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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFB864
Library SF
(Link to library)
Clone ID SFB864
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16501-1
Original site URL
Representative seq. ID SFB864P
(Link to Original site)
Representative DNA sequence 
>SFB864 (SFB864Q) /CSM/SF/SFB8-C/SFB864Q.Seq.d/
ATGCAAATTACAGAAAAAGAAACAGTACAAGAAGAACAACAAAAATTACTATATCCACCA
CCTTCTTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATAATGAAATGTATAAA
GAAAGTATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAGAATTTTTAACTTGG
TTTTCTGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTGATATTTCATGGTTT
TTAAATGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATTTAAAAGAGAATGCA
GATAAAGTTGCAATACTATTTGAAGGTGATGAAGAAACAATGGTTAAAAAAGTTACATAT
AGAGAGATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATATCACTTGGCGTTGGT
AAAGGAGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCATTTATTCAATGTTA
GCATGCGCTAGAATTGGAGCAATTCATTCAGTTATTTTTGCAGGATTTGGTTATGAATCC
ATTGTTTCACGTGTTCATGATGCAAAATGTAGAGTGATCATAACGGCTGATGAANGXXXX
XXXXXXTTTGGTATAGAATTACAAGTTTTAGACTCAAAAACTGGTGAACGTTTATATATT
AATCCCGATATCAATGGTTGTAAAGAAATTTCTGGTGTATTGGCAATCTCTAAACCTTGG
CCAGGTATTGCTCGTTCAGTTTATCGTTCTCATGGTCGTTATCTTCAAACTTATATGACT
CAATATAAAGGTCACTATTTCACTGGTGATGGTGTAAAACTTGACTCTGATGGTTATTAT
TGGATCGAAGGTAGAGTAGATGATGTTATCAATGTTAGTGGTCATAGATTAGGTACTGCT
GAATTAGAGAGTGCCCTCGTTGGTTGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTAT
CCTCATGATATCAAAGGTCAAGGTATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAA
GAGGATGAATCAAATGTTATTATGATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCA
TTTGCAACACCTGATGTAATTGTTATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAA
ATTATGAGAAGAATTCTTAGAAAAATTGGTTGTCATGAATCTTCTGCTGAACAATTAGGT
GATATTTCAACTTTAGCTGAACCTGAAGTTGTCAAACTTTTAATTGAAAAAGTTTCAAAA
GTAATTCCAAAAACTCATTAAAAAAATAAAAAATAAAAAAAAATAAATTCAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
MQITEKETVQEEQQKLLYPPPSFSTKCHISSVEQYNEMYKESIESPNQFWDKKAKEFLTW
FSDYTTVQHGSFEKGDISWFLNGKINVSYNCIDRHLKENADKVAILFEGDEETMVKKVTY
REMFEEVCRLSNLLISLGVGKGDTVAIYLPNTPTAIYSMLACARIGAIHSVIFAGFGYES
IVSRVHDAKCRVIITADE---

---FGIELQVLDSKTGERLYINPDINGCKEISGVLAISKPWPGIARSVYRSHGRYLQTYM
TQYKGHYFTGDGVKLDSDGYYWIEGRVDDVINVSGHRLGTAELESALVGCSICAEAAVVG
YPHDIKGQGILAFCTLKEGYQEDESNVIMMLKKEVRNVIGPFATPDVIVITPSLPKTRSG
KIMRRILRKIGCHESSAEQLGDISTLAEPEVVKLLIEKVSKVIPKTH*knkk*kkins
Translated Amino Acid sequence (All Frames) 
Frame A:
MQITEKETVQEEQQKLLYPPPSFSTKCHISSVEQYNEMYKESIESPNQFWDKKAKEFLTW
FSDYTTVQHGSFEKGDISWFLNGKINVSYNCIDRHLKENADKVAILFEGDEETMVKKVTY
REMFEEVCRLSNLLISLGVGKGDTVAIYLPNTPTAIYSMLACARIGAIHSVIFAGFGYES
IVSRVHDAKCRVIITADE---

