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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFD205
Library SF
(Link to library)
Clone ID SFD205
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16451-1
Original site URL
Representative seq. ID SFD205P
(Link to Original site)
Representative DNA sequence 
>SFD205 (SFD205Q) /CSM/SF/SFD2-A/SFD205Q.Seq.d/
AACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAA
ACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAA
AAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCAC
AATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATC
GTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGATTAATGATGCCAATCAT
TTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCCA
AGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATTXXXX
XXXXXXTCACAAAAGAACCAATACGCTCGTGAGGATTACACCTACCAATTGAAATCATTA
TTAAAGGTTGTCCGTGACGTTAAGCCAACCGCTATCATTGGTTTATCAGGTATCGGTGGT
TCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAACACGTCGAGAAACCAATCGTTTTC
GCTCTCTCAAATCCAACCACCAACGCTGAATGTACCGCAGAACAAGCTTACCAATGGACC
GATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCAAACCAGTCGAATACAAAGGTAAA
ACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCTTCCCAGGTCTTGGTTTAGCCGCT
AGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGCCGCTAAAACTCTT
GCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCCAGGTCTCCAACAT
ATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGCTTATGAAGAAGGT
ATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATCTCGTCAATATGTT
CCATCTTATGATAAATCAAAAAATTGACTATCTTGCCCAATTTCACCAAATACAAATAGA
TTAAGTCCAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
trpnk*kkqnak*tiihfkkpis**rywf***rkrkirikrfittkg*iitrtir*siit
iyii*yklgkiyflklft*s**nfillfik**FRLMMPIIYTPTVGEACQKFGNEFRFAQ
GMYFASQDKGNIRAMMDN---

---SQKNQYAREDYTYQLKSLLKVVRDVKPTAIIGLSGIGGSFSQEVIEEMAKHVEKPIV
FALSNPTTNAECTAEQAYQWTDGRCIFASGSPFKPVEYKGKTFVPGQGNNMYIFPGLGLA
ASVCEAKHVTDAMIITAAKTLASFVEDSEVLTGKIYPGLQHIREISTRIAVKVIEKAYEE
GMAQLPRPDNIEALVKSRQYVPSYDKSKN*lscpispntnrlsp
Translated Amino Acid sequence (All Frames) 
Frame A:
nst*qikkakckinhhsf*ethqlikvlvlimkkekn*d*kvyyhqrlnhyknnqiehyh
nlhhliqtwkdifs*ivyvivmklysiiy*vii*indanhlytnrr*smskvw**ipfrp
ryvfcipr*r*hschdgq---

---SQKNQYAREDYTYQLKSLLKVVRDVKPTAIIGLSGIGGSFSQEVIEEMAKHVEKPIV
FALSNPTTNAECTAEQAYQWTDGRCIFASGSPFKPVEYKGKTFVPGQGNNMYIFPGLGLA
ASVCEAKHVTDAMIITAAKTLASFVEDSEVLTGKIYPGLQHIREISTRIAVKVIEKAYEE
GMAQLPRPDNIEALVKSRQYVPSYDKSKN*lscpispntnrlsp

Frame B:
trpnk*kkqnak*tiihfkkpis**rywf***rkrkirikrfittkg*iitrtir*siit
iyii*yklgkiyflklft*s**nfillfik**FRLMMPIIYTPTVGEACQKFGNEFRFAQ
GMYFASQDKGNIRAMMDN---

---hkrtntlvritptn*nhy*rlsvtlsqplslvyqvsvvhslkk*lkkwpntsrnqsf
slsqiqpptlnvpqnkltngpmvvvslplvhhsnqsntkvkplfqvkvtictssqvlv*p
lafvklnmslml*sllplkllppl*ktlkfslvksiqvsnifvksqqesplkslkklmkk
vwlnyhvqiilkl*snlvnmfhlminqkidylaqfhqiqid*vq

Frame C:
ldltnkkskmqnkpsfilrnpsankgtgfnneereklglkgllppkveslqeqsdralsq
ftsfntnleryiflnclrdrnetlfyyllsnnld**cqsfihqp*vkhvkslvmnsvspk
vcilhpkikvtfvp*wti---

