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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFB251
Library SF
(Link to library)
Clone ID SFB251
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11649-1|Contig-U16366-1
Original site URL
Representative seq. ID SFB251P
(Link to Original site)
Representative DNA sequence 
>SFB251 (SFB251Q) /CSM/SF/SFB2-C/SFB251Q.Seq.d/
AGATGGCTGTTAAATACCATCCAGATAAAAATCAAGGACCAGGTAAAGATGCAGCCGAAG
CAAAATTTAAAGATATTAGTGAAGCATATGAAGTTTTAAGTGATCCAGAGAAGAGAAAGA
TGTACGATAGTTATGGTTCTGAAGGTATGAAGGAGAGTGGTTTCCATGCATCATCAGCTG
AAGACCTCTTCTCACATTTCTTTGGTGCAGGAGGTGGCGGTGGTGGATTTAGTTTCGGAG
GTGGTGGTGGTGATGATTTTGGTGGATTTAGTTTTGGTAATATGGGAGGTATGGGCGGCA
TGGGTGGCATGGGTGGNGGNCACANGANGAGAAGANNGGGNGANGATNTNGNNCATGANN
NGANTNGANNNNNGNNNGANNNNTNTNNNGNNNNNNNAXXXXXXXXXXGGTAAGAAGATC
GGTACAATTCCAAGTCGAGAAAGGTACTAGAGATGGCGAAAGAATCATGTTACAAGGTCA
AGGTTCCGAGTATCCAGGTGTACCACCAGGTGATGTAATCATTACAATTCGTGAGAAACC
AAAATGTAAATTTCAAGAGAAATGGTGATAATTTAATCTACACCAAACGTTTAAAATTAT
TAGACTCAATCGCTGGTAGTCAATTCATTATAAACACATTGGATCAACGTAAACTTTGGG
TCAATCATGAGAAAGGTGATATAATTAAGCAAGGTGATATGCGTTACATAGAGAATGAAG
GTATGCCAATTAAAGGTACCTCTAGAAAAGGTAAATTAATCATTGCTTTTGATATTGAAT
ATCCATCGAATCTTACAAATGATGATATTGAAAAACTCTCTAAAATCTTACCAAAAGCTG
CCACCCCTTCTGTTTCAAAATCTGATTGTAAATCGGTTGGTCTCTCAAAGGTAAACTTTA
ATACCAACGAACAAAGTTCTCATGGTGGTGCTGGTGGTGCTTATCAACAACATGGTGGTG
CTTATGGTCATCAAAAACAACAACAACAAGGTTTTAATCCTGCTNATTTTGGTGCTCAAT
TTGGTGGTGGGGTCCACAACAAGCACAACAGTGTCAACAACAATAAACAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
MAVKYHPDKNQGPGKDAAEAKFKDISEAYEVLSDPEKRKMYDSYGSEGMKESGFHASSAE
DLFSHFFGAGGGGGGFSFGGGGGDDFGGFSFGNMGGMGGMGGMGGGHXXRRXGXDXXHXX
XXXXXXXXXXXX---

---VRRSVQFQVEKGTRDGERIMLQGQGSEYPGVPPGDVIITIREKPKCKFQEKW**fnl
hqtfkiirlnrw*sihykhigst*tlgqs*er*yn*ar*yalhre*ryan*ryl*kr*in
hcf*y*isiesyk**y*ktl*nltkschpfcfki*l*igwslkgkl*yqrtkfswwcwwc
lsttwwclwsskttttrf*scxfwcsiwwwgpqqaqqcqqq*t
Translated Amino Acid sequence (All Frames) 
Frame A:
rwllntiqikikdqvkmqpkqnlkilvkhmkf*viqrrerctivmvlkv*rrvvsmhhql
ktsshislvqevavvdlvsevvvvmilvdlvlviwevwaawvawvxxtxxexxxxxxxmx
xxxxxxxxxxxx---

---gkkigtipsrery*rwrknhvtrsrfrvsrcttr*cnhyns*etkm*isremvii*s
tpnv*ny*tqslvvnsl*thwinvnfgsimrkvi*lskvicvt*rmkvcqlkvplekvn*
slllilnihrilqmmilknslksyqklppllfqnlivnrlvsqr*tliptnkvlmvvlvv
linnmvvlmviknnnnkvlillilvlnlvvgsttsttvsttin

Frame B:
dgc*ipsr*ksrtr*rcsrski*ry**si*sfk*sreekdvr*lwf*ryegewfpciis*
rplltflwcrrwrwwi*frrwww**fwwi*fw*ygrygrhgwhgwxxxxekxgxxxxx*x
xxxxxxxxxxxx---

---VRRSVQFQVEKGTRDGERIMLQGQGSEYPGVPPGDVIITIREKPKCKFQEKW**fnl
hqtfkiirlnrw*sihykhigst*tlgqs*er*yn*ar*yalhre*ryan*ryl*kr*in
hcf*y*isiesyk**y*ktl*nltkschpfcfki*l*igwslkgkl*yqrtkfswwcwwc
lsttwwclwsskttttrf*scxfwcsiwwwgpqqaqqcqqq*t

