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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFD101
Library SF
(Link to library)
Clone ID SFD101
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFD101P
(Link to Original site)
Representative DNA sequence 
>SFD101 (SFD101Q) /CSM/SF/SFD1-A/SFD101Q.Seq.d/
TTGGCCTACTGGAAAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTT
ATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAAACCNCCTTTTT
TGAAAAAAAAAAXXXXXXXXXXANAATGTAAAACCAANNTTCAANATAAGGAAGGTTTCC
CCCAGATCAACAACGTTTCATTTTNGTTGNTAACCATTTANAANATGNTCGGACTCTCTC
TGATTCAATATCCAAAAAGAATCAGCTCTCCACTTGGTCCTCAGATTAAGAGNTGGTATG
CAAATCTTTGTCNAAACCATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGA
TAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACA
AAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACAT
TCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGT
CAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAA
TGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTT
CGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATC
CACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCAC
TGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAA
AATTCAAGACAAAGAAGGTATCCC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
gllensfllyky*ihilffyil*HFIKKKKKKKXKKPPFLKKK---

---XCKTXXQXKEGFPQINNVSFXLXTIXXMXGLSLIQYPKRISSPLGPQIKXWYANLCX
NH*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslittfkrnp
lsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspvnn
wrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfktk
kvs
Translated Amino Acid sequence (All Frames) 
Frame A:
laywkihfyytnikfifyffiycnis*kkkkkkxlknxlf*kkk---

---xm*nqxsx*grfppdqqrfixvxnhlxxxrtlsdsiskknqlstwssd*exvcksls
kpltgktitlevegsdnienvktkiqdkegippdqqrlifagkqledgrtlsdyniqkes
tlhlvlrlrggmqifvktltgktitlevegsdnienvkakiqdkegippdqqrlifagkq
ledgrtlsdyniqkestlhlvlrlrggmqifvktltgktitlevegsdnienvktkiqdk
egi

Frame B:
wptgkfifiiqilnsyfiflyivtfhkkkkkkkx*ktxffekk---

---XCKTXXQXKEGFPQINNVSFXLXTIXXMXGLSLIQYPKRISSPLGPQIKXWYANLCX
NH*lvrpsllklraqiilkmlkqrfktkkvshqtnkd*fslvnn*kmvvlslittfkrnp
lsi*ssd*evvckflskpslvrllhsklkvlitlrmlkpksktkkvfhqinnvsfspvnn
wrmvvlslittfkrnplsi*fsd*evvckfl*klslvkplhsklkvvttlrm*kqkfktk
kvs

Frame C:
gllensfllyky*ihilffyil*HFIKKKKKKKXKKPPFLKKK---

---nvkpxfxirkvsprsttfhfxx*pfxxxsdsl*fniqkesalhlvlrlrxgmqifvx
tinw*dhhs*s*glr*y*kc*nkdsrqrryptrptkinfrw*tirrwsysl*lqhskgih
spfspqikrwyanfcqnphw*dyytrs*rf**h*ec*sqnprqrrystrsttshfrr*ti
ggwsysl*lqhskgihspfssqikrwyanfcknshw*nhytrs*r**qh*ecknknsrqr
ryp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFD101 (SFD101Q) /CSM/SF/SFD1-A/SFD101Q.Seq.d/                       1368   0.0
SFJ493 (SFJ493Q) /CSM/SF/SFJ4-D/SFJ493Q.Seq.d/                       1277   0.0
SFJ221 (SFJ221Q) /CSM/SF/SFJ2-A/SFJ221Q.Seq.d/                       1273   0.0
CHE361 (CHE361Q) /CSM/CH/CHE3-C/CHE361Q.Seq.d/                       1273   0.0
CHC172 (CHC172Q) /CSM/CH/CHC1-C/CHC172Q.Seq.d/                       1273   0.0
CHE384 (CHE384Q) /CSM/CH/CHE3-D/CHE384Q.Seq.d/                       1267   0.0
CHC282 (CHC282Q) /CSM/CH/CHC2-D/CHC282Q.Seq.d/                       1267   0.0
CFJ702 (CFJ702Q) /CSM/CF/CFJ7-A/CFJ702Q.Seq.d/                       1267   0.0
CHD793 (CHD793Q) /CSM/CH/CHD7-D/CHD793Q.Seq.d/                       1265   0.0
CFC246 (CFC246Q) /CSM/CF/CFC2-B/CFC246Q.Seq.d/                       1265   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.  1076   0.0    4
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.  1061   0.0    4
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.  1061   0.0    4
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   638   0.0    4
M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   650   0.0    4
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   634   e-178  3
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   484   e-157  4
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   484   e-157  4
BJ541175|BJ541175.1 Oryzias latipes cDNA clone:MF01SSB034A18, 3' end single read.   176   1e-99  7
BJ528884|BJ528884.1 Oryzias latipes cDNA clone:MF01SSB034A18, 5' end single read.   192   2e-93  6
dna update 2003.12.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   369   e-101
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   369   e-101
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   369   e-101
M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   365   1e-99
A29584(A29584) ubiquitin precursor - pig (fragment)  &M18159_1(M...   335   2e-97
UQFS(S03599)ubiquitin precursor - common sunflower (fragment)         344   3e-96
T12035(T12035) polyubiquitin 4.4 - kidney bean  &U77939_1(U77939...   344   3e-96
AF528710_1(AF528710|pid:none) Arabidopsis thaliana ecotype Ita-0...   331   3e-96
AY957885_1(AY957885|pid:none) Gracilaria lemaneiformis polyubiqu...   353   4e-96
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   351   2e-95
protein update 2009. 5.23
PSORT 

