>SFD132 (SFD132Q) /CSM/SF/SFD1-B/SFD132Q.Seq.d/
XXXXXXXXXXATTCTATGCACCATGGTGTGGTCATTGCAAAAAAAGTTAATGCCAGATTA
TGAAATCCTTGGTAACACCTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTG
CGATGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACT
CAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGA
TACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACT
CGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGC
CGCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCC
AGAAGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAA
ATCAATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAAT
GTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATC
AAAATAAATTAAATTTAATTTAGAAAATAAAAAATAAAATAAAAGAAAAAAAATAAAATA
TAAGGATANAATTTTAAA

---LCTMVWSLQKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLK
WFGKQSKDGEKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAA
AEVRKELVKKAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMS
GKKADEFAKKLNILESFKSK*ikfnlenkk*nkrkkikykdxil

Frame A:
---fyapwcghckks*cqimkslvtptpmkkmlsspkliamllitkpsapnmvslvsqhs
ngsvnkaktvknmnkvvililssttstnklvltvskvvnsllvlvvlnnsillplnsslp
llksvrnssrklkpslilsqkn*elkvhttsks*kpllknqsil*plkslvspn*slvqc
lerklmnlprnsif*nlsnqnklnli*kiknkikekk*nirixf*


Frame B:
---smhhgvviakkvnarl*npw*hlrq*krcchrqn*lrcc**qshllqiwchwfpntq
mvr*tkqrr*ki*trs*s*yfhqlhqqtswc*pcqrw*trcwcwsc*ttryych*ihrcr
c*sp*gtrqessnrr*fsprrikn*rfilrqshenhc*kinrfcnh*nrsyhqislwfnv
wkes**icqetqyfrifqikin*i*frk*kik*kkknki*gxnfk


Frame C:
---LCTMVWSLQKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLK
WFGKQSKDGEKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAA
AEVRKELVKKAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMS
GKKADEFAKKLNILESFKSK*ikfnlenkk*nkrkkikykdxil

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFD132 (SFD132Q) /CSM/SF/SFD1-B/SFD132Q.Seq.d/                       1116   0.0
VFO821 (VFO821Q) /CSM/VF/VFO8-A/VFO821Q.Seq.d/                       1106   0.0
VFO241 (VFO241Q) /CSM/VF/VFO2-B/VFO241Q.Seq.d/                       1106   0.0
VFN359 (VFN359Q) /CSM/VF/VFN3-C/VFN359Q.Seq.d/                       1106   0.0
VFN233 (VFN233Q) /CSM/VF/VFN2-B/VFN233Q.Seq.d/                       1106   0.0
VFN206 (VFN206Q) /CSM/VF/VFN2-A/VFN206Q.Seq.d/                       1106   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                       1106   0.0
VFM537 (VFM537Q) /CSM/VF/VFM5-B/VFM537Q.Seq.d/                       1106   0.0
VFM151 (VFM151Q) /CSM/VF/VFM1-C/VFM151Q.Seq.d/                       1106   0.0
VFL584 (VFL584Q) /CSM/VF/VFL5-D/VFL584Q.Seq.d/                       1106   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1088   0.0    2
AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  1080   0.0    2
BI317339|BI317339.1 saf70a03.y1 Gm-c1078 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1078-1541 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    56   6e-04  1
BG362767|BG362767.1 sac08f03.y1 Gm-c1040 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1040-4086 5' similar to SW:ERP5_MEDSA P38661 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    56   6e-04  1
AW620967|AW620967.1 sj98c05.y1 Gm-c1023 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1023-2625 5' similar to SW:ERP5_MEDSA P38661 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    56   6e-04  1
AW508381|AW508381.1 si40c04.y1 Gm-r1030 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-r1030-1375 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    56   6e-04  1
BM898120|BM898120.1 ph59g02.y1 Ostertagia ostertagi L4 pAMP1 v1 Ostertagia ostertagi cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    54   0.003  1
CF591582|CF591582.1 AGENCOURT_15680722 NICHD_XGC_Swb1N Silurana tropicalis cDNA clone IMAGE:7023074 5', mRNA sequence.    54   0.003  1
CF376429|CF376429.1 AGENCOURT_15340000 NICHD_XGC_Swb1N Silurana tropicalis cDNA clone IMAGE:7004527 5', mRNA sequence.    54   0.003  1
CF590092|CF590092.1 AGENCOURT_15681378 NICHD_XGC_Swb1N Silurana tropicalis cDNA clone IMAGE:7020764 5', mRNA sequence.    54   0.003  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   341   3e-96
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   339   1e-95
AE017341_67(AE017341|pid:none) Cryptococcus neoformans var. neof...   139   5e-34
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   132   3e-33
EU284948_1(EU284948|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   131   9e-33
(O22263) RecName: Full=Probable protein disulfide-isomerase A6; ...   128   7e-32
AK317714_1(AK317714|pid:none) Arabidopsis thaliana AT2G47470 mRN...   128   8e-32
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   127   1e-31
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   127   2e-31
AY739288_1(AY739288|pid:none) Zea mays protein disulfide isomera...   124   8e-31

 psg: 0.69  gvh: 0.33  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for SFD132 is nuc

>SFD132Z.Seq
----------ATTCTATGCACCATGGTGTGGTCATTGCAAAAAAAGTTAATGCCAGATTA
TGAAATCCTTGGTAACACCTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTG
CGATGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACT
CAAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGA
TACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACT
CGCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGC
CGCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCC
AGAAGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAA
ATCAATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAAT
GTCTGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATC
AAAATAAATTAAATTTAATTTAGAAAATAAAAAATAAAATAAAAGAAAAAAAATAAAATA
TAAGGATANAATTTTAAA