>AFI111 (AFI111Q) /CSM/AF/AFI1-A/AFI111Q.Seq.d/
XXXXXXXXXXCTCTACTACTGGTAGTACTGAAGGTGCTCATGCATTAAAAACTAAAAAAA
TTGGTTTCACTATCTGAACAAAATTTAGTTGATTGCTCTGGTCCAGAAGAAAACTTTGGT
TGTGATGGTGGTTTAATGAATAATGCATTTGATTATATCATTAAAAACAAGGGTATTGAC
ACTGAAAGTAGTTATCCATATACTGCTGAAACTGGTTCAACATGTTTATTCAATAAATCT
GATATTGGTGCAACCATTAAAGGTTATGTTAACATTACTGCCGGTTCAGAGATTTCATTA
GAGAATGGTGCTCAACATGGTCCAGTATCTGTTGCAATTGATGCTTCCCATAATAGTTTC
CAACTCTATACATCTGGTATCTATTATGAGCCAAAATGTTCTCCAACTGAATTGGATCAT
GGTGTTTTAGTTGTTGGTTATGGTGTTCAAGGTAAAGATGATGAAGGTCCAGTATTAAAC
AGAAAACAAACCATTGTTATCCACAAGAATGAAGATAATAAAGTTGAATCCTCTGATGAT
TCATCTGATAGTGTTAGACCAAAGGCAAATAATTATTGGATCGTTAAAAATTCATGGGGT
ACCTCATGGGGTATAAAAGGTTACATTTTAATGTCAAAGGATAGNACANNANAATTGTGG
TANCGCATCAGTT

---lllvvlkvlmh*KLKKLVSLSEQNLVDCSGPEENFGCDGGLMNNAFDYIIKNKGIDT
ESSYPYTAETGSTCLFNKSDIGATIKGYVNITAGSEISLENGAQHGPVSVAIDASHNSFQ
LYTSGIYYEPKCSPTELDHGVLVVGYGVQGKDDEGPVLNRKQTIVIHKNEDNKVESSDDS
SDSVRPKANNYWIVKNSWGTSWGIKGYILMSKDXTXXLWXRIS

Frame A:
---sttgstegahalktkkigfti*tkfs*llwsrrklwl*wwfne*ci*lyh*kqgy*h
*k*lsiyc*nwfnmfiq*i*ywcnh*rlc*hycrfrdfirewcstwssiccn*cfp**fp
tlyiwyll*akmfsn*igswcfscwlwcsr*r**rssikqktnhcypqe*r**s*il**f
i**c*tkgk*lldr*kfmgylmgykrlhfnvkg*xxxivvxhq


Frame B:
---lllvvlkvlmh*KLKKLVSLSEQNLVDCSGPEENFGCDGGLMNNAFDYIIKNKGIDT
ESSYPYTAETGSTCLFNKSDIGATIKGYVNITAGSEISLENGAQHGPVSVAIDASHNSFQ
LYTSGIYYEPKCSPTELDHGVLVVGYGVQGKDDEGPVLNRKQTIVIHKNEDNKVESSDDS
SDSVRPKANNYWIVKNSWGTSWGIKGYILMSKDXTXXLWXRIS


