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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFD257
Library SF
(Link to library)
Clone ID SFD257
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15009-1
Original site URL
Representative seq. ID SFD257P
(Link to Original site)
Representative DNA sequence 
>SFD257 (SFD257Q) /CSM/SF/SFD2-C/SFD257Q.Seq.d/
ATTTATATATATACACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATT
TTATCTTGTATTTTTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAAT
TTCAAATGGTGGGGCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAA
TGGTAAAGCGGATTATAATGTTGAACATTTTTTCAGAGGTGAACATACAAATAATTGGGC
ACTTTTAGTTTGCACATCAAGATTTTGGTTTAACTATAGACATATAGCAAATGTATTAGG
TTTTTATAGAACTGTTAAGAAATTAGGTATACCTGACTCACAAATTATATTAATGTTAGC
AGATGATATGGCATGTAATCCTAGAAATTCATATGCTGGTAGTATATTCAATAATGAAAA
TCATAAATTGAATTTATATGGTGATAATATTGAAGTTGACTATAGAGGATATGAAGTTAA
TGTTGAAAATTTCATTAGAGTGTTAACTGGTAGACATGAACCAGAGGTAGCACGTTCAAA
GAGATTATTGACAGATGATAAAAGTAATATTTTAATTTTCTTAACTGGXXXXXXXXXXGA
AAAGATATCATGAAATTTTATTCATGGTTGATACTTGTCAAGCTAATACATTATATACTC
GTTTTAATTCTCCAAATATTTTAGCAATTGGTTCAAGTAAATTTGGTGAAAATAGTTATT
CTCATCATTCAGATCCAGAATTAGGGGTTGCAGTTATTGATAGATTTACATATTATACAT
TGGAATTCTTTGAAAGTGTTGACCCACATAATGTAACATTATATCAATTGTTCAATACAT
ATAGTCCACAAAAATTACAATCACATAGTGAATATAGGACAGATTTATTCAATAGACCAT
TAGATCAAGTTCCTGTTACAGACTTTTTTGGTTCTGTAATGAGAGCTGATTTAACTCCTT
CAACAATTGTAAATGAAAAACAAAAAGATGAAAAAGTAGAAAAAGATGAAACTACTGTAA
ATAATAATAATAATAATAACAACGATAATGTAAAAAAACAAACACCAAAAACTAAACAAA
TTTCATATAGAATTACAGAGAAACCAACATCAATCCAAGTACAAAATAACAAAGATAGTG
AATTAAATCTTTTCAATTCAAAATTTATTACTTCAGCTTTAATATTTTTAGTAGTATTTG
GATTACTTTCTTGGTCTTCTGATTTTGTAAAATCAAATCTTATTTTAAATACAAATNATT
TAAAAGATCTGAAATCAAAATCAAAGCAAATTTAAGTAATATATAAATAAATAAATAATA
AATAAATTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
fiyihttikk*KMKKTTFRFYLVFLYAILVLYIGGIEDAISNGGAMGYEQPKTNFYNGGN
GKADYNVEHFFRGEHTNNWALLVCTSRFWFNYRHIANVLGFYRTVKKLGIPDSQIILMLA
DDMACNPRNSYAGSIFNNENHKLNLYGDNIEVDYRGYEVNVENFIRVLTGRHEPEVARSK
RLLTDDKSNILIFLT---

---KRYHEILFMVDTCQANTLYTRFNSPNILAIGSSKFGENSYSHHSDPELGVAVIDRFT
YYTLEFFESVDPHNVTLYQLFNTYSPQKLQSHSEYRTDLFNRPLDQVPVTDFFGSVMRAD
LTPSTIVNEKQKDEKVEKDETTVNNNNNNNNDNVKKQTPKTKQISYRITEKPTSIQVQNN
KDSELNLFNSKFITSALIFLVVFGLLSWSSDFVKSNLILNTNXLKDLKSKSKQI*viyk*
innk*i
Translated Amino Acid sequence (All Frames) 
Frame A:
iyiythnnkeikdekndi*ilscificnisfiyrwy*rcnfkwwgdgi*ttknkll*ww*
w*sgl*c*tffqr*tyk*lgtfslhikilv*l*tyskcirfl*nc*eiryt*ltnyinvs
r*ygm*s*kficw*yiq**ks*iefiw**y*s*l*ri*s*c*kfh*svnw*t*trgstfk
eiidr**k*yfnflnw---

