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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC271
Library SF
(Link to library)
Clone ID SFC271
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16328-1
Original site URL
Representative seq. ID SFC271P
(Link to Original site)
Representative DNA sequence 
>SFC271 (SFC271Q) /CSM/SF/SFC2-C/SFC271Q.Seq.d/
CACTGTTGGCCTACTGGNAATAATATAAAAAATGAATAAATTTTATTCATTACCATTAAT
TATTATTTTAATAATTTCGTGTGGTGCTTTTTTTATCCACGGAGAAGAGGCATATTTTGA
ATATAGGATTCATAATGAAAAATTTATTTTCAAACTTAGTGATGATATTAAAATTTCAAC
ATGCAAGAAGAGTATTAXXXXXXXXXXAGTATNTCCAGGTGATGTTATTCAAGATATTTT
ACGTGTTATTAATCGTGATGAAAAAGAAGTTTATGAAAGGTGATATTAATCAANCNTTAT
TACATCGTAATACCAAAAATCGTCAAGAGNAACCNGTANATGAAGGTGACNTTAANCAGN
ATTTATTACGTCGTAATACCAAAAATCNTCANGAAGNACAAGTATATCAAGGTGATATTA
TTCANGATATTTTACGTGTTTTAAATCATGNANAGAAAGNAGTTTACNAAGGTGATATTN
ANCANAATTTGTTGNGTCGANAAAATCATGATCATCCAGAAAAAGNGNTTTATNAAGGTG
ATATTAANCANNATTTATTACGTCGTAATACCAAAAACCNTCNANANAAACTTGTTNATG
AAGGTGACATTAANCANGATTTATTACGTCGTNATAACAAAAATCATANAGAAGNACNAG
TATATCAAGGTGATATTATTCANGATATTTTACGTGTTTTAAATCATGGAGAAAAAGAAG
TAAATNAGGATGAAATTGAAAAAGANTTGATGCNACGTANAAATCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllxii*KMNKFYSLPLIIILIISCGAFFIHGEEAYFEYRIHNEKFIFKLSDDIKIST
CKKSI---

---YXQVMLFKIFYVLLIVMKKKFMKGDINQXLLHRNTKNRQEXPVXEGDXXQXLLRRNT
KNXXEXQVYQGDIIXDILRVLNHXXKXVYXGDIXXNLLXRXNHDHPEKXXYXGDIXXXLL
RRNTKNXXXKLVXEGDIXXDLLRRXNKNHXEXXVYQGDIIXDILRVLNHGEKEVNXDEIE
KXLMXRXN
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgnnikne*ilfitinyyfnnfvwcffyprrrgif*i*ds**kiyfqt***y*nfn
mqeey---

---sxsr*cysryftcy*s**krsl*kvilixxyyivipkivkxnxxmkvtlxxiyyvvi
pkixxkxkyikvilfxifyvf*imxrkxftkvixxxicxvxkimiiqkkxfxkvilxxiy
yvvipktxxxnlxmkvtlxxiyyvvitkiixkxxyikvilfxifyvf*imekkk*xrmkl
kkx*cxvxi

Frame B:
tvgllxii*KMNKFYSLPLIIILIISCGAFFIHGEEAYFEYRIHNEKFIFKLSDDIKIST
CKKSI---

---vxpgdviqdilrvinrdekevyer*y*sxxits*yqkssrxtxx*r*x*xxfits*y
qkssxxtsisr*yysxyftcfksxxexslxr*yxxxfvxsxks*ssrkxxlxr*y*xxfi
ts*yqkpsxxtcx*r*h*xxfitsx*qksxrxtsisr*yysxyftcfkswrkrskxg*n*
krxdatxks

