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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC663
Library SF
(Link to library)
Clone ID SFC663
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14973-1
Original site URL
Representative seq. ID SFC663P
(Link to Original site)
Representative DNA sequence 
>SFC663 (SFC663Q) /CSM/SF/SFC6-C/SFC663Q.Seq.d/
ACATTCTTTCCAAACCAAAATAAAAAATGTTATCAAATTTTTTAAAAGTCAACAGTAAAG
CACTAGGACATATAAGAACTTTTGCCTCAAAGAGTGGTGAAATTAAACACAATTTTAAAA
AAGCCGATACATACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAAC
TCATTTCATTCTTCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGTT
TATACAAAAAAAAATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAANAAGCTGTTT
GTGCAGGTTTAGAAAGNGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGATC
ACACTTATATGTTATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATGA
AAGAAACTGGTTGTTCAAAGGGTAAAGGNGGTTCAATGCATATGTTTACAAANAATTTCT
ATGGTGGXXXXXXXXXXCGGTAATGTTTGTTTAGCCATGTATGGTGATGGTGCTGCCAAT
CAAGGTCAATTATTTGAAGCCTTCAACATGGCTTCACTCTGGAAACTTCCAGTCATTTTC
ATTTGTGAAAACAATAAATACGGTATGGGTACCTCTCAAAANAGATCAACTGCTGGTCAC
GATTTCTATACTCGTGGTCACTATGTTGCAGGCTTAAAAGTCGATGGTATGGATGTTTTC
GCCGTCAAANAAGCTGGTAAATACGCCGCTGAATGGTGTANAGCTGGTAATGGTCCAATC
ATTTTANAAATGGATACCTATCGTTATGTAGGTCACTCTATGTCTGATCCAGGTATCACT
TATCGTACAANAGAAGAAGTTAACCACGTTAGACAAACTAGAGATCCAATTGAAAACATT
CGTCAAATCATCTTGGACAATAAAATCGCAACTGAAGATCAACTCGCTGCCATCGAAGAA
ACCGTTANAGATGAAATGGAAAAAGCCTCTGAAAAAGCTATCGCTGCTCCATTACCACAA
GCTCGTNAACTCTTTACCAACGTTTACCTCCAAGAAGTTCCAGTAAGAGGTGTNAATTTG
TTAACAGTTTCAAACCATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ilskpk*KMLSNFLKVNSKALGHIRTFASKSGEIKHNFKKADTYLCDGPSDSTVTNKDEL
ISFFTEMSRFRRLETVCDGLYKKKLIRGFCHLYTGQXAVCAGLEXAITKDDHIITAYRDH
TYMLSRGATPEEIFAELLMKETGCSKGKGGSMHMFTXNFYG---

---GNVCLAMYGDGAANQGQLFEAFNMASLWKLPVIFICENNKYGMGTSQXRSTAGHDFY
TRGHYVAGLKVDGMDVFAVKXAGKYAAEWCXAGNGPIILXMDTYRYVGHSMSDPGITYRT
XEEVNHVRQTRDPIENIRQIILDNKIATEDQLAAIEETVXDEMEKASEKAIAAPLPQARX
LFTNVYLQEVPVRGVNLLTVSNH
Translated Amino Acid sequence (All Frames) 
Frame A:
tffpnqnkkcyqif*kstvkh*di*ellpqrvvklntilkkpihtyvmvhltqllptkmn
sfhsslkcqdledsklfvmvytkkn*levsviytlvxklfvqv*kxqllkmiillqhtei
tlicyqeeqhqkkflqny**kklvvqrvkxvqciclqxismv---

---r*clfshvw*wccqsrsii*slqhgftletsshfhl*kq*irygylskxincwsrfl
yswslccrlksrwygcfrrqxsw*irr*mvxsw*wsnhfxngylslcrslyv*sryhlsy
xrrs*pr*tn*rsn*khssnhlgq*nrn*rstrchrrnrxr*ngksl*ksyrcsittssx
tlyqrlpprssskrcxfvnsfkp

Frame B:
hsfqtkiknvikffksqq*strtyknfclkew*n*tqf*ksryilm*wsi*lncyqqr*t
hfilh*nvki*ktrncl*wfiqkkin*rflsfihwsxsclcrfrkxny*r*syyysiqrs
hlyvikrsntrrnfcriinernwlfkg*rxfnayvykxflww---

---GNVCLAMYGDGAANQGQLFEAFNMASLWKLPVIFICENNKYGMGTSQXRSTAGHDFY
TRGHYVAGLKVDGMDVFAVKXAGKYAAEWCXAGNGPIILXMDTYRYVGHSMSDPGITYRT
XEEVNHVRQTRDPIENIRQIILDNKIATEDQLAAIEETVXDEMEKASEKAIAAPLPQARX
LFTNVYLQEVPVRGVNLLTVSNH

