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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFD228
Library SF
(Link to library)
Clone ID SFD228
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16366-1
Original site URL
Representative seq. ID SFD228P
(Link to Original site)
Representative DNA sequence 
>SFD228 (SFD228Q) /CSM/SF/SFD2-B/SFD228Q.Seq.d/
ACACCAAACGTTTAAAATTATTAGACTCAATCGCTGGTAGTCAATTCATTATAAACACAT
TGGATCAACGTAAACTTTGGGTCAATCATGAGAAAGGTGATATAATTAAGCAAGGTGATA
TGCGTTACATAGAGAATGAAGGTATGCCAATTAAAGGTACCTCTAGAAAAGGTAAATTAA
TCATTGCTTTTGATATTGAATATCCATCGAATCTTACAAATGATGATATTGAAAAACTCT
CTAAAATCTTACCAAAAGCTGCCACCCCTTCTGTTTCAAAATCTGATTGTAAATCGGTTG
GTCTCTCAAAGGTAAACTTTAATACCAACGAACAAAGTTCTCATGGTGGTGCTGGTGGTG
CTTATCAACAACATGGTGGTGCTTATGGTCATCAAAAACAACAACAACAAGGTTTTAATC
CTGCTGATTTTGGTGCTCAATTTGGTGGTGGTGGTCCACAACAAGCACAACAGTGTCAAC
AACAATAAACAATTTAAAAAAAAAAXXXXXXXXXXACACCAAACGTTTAAAATTATTAGA
CTCAATCGCTGGTAGTCAATTCATTATAAACACATTGGATCAACGTAAACTTTGGGTCAA
TCATGAGAAAGGTGATATAATTAAGCAAGGTGATATGCGTTACATAGAGAATGAAGGTAT
GCCAATTAAAGGTACCTCTAGAAAAGGTAAATTAATCATTGCTTTTGATATTGAATATCC
ATCGAATCTTACAAATGATGATATTGAAAAACTCTCTAAAATCTTACCAAAAGCTGCCAC
CCCTTCTGTTTCAAAATCTGATTGTAAATCGGTTGGTCTCTCAAAGGTAAACTTTAATAC
CAACGAACAAAGTTCTCATGGTGGTGCTGGTGGTGCTTATCAACAACATGGTGGTGCTTA
TGGTCATCAAAAACAACAACAACAAGGTTTTAATCCTGCTGATTTTGGTGCTCAATTTGG
TGGTGGGGTCCACAACAAGCACAACAGTGTCAACAACAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tpnv*ny*tqslvvnsl*thwinvnfgsimrkvi*lskvicvt*rmkvcqlkvplekvn*
slllilnihrilqmmilknslksyqklppllfqnlivnrlvsqr*TLIPTNKVLMVVLVV
LINNMVVLMVIKNNNNKVLILLILVLNLVVVVHNKHNSVNNNKQFKKK---

---TKRLKLLDSIAGSQFIINTLDQRKLWVNHEKGDIIKQGDMRYIENEGMPIKGTSRKG
KLIIAFDIEYPSNLTNDDIEKLSKILPKAATPSVSKSDCKSVGLSKVNFNTNEQSSHGGA
GGAYQQHGGAYGHQKQQQQGFNPADFGAQFGGGVHNKHNSVNNN
Translated Amino Acid sequence (All Frames) 
Frame A:
tpnv*ny*tqslvvnsl*thwinvnfgsimrkvi*lskvicvt*rmkvcqlkvplekvn*
slllilnihrilqmmilknslksyqklppllfqnlivnrlvsqr*TLIPTNKVLMVVLVV
LINNMVVLMVIKNNNNKVLILLILVLNLVVVVHNKHNSVNNNKQFKKK---

---tpnv*ny*tqslvvnsl*thwinvnfgsimrkvi*lskvicvt*rmkvcqlkvplek
vn*slllilnihrilqmmilknslksyqklppllfqnlivnrlvsqr*tliptnkvlmvv
lvvlinnmvvlmviknnnnkvlillilvlnlvvgsttsttvstt

Frame B:
hqtfkiirlnrw*sihykhigst*tlgqs*er*yn*ar*yalhre*ryan*ryl*kr*in
hcf*y*isiesyk**y*ktl*nltkschpfcfki*l*igwslkgkl*yqrtkfswwcwwc
lsttwwclwsskttttrf*sc*fwcsiwwwwsttsttvsttinnlkkk---

---hqtfkiirlnrw*sihykhigst*tlgqs*er*yn*ar*yalhre*ryan*ryl*kr
*inhcf*y*isiesyk**y*ktl*nltkschpfcfki*l*igwslkgkl*yqrtkfswwc
wwclsttwwclwsskttttrf*sc*fwcsiwwwgpqqaqqcqqq