Frame C:
MAVKYHPDKNQGPGKDAAEAKFKDISEAYEVLSDPEKRKMYDSYGSEGMKESGFHASSAE
DLFSHFFGAGGGGGGFSFGGGGGDDFGGFSFGNMGGMGGMGGMGGGHXXRRXGXDXXHXX
XXXXXXXXXXXX---

---*edrynsksrkvlemakescykvkvpsiqvyhqvm*slqfvrnqnvnfkrngdnliy
tkrlklldsiagsqfiintldqrklwvnhekgdiikqgdmryienegmpikgtsrkgkli
iafdieypsnltnddieklskilpkaatpsvsksdcksvglskvnfntneqsshggagga
yqqhggayghqkqqqqgfnpaxfgaqfgggvhnkhnsvnnnkq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFB251 (SFB251Q) /CSM/SF/SFB2-C/SFB251Q.Seq.d/                       1306   0.0
CHC270 (CHC270Q) /CSM/CH/CHC2-C/CHC270Q.Seq.d/                       1275   0.0
SLH333 (SLH333Q) /CSM/SL/SLH3-B/SLH333Q.Seq.d/                       1255   0.0
SFE772 (SFE772Q) /CSM/SF/SFE7-C/SFE772Q.Seq.d/                       1255   0.0
SLA810 (SLA810Q) /CSM/SL/SLA8-A/SLA810Q.Seq.d/                       1245   0.0
CFJ662 (CFJ662Q) /CSM/CF/CFJ6-C/CFJ662Q.Seq.d/                       1245   0.0
SLE413 (SLE413Q) /CSM/SL/SLE4-A/SLE413Q.Seq.d/                       1225   0.0
AFK111 (AFK111Q) /CSM/AF/AFK1-A/AFK111Q.Seq.d/                       1199   0.0
CFG324 (CFG324Q) /CSM/CF/CFG3-A/CFG324Q.Seq.d/                       1191   0.0
AFL309 (AFL309Q) /CSM/AF/AFL3-A/AFL309Q.Seq.d/                       1166   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116963|AC116963.1 Dictyostelium discoideum chromosome 2 map 4790445-4846595 strain AX4, *** SEQUENCING IN PROGRESS ***.    40   2e-06  9
CK245502|CK245502.1 EST729139 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAE75 5' end, mRNA sequence.    62   2e-06  2
BP505917|BP505917.1 Hydra magnipapillata cDNA, clone:hm_03959.     60   2e-05  2
CF600357|CF600357.1 tac28g11.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to TR:O60884 O60884 DNAJ PROTEIN. ;, mRNA sequence.    60   2e-05  2
CA301645|CA301645.1 taa06b03.y1 Hydra cDNA library Hydra magnipapillata cDNA 5' similar to TR:O60884 O60884 DNAJ PROTEIN. ;, mRNA sequence.    60   2e-05  2
CK161208|CK161208.1 FGAS013773 Triticum aestivum FGAS: Library 4 Gate 8 Triticum aestivum cDNA, mRNA sequence.    56   3e-05  2
BF595059|BF595059.1 su63e08.y1 Gm-c1069 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1069-1744 5' similar to TR:O24074 O24074 DNAJ-LIKE PROTEIN. ;, mRNA sequence.    58   4e-05  2
BE805892|BE805892.1 ss62f10.y1 Gm-c1062 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1062-1028 5' similar to TR:O24074 O24074 DNAJ-LIKE PROTEIN. ;, mRNA sequence.    58   4e-05  2
BM309208|BM309208.1 sak55h09.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-7265 5' similar to TR:O24074 O24074 DNAJ-LIKE PROTEIN. ;, mRNA sequence.    58   4e-05  2
BG881859|BG881859.1 sae90f10.y1 Gm-c1065 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1065-3332 5' similar to TR:O24074 O24074 DNAJ-LIKE PROTEIN. ;, mRNA sequence.    58   4e-05  2
dna update 2003.12.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54ED3) RecName: Full=DnaJ homolog subfamily A member 1 homolog;     258   3e-87
AB264719_1(AB264719|pid:none) Papilio xuthus mRNA for similar to...    76   7e-21
EU306561_1(EU306561|pid:none) Tigriopus japonicus heat shock pro...    77   2e-20
AY267904_1(AY267904|pid:none) Sus scrofa pDJA1 chaperone mRNA, c...    72   2e-19
BC046660_1(BC046660|pid:none) Xenopus laevis similar to DnaJ (Hs...    77   3e-19
AK016666_1(AK016666|pid:none) Mus musculus adult male testis cDN...    71   3e-19
(P25491) RecName: Full=Mitochondrial protein import protein MAS5...    73   5e-19
BC082010_1(BC082010|pid:none) Rattus norvegicus DnaJ (Hsp40) hom...    70   1e-18
(Q95JF4) RecName: Full=DnaJ homolog subfamily A member 1; AltNam...    72   1e-18
(P63036) RecName: Full=DnaJ homolog subfamily A member 1; AltNam...    71   1e-18
protein update 2009. 5.22
PSORT 