---tkepirs*glhlpieiiikgcp*r*anryhwfiryrwfilsrsn*rngqtrretnrf
rslksnhqr*myrrtslpmdrwslylclwftiqtsriqr*nlcsrsr*qyvhlprswfsr
*rl*s*tch*cydhycr*nscllcrrl*sshw*nlsrsptys*nlnknrr*sh*ksl*rr
ygsittsr*y*sfsqissicsil**ikkltilpnftkyk*iks
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFD205 (SFD205Q) /CSM/SF/SFD2-A/SFD205Q.Seq.d/                       2060   0.0
SLB437 (SLB437Q) /CSM/SL/SLB4-B/SLB437Q.Seq.d/                       1300   0.0
CHB626 (CHB626Q) /CSM/CH/CHB6-B/CHB626Q.Seq.d/                       1298   0.0
CFJ334 (CFJ334Q) /CSM/CF/CFJ3-B/CFJ334Q.Seq.d/                       1285   0.0
VFK276 (VFK276Q) /CSM/VF/VFK2-D/VFK276Q.Seq.d/                       1277   0.0
VFI106 (VFI106Q) /CSM/VF/VFI1-A/VFI106Q.Seq.d/                       1219   0.0
CFG469 (CFG469Q) /CSM/CF/CFG4-C/CFG469Q.Seq.d/                       1215   0.0
CFH782 (CFH782Q) /CSM/CF/CFH7-D/CFH782Q.Seq.d/                       1209   0.0
CHA161 (CHA161Q) /CSM/CH/CHA1-C/CHA161Q.Seq.d/                       1193   0.0
CHA431 (CHA431Q) /CSM/CH/CHA4-B/CHA431Q.Seq.d/                       1170   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY387644|AY387644.1 Dictyostelium discoideum malic enzyme gene, complete cds.  1300   0.0    4
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.  1300   0.0    7
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   3e-04  16
AE017029|AE017029.1 Bacillus anthracis str. Ames section 6 of 18 of the complete genome.    52   0.016  1
Y09212|Y09212.1 B.cereus partial gltT, aspA and partial maoX genes.    52   0.016  1
AY194854|AY194854.1 Meloidogyne mayaguensis 5S-18S ribosomal RNA intergenic spacer, partial sequence.    40   0.037  2
BP506190|BP506190.1 Hydra magnipapillata cDNA, clone:hm_04308.     42   0.054  2
BQ120661|BQ120661.2 EST606237 mixed potato tissues Solanum tuberosum cDNA clone STMES17 5' end, mRNA sequence.    44   0.064  2
CK272664|CK272664.1 EST718742 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAD658 3' end, mRNA sequence.    44   0.066  2
AL929356|AL929356.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 2/5.    34   0.090  12
dna update 2003.12.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001322_605(CP001322|pid:none) Desulfatibacillum alkenivorans A...   188   3e-46
AY149910_1(AY149910|pid:none) Mastigamoeba balamuthi malic enzym...   187   7e-46
AY594688_1(AY594688|pid:none) Hydrilla verticillata NADP-depende...   187   7e-46
(P34105) RecName: Full=NADP-dependent malic enzyme;          Sho...   186   1e-45
CP001032_3280(CP001032|pid:none) Opitutus terrae PB90-1, complet...   186   2e-45
EU118588_1(EU118588|pid:none) Ovis aries cytosolic NADP+-depende...   185   2e-45
AB078329_1(AB078329|pid:none) Lithospermum erythrorhizon LeME mR...   185   2e-45
FJ495084_1(FJ495084|pid:none) Bos taurus malic enzyme 1 (ME1) mR...   184   6e-45
AC010793_18(AC010793|pid:none) Genomic sequence for Arabidopsis ...   183   8e-45
(Q29558) RecName: Full=NADP-dependent malic enzyme;          Sho...   183   8e-45
protein update 2009. 5.23
PSORT 