Frame C:
tkrlklldsiagsqfiintldqrklwvnhekgdiikqgdmryienegmpikgtsrkgkli
iafdieypsnltnddieklskilpkaatpsvsksdcksvglskvnfntneqsshggagga
yqqhggayghqkqqqqgfnpadfgaqfggggpqqaqqcqqq*ti*kk---

---TKRLKLLDSIAGSQFIINTLDQRKLWVNHEKGDIIKQGDMRYIENEGMPIKGTSRKG
KLIIAFDIEYPSNLTNDDIEKLSKILPKAATPSVSKSDCKSVGLSKVNFNTNEQSSHGGA
GGAYQQHGGAYGHQKQQQQGFNPADFGAQFGGGVHNKHNSVNNN
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLI160 (SLI160Q) /CSM/SL/SLI1-C/SLI160Q.Seq.d/                        981   0.0
SLE413 (SLE413Q) /CSM/SL/SLE4-A/SLE413Q.Seq.d/                        981   0.0
SLE114 (SLE114Q) /CSM/SL/SLE1-A/SLE114Q.Seq.d/                        981   0.0
SLA810 (SLA810Q) /CSM/SL/SLA8-A/SLA810Q.Seq.d/                        981   0.0
SFD228 (SFD228Q) /CSM/SF/SFD2-B/SFD228Q.Seq.d/                        981   0.0
SLH333 (SLH333Q) /CSM/SL/SLH3-B/SLH333Q.Seq.d/                        975   0.0
SFB251 (SFB251Q) /CSM/SF/SFB2-C/SFB251Q.Seq.d/                        955   0.0
CHC270 (CHC270Q) /CSM/CH/CHC2-C/CHC270Q.Seq.d/                        955   0.0
SFE772 (SFE772Q) /CSM/SF/SFE7-C/SFE772Q.Seq.d/                        954   0.0
CFJ662 (CFJ662Q) /CSM/CF/CFJ6-C/CFJ662Q.Seq.d/                        944   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BX842687|BX842687.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    36   2e-06  8
AC127682|AC127682.2 Mus musculus chromosome UNK clone RP24-170O17, WORKING DRAFT SEQUENCE, 9 unordered pieces.    38   3e-06  7
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    38   2e-05  9
AL929223|AL929223.5 Mouse DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP23-145P1.    38   2e-05  12
AC116925|AC116925.2 Dictyostelium discoideum chromosome 2 map 4189423-4228998 strain AX4, complete sequence.    38   2e-05  6
AL254753|AL254753.1 Tetraodon nigroviridis genome survey sequence PUC-Ori end of clone  045I18 of library G from Tetraodon nigroviridis.    34   4e-05  5
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    36   4e-05  8
AC116920|AC116920.2 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    40   5e-05  7
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.    36   6e-05  10
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    40   6e-05  11
dna update 2003.12.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54ED3) RecName: Full=DnaJ homolog subfamily A member 1 homolog;     238   2e-61
L36113_1(L36113|pid:none) Arabidopsis thaliana chaperone protein...    70   8e-11
(P42825) RecName: Full=Chaperone protein dnaJ 2;          Short=...    70   1e-10
(Q94AW8) RecName: Full=Chaperone protein dnaJ 3;          Short=...    69   2e-10
S71199(S71199) dnaJ protein homolog atj3 - Arabidopsis thaliana ...    69   2e-10
AY045655_1(AY045655|pid:none) Arabidopsis thaliana AT3g44110/F26...    69   2e-10
BT078637_1(BT078637|pid:none) Lepeophtheirus salmonis clone lsal...    69   3e-10
BC046660_1(BC046660|pid:none) Xenopus laevis similar to DnaJ (Hs...    68   4e-10
AF239932_1(AF239932|pid:none) Euphorbia esula DnaJ protein mRNA,...    67   1e-09
AM429733_2(AM429733|pid:none) Vitis vinifera contig VV78X195295....    66   2e-09
protein update 2009. 5.23
PSORT 

 psg: 0.99  gvh: 0.55  alm: 0.16  top: 0.53  tms: 0.13  mit: 0.36  mip: 0.12
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 24.0 %: endoplasmic reticulum
	 20.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: cytoplasmic
	  4.0 %: vesicles of secretory system
	  4.0 %: nuclear

