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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH843
Library AF
(Link to library)
Clone ID AFH843
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16368-1
Original site URL
Representative seq. ID AFH843P
(Link to Original site)
Representative DNA sequence 
>AFH843 (AFH843Q) /CSM/AF/AFH8-B/AFH843Q.Seq.d/
AAATAATTATATATATATATAAAAATNTAAAAAAAATAATAATGAGAATTTTATATTTAG
CTTCTCTTTTATTTTTAATTACATTATATCTATCACCAACTTTTGGNTGGGGTGGTGGCA
GAGATTGNGAANGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATT
TACCATTTGTATCATGTTGNGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAA
ATTGTTGTGATGATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTN
GGTCAAGTTGTAAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACAT
GTGAATCATTAGGTTGTGAAGCAAGAGGTNTGGAATGTGAATGGGTTGAATCATCACCAT
GTTATGGAACTTCTTGTTGCCCAAGAAXXXXXXXXXXTTCACCAAAGAATGACGAATGTA
AATGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCAATAGTAGAAACGGTCCACACTGTC
TTGCCAATAATGTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTT
GTAAAATGATTAGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCAC
ATTGCTCAACTTGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTC
AAGTACCAAGCAATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTA
TTCATCCATCAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
IIIYIYKNXKKIIMRILYLASLLFLITLYLSPTFGWGGGRDXEXHRSEYTCKSDRSCAYL
PFVSCXGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIXSSCKPHRDFVPYHSPNTTTC
ESLGCEARGXECEWVESSPCYGTSCCPR---

---SPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCKMIRGNPTC
IKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPS
Translated Amino Acid sequence (All Frames) 
Frame A:
k*lyiyikx*kk***efyi*llfyf*lhyiyhqllxgvvaeixxvivqnihvnqievvhi
yhlyhvxvrknfsvliviieivvmiyhvlktrelvkslrxgqvvnhieilyhiihqiqph
vnh*vvkqevwnvnglnhhhvmellvaqe---

---ftke*rm*maslstrfqllq**krstlscq*cipttl*sysmsn*fll*nd*r*snl
y*skttgttttllnlcraiism*srwndlysstkqlyqy*ipmlpissnlyssi

Frame B:
nnyiyi*kxkknnnenfifsfsfifnyiisitnfwxgwwqrlxxssfriym*ir*klcif
ticimlx*eriflc*s*s*kll**fimc*khenw*nl*dxvkl*tt*rfctisftkynhm
*iirl*skrxgm*mg*iitmlwnfllpk---

---SPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCKMIRGNPTC
IKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPS

Frame C:
IIIYIYKNXKKIIMRILYLASLLFLITLYLSPTFGWGGGRDXEXHRSEYTCKSDRSCAYL
PFVSCXGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIXSSCKPHRDFVPYHSPNTTTC
ESLGCEARGXECEWVESSPCYGTSCCPR---