---FGIELQVLDSKTGERLYINPDINGCKEISGVLAISKPWPGIARSVYRSHGRYLQTYM
TQYKGHYFTGDGVKLDSDGYYWIEGRVDDVINVSGHRLGTAELESALVGCSICAEAAVVG
YPHDIKGQGILAFCTLKEGYQEDESNVIMMLKKEVRNVIGPFATPDVIVITPSLPKTRSG
KIMRRILRKIGCHESSAEQLGDISTLAEPEVVKLLIEKVSKVIPKTH*knkk*kkins

Frame B:
cklqkkkqykknnknyyihhlllvqnviyhq*nnimkcikkvlnhqinfgikklknf*lg
fliiqlfnmdhskkvifhgf*mvklmfhiivsidi*krmqiklqyylkvmkkqwlkklhi
erclkrfvdyqif*yhlalvkeir*rsiyqthqppfiqc*haleleqfiqlflqdlvmnp
lfhvfmmqnve*s*rlmx---

---lv*nykf*tqklvnvyilipismvvkkflvywqslnlgqvllvqfivlmvvifkli*
lnikvtislvmv*nltlmviigskve*mmlsmlvvid*vlln*rvpslvvqfvqrlpllv
ilmiskvkvf*hfvhskkvikrmnqmll*c*kkklem*lvhlqhlm*ll*hlhfqklvvv
kl*eefleklvvmnlllnn*vifql*lnlklsnf*lkkfqk*fqklikkiknkkk*iq

Frame C:
anyrkrnstrrttkitisttff*ykmsyiisrti**nv*rky*itksilg*ks*rifnlv
f*lyncstwiirkr*yfmvfkw*n*cfi*lyr*tfkrecr*scnti*r**rnng*ksyi*
rdv*rgl*iiksfditwrw*rrygsdlstkhtnrhlfnvsmr*nwsnsfsyfcriwl*ih
cftcs*ckm*sdhng**x---