>> prediction for SFD228 is mit
5' end seq. ID SFD228F
5' end seq. 
>SFD228F.Seq
ACACCAAACGTTTAAAATTATTAGACTCAATCGCTGGTAGTCAATTCATTATAAACACAT
TGGATCAACGTAAACTTTGGGTCAATCATGAGAAAGGTGATATAATTAAGCAAGGTGATA
TGCGTTACATAGAGAATGAAGGTATGCCAATTAAAGGTACCTCTAGAAAAGGTAAATTAA
TCATTGCTTTTGATATTGAATATCCATCGAATCTTACAAATGATGATATTGAAAAACTCT
CTAAAATCTTACCAAAAGCTGCCACCCCTTCTGTTTCAAAATCTGATTGTAAATCGGTTG
GTCTCTCAAAGGTAAACTTTAATACCAACGAACAAAGTTCTCATGGTGGTGCTGGTGGTG
CTTATCAACAACATGGTGGTGCTTATGGTCATCAAAAACAACAACAACAAGGTTTTAATC
CTGCTGATTTTGGTGCTCAATTTGGTGGTGGTGGTCCACAACAAGCACAACAGTGTCAAC
AACAATAAACAATTTAAAAAAAAAA----------
Length of 5' end seq. 505
3' end seq. ID SFD228Z
3' end seq. 
>SFD228Z.Seq
----------ACACCAAACGTTTAAAATTATTAGACTCAATCGCTGGTAGTCAATTCATT
ATAAACACATTGGATCAACGTAAACTTTGGGTCAATCATGAGAAAGGTGATATAATTAAG
CAAGGTGATATGCGTTACATAGAGAATGAAGGTATGCCAATTAAAGGTACCTCTAGAAAA
GGTAAATTAATCATTGCTTTTGATATTGAATATCCATCGAATCTTACAAATGATGATATT
GAAAAACTCTCTAAAATCTTACCAAAAGCTGCCACCCCTTCTGTTTCAAAATCTGATTGT
AAATCGGTTGGTCTCTCAAAGGTAAACTTTAATACCAACGAACAAAGTTCTCATGGTGGT
GCTGGTGGTGCTTATCAACAACATGGTGGTGCTTATGGTCATCAAAAACAACAACAACAA
GGTTTTAATCCTGCTGATTTTGGTGCTCAATTTGGTGGTGGGGTCCACAACAAGCACAAC
AGTGTCAACAACAAT
Length of 3' end seq. 485
Connected seq. ID SFD228P
Connected seq. 
>SFD228P.Seq
ACACCAAACGTTTAAAATTATTAGACTCAATCGCTGGTAGTCAATTCATTATAAACACAT
TGGATCAACGTAAACTTTGGGTCAATCATGAGAAAGGTGATATAATTAAGCAAGGTGATA
TGCGTTACATAGAGAATGAAGGTATGCCAATTAAAGGTACCTCTAGAAAAGGTAAATTAA
TCATTGCTTTTGATATTGAATATCCATCGAATCTTACAAATGATGATATTGAAAAACTCT
CTAAAATCTTACCAAAAGCTGCCACCCCTTCTGTTTCAAAATCTGATTGTAAATCGGTTG
GTCTCTCAAAGGTAAACTTTAATACCAACGAACAAAGTTCTCATGGTGGTGCTGGTGGTG
CTTATCAACAACATGGTGGTGCTTATGGTCATCAAAAACAACAACAACAAGGTTTTAATC
CTGCTGATTTTGGTGCTCAATTTGGTGGTGGTGGTCCACAACAAGCACAACAGTGTCAAC
AACAATAAACAATTTAAAAAAAAAA----------ACACCAAACGTTTAAAATTATTAGA
CTCAATCGCTGGTAGTCAATTCATTATAAACACATTGGATCAACGTAAACTTTGGGTCAA
TCATGAGAAAGGTGATATAATTAAGCAAGGTGATATGCGTTACATAGAGAATGAAGGTAT
GCCAATTAAAGGTACCTCTAGAAAAGGTAAATTAATCATTGCTTTTGATATTGAATATCC
ATCGAATCTTACAAATGATGATATTGAAAAACTCTCTAAAATCTTACCAAAAGCTGCCAC
CCCTTCTGTTTCAAAATCTGATTGTAAATCGGTTGGTCTCTCAAAGGTAAACTTTAATAC
CAACGAACAAAGTTCTCATGGTGGTGCTGGTGGTGCTTATCAACAACATGGTGGTGCTTA
TGGTCATCAAAAACAACAACAACAAGGTTTTAATCCTGCTGATTTTGGTGCTCAATTTGG
TGGTGGGGTCCACAACAAGCACAACAGTGTCAACAACAAT
Length of connected seq. 990
Full length Seq ID -
Full length Seq. -
Length of full length seq. -