>SFC246 (SFC246Q) /CSM/SF/SFC2-B/SFC246Q.Seq.d/
CACTGTTGGCCTACTGGTATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGA
TTGTGAAAGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACC
ATTTGTATCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTG
TTGTGATGATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTC
AAGTTGTAAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGA
ATCATTAGGTTGTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCACGTTA
TGGAACTTCTTGTTGCCCAAGAATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAA
ATGTGACAGAATGAGATGTNCAGAAGGATTCTATTGTGAAGAACAAXXXXXXXXXXTGGT
TATTTATGTAATCAAACCCGTTGTCCACACGATTATCAATGTGAAACCATTAGCACCAAT
ATCGTGAAATGTTCACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCTGGTTCC
AGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTT
TGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACT
TGTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCA
TCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACT
AGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCA
ACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACA
ACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCC
GATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGT
GAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCA
TCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCT
GCAAGCTCAGAAAGCTCAGAAAGCTCATCAGCCACTTCATAAATAAAAC

TVGLLVLYLSPTFGWGGGRDCESHRSEYTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNC
CDDLSCAKNTRTGEIFEIWSSCKPHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPRY
GTSCCPRIPRCVGHHGGGHKCDRMRCXEGFYCEEQ---

---GYLCNQTRCPHDYQCETISTNIVKCSPKNDECKWHRCPPGSSCFNSRNGPHCLANNV
FPQLCKVTQCPTDFSCKMIRGNPTCIKARPPVPPPHCSTCAELSSACNHVGMICIQVPSN
CTNTRFPCCPSHPICIHPSTTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSA
ASSADSSAASSSPSSSAASSAASSEPSSSAASSSAPSSASSSAPSSASSSAPSSSASSSA
ASSAASSESSESSSATS*ik

Frame A:
hcwptgiisitnfwlgwwqrl*kssfriym*ir*klcifticimlw*eriflc*s*s*kl
l**fimc*khenw*nl*dlvkl*tt*rfctisftkynhm*iirl*skrygm*mg*iittl
wnfllpknskmcwsswwrs*m*qnemxrrill*rt---

---wlfm*snplstrlsm*nh*hqyremftke*rm*maslstwfqllq**krstlscq*c
ipttl*sysmsn*fll*nd*r*snly*skttgttttllnlcraiism*srwndlysstkq
lyqy*ipmlpissnlyssinyccfnhcnncincrnynlcnycrynnwwnynwwfnf***c
cflsr**rcllitikqccfkcrfk*tik*rrfk*csilcfk*rsilclk*csiiisfkfs
ciiscklrklrklishfink

Frame B:
TVGLLVLYLSPTFGWGGGRDCESHRSEYTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNC
CDDLSCAKNTRTGEIFEIWSSCKPHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPRY
GTSCCPRIPRCVGHHGGGHKCDRMRCXEGFYCEEQ---

---GYLCNQTRCPHDYQCETISTNIVKCSPKNDECKWHRCPPGSSCFNSRNGPHCLANNV
FPQLCKVTQCPTDFSCKMIRGNPTCIKARPPVPPPHCSTCAELSSACNHVGMICIQVPSN
CTNTRFPCCPSHPICIHPSTTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSA
ASSADSSAASSSPSSSAASSAASSEPSSSAASSSAPSSASSSAPSSASSSAPSSSASSSA
ASSAASSESSESSSATS*ik

Frame C:
llaywyyiyhqllvgvvaeivkvivqnihvnqievvhiyhlyhvvvrknfsvliviieiv
vmiyhvlktrelvkslrfgqvvnhieilyhiihqiqphvnh*vvkqevwnvnglnhhhvm
ellvaqefqdvlvimvavinvte*dvqkdsivkn---

---viyvikpvvhtiinvkplapis*nvhqrmtnvngivvhlvpaasivetvhtvlpimy
shnfvkllnvqlislvk*leviqlvlkqdhryhhhiaqlvqsyhqhvitle*fvfkyqai
vpildshvahliqfvfihqllllqplqqlhqlsqlqplqllqvqqlvelqlvvqllivvl
lpqpivalpphhhqavllqvplqvnhqvaplqvvlhpllqvalhplpqvvlhhhqlqvql
hhqlqaqkaqkahqplhk*n

