAGACAAAAATACTTTAAT
GATATTATGATTCAAAGTAGTTCAATTCCAACCTCATTCACTTATAAACCACGTTTCTTT
GTTTGTAGTAATGCATCGGGTATCGTTGAAGTTACAGAGGAATCACCATTCTCTCAAGAT
GATCTAGATATTGGATCCGTTTGTATATTGGATGTACAAAGTCATATTTACCTTTGGATT
GGTAGTCGTGCAACTCATCGTACTAAACGTGCCTCTATGGAAGTTGTATTAAACTTTATT
GAAACTTCAAAATTGGGTCATTCAAAAGAGCACACTAAAGTACTAATTGCAACTCCATTC
GAAGAACCAATTGGTTTCAAAAGTTATTTCCGTGCTTGGTGTACCTCTAAATATCCAAAG
AATAAATTACCTTTGGTTGAAAAAGATGGTATCCCAGTTGAACAAGTACTTAAAGATTAT
CTCAAAGAAATCTATACCTATGAAGAGTTATTGGCCGATCCATTACCTGCTGGCGTTGAC
TCTACTAAATTGGATACTTATCTCAATGATGAAGATTTTGAAAAAGTTTTCAAAATGACT
AGAACTGAATGGTTAAAGATTCCAGCTGGAAGAGAGAAGGTATTAAAAAAGAATTATTCT
TATTTTAAAAATAAAAANA

>AFI116P.Seq
ATGTTGCCAATTTCAACCACTCGTCAAGAGATTAAAAGTCATGTTGCAACCTCAAAGGTT
GAGCATAAAATTGAAACATTGGCATCACGTATGACAACTCCATTCATTGATAATGAAGCC
TTATTCACAATACCAATTGATGAAGGTGGTCGTGTTAAAGTTTGGAAAATTGAAGACTAT
GAAAAGATTGATCATCCACAACATTTATATTCTCAATTCTTTTCATCAGATAGTTATATC
GTTCTCTATACCTATATGCAAAATAATAAGGAAGCTCATGTTATCTATTATTATTTAGGT
AGAGATTCTTCAATCAATGAAAAAGGTACTTCCGCTTATTTAACAGTTGATCTCAATGAA
TCATTGGTTGGTGCTTGTGTTCAAACTAGAGTAGTTGCAAATAAAGAATGTAAAAACTTT
TTAAATCTTTTCAAAACCAAAATGGTAATTCATAAAGGTAAATACAATAACTATGATCCA
AAGAAACCATCACTCTATCAAGTTAAAGGTTTAGATAAAATTGATATTCGTGCCGTTCAA
GTAGAATTCTCAAGCTCAATGTTAAATACTTTACATGTTTCAATCTTGCGTACTCCTGAA
AAGATTTACATTTGGCATGGGTAAATTCTCATTGGATGCTGAACAAAACTCTGCTCT---
-------AGAATTTCAATTCCACTTCAGCTCCAATCGAAATCTTAAAAGAAGGTAGTGAA
TCCAATGAATTTTGGTCAGCTTTTGAAAGTACAGGTGGTAGACAAAAATACTTTAATGAT
ATTATGATTCAAAGTAGTTCAATTCCAACCTCATTCACTTATAAACCACGTTTCTTTGTT
TGTAGTAATGCATCGGGTATCGTTGAAGTTACAGAGGAATCACCATTCTCTCAAGATGAT
CTAGATATTGGATCCGTTTGTATATTGGATGTACAAAGTCATATTTACCTTTGGATTGGT
AGTCGTGCAACTCATCGTACTAAACGTGCCTCTATGGAAGTTGTATTAAACTTTATTGAA
ACTTCAAAATTGGGTCATTCAAAAGAGCACACTAAAGTACTAATTGCAACTCCATTCGAA
GAACCAATTGGTTTCAAAAGTTATTTCCGTGCTTGGTGTACCTCTAAATATCCAAAGAAT
AAATTACCTTTGGTTGAAAAAGATGGTATCCCAGTTGAACAAGTACTTAAAGATTATCTC
AAAGAAATCTATACCTATGAAGAGTTATTGGCCGATCCATTACCTGCTGGCGTTGACTCT
ACTAAATTGGATACTTATCTCAATGATGAAGATTTTGAAAAAGTTTTCAAAATGACTAGA
ACTGAATGGTTAAAGATTCCAGCTGGAAGAGAGAAGGTATTAAAAAAGAATTATTCTTAT
TTTAAAAATAAAAANA