---ekis*nfihg*ylss*yiiysf*fskyfsnwfk*iw*k*lfssfrsrirgcsy**iy
ilyigil*kc*pt*cniisivqyi*stkitit**i*drfiq*tirssscyrlfwfcnes*
fnsfnnck*ktkr*ksrkr*nyck******qr*ckktntkn*tnfi*nyretninpstk*
qr**iksfqfkiyyfsfnifssiwitflvf*fckiksyfkykxfkrseikikanlsni*i
nk**in

Frame B:
fiyihttikk*KMKKTTFRFYLVFLYAILVLYIGGIEDAISNGGAMGYEQPKTNFYNGGN
GKADYNVEHFFRGEHTNNWALLVCTSRFWFNYRHIANVLGFYRTVKKLGIPDSQIILMLA
DDMACNPRNSYAGSIFNNENHKLNLYGDNIEVDYRGYEVNVENFIRVLTGRHEPEVARSK
RLLTDDKSNILIFLT---

---KRYHEILFMVDTCQANTLYTRFNSPNILAIGSSKFGENSYSHHSDPELGVAVIDRFT
YYTLEFFESVDPHNVTLYQLFNTYSPQKLQSHSEYRTDLFNRPLDQVPVTDFFGSVMRAD
LTPSTIVNEKQKDEKVEKDETTVNNNNNNNNDNVKKQTPKTKQISYRITEKPTSIQVQNN
KDSELNLFNSKFITSALIFLVVFGLLSWSSDFVKSNLILNTNXLKDLKSKSKQI*viyk*
innk*i

Frame C:
lyiytqq*rnkr*kkrhldfilyfymqy*fyi*vvlkmqfqmvgrwdmnnqkqtfimvvm
vkriimlnifsevniqiighf*fahqdfgltidi*qmy*vfiellrn*vylthkly*c*q
miwhvileihmlvvysimkiin*iymviilkltiedmklmlkislec*lvdmnqr*hvqr
dy*qmikvif*fs*l---

