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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC181
Library SF
(Link to library)
Clone ID SFC181
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U13175-1|Contig-U15548-1
Original site URL
Representative seq. ID SFC181P
(Link to Original site)
Representative DNA sequence 
>SFC181 (SFC181Q) /CSM/SF/SFC1-D/SFC181Q.Seq.d/
ACAAATATAACAACTAATGGTGCCTCGGCACTAGGTGCTGAAAGTTCAACAACACCTTTA
CCATCATTAACAACTTTTTCACAATCACAATCTAGTTCACCACCAAATCAACCATCACCA
TCTATAACTCCACAACAAGTTTCAAATTTACCACCATCATATCAACCACCACAACCACCA
CCGACCATGGCACCACCACCATTATTTAATATTCCACAACAACAACAACAGCAACAAGTA
ACAAATAACAACAACAGTGGAGGTTATACACCACCACCATTACAATATACCCAATCTTCT
TCAAATTTACCACCAATTCAATTAGGTGTAACTAATTCACCTTCAAAACCACAATTATCA
GATAAACAAAAGGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAGGAAAGA
GAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAGGAAAAAGAAAAAGAAAAG
AAGGGACATAAAAAGTCATCATCATCAACATCACCAAATTCTTCCAGNTTATCAATTTCA
AACTTTTTATCAAGTAACAAGGATAAGGATAAAGAAAAAGATAAAGAAAAAGAAAAAGAA
AAAGAAAAAGAAAAAGATAAAGAAATATTAGCGACTAATTCAACACCAGAAAAACCAGGT
TCAAATCGTATGTCATTAATATTTAGTCAACAATTACAACAACAATTACAGCAACAGATT
CAACAACATCAACAATTXXXXXXXXXXCCNCCCCCCCCCTNCCAAAGNTTAGAAAAAAAA
TNAAAACNAAACCCAAAACTNTGGNTCCCTATNAAAAAAAAAAAACAAAACATATATCAT
ATAAGTGTTAAACACTCATCATAAAAAATATATATATATATAGATCCCTCCACACAACTC
AATA
sequence update 2003. 5. 2
Translated Amino Acid sequence 
TNITTNGASALGAESSTTPLPSLTTFSQSQSSSPPNQPSPSITPQQVSNLPPSYQPPQPP
PTMAPPPLFNIPQQQQQQQVTNNNNSGGYTPPPLQYTQSSSNLPPIQLGVTNSPSKPQLS
DKQKEKEKEKEKEKEKEKEREKEKEKEKEKEKEKEKEKEKKGHKKSSSSTSPNSSXLSIS
NFLSSNKDKDKEKDKEKEKEKEKEKDKEILATNSTPEKPGSNRMSLIFSQQLQQQLQQQI
QQHQQ---

---PPPPXQXLEKKXKXNPKLWXPXKKKKQNIYHISVKHSS*kiyiyidpstqln
Translated Amino Acid sequence (All Frames) 
Frame A:
TNITTNGASALGAESSTTPLPSLTTFSQSQSSSPPNQPSPSITPQQVSNLPPSYQPPQPP
PTMAPPPLFNIPQQQQQQQVTNNNNSGGYTPPPLQYTQSSSNLPPIQLGVTNSPSKPQLS
DKQKEKEKEKEKEKEKEKEREKEKEKEKEKEKEKEKEKEKKGHKKSSSSTSPNSSXLSIS
NFLSSNKDKDKEKDKEKEKEKEKEKDKEILATNSTPEKPGSNRMSLIFSQQLQQQLQQQI
QQHQQ---

---PPPPXQXLEKKXKXNPKLWXPXKKKKQNIYHISVKHSS*kiyiyidpstqln

Frame B:
qi*qlmvprh*vlkvqqhlyhh*qlfhnhnlvhhqinhhhl*lhnkfqiyhhhinhhnhh
rpwhhhhylifhnnnnsnk*qitttvevihhhhynipnllqiyhqfn*v*lihlqnhnyq
inkrkkkkkkkkkkkkkrkekkkkkkkkkkkkkrkkkkkrrdikshhhqhhqilpxyqfq
tfyqvtririkkkikkkkkkkkkkkikky*rliqhqknqvqivch*ylvnnynnnysnrf
nninn---

