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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFB846
Library SF
(Link to library)
Clone ID SFB846
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SFB846P
(Link to Original site)
Representative DNA sequence 
>SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/
TTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTC
ATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTT
GACCTTTACACCGAAAAGGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGT
GCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTA
GGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATT
GGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACC
CCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGA
GCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACT
AAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAAT
AAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGXXXXXXXXXXTTAAAGAATGTC
ATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCA
CCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGT
TACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAAT
GGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAA
AAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGAT
CCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAA
ATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAG
AAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATT
CGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGT
GAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAA
ACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAAC
GAAGAGTTAGGTGAAAACCATAACTATGCTNGTTTAACTTTCCGTAAATTTTAATTTTAC
TCATAATATGTAATCAAAAC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILPV---

---LKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAA
SEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKL
TASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGV
MVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAXLT
FRKF*fys*yvik
Translated Amino Acid sequence (All Frames) 
Frame A:
LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILPV---

---LKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAA
SEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKL
TASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGV
MVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGENHNYAXLT
FRKF*fys*yvik

Frame B:
yyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkrmvlfhhselqfhqv
hqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvp
qikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvi
n*lcknl*fyql---

---*rmssvvdtvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlp
lnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*
qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvs
wlaivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vktitmlv*l
svnfnfthnm*sk

Frame C:
itlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkgwccfiiqscssirc
inwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwyp
k*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**
isyariydftsw---

---kechqw*irsrcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl
*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*in
sis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgch
g*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*kp*lcxfnf
p*ililliicnqn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       2551   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1471   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1469   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1467   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1463   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1461   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1459   0.0
CHB226 (CHB226Q) /CSM/CH/CHB2-B/CHB226Q.Seq.d/                       1459   0.0
AFG692 (AFG692Q) /CSM/AF/AFG6-D/AFG692Q.Seq.d/                       1459   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1453   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X80474|X80474.1 N.frontalis enolase gene.                          64   3e-76  13
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   2e-72  11
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-60  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-60  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   2e-60  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   1e-58  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   5e-52  5
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121   2e-48  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66   5e-46  6
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   2e-45  10
dna update 2003.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   801   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   578   e-163
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   567   e-160
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   559   e-158
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   558   e-157
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   557   e-157
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   557   e-157
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   557   e-157
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   556   e-157
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   555   e-156
protein update 2009. 5.22
PSORT 

 psg: 0.95  gvh: 0.47  alm: 0.32  top: 0.60  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 16.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for SFB846 is mit
5' end seq. ID SFB846F
5' end seq. 
>SFB846F.Seq
TTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTC
ATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTT
GACCTTTACACCGAAAAGGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGT
GCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTA
GGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATT
GGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACC
CCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGA
GCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACT
AAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAAT
AAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGG----------
Length of 5' end seq. 578
3' end seq. ID SFB846Z
3' end seq. 
>SFB846Z.Seq
----------TTAAAGAATGTCATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGG
TGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAA
ACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGC
CTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGG
TTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATA
TCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATT
AACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAG
AATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAAT
TGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGT
CATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGG
TTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATA
CAATCAACTTGTTAGAATTAACGAAGAGTTAGGTGAAAACCATAACTATGCTNGTTTAAC
TTTCCGTAAATTTTAATTTTACTCATAATATGTAATCAAAAC
Length of 3' end seq. 752
Connected seq. ID SFB846P
Connected seq. 
>SFB846P.Seq
TTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTC
ATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTT
GACCTTTACACCGAAAAGGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGT
GCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTA
GGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATT
GGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACC
CCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGA
GCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACT
AAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAAT
AAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGG----------TTAAAGAATGTC
ATCAGTGGTAGATACGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCA
CCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGT
TACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAAT
GGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAA
AAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGAT
CCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAA
ATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAG
AAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATT
CGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGT
GAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAA
ACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAAC
GAAGAGTTAGGTGAAAACCATAACTATGCTNGTTTAACTTTCCGTAAATTTTAATTTTAC
TCATAATATGTAATCAAAAC
Length of connected seq. 1330
Full length Seq ID -
Full length Seq. -
Length of full length seq. -