 psg: 0.54  gvh: 0.46  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.61  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for SFD101 is nuc
5' end seq. ID SFD101F
5' end seq. 
>SFD101F.Seq
TTGGCCTACTGGAAAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTT
ATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAAACCNCCTTTTT
TGAAAAAAAAAA----------
Length of 5' end seq. 132
3' end seq. ID SFD101Z
3' end seq. 
>SFD101Z.Seq
----------ANAATGTAAAACCAANNTTCAANATAAGGAAGGTTTCCCCCAGATCAACA
ACGTTTCATTTTNGTTGNTAACCATTTANAANATGNTCGGACTCTCTCTGATTCAATATC
CAAAAAGAATCAGCTCTCCACTTGGTCCTCAGATTAAGAGNTGGTATGCAAATCTTTGTC
NAAACCATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGATAATATTGAAAA
TGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACAAAGATTAATTTT
CGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATC
CACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGTCAAAACCCTCAC
TGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAATGTTAAAGCCAA
AATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTTCGCCGGTAAACA
ATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATCCACTCTCCATTT
AGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCACTGGTAAAACCAT
TACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAAAATTCAAGACAA
AGAAGGTATCCC
Length of 3' end seq. 722
Connected seq. ID SFD101P
Connected seq. 
>SFD101P.Seq
TTGGCCTACTGGAAAATTCATTTTTATTATACAAATATTAAATTCATATTTTATTTTTTT
ATATATTGTAACATTTCATAAAAAAAAAAAAAAAAAAAAAANTTAAAAAACCNCCTTTTT
TGAAAAAAAAAA----------ANAATGTAAAACCAANNTTCAANATAAGGAAGGTTTCC
CCCAGATCAACAACGTTTCATTTTNGTTGNTAACCATTTANAANATGNTCGGACTCTCTC
TGATTCAATATCCAAAAAGAATCAGCTCTCCACTTGGTCCTCAGATTAAGAGNTGGTATG
CAAATCTTTGTCNAAACCATTAACTGGTAAGACCATCACTCTTGAAGTTGAGGGCTCAGA
TAATATTGAAAATGTTAAAACAAAGATTCAAGACAAAGAAGGTATCCCACCAGACCAACA
AAGATTAATTTTCGCTGGTAAACAATTAGAAGATGGTCGTACTCTCTCTGATTACAACAT
TCAAAAGGAATCCACTCTCCATTTAGTCCTCAGATTAAGAGGTGGTATGCAAATTTTTGT
CAAAACCCTCACTGGTAAGACTATTACACTCGAAGTTGAAGGTTCTGATAACATTGAGAA
TGTTAAAGCCAAAATCCAAGACAAAGAAGGTATTCCACCAGATCAACAACGTCTCATTTT
CGCCGGTAAACAATTGGAGGATGGTCGTACTCTCTCTGATTACAACATTCAAAAGGAATC
CACTCTCCATTTAGTTCTCAGATTAAGAGGTGGTATGCAAATTTTTGTAAAAACTCTCAC
TGGTAAAACCATTACACTCGAAGTTGAAGGTAGTGACAACATTGAGAATGTAAAAACAAA
AATTCAAGACAAAGAAGGTATCCC
Length of connected seq. 854
Full length Seq ID -
Full length Seq. -
Length of full length seq. -