---xpppxkx*kknxnxtqnxgslxkkknktyii*vlnthhkkyiyi*ipphnsi

Frame C:
kynn*wclgtrc*kfnntftiinnfftiti*fttkstitiynsttsfkfttiistttttt
dhgtttii*ystttttatsnk*qqqwrlytttitiypiffkfttnsircn*ftfkttiir
*tkgkrkrkrkrkrkrkgkrkrkrkrkrkrkrkgkrkrkegt*kviiinitkffqxinfk
lfik*qg*g*rkr*rkrkrkrkrkr*rnisd*fntrktrfksyvini*stitttitatds
ttsti---

---xpplpkxrkkxktkpktxxpyxkkktkhisykc*tliikniyiyrslhttq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC181 (SFC181Q) /CSM/SF/SFC1-D/SFC181Q.Seq.d/                        325   7e-88
CHR719 (CHR719Q) /CSM/CH/CHR7-A/CHR719Q.Seq.d/                        109   7e-23
AFC464 (AFC464Q) /CSM/AF/AFC4-C/AFC464Q.Seq.d/                         78   3e-13
CHF745 (CHF745Q) /CSM/CH/CHF7-B/CHF745Q.Seq.d/                         46   0.001
CHF325 (CHF325Q) /CSM/CH/CHF3-B/CHF325Q.Seq.d/                         46   0.001
CHH171 (CHH171Q) /CSM/CH/CHH1-C/CHH171Q.Seq.d/                         44   0.004
VHG141 (VHG141Q) /CSM/VH/VHG1-B/VHG141Q.Seq.d/                         42   0.015
SSD223 (SSD223Q) /CSM/SS/SSD2-A/SSD223Q.Seq.d/                         42   0.015
SFE821 (SFE821Q) /CSM/SF/SFE8-A/SFE821Q.Seq.d/                         42   0.015
VHO877 (VHO877Q) /CSM/VH/VHO8-D/VHO877Q.Seq.d/                         38   0.23
own update 2003. 5.21
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ543068|AZ543068.1 ENTEW09TR Entamoeba histolytica Sheared DNA Entamoeba histolyticagenomic, genomic survey sequence.    46   0.002  3
AY160106|AY160106.1 Dictyostelium discoideum nucleotide exchange factor RasGEF R (gefR) gene, complete cds.    34   0.32   4
BX664613|BX664613.17 Zebrafish DNA sequence from clone DKEY-5P5 in linkage group 22.    40   0.32   3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    34   0.38   12
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    36   0.45   11
AC019988|AC019988.1 Drosophila melanogaster, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    36   0.67   6
AY085122|AY085122.1 Arabidopsis thaliana clone 13188 mRNA, complete sequence.    34   0.94   3
AJ801468|AJ801468.1 Antirrhinum majus EST, clone 018_5_01_e20.     46   0.99   1
BQ090175|BQ090175.1 rc62d10.y1 Meloidogyne hapla egg pAMP1 v1 Meloidogyne hapla cDNA 5' similar to TR:Q23117 Q23117 W01C9.5 PROTEIN. [1] ;, mRNA sequence.    46   0.99   1
BQ090172|BQ090172.1 rc62d06.y1 Meloidogyne hapla egg pAMP1 v1 Meloidogyne hapla cDNA 5' similar to TR:Q23117 Q23117 W01C9.5 PROTEIN. [1] ;, mRNA sequence.    46   0.99   1
dna update 2004. 9.21
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.311    0.130    0.385