---wyritsfrlknw*tfiy*sryqwl*rnfwcignl*tlarycsfslsfswslssnlyd
si*rslfhw*wckt*l*wllldrr*sr*cyqc*ws*iryc*irecprwlfnlcrgcrcwl
ss*yqrsryfsilytqrrlsrg*ikcyydvkkrs*kcnwsicnt*cncyntftskns*w*
nyekns*knwls*ifc*tir*yfnfs*t*scqtfn*ksfksnsknslkk*kikknkf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFB864 (SFB864Q) /CSM/SF/SFB8-C/SFB864Q.Seq.d/                       2428   0.0
VHJ869 (VHJ869Q) /CSM/VH/VHJ8-C/VHJ869Q.Seq.d/                       1283   0.0
VHO673 (VHO673Q) /CSM/VH/VHO6-D/VHO673Q.Seq.d/                       1271   0.0
SFF506 (SFF506Q) /CSM/SF/SFF5-A/SFF506Q.Seq.d/                       1271   0.0
AFE757 (AFE757Q) /CSM/AF/AFE7-C/AFE757Q.Seq.d/                       1271   0.0
CFJ158 (CFJ158Q) /CSM/CF/CFJ1-C/CFJ158Q.Seq.d/                       1263   0.0
VHO771 (VHO771Q) /CSM/VH/VHO7-C/VHO771Q.Seq.d/                       1249   0.0
VHE704 (VHE704Q) /CSM/VH/VHE7-A/VHE704Q.Seq.d/                       1249   0.0
CHF658 (CHF658Q) /CSM/CH/CHF6-C/CHF658Q.Seq.d/                       1247   0.0
CFH854 (CFH854Q) /CSM/CF/CFH8-C/CFH854Q.Seq.d/                       1237   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.   878   0.0    6
AX489103|AX489103.1 Sequence 6403 from Patent WO02053728.          70   1e-23  6
AZ930496|AZ930496.1 474.dhz54h09.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz54h09.s1, DNA sequence.    72   4e-14  2
CD978883|CD978883.1 QAF6c12.yg QAF Zea mays cDNA clone QAF6c12, mRNA sequence.    42   2e-12  4
AW037040|AW037040.1 614025A12.y1 614 - root cDNA library from Walbot Lab Zea mays cDNA, mRNA sequence.    42   5e-12  4
BI674812|BI674812.1 949067E10.y2 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42   1e-11  4
CA269673|CA269673.1 SCMCRT3083F09.g RT3 Saccharum officinarum cDNA clone SCMCRT3083F09 5', mRNA sequence.    42   2e-11  4
CA258874|CA258874.1 SCBGRT3013E01.g RT3 Saccharum officinarum cDNA clone SCBGRT3013E01 5', mRNA sequence.    42   2e-11  4
CF635493|CF635493.1 zmrww00_0A20-011-b10.s2 zmrww00 Zea mays cDNA, mRNA sequence.    42   3e-11  4
BI478599|BI478599.1 949067E10.x1 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence.    42   2e-10  4
dna update 2003.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115612_37(AC115612|pid:none) Dictyostelium discoideum chromoso...   454   e-126
(Q54Z60) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   454   e-126
AL832939_1(AL832939|pid:none) Homo sapiens mRNA; cDNA DKFZp666G0...   303   7e-81
DQ782753_1(DQ782753|pid:none) Polytomella parva plastid acetyl C...   228   4e-58
(Q6MNF1) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   226   2e-57
CP000158_1385(CP000158|pid:none) Hyphomonas neptunium ATCC 15444...   225   2e-57
CR940353_190(CR940353|pid:none) Theileria annulata strain Ankara...   225   3e-57
CP000157_932(CP000157|pid:none) Erythrobacter litoralis HTCC2594...   224   4e-57
(Q056J9) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   224   7e-57
CP000362_2174(CP000362|pid:none) Roseobacter denitrificans OCh 1...   223   1e-56
protein update 2009. 5.22
PSORT 