---kdimkfyswlilvklihyilvlilqif*qlvqvnlvkiviliiqiqn*glqllidlh
iihwnslkvlthim*hyincsihivhknynhivnigqiysidh*ikfllqtflvl**eli
*llqql*mknkkmkk*kkmkll*iiiiiittim*knkhqklnkfhielqrnqhqskykit
kivn*ifsiqnlllql*yf**yldyflgllil*nqilf*iqxi*ki*nqnqskfk*yink
*iinkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFD257 (SFD257Q) /CSM/SF/SFD2-C/SFD257Q.Seq.d/                       1822   0.0
SFL282 (SFL282Q) /CSM/SF/SFL2-D/SFL282Q.Seq.d/                       1116   0.0
CFB364 (CFB364Q) /CSM/CF/CFB3-C/CFB364Q.Seq.d/                       1108   0.0
AFJ746 (AFJ746Q) /CSM/AF/AFJ7-B/AFJ746Q.Seq.d/                       1108   0.0
SFJ248 (SFJ248Q) /CSM/SF/SFJ2-B/SFJ248Q.Seq.d/                       1100   0.0
CFH296 (CFH296Q) /CSM/CF/CFH2-D/CFH296Q.Seq.d/                       1096   0.0
CFF820 (CFF820Q) /CSM/CF/CFF8-A/CFF820Q.Seq.d/                       1082   0.0
CFH352 (CFH352Q) /CSM/CF/CFH3-C/CFH352Q.Seq.d/                        936   0.0
CHD461 (CHD461Q) /CSM/CH/CHD4-C/CHD461Q.Seq.d/                        928   0.0
VFB704 (VFB704Q) /CSM/VF/VFB7-A/VFB704Q.Seq.d/                        763   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI507800|BI507800.1 BB170001B10H11.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170001B10H11 5', mRNA sequence.    68   3e-35  6
BI515788|BI515788.1 BB160020B10A08 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160020B10A08 5', mRNA sequence.    68   9e-24  5
BI515765|BI515765.1 BB160020A20B07 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160020A20B07 5', mRNA sequence.    68   1e-21  4
AX488980|AX488980.1 Sequence 6280 from Patent WO02053728.          52   2e-16  4
BU835350|BU835350.1 T072G09 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    40   1e-08  3
BU824990|BU824990.1 UK102C04 Populus apical shoot cDNA library Populus tremula x Populus tremuloides cDNA 5 prime, mRNA sequence.    40   1e-08  3
AL871211|AL871211.2 Xenopus tropicalis EST, clone TEgg119k23 5'.    58   4e-07  3
AC133213|AC133213.2 Mus musculus chromosome UL clone RP23-60I24, complete sequence.    38   1e-06  8
AC111088|AC111088.4 Mus musculus clone RP23-154M15, WORKING DRAFT SEQUENCE, 3 ordered pieces.    40   2e-06  10
AC118645|AC118645.11 Mus musculus chromosome 15 clone RP24-114E9 map 15, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.    38   4e-06  9
dna update 2003.12.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR382132_329(CR382132|pid:none) Yarrowia lipolytica strain CLIB1...   193   1e-87
AK117596_1(AK117596|pid:none) Arabidopsis thaliana At1g08750 mRN...   210   2e-87
AY084883_1(AY084883|pid:none) Arabidopsis thaliana clone 120167 ...   210   2e-87
(P49018) RecName: Full=GPI-anchor transamidase;          Short=G...   190   1e-83
CP000500_66(CP000500|pid:none) Pichia stipitis CBS 6054 chromoso...   195   1e-83
CR382138_1120(CR382138|pid:none) Debaryomyces hansenii strain CB...   191   3e-83
CR382138_1225(CR382138|pid:none) Debaryomyces hansenii chromosom...   189   1e-82
FM992688_685(FM992688|pid:none) Candida dubliniensis CD36 chromo...   188   5e-82
AE016817_700(AE016817|pid:none) Ashbya gossypii (= Eremothecium ...   184   2e-81
CR860289_1(CR860289|pid:none) Pongo abelii mRNA; cDNA DKFZp459A1...   202   4e-80
protein update 2009. 5.23
PSORT 