 psg: 0.92  gvh: 0.37  alm: 0.39  top: 0.40  tms: 0.00  mit: 0.24  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for SFD205 is nuc
5' end seq. ID SFD205F
5' end seq. 
>SFD205F.Seq
AACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAA
ACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAA
AAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCAC
AATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATC
GTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGATTAATGATGCCAATCAT
TTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCCA
AGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATT----
------
Length of 5' end seq. 416
3' end seq. ID SFD205Z
3' end seq. 
>SFD205Z.Seq
----------TCACAAAAGAACCAATACGCTCGTGAGGATTACACCTACCAATTGAAATC
ATTATTAAAGGTTGTCCGTGACGTTAAGCCAACCGCTATCATTGGTTTATCAGGTATCGG
TGGTTCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAACACGTCGAGAAACCAATCGT
TTTCGCTCTCTCAAATCCAACCACCAACGCTGAATGTACCGCAGAACAAGCTTACCAATG
GACCGATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCAAACCAGTCGAATACAAAGG
TAAAACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCTTCCCAGGTCTTGGTTTAGC
CGCTAGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGCCGCTAAAAC
TCTTGCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCCAGGTCTCCA
ACATATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGCTTATGAAGA
AGGTATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATCTCGTCAATA
TGTTCCATCTTATGATAAATCAAAAAATTGACTATCTTGCCCAATTTCACCAAATACAAA
TAGATTAAGTCCAA
Length of 3' end seq. 664
Connected seq. ID SFD205P
Connected seq. 
>SFD205P.Seq
AACTCGACCTAACAAATAAAAAAAGCAAAATGCAAAATAAACCATCATTCATTTTAAGAA
ACCCATCAGCTAATAAAGGTACTGGTTTTAATAATGAAGAAAGAGAAAAATTAGGATTAA
AAGGTTTATTACCACCAAAGGTTGAATCATTACAAGAACAATCAGATAGAGCATTATCAC
AATTTACATCATTTAATACAAACTTGGAAAGATATATTTTCTTAAATTGTTTACGTGATC
GTAATGAAACTTTATTCTATTATTTATTAAGTAATAATTTAGATTAATGATGCCAATCAT
TTATACACCAACCGTAGGTGAAGCATGTCAAAAGTTTGGTAATGAATTCCGTTTCGCCCA
AGGTATGTATTTTGCATCCCAAGATAAAGGTAACATTCGTGCCATGATGGACAATT----
------TCACAAAAGAACCAATACGCTCGTGAGGATTACACCTACCAATTGAAATCATTA
TTAAAGGTTGTCCGTGACGTTAAGCCAACCGCTATCATTGGTTTATCAGGTATCGGTGGT
TCATTCTCTCAAGAAGTAATTGAAGAAATGGCCAAACACGTCGAGAAACCAATCGTTTTC
GCTCTCTCAAATCCAACCACCAACGCTGAATGTACCGCAGAACAAGCTTACCAATGGACC
GATGGTCGTTGTATCTTTGCCTCTGGTTCACCATTCAAACCAGTCGAATACAAAGGTAAA
ACCTTTGTTCCAGGTCAAGGTAACAATATGTACATCTTCCCAGGTCTTGGTTTAGCCGCT
AGCGTTTGTGAAGCTAAACATGTCACTGATGCTATGATCATTACTGCCGCTAAAACTCTT
GCCTCCTTTGTAGAAGACTCTGAAGTTCTCACTGGTAAAATCTATCCAGGTCTCCAACAT
ATTCGTGAAATCTCAACAAGAATCGCCGTTAAAGTCATTGAAAAAGCTTATGAAGAAGGT
ATGGCTCAATTACCACGTCCAGATAATATTGAAGCTTTAGTCAAATCTCGTCAATATGTT
CCATCTTATGATAAATCAAAAAATTGACTATCTTGCCCAATTTCACCAAATACAAATAGA
TTAAGTCCAA
Length of connected seq. 1080
Full length Seq ID -
Full length Seq. -
Length of full length seq. -