---hqrmtnvngivvhqvpaasivetvhtvlpimyshnfvkllnvqlislvk*leviqlv
lkqdhryhhhiaqlvqsyhqhvitle*fvfkyqaivpildshvahliqfvfihq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSA149 (SSA149Q) /CSM/SS/SSA1-C/SSA149Q.Seq.d/                        765   0.0
SLG851 (SLG851Q) /CSM/SL/SLG8-C/SLG851Q.Seq.d/                        765   0.0
SLB538 (SLB538Q) /CSM/SL/SLB5-B/SLB538Q.Seq.d/                        765   0.0
SLA438 (SLA438Q) /CSM/SL/SLA4-B/SLA438Q.Seq.d/                        765   0.0
SLA432 (SLA432Q) /CSM/SL/SLA4-B/SLA432Q.Seq.d/                        765   0.0
SLA402 (SLA402Q) /CSM/SL/SLA4-A/SLA402Q.Seq.d/                        765   0.0
SHL109 (SHL109Q) /CSM/SH/SHL1-A/SHL109Q.Seq.d/                        765   0.0
SHJ527 (SHJ527Q) /CSM/SH/SHJ5-B/SHJ527Q.Seq.d/                        765   0.0
SHH249 (SHH249Q) /CSM/SH/SHH2-C/SHH249Q.Seq.d/                        765   0.0
SHF542 (SHF542Q) /CSM/SH/SHF5-B/SHF542Q.Seq.d/                        765   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.   664   0.0    3
BJ170760|BJ170760.1 Physcomitrella patens subsp. patens cDNA clone:pph26m16, 3' end, single read.   190   2e-44  1
AF279135|AF279135.1 Dictyostelium discoideum spore coat structural protein SP65 (cotE) gene, complete cds.    64   3e-06  1
BX511123|BX511123.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    46   0.010  5
AC084865|AC084865.3 Homo sapiens chromosome 7 clone RP11-437L1, complete sequence.    48   0.21   1
AC079746|AC079746.2 Homo sapiens chromosome 7 clone CTD-2353O2, WORKING DRAFT SEQUENCE, 19 unordered pieces.    48   0.21   1
AC073320|AC073320.8 Homo sapiens clone RP11-306G20, complete sequence.    48   0.21   1
AZ208391|AZ208391.1 SP_0150_A1_B06_T7A Strongylocentrotus purpuratus, purple sea urchin , sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=150 Col=11 Row=C, DNA sequence.    48   0.21   1
AC145891|AC145891.4 Pan troglodytes BAC clone RP43-20A24 from 7, complete sequence.    48   0.21   1
AL035475|AL035475.7 Plasmodium falciparum MAL4P2.                  36   0.28   8
dna update 2004. 6.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       313   4e-84
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   306   6e-82
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   131   2e-29
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    77   5e-13
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    61   4e-08
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    61   4e-08
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    58   3e-07
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    55   3e-06
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    54   4e-06
AP008215_740(AP008215|pid:none) Oryza sativa (japonica cultivar-...    52   2e-05
protein update 2009. 4.22
PSORT 

 psg: 0.81  gvh: 0.77  alm: 0.24  top: 0.17  tms: 0.07  mit: 0.33  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.83  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for AFH843 is cyt
5' end seq. ID AFH843F
5' end seq. 
>AFH843F.Seq
AAATAATTATATATATATATAAAAATNTAAAAAAAATAATAATGAGAATTTTATATTTAG
CTTCTCTTTTATTTTTAATTACATTATATCTATCACCAACTTTTGGNTGGGGTGGTGGCA
GAGATTGNGAANGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATT
TACCATTTGTATCATGTTGNGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAA
ATTGTTGTGATGATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTN
GGTCAAGTTGTAAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACAT
GTGAATCATTAGGTTGTGAAGCAAGAGGTNTGGAATGTGAATGGGTTGAATCATCACCAT
GTTATGGAACTTCTTGTTGCCCAAGAA----------
Length of 5' end seq. 447
3' end seq. ID AFH843Z
3' end seq. 
>AFH843Z.Seq
----------TTCACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCAGGTTCCA
GCTGCTTCAATAGTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTTT
GTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACTT
GTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCAT
CAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTA
GATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAA
Length of 3' end seq. 335
Connected seq. ID AFH843P
Connected seq. 
>AFH843P.Seq
AAATAATTATATATATATATAAAAATNTAAAAAAAATAATAATGAGAATTTTATATTTAG
CTTCTCTTTTATTTTTAATTACATTATATCTATCACCAACTTTTGGNTGGGGTGGTGGCA
GAGATTGNGAANGTCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATT
TACCATTTGTATCATGTTGNGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAA
ATTGTTGTGATGATTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTN
GGTCAAGTTGTAAACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACAT
GTGAATCATTAGGTTGTGAAGCAAGAGGTNTGGAATGTGAATGGGTTGAATCATCACCAT
GTTATGGAACTTCTTGTTGCCCAAGAA----------TTCACCAAAGAATGACGAATGTA
AATGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCAATAGTAGAAACGGTCCACACTGTC
TTGCCAATAATGTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTT
GTAAAATGATTAGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCAC
ATTGCTCAACTTGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTC
AAGTACCAAGCAATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTA
TTCATCCATCAA
Length of connected seq. 782
Full length Seq ID -
Full length Seq. -
Length of full length seq. -