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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC750
Library SF
(Link to library)
Clone ID SFC750
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SFC750P
(Link to Original site)
Representative DNA sequence 
>SFC750 (SFC750Q) /CSM/SF/SFC7-C/SFC750Q.Seq.d/
CTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAA
ATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGT
GTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCT
GNTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGGACTCAAAGCAATC
AAAAACATTCTCGAXXXXXXXXXXACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTG
CCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACG
GTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAAT
ATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAAT
TAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAA
GAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAA
TTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTG
TCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTG
GTTTAGGTACNGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAAT
ACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTAAAACCATAACTATGCTGGTTNACT
TTCCGTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEA
XELRDGDKSRYLGKGGLKAIKNIL---

---TGLVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISI
EDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTE
SIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVR
INEELGKTITMLVXFP*
Translated Amino Acid sequence (All Frames) 
Frame A:
LSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEA
XELRDGDKSRYLGKGGLKAIKNIL---

---tqv*lklvwivlplnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfq
lkihlikmignhtln*qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqsl
nqfvplltqrmhhgvswlaivqvklkipslli*llv*vxvklklvlhvdqkd*pntinll
eltks*vkp*lcwxtfr

Frame B:
ylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhqlvfmkl
xn*emvinqdt*vkvdskqsktfs---

---hrfs*nwyglccl*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn
*rsi*sr*lgiih*insis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*
inscrs*lkecimgchg*psfr*n*rylhc*fscwfryxsn*nwcsm*irkisqiqstc*
n*rrvr*nhnyagxlsv

Frame C:
iyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssircinwyl*sx
*ikrw**ikilr*rwtqsnqkhsr---

---TGLVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISI
EDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTE
SIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVR
INEELGKTITMLVXFP*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC750 (SFC750Q) /CSM/SF/SFC7-C/SFC750Q.Seq.d/                       1604   0.0
AFO841 (AFO841Q) /CSM/AF/AFO8-B/AFO841Q.Seq.d/                       1134   0.0
AFB673 (AFB673Q) /CSM/AF/AFB6-D/AFB673Q.Seq.d/                       1130   0.0
VFL491 (VFL491Q) /CSM/VF/VFL4-D/VFL491Q.Seq.d/                       1118   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1118   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1118   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1118   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1118   0.0
SFC432 (SFC432Q) /CSM/SF/SFC4-B/SFC432Q.Seq.d/                       1118   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1118   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   3e-53  8
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-45  7
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-45  7
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   2e-45  7
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   2e-45  7
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   115   2e-38  4
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           60   2e-37  7
BI073826|BI073826.1 kt35e07.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:ENO_CAEEL Q27527 ENOLASE ;, mRNA sequence.    94   5e-37  3
BI504105|BI504105.1 BB170030B20E01 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170030B20E01 5', mRNA sequence.    82   2e-36  4
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66   2e-35  4
dna update 2003.12.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   365   e-99
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   285   1e-75
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   278   1e-73
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   278   2e-73
AF100985_1(AF100985|pid:none) Penaeus monodon phosphopyruvate hy...   278   2e-73
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   274   3e-72
(P56252) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   271   2e-71
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   270   4e-71
(Q43130) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   269   7e-71
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   269   7e-71
protein update 2009. 5.22
PSORT 

 psg: 0.71  gvh: 0.46  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for SFC750 is nuc
5' end seq. ID SFC750F
5' end seq. 
>SFC750F.Seq
CTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAA
ATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGT
GTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCT
GNTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGGACTCAAAGCAATC
AAAAACATTCTCGA----------
Length of 5' end seq. 254
3' end seq. ID SFC750Z
3' end seq. 
>SFC750Z.Seq
----------ACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAA
GTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATC
TCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCA
ATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTT
GATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGT
ATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACT
GAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCAT
CGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACNGGT
CAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTT
AGAATTAACGAAGAGTTAGGTAAAACCATAACTATGCTGGTTNACTTTCCGTAA
Length of 3' end seq. 584
Connected seq. ID SFC750P
Connected seq. 
>SFC750P.Seq
CTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAA
ATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGT
GTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCT
GNTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAAAGGTGGACTCAAAGCAATC
AAAAACATTCTCGA----------ACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTG
CCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACG
GTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAAT
ATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAAT
TAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAA
GAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAA
TTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTG
TCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTG
GTTTAGGTACNGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAAT
ACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTAAAACCATAACTATGCTGGTTNACT
TTCCGTAA
Length of connected seq. 838
Full length Seq ID -
Full length Seq. -
Length of full length seq. -