Frame C:
llaywx*ykk*infihyh*llf**frvvlflstekrhilnigfimknlfsnlvmilkfqh
arrvl---

---YXQVMLFKIFYVLLIVMKKKFMKGDINQXLLHRNTKNRQEXPVXEGDXXQXLLRRNT
KNXXEXQVYQGDIIXDILRVLNHXXKXVYXGDIXXNLLXRXNHDHPEKXXYXGDIXXXLL
RRNTKNXXXKLVXEGDIXXDLLRRXNKNHXEXXVYQGDIIXDILRVLNHGEKEVNXDEIE
KXLMXRXN
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC271 (SFC271Q) /CSM/SF/SFC2-C/SFC271Q.Seq.d/                       1150   0.0
CHM686 (CHM686Q) /CSM/CH/CHM6-D/CHM686Q.Seq.d/                        783   0.0
SHC775 (SHC775Q) /CSM/SH/SHC7-D/SHC775Q.Seq.d/                        777   0.0
SHL720 (SHL720Q) /CSM/SH/SHL7-A/SHL720Q.Seq.d/                        775   0.0
SHL717 (SHL717Q) /CSM/SH/SHL7-A/SHL717Q.Seq.d/                        775   0.0
SHL617 (SHL617Q) /CSM/SH/SHL6-A/SHL617Q.Seq.d/                        775   0.0
SHK754 (SHK754Q) /CSM/SH/SHK7-C/SHK754Q.Seq.d/                        775   0.0
SHJ802 (SHJ802Q) /CSM/SH/SHJ8-A/SHJ802Q.Seq.d/                        775   0.0
SHJ713 (SHJ713Q) /CSM/SH/SHJ7-A/SHJ713Q.Seq.d/                        775   0.0
SHG761 (SHG761Q) /CSM/SH/SHG7-C/SHG761Q.Seq.d/                        775   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M29237|M29237.1 D.discoideum cyclic AMP-inducible protein gene, complete cds.   519   0.0    8
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    32   4e-05  16
AP001118|AP001118.1 Buchnera aphidicola str. APS (Acyrthosiphon pisum) genomic DNA, complete sequence, segment 1/2.    34   4e-04  14
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    36   4e-04  12
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    34   6e-04  12
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    34   0.001  13
BX890634|BX890634.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    34   0.003  10
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    32   0.003  11
CG816720|CG816720.1 SOYDE19TV LargeInsertSoybeanGenLib Glycine max genomic clone H13H04:MTP10C13, genomic survey sequence.    34   0.003  4
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           38   0.008  21
dna update 2003.12.20
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3101297
Number of Hits to DB: 659,624,912
protein update 2009. 5.22
PSORT 

 psg: 0.98  gvh: 0.63  alm: 0.19  top: 0.27  tms: 0.13  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: plasma membrane
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SFC271 is end
5' end seq. ID SFC271F
5' end seq. 
>SFC271F.Seq
CACTGTTGGCCTACTGGNAATAATATAAAAAATGAATAAATTTTATTCATTACCATTAAT
TATTATTTTAATAATTTCGTGTGGTGCTTTTTTTATCCACGGAGAAGAGGCATATTTTGA
ATATAGGATTCATAATGAAAAATTTATTTTCAAACTTAGTGATGATATTAAAATTTCAAC
ATGCAAGAAGAGTATTA----------
Length of 5' end seq. 197
3' end seq. ID SFC271Z
3' end seq. 
>SFC271Z.Seq
----------AGTATNTCCAGGTGATGTTATTCAAGATATTTTACGTGTTATTAATCGTG
ATGAAAAAGAAGTTTATGAAAGGTGATATTAATCAANCNTTATTACATCGTAATACCAAA
AATCGTCAAGAGNAACCNGTANATGAAGGTGACNTTAANCAGNATTTATTACGTCGTAAT
ACCAAAAATCNTCANGAAGNACAAGTATATCAAGGTGATATTATTCANGATATTTTACGT
GTTTTAAATCATGNANAGAAAGNAGTTTACNAAGGTGATATTNANCANAATTTGTTGNGT
CGANAAAATCATGATCATCCAGAAAAAGNGNTTTATNAAGGTGATATTAANCANNATTTA
TTACGTCGTAATACCAAAAACCNTCNANANAAACTTGTTNATGAAGGTGACATTAANCAN
GATTTATTACGTCGTNATAACAAAAATCATANAGAAGNACNAGTATATCAAGGTGATATT
ATTCANGATATTTTACGTGTTTTAAATCATGGAGAAAAAGAAGTAAATNAGGATGAAATT
GAAAAAGANTTGATGCNACGTANAAATCA
Length of 3' end seq. 559
Connected seq. ID SFC271P
Connected seq. 
>SFC271P.Seq
CACTGTTGGCCTACTGGNAATAATATAAAAAATGAATAAATTTTATTCATTACCATTAAT
TATTATTTTAATAATTTCGTGTGGTGCTTTTTTTATCCACGGAGAAGAGGCATATTTTGA
ATATAGGATTCATAATGAAAAATTTATTTTCAAACTTAGTGATGATATTAAAATTTCAAC
ATGCAAGAAGAGTATTA----------AGTATNTCCAGGTGATGTTATTCAAGATATTTT
ACGTGTTATTAATCGTGATGAAAAAGAAGTTTATGAAAGGTGATATTAATCAANCNTTAT
TACATCGTAATACCAAAAATCGTCAAGAGNAACCNGTANATGAAGGTGACNTTAANCAGN
ATTTATTACGTCGTAATACCAAAAATCNTCANGAAGNACAAGTATATCAAGGTGATATTA
TTCANGATATTTTACGTGTTTTAAATCATGNANAGAAAGNAGTTTACNAAGGTGATATTN
ANCANAATTTGTTGNGTCGANAAAATCATGATCATCCAGAAAAAGNGNTTTATNAAGGTG
ATATTAANCANNATTTATTACGTCGTAATACCAAAAACCNTCNANANAAACTTGTTNATG
AAGGTGACATTAANCANGATTTATTACGTCGTNATAACAAAAATCATANAGAAGNACNAG
TATATCAAGGTGATATTATTCANGATATTTTACGTGTTTTAAATCATGGAGAAAAAGAAG
TAAATNAGGATGAAATTGAAAAAGANTTGATGCNACGTANAAATCA
Length of connected seq. 756
Full length Seq ID -
Full length Seq. -
Length of full length seq. -