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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFB722
Library SF
(Link to library)
Clone ID SFB722
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SFB722P
(Link to Original site)
Representative DNA sequence 
>SFB722 (SFB722Q) /CSM/SF/SFB7-A/SFB722Q.Seq.d/
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAG
CCTATCGTATGGGTTCAGAAGXXXXXXXXXXTCGTATGGGTTCAGAAGTTTATCATAACT
TAAAGAATGTCATCAGTGGTAGATACGGGTCAAGATGCTATCAATGTTGGTGATGAAGGT
GGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATT
GAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTC
AAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTT
ATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATT
TCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCA
GTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACT
GGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTC
ACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGC
CATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACT
GGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTT
GTTAGAATTAACGAAGGGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAA
TTTTAATTTTACTCATAATATG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILPVGAKDFNEAYRMGSE---

---RMGSEVYHNLKNVISGRYGSRCYQCW**rwfrstnskqqrrs*tlktch*kswlhrf
s*nwyglccl*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*s
r*lgiih*insis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs
*lkecimgchg*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr
*kp*lcwfnfp*ilillii
Translated Amino Acid sequence (All Frames) 
Frame A:
tyyfiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhq
vhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismv
pqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqv
in*lcknl*fyqlvlkismkpivwvqk---

---sygfrsls*lkechqw*irvkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv
*lklvwivlplnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlkihli
kmignhtln*qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvpl
ltqrmhhgvswlaivqvklkipslli*llv*vlvklklvlhvdqkd*pntinlleltkg*
vktitmlv*lsvnfnfthnm

Frame B:
litlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssir
cinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwy
pk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr*
*isyariydftswc*rfq*slsygfr---

---RMGSEVYHNLKNVISGRYGSRCYQCW**rwfrstnskqqrrs*tlktch*kswlhrf
s*nwyglccl*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*s
r*lgiih*insis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs
*lkecimgchg*psfr*n*rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr
*kp*lcwfnfp*ilillii

Frame C:
LLLYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSG
ASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGT
PNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGN
KLAMQEFMILPVGAKDFNEAYRMGSE---

---vwvqkfiit*rmssvvdtgqdainvgdeggfappiqsnkeglellklaiekagytgl
vkigmdcaasefkvengydldfktknndgsavisgeklgdlyrefikeypiisiedpfdq
ddwesytkltasvdiqivgddllvtnperiktgiekkacnalllkvnqigsvtesiraal
dsknaswgvmvshrsgetedtfiadlvvglgtgqiktgapcrserlakynqlvrineglg
enhnyagltfrkf*fys*y
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFB722 (SFB722Q) /CSM/SF/SFB7-A/SFB722Q.Seq.d/                       2676   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1493   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1491   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1473   0.0
AFC510 (AFC510Q) /CSM/AF/AFC5-A/AFC510Q.Seq.d/                       1465   0.0
SFC169 (SFC169Q) /CSM/SF/SFC1-C/SFC169Q.Seq.d/                       1451   0.0
AFC723 (AFC723Q) /CSM/AF/AFC7-A/AFC723Q.Seq.d/                       1447   0.0
SFB846 (SFB846Q) /CSM/SF/SFB8-B/SFB846Q.Seq.d/                       1443   0.0
AFK881 (AFK881Q) /CSM/AF/AFK8-D/AFK881Q.Seq.d/                       1439   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1437   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   2e-75  12
X80474|X80474.1 N.frontalis enolase gene.                          64   5e-74  12
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-62  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   1e-62  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   1e-62  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   1e-60  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   1e-53  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66   2e-51  7
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121   3e-48  5
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   7e-47  10
dna update 2003.12.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   461   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   342   e-166
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   335   e-163
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   325   e-161
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   341   e-161
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   332   e-160
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   321   e-160
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   320   e-160
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   339   e-160
(Q43130) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   338   e-160
protein update 2009. 5.22
PSORT 

 psg: 0.95  gvh: 0.47  alm: 0.32  top: 0.60  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for SFB722 is cyt
5' end seq. ID SFB722F
5' end seq. 
>SFB722F.Seq
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAG
CCTATCGTATGGGTTCAGAAG----------
Length of 5' end seq. 621
3' end seq. ID SFB722Z
3' end seq. 
>SFB722Z.Seq
----------TCGTATGGGTTCAGAAGTTTATCATAACTTAAAGAATGTCATCAGTGGTA
GATACGGGTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATTCAA
AGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACAGGT
TTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTACGAT
CTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTAGGT
GATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTTGAT
CAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTTGGT
GATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCATGT
AATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCCGCT
CTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACTGAA
GATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGTGCT
CCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGGGTTA
GGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAATTTTAATTTTACTCATAATAT
G
Length of 3' end seq. 771
Connected seq. ID SFB722P
Connected seq. 
>SFB722P.Seq
ACTTATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCG
TCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAG
TTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAG
GTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACT
TAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCA
TTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTA
CCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCA
GAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTA
CTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTA
ATAAATTAGCTATGCAAGAATTTATGATTTTACCAGTTGGTGCTAAAGATTTCAATGAAG
CCTATCGTATGGGTTCAGAAG----------TCGTATGGGTTCAGAAGTTTATCATAACT
TAAAGAATGTCATCAGTGGTAGATACGGGTCAAGATGCTATCAATGTTGGTGATGAAGGT
GGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATT
GAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTC
AAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTT
ATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATT
TCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCA
GTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACT
GGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTC
ACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGC
CATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACT
GGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTT
GTTAGAATTAACGAAGGGTTAGGTGAAAACCATAACTATGCTGGTTTAACTTTCCGTAAA
TTTTAATTTTACTCATAATATG
Length of connected seq. 1392
Full length Seq ID -
Full length Seq. -
Length of full length seq. -