>SFC877 (SFC877Q) /CSM/SF/SFC8-D/SFC877Q.Seq.d/
ATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAG
AGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGA
TGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGA
AGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGC
AATCAAGAACATTCTCGAAGTCATTCAACCAGCCGTXXXXXXXXXX

SLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYE
AVELRDGDKSRYLGKGVLKAIKNILEVIQPA---

Frame A:
isiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssircinwyl*
sc*ikrw**ikilr*rctqsnqehsrshsts---


Frame B:
SLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYE
AVELRDGDKSRYLGKGVLKAIKNILEVIQPA---


Frame C:
lylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhqlvfmk
lln*emvinqdt*vkvyskqsrtfsksfnqp---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC877 (SFC877Q) /CSM/SF/SFC8-D/SFC877Q.Seq.d/                        547   e-155
VFM227 (VFM227Q) /CSM/VF/VFM2-B/VFM227Q.Seq.d/                        539   e-153
VFL491 (VFL491Q) /CSM/VF/VFL4-D/VFL491Q.Seq.d/                        539   e-153
VFH402 (VFH402Q) /CSM/VF/VFH4-A/VFH402Q.Seq.d/                        539   e-153
VFH378 (VFH378Q) /CSM/VF/VFH3-D/VFH378Q.Seq.d/                        539   e-153
VFH161 (VFH161Q) /CSM/VF/VFH1-C/VFH161Q.Seq.d/                        539   e-153
VFF154 (VFF154Q) /CSM/VF/VFF1-C/VFF154Q.Seq.d/                        539   e-153
VFF123 (VFF123Q) /CSM/VF/VFF1-A/VFF123Q.Seq.d/                        539   e-153
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                        539   e-153
VFD552 (VFD552Q) /CSM/VF/VFD5-C/VFD552Q.Seq.d/                        539   e-153

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL438980|AL438980.1 T3 end of clone BD0AA001F06 of library BD0AA from strain CBS 94 of  Candida tropicalis.    86   1e-17  2
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    62   2e-17  3
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    62   2e-17  3
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    62   2e-17  3
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    62   2e-17  3
AU086396|AU086396.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn2452.    62   2e-17  3
AU086101|AU086101.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn1183.    62   2e-17  3
AU086284|AU086284.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn2244.    62   2e-17  3
AU087778|AU087778.1 Plasmodium falciparum 3D7 cDNA clone from Sugano malaria cDNA library: XPFn5807.    62   7e-17  3
BM396115|BM396115.1 5009-0-17-E04.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    72   6e-16  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   156   2e-37
CR940353_640(CR940353|pid:none) Theileria annulata strain Ankara...   119   4e-26
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   116   3e-25
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   114   1e-24
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   113   2e-24
CR932180_1(CR932180|pid:none) Paramecium tetraurelia, enolase, p...   112   5e-24
(Q967Y8) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   112   5e-24
CR932190_1(CR932190|pid:none) Paramecium tetraurelia, enolase, p...   111   7e-24
(A4ISP4) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   110   1e-23
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   110   1e-23

 psg: 0.71  gvh: 0.46  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal

>> prediction for SFC877 is nuc

>SFC877F.Seq
ATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAG
AGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGA
TGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGA
AGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGC
AATCAAGAACATTCTCGAAGTCATTCAACCAGCCGT----------