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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFC128
Library SF
(Link to library)
Clone ID SFC128
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16513-1
Original site URL
Representative seq. ID SFC128P
(Link to Original site)
Representative DNA sequence 
>SFC128 (SFC128Q) /CSM/SF/SFC1-B/SFC128Q.Seq.d/
ACTGTTGGCCTACTGGATTTCATTTATTAATAATACATCCACAAAGAATGAAAATATTAT
TATTTGTTACATTAATTGCACTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTG
TTGTTTTATCACCAGACAACTTTGATACAGATGTTGATGGTTCAAAAACTGTATTTGTAA
AANTCTATGCACCATGGXXXXXXXXXXAAAAGATGTTGTCATCGCCAAAATTGATTGCGA
TGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAA
ATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATAC
TTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGC
TGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGC
TGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGA
AGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATC
AATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTC
TGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATCAAA
ATAAATTAAATTTAATTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
CWPTGFHLLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTDVDGSKTVFVK
XYAPW---

---KDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDGEKYEQGRDLDTFINYIN
KQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVKKAQTVVDSLPEELRTEG
SYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEFAKKLNILESFKSK*ikfn
Translated Amino Acid sequence (All Frames) 
Frame A:
tvglldfiy**yihke*kyyyllh*lhwhllpyaqqkemllfyhqttliqmlmvqklyl*
xsmhh---

---krcchrqn*lrcc**qshllqiwchwfpntqmvr*tkqrr*ki*trs*s*yfhqlhq
qtswc*pcqrw*trcwcwsc*ttryych*ihrcrc*sp*gtrqessnrr*fsprrikn*r
filrqshenhc*kinrfcnh*nrsyhqislwfnvwkes**icqetqyfrifqikin*i*f

Frame B:
llaywisfinntstkneniiicyinctgicclmlsrrkcccfitrql*yrc*wfkncick
xlctm---

---KDVVIAKIDCDAADNKAICSKYGVTGFPTLKWFGKQSKDGEKYEQGRDLDTFINYIN
KQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAAEVRKELVKKAQTVVDSLPEELRTEG
SYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMSGKKADEFAKKLNILESFKSK*ikfn

Frame C:
CWPTGFHLLIIHPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTDVDGSKTVFVK
XYAPW---

---kmlsspkliamllitkpsapnmvslvsqhsngsvnkaktvknmnkvvililssttst
nklvltvskvvnsllvlvvlnnsillplnsslpllksvrnssrklkpslilsqkn*elkv
httsks*kpllknqsil*plkslvspn*slvqclerklmnlprnsif*nlsnqnklnli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF163 (VSF163Q) /CSM/VS/VSF1-C/VSF163Q.Seq.d/                       1053   0.0
VFN233 (VFN233Q) /CSM/VF/VFN2-B/VFN233Q.Seq.d/                       1053   0.0
VFN206 (VFN206Q) /CSM/VF/VFN2-A/VFN206Q.Seq.d/                       1053   0.0
VFM733 (VFM733Q) /CSM/VF/VFM7-B/VFM733Q.Seq.d/                       1053   0.0
VFM578 (VFM578Q) /CSM/VF/VFM5-D/VFM578Q.Seq.d/                       1053   0.0
VFM537 (VFM537Q) /CSM/VF/VFM5-B/VFM537Q.Seq.d/                       1053   0.0
VFM151 (VFM151Q) /CSM/VF/VFM1-C/VFM151Q.Seq.d/                       1053   0.0
VFL584 (VFL584Q) /CSM/VF/VFL5-D/VFL584Q.Seq.d/                       1053   0.0
VFL481 (VFL481Q) /CSM/VF/VFL4-D/VFL481Q.Seq.d/                       1053   0.0
VFL139 (VFL139Q) /CSM/VF/VFL1-B/VFL139Q.Seq.d/                       1053   0.0
own update 2001.11.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  1045   0.0    2
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1045   0.0    11
AF222718|AF222718.1 Chrysodidymus synuroideus mitochondrion, complete genome.    40   2e-04  5
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    36   8e-04  9
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   0.001  16
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    34   0.002  13
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    36   0.004  12
AL928622|AL928622.9 Zebrafish DNA sequence from clone DKEY-31O13.    36   0.007  2
BX248111|BX248111.7 Zebrafish DNA sequence from clone DKEY-22L11 in linkage group 4.    36   0.007  2
BX649444|BX649444.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEYP-27E10.    36   0.007  2
dna update 2003.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   308   1e-88
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   306   7e-88
AB182631_1(AB182631|pid:none) Glycine max Gm pdis-2 mRNA for pro...   114   6e-31
AY739288_1(AY739288|pid:none) Zea mays protein disulfide isomera...   114   2e-29
EU966127_1(EU966127|pid:none) Zea mays clone 291908 PDIL2-2 - Ze...   114   2e-29
AY730725_1(AY730725|pid:none) Entamoeba histolytica protein disu...   114   2e-28
AE017341_67(AE017341|pid:none) Cryptococcus neoformans var. neof...   127   3e-28
AC122168_31(AC122168|pid:none) Medicago truncatula clone mth2-4o...   109   2e-27
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   120   3e-26
EU284948_1(EU284948|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   119   9e-26
protein update 2009. 5.22
PSORT 

 psg: 0.91  gvh: 0.77  alm: 0.48  top: 0.07  tms: 0.00  mit: 0.30  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for SFC128 is exc
5' end seq. ID SFC128F
5' end seq. 
>SFC128F.Seq
ACTGTTGGCCTACTGGATTTCATTTATTAATAATACATCCACAAAGAATGAAAATATTAT
TATTTGTTACATTAATTGCACTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTG
TTGTTTTATCACCAGACAACTTTGATACAGATGTTGATGGTTCAAAAACTGTATTTGTAA
AANTCTATGCACCATGG----------
Length of 5' end seq. 197
3' end seq. ID SFC128Z
3' end seq. 
>SFC128Z.Seq
----------AAAAGATGTTGTCATCGCCAAAATTGATTGCGATGCTGCTGATAACAAAG
CCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAAATGGTTCGGTAAACAAA
GCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTCATCAACTACATCA
ACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTTGGTGCTGGTCGTG
TTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGCTGCTGAAGTCCGTAAGG
AACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAATTAAGAACTGAAG
GTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATCGATTTTGTAACCA
CTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTCTGGAAAGAAAGCTGATG
AATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATCAAAATAAATTAAATTTAATT
T
Length of 3' end seq. 531
Connected seq. ID SFC128P
Connected seq. 
>SFC128P.Seq
ACTGTTGGCCTACTGGATTTCATTTATTAATAATACATCCACAAAGAATGAAAATATTAT
TATTTGTTACATTAATTGCACTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTTG
TTGTTTTATCACCAGACAACTTTGATACAGATGTTGATGGTTCAAAAACTGTATTTGTAA
AANTCTATGCACCATGG----------AAAAGATGTTGTCATCGCCAAAATTGATTGCGA
TGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAA
ATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATAC
TTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGC
TGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGC
TGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGA
AGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATC
AATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTC
TGGAAAGAAAGCTGATGAATTTGCCAAGAAACTCAATATTTTAGAATCTTTCAAATCAAA
ATAAATTAAATTTAATTT
Length of connected seq. 728
Full length Seq ID -
Full length Seq. -
Length of full length seq. -