Frame C:
ilskpk*KMLSNFLKVNSKALGHIRTFASKSGEIKHNFKKADTYLCDGPSDSTVTNKDEL
ISFFTEMSRFRRLETVCDGLYKKKLIRGFCHLYTGQXAVCAGLEXAITKDDHIITAYRDH
TYMLSRGATPEEIFAELLMKETGCSKGKGGSMHMFTXNFYG---

---vmfv*pcmvmvlpikvnylkpstwlhsgnfqsfsfvktintvwvplkxdqllvtisi
lvvtmlqa*ksmvwmfspsxklvntplngvxlvmvqsfxkwipivm*vtlcliqvslivq
xkklttldkleiqlktfvksswtiksqlkinslpskkplxmkwkkplkklsllhyhklvn
slptftskkfq*evxic*qfqti
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC663 (SFC663Q) /CSM/SF/SFC6-C/SFC663Q.Seq.d/                       1994   0.0
VFL810 (VFL810Q) /CSM/VF/VFL8-A/VFL810Q.Seq.d/                       1146   0.0
VFL294 (VFL294Q) /CSM/VF/VFL2-D/VFL294Q.Seq.d/                       1146   0.0
CFH686 (CFH686Q) /CSM/CF/CFH6-D/CFH686Q.Seq.d/                       1146   0.0
AFE319 (AFE319Q) /CSM/AF/AFE3-A/AFE319Q.Seq.d/                       1146   0.0
SFI644 (SFI644Q) /CSM/SF/SFI6-B/SFI644Q.Seq.d/                       1132   0.0
SFI495 (SFI495Q) /CSM/SF/SFI4-D/SFI495Q.Seq.d/                       1132   0.0
SFI357 (SFI357Q) /CSM/SF/SFI3-C/SFI357Q.Seq.d/                       1132   0.0
VFM465 (VFM465Q) /CSM/VF/VFM4-C/VFM465Q.Seq.d/                       1126   0.0
SFF218 (SFF218Q) /CSM/SF/SFF2-A/SFF218Q.Seq.d/                       1122   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CC819103|CC819103.1 1O0005I12R Oxytricha plasmid UUGC1O library Sterkiella histriomuscorum genomic clone UUGC1O0005I12 R, genomic survey sequence.    72   6e-19  4
BF096522|BF096522.1 EST360549 tomato nutrient deficient roots Lycopersicon esculentum cDNA clone cLEW12E7 5' sequence similar to pyruvate dehydrogenase E1 alpha subunit {Zea mays}, mRNA sequence.    64   4e-16  3
AF209924|AF209924.1 Lycopersicon esculentum pyruvate dehydrogenase mRNA, partial cds.    64   2e-15  3
AF023920|AF023920.1 Kluyveromyces lactis pyruvate dehydrogenase complex E1-alpha subunit (PDA1) gene, complete cds.    50   5e-15  4
AW906597|AW906597.1 EST342719 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA3D12, mRNA sequence.    64   2e-14  3
BM111840|BM111840.1 EST559376 potato roots Solanum tuberosum cDNA clone cPRO12F13 5' end, mRNA sequence.    64   4e-14  3
BQ045904|BQ045904.1 EST595022 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI13O13 5' end, mRNA sequence.    64   4e-14  3
CK257172|CK257172.1 EST740809 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCD333 5' end, mRNA sequence.    64   6e-14  3
CK255161|CK255161.1 EST738798 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCCJ14 5' end, mRNA sequence.    64   7e-14  3
Z26949|Z26949.1 S.tuberosum E1 alpha subunit of pyruvate dehydrogenase (lipoamide).    64   2e-13  3
dna update 2003.12.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C70) RecName: Full=Pyruvate dehydrogenase E1 component subun...   376   0.0
EU186336_1(EU186336|pid:none) Brassica rapa clone S66 pyruvate d...   217   3e-91
CR761539_1(CR761539|pid:none) Xenopus tropicalis finished cDNA, ...   213   7e-90
(Q8H1Y0) RecName: Full=Pyruvate dehydrogenase E1 component subun...   213   2e-89
CP000581_566(CP000581|pid:none) Ostreococcus lucimarinus CCE9901...   224   6e-89
BC106670_1(BC106670|pid:none) Xenopus laevis pyruvate dehydrogen...   214   7e-89
AL928875_10(AL928875|pid:none) Zebrafish DNA sequence from clone...   214   9e-89
DQ191640_1(DQ191640|pid:none) Solanum tuberosum unknown mRNA.         214   9e-89
BT059393_1(BT059393|pid:none) Salmo salar clone ssal-rgf-519-230...   213   1e-88
AF209924_1(AF209924|pid:none) Lycopersicon esculentum pyruvate d...   214   1e-88
protein update 2009. 5.22
PSORT 

