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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC228
Library SF
(Link to library)
Clone ID SFC228
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U03237-1
Original site URL
Representative seq. ID SFC228P
(Link to Original site)
Representative DNA sequence 
>SFC228 (SFC228Q) /CSM/SF/SFC2-B/SFC228Q.Seq.d/
ATAAATATATCTACAAATAATAGTATTAAGAACTGATCAAAAAGAATATATTATTCTAAA
AATGAAAATTAAACTGTGTCAAATAAAAATGATGATTATACTTTTATTCATTATTTTTAT
TCAATTGGGAAATTGCCAACTAGAAAATAAAACAAATGCAACAACAACAACAACAACAAG
ATATTGTAATAATGACGAATCATGCAATGATGAAAATTTGTGTACAAATGAAATGTGTGA
TCCAGTTATTGGATGTATTTATGAAAATATTTCATGTGATGATGATAATGGATGTACAAA
AGATTTTTGTGATCCATTGACTGGATGCTTTCATAAGAGGGTGGTATGTGATGATAATGA
TAAATGTACTGATAATATATGTAATCCTGAGACTGGAACATGCTNATTTAGAAGAAGAAT
ATGTACTGATAATAATGATTGCACAATGGACTGXXXXXXXXXXAATATGTACTGATAATA
ATGATTGCACAATGGACTGGTGTAATCAATTGACAGGAGAATGTGTTTATCAACCGGTTT
TAGTTTGTGAAGATTATAATAGTTGTACAATTGATGTTTGTAATGAATACAAGGGTTGTC
AATTTTTTAATAGAACTTGTGATGATAGATGTGTTTGTACAATTGATACTTGTAAAAATT
CAACTGGTTGTATTCACACTCCAATATCATGTGACGACAATAATGATTGTACCATTGATA
ATTGTGATTGTAAACTTGGCTGTATTCATAAAGAATTGCCCGTTGATGATTTTAATAATT
GTACATTTGACCAATGTTCCCATGAAGAAGGTATAACTCACACTCCAATTTCCTGTGATG
ATAACAATGATTGTACAACTGATAGTTGTGATATTTCAATTGGTTGTATACATGATCAAA
TTCCTGATTGTACTATTAATACCTCATCTAATAATTCTCAAAATAATCCAAAAGATTGTA
TTGGTGAAAATTGTATAAATATTCCATTTTGTTCAATCAAAGATAAAAAGTGTTTAGAAT
TGGTTGAAGAAATAAAAAAAAATCAAACTTCTCAATTCCCTCCAACTTCTTCTTCCTCTT
CATCTCATTCTCAGATTTCAAAAAATAAAGAAATTATAATAATATCAACATTATTTTTAG
GTACTTTAACATNATCTCTAAATTTTAAGNATAATAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
*IYLQIIVLRTDQKEYIILKMKIKLCQIKMMIILLFIIFIQLGNCQLENKTNATTTTTTR
YCNNDESCNDENLCTNEMCDPVIGCIYENISCDDDNGCTKDFCDPLTGCFHKRVVCDDND
KCTDNICNPETGTCXFRRRICTDNNDCTMD---

---ICTDNNDCTMDWCNQLTGECVYQPVLVCEDYNSCTIDVCNEYKGCQFFNRTCDDRCV
CTIDTCKNSTGCIHTPISCDDNNDCTIDNCDCKLGCIHKELPVDDFNNCTFDQCSHEEGI
THTPISCDDNNDCTTDSCDISIGCIHDQIPDCTINTSSNNSQNNPKDCIGENCINIPFCS
IKDKKCLELVEEIKKNQTSQFPPTSSSSSSHSQISKNKEIIIISTLFLGTLTXSLNFKXN
N
Translated Amino Acid sequence (All Frames) 
Frame A:
inistnnsikn*skriyysknen*tvsnknddytfihyfysigklptrk*nkcnnnnnnk
il***rimq**kfvyk*nv*ssywmyl*kyfm****wmykrfl*sidwmls*eggm****
*my**ym*s*dwnmli*kknmy****lhngl---

---nmy****lhnglv*sidrrmclstgfsl*rl**lyn*cl**iqglsif**nl***mc
lyn*yl*kfnwlyshsnim*rq**lyh**l*l*twlys*riar**f**lyi*pmfp*rry
nshsnfl***q*lyn**l*yfnwlyt*sns*lyy*yli**fsk*skrlyw*klykysilf
nqr*kvfrig*rnkkksnfsipsnffflfisfsdfkk*rnynniniifryfnxiskf*x*
**