 psg: 0.75  gvh: 0.32  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	 20.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for SFB864 is cyt
5' end seq. ID SFB864F
5' end seq. 
>SFB864F.Seq
ATGCAAATTACAGAAAAAGAAACAGTACAAGAAGAACAACAAAAATTACTATATCCACCA
CCTTCTTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATAATGAAATGTATAAA
GAAAGTATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAGAATTTTTAACTTGG
TTTTCTGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTGATATTTCATGGTTT
TTAAATGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATTTAAAAGAGAATGCA
GATAAAGTTGCAATACTATTTGAAGGTGATGAAGAAACAATGGTTAAAAAAGTTACATAT
AGAGAGATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATATCACTTGGCGTTGGT
AAAGGAGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCATTTATTCAATGTTA
GCATGCGCTAGAATTGGAGCAATTCATTCAGTTATTTTTGCAGGATTTGGTTATGAATCC
ATTGTTTCACGTGTTCATGATGCAAAATGTAGAGTGATCATAACGGCTGATGAANG----
------
Length of 5' end seq. 596
3' end seq. ID SFB864Z
3' end seq. 
>SFB864Z.Seq
----------TTTGGTATAGAATTACAAGTTTTAGACTCAAAAACTGGTGAACGTTTATA
TATTAATCCCGATATCAATGGTTGTAAAGAAATTTCTGGTGTATTGGCAATCTCTAAACC
TTGGCCAGGTATTGCTCGTTCAGTTTATCGTTCTCATGGTCGTTATCTTCAAACTTATAT
GACTCAATATAAAGGTCACTATTTCACTGGTGATGGTGTAAAACTTGACTCTGATGGTTA
TTATTGGATCGAAGGTAGAGTAGATGATGTTATCAATGTTAGTGGTCATAGATTAGGTAC
TGCTGAATTAGAGAGTGCCCTCGTTGGTTGTTCAATTTGTGCAGAGGCTGCCGTTGTTGG
TTATCCTCATGATATCAAAGGTCAAGGTATTTTAGCATTTTGTACACTCAAAGAAGGTTA
TCAAGAGGATGAATCAAATGTTATTATGATGTTAAAAAAAGAAGTTAGAAATGTAATTGG
TCCATTTGCAACACCTGATGTAATTGTTATAACACCTTCACTTCCAAAAACTCGTAGTGG
TAAAATTATGAGAAGAATTCTTAGAAAAATTGGTTGTCATGAATCTTCTGCTGAACAATT
AGGTGATATTTCAACTTTAGCTGAACCTGAAGTTGTCAAACTTTTAATTGAAAAAGTTTC
AAAAGTAATTCCAAAAACTCATTAAAAAAATAAAAAATAAAAAAAAATAAATTCAG
Length of 3' end seq. 706
Connected seq. ID SFB864P
Connected seq. 
>SFB864P.Seq
ATGCAAATTACAGAAAAAGAAACAGTACAAGAAGAACAACAAAAATTACTATATCCACCA
CCTTCTTTTAGTACAAAATGTCATATATCATCAGTAGAACAATATAATGAAATGTATAAA
GAAAGTATTGAATCACCAAATCAATTTTGGGATAAAAAAGCTAAAGAATTTTTAACTTGG
TTTTCTGATTATACAACTGTTCAACATGGATCATTCGAAAAAGGTGATATTTCATGGTTT
TTAAATGGTAAAATTAATGTTTCATATAATTGTATCGATAGACATTTAAAAGAGAATGCA
GATAAAGTTGCAATACTATTTGAAGGTGATGAAGAAACAATGGTTAAAAAAGTTACATAT
AGAGAGATGTTTGAAGAGGTTTGTAGATTATCAAATCTTTTGATATCACTTGGCGTTGGT
AAAGGAGATACGGTAGCGATCTATCTACCAAACACACCAACCGCCATTTATTCAATGTTA
GCATGCGCTAGAATTGGAGCAATTCATTCAGTTATTTTTGCAGGATTTGGTTATGAATCC
ATTGTTTCACGTGTTCATGATGCAAAATGTAGAGTGATCATAACGGCTGATGAANG----
------TTTGGTATAGAATTACAAGTTTTAGACTCAAAAACTGGTGAACGTTTATATATT
AATCCCGATATCAATGGTTGTAAAGAAATTTCTGGTGTATTGGCAATCTCTAAACCTTGG
CCAGGTATTGCTCGTTCAGTTTATCGTTCTCATGGTCGTTATCTTCAAACTTATATGACT
CAATATAAAGGTCACTATTTCACTGGTGATGGTGTAAAACTTGACTCTGATGGTTATTAT
TGGATCGAAGGTAGAGTAGATGATGTTATCAATGTTAGTGGTCATAGATTAGGTACTGCT
GAATTAGAGAGTGCCCTCGTTGGTTGTTCAATTTGTGCAGAGGCTGCCGTTGTTGGTTAT
CCTCATGATATCAAAGGTCAAGGTATTTTAGCATTTTGTACACTCAAAGAAGGTTATCAA
GAGGATGAATCAAATGTTATTATGATGTTAAAAAAAGAAGTTAGAAATGTAATTGGTCCA
TTTGCAACACCTGATGTAATTGTTATAACACCTTCACTTCCAAAAACTCGTAGTGGTAAA
ATTATGAGAAGAATTCTTAGAAAAATTGGTTGTCATGAATCTTCTGCTGAACAATTAGGT
GATATTTCAACTTTAGCTGAACCTGAAGTTGTCAAACTTTTAATTGAAAAAGTTTCAAAA
GTAATTCCAAAAACTCATTAAAAAAATAAAAAATAAAAAAAAATAAATTCAG
Length of connected seq. 1302
Full length Seq ID -
Full length Seq. -
Length of full length seq. -