 psg: 0.67  gvh: 0.42  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.04
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for SFC663 is nuc
5' end seq. ID SFC663F
5' end seq. 
>SFC663F.Seq
ACATTCTTTCCAAACCAAAATAAAAAATGTTATCAAATTTTTTAAAAGTCAACAGTAAAG
CACTAGGACATATAAGAACTTTTGCCTCAAAGAGTGGTGAAATTAAACACAATTTTAAAA
AAGCCGATACATACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAAC
TCATTTCATTCTTCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGTT
TATACAAAAAAAAATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAANAAGCTGTTT
GTGCAGGTTTAGAAAGNGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGATC
ACACTTATATGTTATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATGA
AAGAAACTGGTTGTTCAAAGGGTAAAGGNGGTTCAATGCATATGTTTACAAANAATTTCT
ATGGTGG----------
Length of 5' end seq. 487
3' end seq. ID SFC663Z
3' end seq. 
>SFC663Z.Seq
----------CGGTAATGTTTGTTTAGCCATGTATGGTGATGGTGCTGCCAATCAAGGTC
AATTATTTGAAGCCTTCAACATGGCTTCACTCTGGAAACTTCCAGTCATTTTCATTTGTG
AAAACAATAAATACGGTATGGGTACCTCTCAAAANAGATCAACTGCTGGTCACGATTTCT
ATACTCGTGGTCACTATGTTGCAGGCTTAAAAGTCGATGGTATGGATGTTTTCGCCGTCA
AANAAGCTGGTAAATACGCCGCTGAATGGTGTANAGCTGGTAATGGTCCAATCATTTTAN
AAATGGATACCTATCGTTATGTAGGTCACTCTATGTCTGATCCAGGTATCACTTATCGTA
CAANAGAAGAAGTTAACCACGTTAGACAAACTAGAGATCCAATTGAAAACATTCGTCAAA
TCATCTTGGACAATAAAATCGCAACTGAAGATCAACTCGCTGCCATCGAAGAAACCGTTA
NAGATGAAATGGAAAAAGCCTCTGAAAAAGCTATCGCTGCTCCATTACCACAAGCTCGTN
AACTCTTTACCAACGTTTACCTCCAAGAAGTTCCAGTAAGAGGTGTNAATTTGTTAACAG
TTTCAAACCATA
Length of 3' end seq. 602
Connected seq. ID SFC663P
Connected seq. 
>SFC663P.Seq
ACATTCTTTCCAAACCAAAATAAAAAATGTTATCAAATTTTTTAAAAGTCAACAGTAAAG
CACTAGGACATATAAGAACTTTTGCCTCAAAGAGTGGTGAAATTAAACACAATTTTAAAA
AAGCCGATACATACTTATGTGATGGTCCATCTGACTCAACTGTTACCAACAAAGATGAAC
TCATTTCATTCTTCACTGAAATGTCAAGATTTAGAAGACTCGAAACTGTTTGTGATGGTT
TATACAAAAAAAAATTAATTAGAGGTTTCTGTCATTTATACACTGGTCAANAAGCTGTTT
GTGCAGGTTTAGAAAGNGCAATTACTAAAGATGATCATATTATTACAGCATACAGAGATC
ACACTTATATGTTATCAAGAGGAGCAACACCAGAAGAAATTTTTGCAGAATTATTAATGA
AAGAAACTGGTTGTTCAAAGGGTAAAGGNGGTTCAATGCATATGTTTACAAANAATTTCT
ATGGTGG----------CGGTAATGTTTGTTTAGCCATGTATGGTGATGGTGCTGCCAAT
CAAGGTCAATTATTTGAAGCCTTCAACATGGCTTCACTCTGGAAACTTCCAGTCATTTTC
ATTTGTGAAAACAATAAATACGGTATGGGTACCTCTCAAAANAGATCAACTGCTGGTCAC
GATTTCTATACTCGTGGTCACTATGTTGCAGGCTTAAAAGTCGATGGTATGGATGTTTTC
GCCGTCAAANAAGCTGGTAAATACGCCGCTGAATGGTGTANAGCTGGTAATGGTCCAATC
ATTTTANAAATGGATACCTATCGTTATGTAGGTCACTCTATGTCTGATCCAGGTATCACT
TATCGTACAANAGAAGAAGTTAACCACGTTAGACAAACTAGAGATCCAATTGAAAACATT
CGTCAAATCATCTTGGACAATAAAATCGCAACTGAAGATCAACTCGCTGCCATCGAAGAA
ACCGTTANAGATGAAATGGAAAAAGCCTCTGAAAAAGCTATCGCTGCTCCATTACCACAA
GCTCGTNAACTCTTTACCAACGTTTACCTCCAAGAAGTTCCAGTAAGAGGTGTNAATTTG
TTAACAGTTTCAAACCATA
Length of connected seq. 1089
Full length Seq ID -
Full length Seq. -
Length of full length seq. -