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 747,936,770
protein update 2009. 4.14
PSORT 

 psg: 0.81  gvh: 0.61  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.51  mip: 0.00
 nuc: 0.18  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 32.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for SFC181 is mit
5' end seq. ID SFC181F
5' end seq. 
>SFC181F.Seq
ACAAATATAACAACTAATGGTGCCTCGGCACTAGGTGCTGAAAGTTCAACAACACCTTTA
CCATCATTAACAACTTTTTCACAATCACAATCTAGTTCACCACCAAATCAACCATCACCA
TCTATAACTCCACAACAAGTTTCAAATTTACCACCATCATATCAACCACCACAACCACCA
CCGACCATGGCACCACCACCATTATTTAATATTCCACAACAACAACAACAGCAACAAGTA
ACAAATAACAACAACAGTGGAGGTTATACACCACCACCATTACAATATACCCAATCTTCT
TCAAATTTACCACCAATTCAATTAGGTGTAACTAATTCACCTTCAAAACCACAATTATCA
GATAAACAAAAGGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAGGAAAGA
GAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAGGAAAAAGAAAAAGAAAAG
AAGGGACATAAAAAGTCATCATCATCAACATCACCAAATTCTTCCAGNTTATCAATTTCA
AACTTTTTATCAAGTAACAAGGATAAGGATAAAGAAAAAGATAAAGAAAAAGAAAAAGAA
AAAGAAAAAGAAAAAGATAAAGAAATATTAGCGACTAATTCAACACCAGAAAAACCAGGT
TCAAATCGTATGTCATTAATATTTAGTCAACAATTACAACAACAATTACAGCAACAGATT
CAACAACATCAACAATTNNNNNNNNNN
Length of 5' end seq. 747
3' end seq. ID SFC181Z
3' end seq. 
>SFC181Z.Seq
----------CCNCCCCCCCCCTNCCAAAGNTTAGAAAAAAAATNAAAACNAAACCCAAA
ACTNTGGNTCCCTATNAAAAAAAAAAAACAAAACATATATCATATAAGTGTTAAACACTC
ATCATAAAAAATATATATATATATAGATCCCTCCACACAACTCAATA
Length of 3' end seq. 157
Connected seq. ID SFC181P
Connected seq. 
>SFC181P.Seq
ACAAATATAACAACTAATGGTGCCTCGGCACTAGGTGCTGAAAGTTCAACAACACCTTTA
CCATCATTAACAACTTTTTCACAATCACAATCTAGTTCACCACCAAATCAACCATCACCA
TCTATAACTCCACAACAAGTTTCAAATTTACCACCATCATATCAACCACCACAACCACCA
CCGACCATGGCACCACCACCATTATTTAATATTCCACAACAACAACAACAGCAACAAGTA
ACAAATAACAACAACAGTGGAGGTTATACACCACCACCATTACAATATACCCAATCTTCT
TCAAATTTACCACCAATTCAATTAGGTGTAACTAATTCACCTTCAAAACCACAATTATCA
GATAAACAAAAGGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAGGAAAGA
GAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAAGAAAAGGAAAAAGAAAAAGAAAAG
AAGGGACATAAAAAGTCATCATCATCAACATCACCAAATTCTTCCAGNTTATCAATTTCA
AACTTTTTATCAAGTAACAAGGATAAGGATAAAGAAAAAGATAAAGAAAAAGAAAAAGAA
AAAGAAAAAGAAAAAGATAAAGAAATATTAGCGACTAATTCAACACCAGAAAAACCAGGT
TCAAATCGTATGTCATTAATATTTAGTCAACAATTACAACAACAATTACAGCAACAGATT
CAACAACATCAACAATT----------CCNCCCCCCCCCTNCCAAAGNTTAGAAAAAAAA
TNAAAACNAAACCCAAAACTNTGGNTCCCTATNAAAAAAAAAAAACAAAACATATATCAT
ATAAGTGTTAAACACTCATCATAAAAAATATATATATATATAGATCCCTCCACACAACTC
AATA
Length of connected seq. 894
Full length Seq ID -
Full length Seq. -
Length of full length seq. -