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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFB745
Library SF
(Link to library)
Clone ID SFB745
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID SFB745P
(Link to Original site)
Representative DNA sequence 
>SFB745 (SFB745Q) /CSM/SF/SFB7-B/SFB745Q.Seq.d/
ATTGTTATAATCAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACAT
TAACATTGTCGTCATTGGTCACGCAGATGCTGGTAAATCAACCACCACTGGTCATTTAAT
CTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGA
AATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGA
AAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCAC
CATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCA
AGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGC
CAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAAT
GATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGA
CGAAATTGTCAAGGAAGXXXXXXXXXXTCCACACGATAAACCATTACGTATTCCATTACA
AGATGTCTACAAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGCCGAAACTGGTAT
CATTAAACCAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATC
AGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAA
CGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAA
CGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGG
TCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAA
ATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGG
TACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCG
TCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGA
TATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAA
AGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTC
TTAATAAATAGCATTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ll*stlkke*TTMGKEKTHINIVVIGHADAGKSTTTGHLIYKCGGIDKRVIEKYEKEASE
MGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQ
ADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYD
EIVKE---

---PHDKPLRIPLQDVYKIGGIGTVPVGRAETGIIKPGMVVTFAPAGLSTEVKSVEMHHE
QLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGY
SPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVES
FTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iaf
Translated Amino Acid sequence (All Frames) 
Frame A:
iviinfkerinnhg*rensh*hcrhwsrrcw*inhhwsfnlqmwwyr*esyrkv*kgsf*
ng*tilqirlgyg*ikg*t*kryhhrycfmeirnlqillhhh*cprsqrfhqeydywylp
srlcrlshclpnw*irswyrqkrsns*trspclhfrcqtndrcyqqdg*kinqlltsplr
rncqg---

---str*titysitrclqdrwyrycssrscrnwyh*trygrhlcpswsln*sqisrnasr
ttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnpcwl
ltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*i
lh*iptirsfrrqryetnrrrrchqinrqespr*sr**ercccpikkeink*yflinsi

Frame B:
ll*stlkke*TTMGKEKTHINIVVIGHADAGKSTTTGHLIYKCGGIDKRVIEKYEKEASE
MGKQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQ
ADCAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIVAINKMDEKSTNYSQARYD
EIVKE---

---PHDKPLRIPLQDVYKIGGIGTVPVGRAETGIIKPGMVVTFAPAGLSTEVKSVEMHHE
QLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIHAGY
SPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMCVES
FTEYPPLGRFAVRDMRQTVAVGVIKSTVKKAPGKAGDKKGAAAPSKKK*innis**iaf

Frame C:
cynql*rknkqpwvkrkltltlsslvtqmlvnqpplvi*stnvvvsirelsksmkrkllk
wvnnpsntpglwin*rlnvkevspsillygnskppnttspslmpqvteissri*llvppk
pivps*slppqlvnsklvspktvklvntlslltl*vsnk*sllstrwmknqptthkpvtt
klsrk---