Frame B:
*IYLQIIVLRTDQKEYIILKMKIKLCQIKMMIILLFIIFIQLGNCQLENKTNATTTTTTR
YCNNDESCNDENLCTNEMCDPVIGCIYENISCDDDNGCTKDFCDPLTGCFHKRVVCDDND
KCTDNICNPETGTCXFRRRICTDNNDCTMD---

---ICTDNNDCTMDWCNQLTGECVYQPVLVCEDYNSCTIDVCNEYKGCQFFNRTCDDRCV
CTIDTCKNSTGCIHTPISCDDNNDCTIDNCDCKLGCIHKELPVDDFNNCTFDQCSHEEGI
THTPISCDDNNDCTTDSCDISIGCIHDQIPDCTINTSSNNSQNNPKDCIGENCINIPFCS
IKDKKCLELVEEIKKNQTSQFPPTSSSSSSHSQISKNKEIIIISTLFLGTLTXSLNFKXN
N

Frame C:
kyiyk**y*elikknilf*k*klncvk*k**lyfyslflfnweian*kikqmqqqqqqqd
ivimtnhammkicvqmkcviqlldvfmkifhvmmimdvqkifvih*ldafirgwyvmimi
nvliiyvilrlehaxleeeyvliimiaqwt---

---yvliimiaqwtgvin*qenvfinrf*fvkiiivvqlmfvmntrvvnflielvmidvf
vqlilvkiqlvvftlqyhvttimivpliivivnlavfikncplmiliivhltnvpmkkv*
ltlqfpvmitmivqlivvifqlvvymikflivlliphliilkiiqkivlvkiv*ifhfvq
skiksv*nwlkk*kkikllnslqlllplhlilrfqkikkl**yqhyf*vl*hxl*ilxii
i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC228 (SFC228Q) /CSM/SF/SFC2-B/SFC228Q.Seq.d/                       1853   0.0
CFG115 (CFG115Q) /CSM/CF/CFG1-A/CFG115Q.Seq.d/                       1308   0.0
CFG644 (CFG644Q) /CSM/CF/CFG6-B/CFG644Q.Seq.d/                        852   0.0
SFA234 (SFA234Q) /CSM/SF/SFA2-B/SFA234Q.Seq.d/                        712   0.0
SFB849 (SFB849Q) /CSM/SF/SFB8-C/SFB849Q.Seq.d/                        547   e-154
SLE276 (SLE276Q) /CSM/SL/SLE2-D/SLE276Q.Seq.d/                         52   2e-05
SLE275 (SLE275Q) /CSM/SL/SLE2-D/SLE275Q.Seq.d/                         52   2e-05
SLE266 (SLE266Q) /CSM/SL/SLE2-C/SLE266Q.Seq.d/                         52   2e-05
SFI605 (SFI605Q) /CSM/SF/SFI6-A/SFI605Q.Seq.d/                         52   2e-05
SFF368 (SFF368Q) /CSM/SF/SFF3-C/SFF368Q.Seq.d/                         52   2e-05
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           40   4e-12  26
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    38   4e-12  24
AL929351|AL929351.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 1/4.    38   8e-10  22
AE014842|AE014842.1 Plasmodium falciparum 3D7 chromosome 11 section 7 of 8 of the complete sequence.    36   1e-09  22
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    46   2e-09  19
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    40   1e-08  18
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    36   3e-08  18
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            40   8e-08  26
AE014841|AE014841.1 Plasmodium falciparum 3D7 chromosome 11 section 6 of 8 of the complete sequence.    32   2e-07  18
AC006281|AC006281.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    34   3e-07  20
dna update 2003.12.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...   122   7e-45
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...   122   7e-45
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...   110   7e-42
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...   116   2e-41
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...   104   1e-37
A27020(A27020)DIF-induced prestalk pDd63 protein precursor - sli...   110   1e-32
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                 68   2e-18
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                 72   3e-17
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                 62   2e-16
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                 55   1e-12
protein update 2009. 5.22
PSORT 

