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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC512
Library SF
(Link to library)
Clone ID SFC512
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID SFC512P
(Link to Original site)
Representative DNA sequence 
>SFC512 (SFC512Q) /CSM/SF/SFC5-A/SFC512Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGNAGAGTTCACCGTCATTCTCAACTACAAAAGGAAAGAACCAXXXXXXXXXX
AAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCA
ATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGG
GAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCA
AATCCAGAATACAAAGGTGAATGGGNACNCCCATTAATTGATAACCCAGAATACGCTGAA
GACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTT
AAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGAT
TTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTC
GAAGCCGCCAAAGCTGAANAGANAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCT
GCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTA
GTTAAAACTGANGNCAAAAAAGAAGAAGTCNAAAAATCNACCNAAAAAGT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKXSSPSFSTTKGKN---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWXXPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEE
AKDFXEKTFVANQEAEKKMFDDLEAAKAEXXKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTXXKKEEVXKSTXK
Translated Amino Acid sequence (All Frames) 
Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKXSSPSFSTTKGKN---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWXXPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEE
AKDFXEKTFVANQEAEKKMFDDLEAAKAEXXKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTXXKKEEVXKSTXK

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqxrvhrhsqlqkert---

---rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqr
ksqiqntkvngxxh*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskk
lkisxkrpllptkkpkrkclttskppklxrxrkltkn*lpkkllkkkpkklmkkkkkslk
ki*lklxxkkkksknxpkk

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkxeftvilnykrkep---

---gnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meske
npksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrxrlcrrs
*rfl*kdlccqprsrkenv*rprsrqs*xxkes*rkiscrksc*krsrrs**rrrrsr*r
rfs*n*xqkrrsxkixxks
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        860   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        860   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        860   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        860   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        860   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        860   0.0
VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/                        860   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        860   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        860   0.0
VFD449 (VFD449Q) /CSM/VF/VFD4-C/VFD449Q.Seq.d/                        860   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   860   0.0    6
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   2e-22  2
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    72   5e-15  3
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   1e-10  2
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   5e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74   5e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74   5e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   5e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74   5e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   5e-09  1
dna update 2003.12.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   448   e-124
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   287   5e-76
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   283   6e-75
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   283   6e-75
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   281   4e-74
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   281   4e-74
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   279   1e-73
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   277   5e-73
L27348_1(L27348|pid:none) Hordeum vulgare calreticulin (CRH1) mR...   276   7e-73
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   275   3e-72
protein update 2009. 5.22
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum

>> prediction for SFC512 is exc
5' end seq. ID SFC512F
5' end seq. 
>SFC512F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGNAGAGTTCACCGTCATTCTCAACTACAAAAGGAAAGAACCA----------
Length of 5' end seq. 530
3' end seq. ID SFC512Z
3' end seq. 
>SFC512Z.Seq
----------AAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATAT
CCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGA
CGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAA
GAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACNCCCATTAATTGATAACCCAGA
ATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATT
ATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGA
AGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTT
TGACGACCTCGAAGCCGCCAAAGCTGAANAGANAAAGAAAGCTGACGAAAAATTAGCTGC
CGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGA
AGAAGATTTAGTTAAAACTGANGNCAAAAAAGAAGAAGTCNAAAAATCNACCNAAAAAGT
Length of 3' end seq. 590
Connected seq. ID SFC512P
Connected seq. 
>SFC512P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGNAGAGTTCACCGTCATTCTCAACTACAAAAGGAAAGAACCA----------
AAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCA
ATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGG
GAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCA
AATCCAGAATACAAAGGTGAATGGGNACNCCCATTAATTGATAACCCAGAATACGCTGAA
GACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTT
AAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGAT
TTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTC
GAAGCCGCCAAAGCTGAANAGANAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCT
GCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTA
GTTAAAACTGANGNCAAAAAAGAAGAAGTCNAAAAATCNACCNAAAAAGT
Length of connected seq. 1120
Full length Seq ID -
Full length Seq. -
Length of full length seq. -