>AFH828 (AFH828Q) /CSM/AF/AFH8-B/AFH828Q.Seq.d/
XXXXXXXXXXAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATAT
GAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCAT
CTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTC
ATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGT
TAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCAC
TTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATG
GTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGA
CGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATAT
CGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAAT
CTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGC
TGCCATTGAATTCAGAATCACCATCATCTCAACCTGATGATAATGNGNAGTCNAAAAAAG
ATTCTTCTGCTAA

---IATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSF
DTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWC
GHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPIS
YEGQRNDHMNFVEFIQDNAAIEFRITIIST***xxvxkrffc*

Frame A:
---kslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkkpsh
sipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlhg
aviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiqs
lmkvkemii*tllnsfkitlplnsespssqpddnxxskkdssa


Frame B:
---nryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnlli
ryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrsmv
rslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrksnl
l*rskk*syelc*ihsr*rch*iqnhhhlnlmimxsxkkilll


Frame C:
---IATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETFSF
DTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAPWC
GHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENPIS
YEGQRNDHMNFVEFIQDNAAIEFRITIIST***xxvxkrffc*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH828 (AFH828Q) /CSM/AF/AFH8-B/AFH828Q.Seq.d/                       1261   0.0
AFO770 (AFO770Q) /CSM/AF/AFO7-C/AFO770Q.Seq.d/                       1229   0.0
AFN113 (AFN113Q) /CSM/AF/AFN1-A/AFN113Q.Seq.d/                       1229   0.0
SFL246 (SFL246Q) /CSM/SF/SFL2-B/SFL246Q.Seq.d/                       1227   0.0
SFK855 (SFK855Q) /CSM/SF/SFK8-C/SFK855Q.Seq.d/                       1227   0.0
SFI119 (SFI119Q) /CSM/SF/SFI1-A/SFI119Q.Seq.d/                       1227   0.0
AHO764 (AHO764Q) /CSM/AH/AHO7-C/AHO764Q.Seq.d/                       1227   0.0
AFO829 (AFO829Q) /CSM/AF/AFO8-B/AFO829Q.Seq.d/                       1227   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1227   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1227   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   5e-08  1
BC067155|BC067155.1 Danio rerio zgc:77086, mRNA (cDNA clone MGC:77086 IMAGE:6960343), complete cds.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   171   2e-41
(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   167   2e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   166   4e-40
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   166   7e-40
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   161   1e-38
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   159   9e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   153   4e-36
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   2e-35

 psg: 0.86  gvh: 0.36  alm: 0.43  top: 0.50  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFH828 is cyt

>AFH828Z.Seq
----------AAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATAT
GAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCAT
CTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTC
ATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGT
TAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCAC
TTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATG
GTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGA
CGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATAT
CGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAAT
CTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGC
TGCCATTGAATTCAGAATCACCATCATCTCAACCTGATGATAATGNGNAGTCNAAAAAAG
ATTCTTCTGCTAA