 psg: 0.53  gvh: 0.71  alm: 0.24  top: 0.40  tms: 0.07  mit: 0.21  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.42  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 16.0 %: Golgi
	 16.0 %: nuclear
	 16.0 %: mitochondrial
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for SFC228 is cyt
5' end seq. ID SFC228F
5' end seq. 
>SFC228F.Seq
ATAAATATATCTACAAATAATAGTATTAAGAACTGATCAAAAAGAATATATTATTCTAAA
AATGAAAATTAAACTGTGTCAAATAAAAATGATGATTATACTTTTATTCATTATTTTTAT
TCAATTGGGAAATTGCCAACTAGAAAATAAAACAAATGCAACAACAACAACAACAACAAG
ATATTGTAATAATGACGAATCATGCAATGATGAAAATTTGTGTACAAATGAAATGTGTGA
TCCAGTTATTGGATGTATTTATGAAAATATTTCATGTGATGATGATAATGGATGTACAAA
AGATTTTTGTGATCCATTGACTGGATGCTTTCATAAGAGGGTGGTATGTGATGATAATGA
TAAATGTACTGATAATATATGTAATCCTGAGACTGGAACATGCTNATTTAGAAGAAGAAT
ATGTACTGATAATAATGATTGCACAATGGACTG----------
Length of 5' end seq. 453
3' end seq. ID SFC228Z
3' end seq. 
>SFC228Z.Seq
----------AATATGTACTGATAATAATGATTGCACAATGGACTGGTGTAATCAATTGA
CAGGAGAATGTGTTTATCAACCGGTTTTAGTTTGTGAAGATTATAATAGTTGTACAATTG
ATGTTTGTAATGAATACAAGGGTTGTCAATTTTTTAATAGAACTTGTGATGATAGATGTG
TTTGTACAATTGATACTTGTAAAAATTCAACTGGTTGTATTCACACTCCAATATCATGTG
ACGACAATAATGATTGTACCATTGATAATTGTGATTGTAAACTTGGCTGTATTCATAAAG
AATTGCCCGTTGATGATTTTAATAATTGTACATTTGACCAATGTTCCCATGAAGAAGGTA
TAACTCACACTCCAATTTCCTGTGATGATAACAATGATTGTACAACTGATAGTTGTGATA
TTTCAATTGGTTGTATACATGATCAAATTCCTGATTGTACTATTAATACCTCATCTAATA
ATTCTCAAAATAATCCAAAAGATTGTATTGGTGAAAATTGTATAAATATTCCATTTTGTT
CAATCAAAGATAAAAAGTGTTTAGAATTGGTTGAAGAAATAAAAAAAAATCAAACTTCTC
AATTCCCTCCAACTTCTTCTTCCTCTTCATCTCATTCTCAGATTTCAAAAAATAAAGAAA
TTATAATAATATCAACATTATTTTTAGGTACTTTAACATNATCTCTAAATTTTAAGNATA
ATAATAA
Length of 3' end seq. 717
Connected seq. ID SFC228P
Connected seq. 
>SFC228P.Seq
ATAAATATATCTACAAATAATAGTATTAAGAACTGATCAAAAAGAATATATTATTCTAAA
AATGAAAATTAAACTGTGTCAAATAAAAATGATGATTATACTTTTATTCATTATTTTTAT
TCAATTGGGAAATTGCCAACTAGAAAATAAAACAAATGCAACAACAACAACAACAACAAG
ATATTGTAATAATGACGAATCATGCAATGATGAAAATTTGTGTACAAATGAAATGTGTGA
TCCAGTTATTGGATGTATTTATGAAAATATTTCATGTGATGATGATAATGGATGTACAAA
AGATTTTTGTGATCCATTGACTGGATGCTTTCATAAGAGGGTGGTATGTGATGATAATGA
TAAATGTACTGATAATATATGTAATCCTGAGACTGGAACATGCTNATTTAGAAGAAGAAT
ATGTACTGATAATAATGATTGCACAATGGACTG----------AATATGTACTGATAATA
ATGATTGCACAATGGACTGGTGTAATCAATTGACAGGAGAATGTGTTTATCAACCGGTTT
TAGTTTGTGAAGATTATAATAGTTGTACAATTGATGTTTGTAATGAATACAAGGGTTGTC
AATTTTTTAATAGAACTTGTGATGATAGATGTGTTTGTACAATTGATACTTGTAAAAATT
CAACTGGTTGTATTCACACTCCAATATCATGTGACGACAATAATGATTGTACCATTGATA
ATTGTGATTGTAAACTTGGCTGTATTCATAAAGAATTGCCCGTTGATGATTTTAATAATT
GTACATTTGACCAATGTTCCCATGAAGAAGGTATAACTCACACTCCAATTTCCTGTGATG
ATAACAATGATTGTACAACTGATAGTTGTGATATTTCAATTGGTTGTATACATGATCAAA
TTCCTGATTGTACTATTAATACCTCATCTAATAATTCTCAAAATAATCCAAAAGATTGTA
TTGGTGAAAATTGTATAAATATTCCATTTTGTTCAATCAAAGATAAAAAGTGTTTAGAAT
TGGTTGAAGAAATAAAAAAAAATCAAACTTCTCAATTCCCTCCAACTTCTTCTTCCTCTT
CATCTCATTCTCAGATTTCAAAAAATAAAGAAATTATAATAATATCAACATTATTTTTAG
GTACTTTAACATNATCTCTAAATTTTAAGNATAATAATAA
Length of connected seq. 1170
Full length Seq ID -
Full length Seq. -
Length of full length seq. -