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC246 (SFC246Q) /CSM/SF/SFC2-B/SFC246Q.Seq.d/                       2411   0.0
SFK752 (SFK752Q) /CSM/SF/SFK7-C/SFK752Q.Seq.d/                       1524   0.0
SFD672 (SFD672Q) /CSM/SF/SFD6-C/SFD672Q.Seq.d/                       1524   0.0
SFF631 (SFF631Q) /CSM/SF/SFF6-B/SFF631Q.Seq.d/                       1522   0.0
SFD192 (SFD192Q) /CSM/SF/SFD1-D/SFD192Q.Seq.d/                       1522   0.0
CFI758 (CFI758Q) /CSM/CF/CFI7-C/CFI758Q.Seq.d/                       1522   0.0
CFI753 (CFI753Q) /CSM/CF/CFI7-C/CFI753Q.Seq.d/                       1522   0.0
CFF209 (CFF209Q) /CSM/CF/CFF2-A/CFF209Q.Seq.d/                       1522   0.0
AFL684 (AFL684Q) /CSM/AF/AFL6-D/AFL684Q.Seq.d/                       1522   0.0
SFI203 (SFI203Q) /CSM/SF/SFI2-A/SFI203Q.Seq.d/                       1520   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.  1524   0.0    6
BJ170760|BJ170760.1 Physcomitrella patens subsp. patens cDNA clone:pph26m16, 3' end, single read.   190   3e-44  1
AF279135|AF279135.1 Dictyostelium discoideum spore coat structural protein SP65 (cotE) gene, complete cds.    64   5e-06  1
BD099577|BD099577.1 Novel genes cloned in humanneuroblastoma and fragments thereof.    48   0.30   1
BD019639|BD019639.1 Novel gene and novel gene fragment cloned in human neuroblastoma.    48   0.30   1
AC134329|AC134329.1 Mus musculus chromosome UNK clone RP24-310D17, WORKING DRAFT SEQUENCE, 12 unordered pieces.    42   0.30   5
C26876|C26876.1 Oryza sativa (japonica cultivar-group) cDNA, partial sequence (C50317_1A).    36   0.71   2
BD100341|BD100341.1 Novel genes cloned in humanneuroblastoma and fragments thereof.    46   1.2    1
BD020403|BD020403.1 Novel gene and novel gene fragment cloned in human neuroblastoma.    46   1.2    1
BX284669|BX284669.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-280E18.    44   1.6    8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       344   4e-93
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   340   5e-92
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   133   1e-29
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    92   4e-17
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    69   3e-10
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    69   3e-10
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    64   1e-08
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    63   2e-08
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    58   6e-07
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    57   1e-06

 psg: 0.93  gvh: 0.77  alm: 0.45  top: 0.43  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for SFC246 is exc

>SFC246F.Seq
CACTGTTGGCCTACTGGTATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGA
TTGTGAAAGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACC
ATTTGTATCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTG
TTGTGATGATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTC
AAGTTGTAAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGA
ATCATTAGGTTGTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCACGTTA
TGGAACTTCTTGTTGCCCAAGAATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAA
ATGTGACAGAATGAGATGTNCAGAAGGATTCTATTGTGAAGAACAA----------

>SFC246Z.Seq
----------TGGTTATTTATGTAATCAAACCCGTTGTCCACACGATTATCAATGTGAAA
CCATTAGCACCAATATCGTGAAATGTTCACCAAAGAATGACGAATGTAAATGGCATCGTT
GTCCACCTGGTTCCAGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTTGCCAATAATG
TATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATGATTA
GAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCAACTT
GTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCA
ATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAA
CTACTGCTGCTTCAACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAA
CTACTGCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTG
CTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAA
GTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTT
CAAGTAGCGCTCCATCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAG
CTGCATCATCAGCTGCAAGCTCAGAAAGCTCAGAAAGCTCATCAGCCACTTCATAAATAA
AAC

>SFC246P.Seq
CACTGTTGGCCTACTGGTATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGA
TTGTGAAAGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACC
ATTTGTATCATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTG
TTGTGATGATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTC
AAGTTGTAAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGA
ATCATTAGGTTGTGAAGCAAGAGGTATGGAATGTGAATGGGTTGAATCATCACCACGTTA
TGGAACTTCTTGTTGCCCAAGAATTCCAAGATGTGTTGGTCATCATGGTGGCGGTCATAA
ATGTGACAGAATGAGATGTNCAGAAGGATTCTATTGTGAAGAACAA----------TGGT
TATTTATGTAATCAAACCCGTTGTCCACACGATTATCAATGTGAAACCATTAGCACCAAT
ATCGTGAAATGTTCACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCTGGTTCC
AGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTT
TGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACT
TGTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCA
TCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACT
AGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCA
ACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACA
ACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCC
GATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGT
GAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCA
TCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCT
GCAAGCTCAGAAAGCTCAGAAAGCTCATCAGCCACTTCATAAATAAAAC