 psg: 0.95  gvh: 0.58  alm: 0.24  top: 0.00  tms: 0.13  mit: 0.37  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 24.0 %: mitochondrial
	 12.0 %: plasma membrane
	  8.0 %: cytoplasmic
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for SFD257 is end
5' end seq. ID SFD257F
5' end seq. 
>SFD257F.Seq
ATTTATATATATACACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATT
TTATCTTGTATTTTTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAAT
TTCAAATGGTGGGGCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAA
TGGTAAAGCGGATTATAATGTTGAACATTTTTTCAGAGGTGAACATACAAATAATTGGGC
ACTTTTAGTTTGCACATCAAGATTTTGGTTTAACTATAGACATATAGCAAATGTATTAGG
TTTTTATAGAACTGTTAAGAAATTAGGTATACCTGACTCACAAATTATATTAATGTTAGC
AGATGATATGGCATGTAATCCTAGAAATTCATATGCTGGTAGTATATTCAATAATGAAAA
TCATAAATTGAATTTATATGGTGATAATATTGAAGTTGACTATAGAGGATATGAAGTTAA
TGTTGAAAATTTCATTAGAGTGTTAACTGGTAGACATGAACCAGAGGTAGCACGTTCAAA
GAGATTATTGACAGATGATAAAAGTAATATTTTAATTTTCTTAACTGG----------
Length of 5' end seq. 588
3' end seq. ID SFD257Z
3' end seq. 
>SFD257Z.Seq
----------GAAAAGATATCATGAAATTTTATTCATGGTTGATACTTGTCAAGCTAATA
CATTATATACTCGTTTTAATTCTCCAAATATTTTAGCAATTGGTTCAAGTAAATTTGGTG
AAAATAGTTATTCTCATCATTCAGATCCAGAATTAGGGGTTGCAGTTATTGATAGATTTA
CATATTATACATTGGAATTCTTTGAAAGTGTTGACCCACATAATGTAACATTATATCAAT
TGTTCAATACATATAGTCCACAAAAATTACAATCACATAGTGAATATAGGACAGATTTAT
TCAATAGACCATTAGATCAAGTTCCTGTTACAGACTTTTTTGGTTCTGTAATGAGAGCTG
ATTTAACTCCTTCAACAATTGTAAATGAAAAACAAAAAGATGAAAAAGTAGAAAAAGATG
AAACTACTGTAAATAATAATAATAATAATAACAACGATAATGTAAAAAAACAAACACCAA
AAACTAAACAAATTTCATATAGAATTACAGAGAAACCAACATCAATCCAAGTACAAAATA
ACAAAGATAGTGAATTAAATCTTTTCAATTCAAAATTTATTACTTCAGCTTTAATATTTT
TAGTAGTATTTGGATTACTTTCTTGGTCTTCTGATTTTGTAAAATCAAATCTTATTTTAA
ATACAAATNATTTAAAAGATCTGAAATCAAAATCAAAGCAAATTTAAGTAATATATAAAT
AAATAAATAATAAATAAATTA
Length of 3' end seq. 731
Connected seq. ID SFD257P
Connected seq. 
>SFD257P.Seq
ATTTATATATATACACACAACAATAAAGAAATAAAAGATGAAAAAAACGACATTTAGATT
TTATCTTGTATTTTTATATGCAATATTAGTTTTATATATAGGTGGTATTGAAGATGCAAT
TTCAAATGGTGGGGCGATGGGATATGAACAACCAAAAACAAACTTTTATAATGGTGGTAA
TGGTAAAGCGGATTATAATGTTGAACATTTTTTCAGAGGTGAACATACAAATAATTGGGC
ACTTTTAGTTTGCACATCAAGATTTTGGTTTAACTATAGACATATAGCAAATGTATTAGG
TTTTTATAGAACTGTTAAGAAATTAGGTATACCTGACTCACAAATTATATTAATGTTAGC
AGATGATATGGCATGTAATCCTAGAAATTCATATGCTGGTAGTATATTCAATAATGAAAA
TCATAAATTGAATTTATATGGTGATAATATTGAAGTTGACTATAGAGGATATGAAGTTAA
TGTTGAAAATTTCATTAGAGTGTTAACTGGTAGACATGAACCAGAGGTAGCACGTTCAAA
GAGATTATTGACAGATGATAAAAGTAATATTTTAATTTTCTTAACTGG----------GA
AAAGATATCATGAAATTTTATTCATGGTTGATACTTGTCAAGCTAATACATTATATACTC
GTTTTAATTCTCCAAATATTTTAGCAATTGGTTCAAGTAAATTTGGTGAAAATAGTTATT
CTCATCATTCAGATCCAGAATTAGGGGTTGCAGTTATTGATAGATTTACATATTATACAT
TGGAATTCTTTGAAAGTGTTGACCCACATAATGTAACATTATATCAATTGTTCAATACAT
ATAGTCCACAAAAATTACAATCACATAGTGAATATAGGACAGATTTATTCAATAGACCAT
TAGATCAAGTTCCTGTTACAGACTTTTTTGGTTCTGTAATGAGAGCTGATTTAACTCCTT
CAACAATTGTAAATGAAAAACAAAAAGATGAAAAAGTAGAAAAAGATGAAACTACTGTAA
ATAATAATAATAATAATAACAACGATAATGTAAAAAAACAAACACCAAAAACTAAACAAA
TTTCATATAGAATTACAGAGAAACCAACATCAATCCAAGTACAAAATAACAAAGATAGTG
AATTAAATCTTTTCAATTCAAAATTTATTACTTCAGCTTTAATATTTTTAGTAGTATTTG
GATTACTTTCTTGGTCTTCTGATTTTGTAAAATCAAATCTTATTTTAAATACAAATNATT
TAAAAGATCTGAAATCAAAATCAAAGCAAATTTAAGTAATATATAAATAAATAAATAATA
AATAAATTA
Length of connected seq. 1319
Full length Seq ID -
Full length Seq. -
Length of full length seq. -