Frame C:
---yyw*y*rcscikn*knwfhylnki*lialvqkktlvvmvv**imhliislktrvltl
kvvihillklvqhvysinlilvqplkvmltllpvqrfh*rmvlnmvqyllqlmlpiivsn
sihlvsimsqnvlqlnwimvf*llvmvfkvkmmkvqy*tenkpllstrmkiiklnplmih
livldqrqiiigslkihgvphgv*kvtf*cqrixxxncgxasv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFI111 (AFI111Q) /CSM/AF/AFI1-A/AFI111Q.Seq.d/                       1185   0.0
SFK574 (SFK574Q) /CSM/SF/SFK5-D/SFK574Q.Seq.d/                       1158   0.0
AHH295 (AHH295Q) /CSM/AH/AHH2-D/AHH295Q.Seq.d/                       1158   0.0
AFO194 (AFO194Q) /CSM/AF/AFO1-D/AFO194Q.Seq.d/                       1158   0.0
AFK396 (AFK396Q) /CSM/AF/AFK3-D/AFK396Q.Seq.d/                       1158   0.0
AFE226 (AFE226Q) /CSM/AF/AFE2-B/AFE226Q.Seq.d/                       1158   0.0
AFC763 (AFC763Q) /CSM/AF/AFC7-C/AFC763Q.Seq.d/                       1158   0.0
AFO753 (AFO753Q) /CSM/AF/AFO7-C/AFO753Q.Seq.d/                       1156   0.0
AFI737 (AFI737Q) /CSM/AF/AFI7-B/AFI737Q.Seq.d/                       1156   0.0
AFK395 (AFK395Q) /CSM/AF/AFK3-D/AFK395Q.Seq.d/                       1154   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.  1158   0.0    2
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.   961   0.0    4
BJ171907|BJ171907.1 Physcomitrella patens subsp. patens cDNA clone:pph30i20, 3' end, single read.   595   0.0    2
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.    88   2e-56  7
L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.    70   1e-50  7
AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    88   6e-41  8
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.    82   8e-18  4
AF426414|AF426414.1 Delia radicum cathepsin L-like cysteine protease precursor (CP1) mRNA, complete cds.    56   1e-15  4
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.    46   2e-13  5
BQ834906|BQ834906.1 Po_ad_04H08_TEXF1 Psoroptes ovis mixed Psoroptes ovis cDNA clone Po_ad_04H08 5' similar to O46030 CYSTEINE PROTEINASE. Sitophilus zeamais (maize weevil), mRNA sequence.    50   4e-06  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...   379   e-105
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...   202   1e-51
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...   202   1e-51
BC075887_1(BC075887|pid:none) Danio rerio cathepsin L.1, mRNA (c...   189   6e-47
AY220615_1(AY220615|pid:none) Hydra vulgaris cathepsin L precurs...   187   3e-46
DQ280314_1(DQ280314|pid:none) Hymeniacidon perlevis cathepsin L ...   186   4e-46
AY554271_1(AY554271|pid:none) Heterodera glycines cathepsin L-li...   183   3e-45
DQ474246_1(DQ474246|pid:none) Lygus lineolaris cathepsin-L mRNA,...   181   3e-45
L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   179   8e-45
AY336798_1(AY336798|pid:none) Rhipicephalus haemaphysaloides hae...   179   6e-44

 psg: 0.66  gvh: 0.35  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for AFI111 is cyt

>AFI111Z.Seq
----------CTCTACTACTGGTAGTACTGAAGGTGCTCATGCATTAAAAACTAAAAAAA
TTGGTTTCACTATCTGAACAAAATTTAGTTGATTGCTCTGGTCCAGAAGAAAACTTTGGT
TGTGATGGTGGTTTAATGAATAATGCATTTGATTATATCATTAAAAACAAGGGTATTGAC
ACTGAAAGTAGTTATCCATATACTGCTGAAACTGGTTCAACATGTTTATTCAATAAATCT
GATATTGGTGCAACCATTAAAGGTTATGTTAACATTACTGCCGGTTCAGAGATTTCATTA
GAGAATGGTGCTCAACATGGTCCAGTATCTGTTGCAATTGATGCTTCCCATAATAGTTTC
CAACTCTATACATCTGGTATCTATTATGAGCCAAAATGTTCTCCAACTGAATTGGATCAT
GGTGTTTTAGTTGTTGGTTATGGTGTTCAAGGTAAAGATGATGAAGGTCCAGTATTAAAC
AGAAAACAAACCATTGTTATCCACAAGAATGAAGATAATAAAGTTGAATCCTCTGATGAT
TCATCTGATAGTGTTAGACCAAAGGCAAATAATTATTGGATCGTTAAAAATTCATGGGGT
ACCTCATGGGGTATAAAAGGTTACATTTTAATGTCAAAGGATAGNACANNANAATTGTGG
TANCGCATCAGTT