---htinhyvfhykmstrsvvsvlfq*vvpklvslnqvwssplpqlvsqlksnqskcitn
nsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvksmlvt
hqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmplwsn*phlvqcvlnp
slnthh*vvspsei*dkpspsvssnqpsrkpqvkpvirkvllphqkrnk*iiflnk*h
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFB745 (SFB745Q) /CSM/SF/SFB7-B/SFB745Q.Seq.d/                       2470   0.0
VFN174 (VFN174Q) /CSM/VF/VFN1-D/VFN174Q.Seq.d/                       1398   0.0
VFM606 (VFM606Q) /CSM/VF/VFM6-A/VFM606Q.Seq.d/                       1398   0.0
VFM163 (VFM163Q) /CSM/VF/VFM1-C/VFM163Q.Seq.d/                       1398   0.0
VFI880 (VFI880Q) /CSM/VF/VFI8-D/VFI880Q.Seq.d/                       1398   0.0
VFI822 (VFI822Q) /CSM/VF/VFI8-A/VFI822Q.Seq.d/                       1398   0.0
VFI781 (VFI781Q) /CSM/VF/VFI7-D/VFI781Q.Seq.d/                       1398   0.0
VFI708 (VFI708Q) /CSM/VF/VFI7-A/VFI708Q.Seq.d/                       1398   0.0
VFI478 (VFI478Q) /CSM/VF/VFI4-D/VFI478Q.Seq.d/                       1398   0.0
VFH853 (VFH853Q) /CSM/VF/VFH8-C/VFH853Q.Seq.d/                       1398   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1312   0.0    1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1283   0.0    1
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.  1084   0.0    2
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   256   0.0    8
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   192   0.0    9
AX109715|AX109715.1 Sequence 448 from Patent WO0123604.           139   e-159  9
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.           139   e-158  9
AF402092|AF402092.1 Pichia anomala strain NRRL Y-366 translation elongation factor 1-alpha gene, partial cds.   143   e-158  9
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.           161   e-148  8
AX109682|AX109682.1 Sequence 415 from Patent WO0123604.           161   e-147  8
dna update 2003.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FJ807244_1(FJ807244|pid:none) Reclinomonas americana elongation ...   572   e-162
AY643820_1(AY643820|pid:none) Arcyria denudata elongation factor...   567   e-160
FJ807246_1(FJ807246|pid:none) Stachyamoeba lipophora elongation ...   566   e-160
DQ138080_1(DQ138080|pid:none) Phytophthora parasitica isolate 14...   565   e-159
AY785728_1(AY785728|pid:none) Trypanosoma rangeli strain San Agu...   564   e-159
AY785665_1(AY785665|pid:none) Trypanosoma cruzi strain M6241 cl6...   563   e-159
AY785676_1(AY785676|pid:none) Trypanosoma cruzi strain NR cl3 cl...   563   e-159
AY785660_1(AY785660|pid:none) Trypanosoma cruzi strain Dm 28 c c...   563   e-159
AY785657_1(AY785657|pid:none) Trypanosoma cruzi strain Colombian...   563   e-159
FJ807243_1(FJ807243|pid:none) Peranema trichophorum strain CCAP ...   563   e-159
protein update 2009. 5.22
PSORT 

 psg: 0.84  gvh: 0.35  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SFB745 is nuc
5' end seq. ID SFB745F
5' end seq. 
>SFB745F.Seq
ATTGTTATAATCAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACAT
TAACATTGTCGTCATTGGTCACGCAGATGCTGGTAAATCAACCACCACTGGTCATTTAAT
CTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGA
AATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGA
AAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCAC
CATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCA
AGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGC
CAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAAT
GATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGA
CGAAATTGTCAAGGAAG----------
Length of 5' end seq. 557
3' end seq. ID SFB745Z
3' end seq. 
>SFB745Z.Seq
----------TCCACACGATAAACCATTACGTATTCCATTACAAGATGTCTACAAGATCG
GTGGTATCGGTACTGTTCCAGTAGGTCGTGCCGAAACTGGTATCATTAAACCAGGTATGG
TCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACG
AACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAG
TCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAA
CTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTT
ACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCG
ATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCT
TAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAAT
CCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCG
CCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAG
GTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTT
Length of 3' end seq. 709
Connected seq. ID SFB745P
Connected seq. 
>SFB745P.Seq
ATTGTTATAATCAACTTTAAAGAAAGAATAAACAACCATGGGTAAAGAGAAAACTCACAT
TAACATTGTCGTCATTGGTCACGCAGATGCTGGTAAATCAACCACCACTGGTCATTTAAT
CTACAAATGTGGTGGTATCGATAAGAGAGTTATCGAAAAGTATGAAAAGGAAGCTTCTGA
AATGGGTAAACAATCCTTCAAATACGCCTGGGTTATGGATAAATTAAAGGCTGAACGTGA
AAGAGGTATCACCATCGATATTGCTTTATGGAAATTCGAAACCTCCAAATACTACTTCAC
CATCATTGATGCCCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCCCA
AGCCGATTGTGCCGTCTTAGTCATTGCCTCCCCAACTGGTGAATTCGAAGCTGGTATCGC
CAAAAACGGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAAT
GATCGTTGCTATCAACAAGATGGATGAAAAATCAACCAACTACTCACAAGCCCGTTACGA
CGAAATTGTCAAGGAAG----------TCCACACGATAAACCATTACGTATTCCATTACA
AGATGTCTACAAGATCGGTGGTATCGGTACTGTTCCAGTAGGTCGTGCCGAAACTGGTAT
CATTAAACCAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATC
AGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAA
CGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAA
CGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGG
TCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAA
ATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGG
TACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCG
TCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGA
TATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAA
AGCCGGTGATAAGAAAGGTGCTGCTGCCCCATCAAAAAAGAAATAAATAAATAATATTTC
TTAATAAATAGCATTT
Length of connected seq. 1266
Full length Seq ID -
Full length Seq. -
Length of full length seq. -