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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC169
Library SF
(Link to library)
Clone ID SFC169
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SFC169P
(Link to Original site)
Representative DNA sequence 
>SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/
ATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCAT
CAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGA
CCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGC
ATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGG
TAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGG
TAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCC
AAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGC
TGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAA
AATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAA
ATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGXXXXXXXXXXCTTAAAGAATGT
CATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCC
ACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGG
TTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAA
TGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGA
AAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGA
TCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCA
AATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAA
GAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAAT
TCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGG
TGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAA
AACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAA
CGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTA
CTCATAATATGNAAACAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGA
STGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTP
NKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNK
LAMQEFMILPVG---

---LKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAA
SEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKL
TASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGV
MVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLT
FRKF*fys*yxnk
Translated Amino Acid sequence (All Frames) 
Frame A:
itlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssirc
inwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwyp
k*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**
isyariydftsw---

---lkechqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglcc
l*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*i
nsis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgc
hg*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lcwfn
fp*ililliixkq

Frame B:
LLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGA
STGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTP
NKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNK
LAMQEFMILPVG---

---LKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAA
SEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKL
TASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGV
MVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLT
FRKF*fys*yxnk

Frame C:
yfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvh
qlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpq
ikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin
*lcknl*fyqlv---

---*rmssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlp
lnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*
qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvs
wlaivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitmlv*l
svnfnfthnmxtk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       2555   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1485   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1477   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1473   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1473   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1471   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1471   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       1471   0.0
SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       1469   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1461   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   3e-76  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   2e-72  11
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-61  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-61  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   1e-61  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   1e-59  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   4e-53  5
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121   2e-48  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66   5e-46  6
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   2e-45  10
dna update 2003.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   804   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   581   e-164
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   570   e-161
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   562   e-158
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   560   e-158
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   560   e-158
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   560   e-158
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   560   e-158
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   558   e-157
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   557   e-157
protein update 2009. 5.22
PSORT 

 psg: 0.95  gvh: 0.47  alm: 0.33  top: 0.60  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SFC169 is cyt
5' end seq. ID SFC169F
5' end seq. 
>SFC169F.Seq
ATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCAT
CAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGA
CCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGC
ATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGG
TAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGG
TAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCC
AAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGC
TGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAA
AATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAA
ATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTG----------
Length of 5' end seq. 578
3' end seq. ID SFC169Z
3' end seq. 
>SFC169Z.Seq
----------CTTAAAGAATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTG
GTGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAA
AACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTG
CCTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACG
GTTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAAT
ATCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAAT
TAACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAA
GAATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAA
TTGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTG
TCATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTG
GTTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAAT
ACAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAA
CTTTCCGTAAATTTTAATTTTACTCATAATATGNAAACAAAA
Length of 3' end seq. 752
Connected seq. ID SFC169P
Connected seq. 
>SFC169P.Seq
ATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCAT
CAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGA
CCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGC
ATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGG
TAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGG
TAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCC
AAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGC
TGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAA
AATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAA
ATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTG----------CTTAAAGAATGT
CATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCC
ACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGG
TTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAA
TGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGA
AAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGA
TCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCA
AATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAA
GAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAAT
TCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGG
TGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAA
AACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAA
CGAAGAGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTA
CTCATAATATGNAAACAAAA
Length of connected seq. 1330
Full length Seq ID -
Full length Seq. -
Length of full length seq. -