 psg: 0.51  gvh: 0.30  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for SFB251 is cyt
5' end seq. ID SFB251F
5' end seq. 
>SFB251F.Seq
AGATGGCTGTTAAATACCATCCAGATAAAAATCAAGGACCAGGTAAAGATGCAGCCGAAG
CAAAATTTAAAGATATTAGTGAAGCATATGAAGTTTTAAGTGATCCAGAGAAGAGAAAGA
TGTACGATAGTTATGGTTCTGAAGGTATGAAGGAGAGTGGTTTCCATGCATCATCAGCTG
AAGACCTCTTCTCACATTTCTTTGGTGCAGGAGGTGGCGGTGGTGGATTTAGTTTCGGAG
GTGGTGGTGGTGATGATTTTGGTGGATTTAGTTTTGGTAATATGGGAGGTATGGGCGGCA
TGGGTGGCATGGGTGGNGGNCACANGANGAGAAGANNGGGNGANGATNTNGNNCATGANN
NGANTNGANNNNNGNNNGANNNNTNTNNNGNNNNNNNA----------
Length of 5' end seq. 398
3' end seq. ID SFB251Z
3' end seq. 
>SFB251Z.Seq
----------GGTAAGAAGATCGGTACAATTCCAAGTCGAGAAAGGTACTAGAGATGGCG
AAAGAATCATGTTACAAGGTCAAGGTTCCGAGTATCCAGGTGTACCACCAGGTGATGTAA
TCATTACAATTCGTGAGAAACCAAAATGTAAATTTCAAGAGAAATGGTGATAATTTAATC
TACACCAAACGTTTAAAATTATTAGACTCAATCGCTGGTAGTCAATTCATTATAAACACA
TTGGATCAACGTAAACTTTGGGTCAATCATGAGAAAGGTGATATAATTAAGCAAGGTGAT
ATGCGTTACATAGAGAATGAAGGTATGCCAATTAAAGGTACCTCTAGAAAAGGTAAATTA
ATCATTGCTTTTGATATTGAATATCCATCGAATCTTACAAATGATGATATTGAAAAACTC
TCTAAAATCTTACCAAAAGCTGCCACCCCTTCTGTTTCAAAATCTGATTGTAAATCGGTT
GGTCTCTCAAAGGTAAACTTTAATACCAACGAACAAAGTTCTCATGGTGGTGCTGGTGGT
GCTTATCAACAACATGGTGGTGCTTATGGTCATCAAAAACAACAACAACAAGGTTTTAAT
CCTGCTNATTTTGGTGCTCAATTTGGTGGTGGGGTCCACAACAAGCACAACAGTGTCAAC
AACAATAAACAA
Length of 3' end seq. 662
Connected seq. ID SFB251P
Connected seq. 
>SFB251P.Seq
AGATGGCTGTTAAATACCATCCAGATAAAAATCAAGGACCAGGTAAAGATGCAGCCGAAG
CAAAATTTAAAGATATTAGTGAAGCATATGAAGTTTTAAGTGATCCAGAGAAGAGAAAGA
TGTACGATAGTTATGGTTCTGAAGGTATGAAGGAGAGTGGTTTCCATGCATCATCAGCTG
AAGACCTCTTCTCACATTTCTTTGGTGCAGGAGGTGGCGGTGGTGGATTTAGTTTCGGAG
GTGGTGGTGGTGATGATTTTGGTGGATTTAGTTTTGGTAATATGGGAGGTATGGGCGGCA
TGGGTGGCATGGGTGGNGGNCACANGANGAGAAGANNGGGNGANGATNTNGNNCATGANN
NGANTNGANNNNNGNNNGANNNNTNTNNNGNNNNNNNA----------GGTAAGAAGATC
GGTACAATTCCAAGTCGAGAAAGGTACTAGAGATGGCGAAAGAATCATGTTACAAGGTCA
AGGTTCCGAGTATCCAGGTGTACCACCAGGTGATGTAATCATTACAATTCGTGAGAAACC
AAAATGTAAATTTCAAGAGAAATGGTGATAATTTAATCTACACCAAACGTTTAAAATTAT
TAGACTCAATCGCTGGTAGTCAATTCATTATAAACACATTGGATCAACGTAAACTTTGGG
TCAATCATGAGAAAGGTGATATAATTAAGCAAGGTGATATGCGTTACATAGAGAATGAAG
GTATGCCAATTAAAGGTACCTCTAGAAAAGGTAAATTAATCATTGCTTTTGATATTGAAT
ATCCATCGAATCTTACAAATGATGATATTGAAAAACTCTCTAAAATCTTACCAAAAGCTG
CCACCCCTTCTGTTTCAAAATCTGATTGTAAATCGGTTGGTCTCTCAAAGGTAAACTTTA
ATACCAACGAACAAAGTTCTCATGGTGGTGCTGGTGGTGCTTATCAACAACATGGTGGTG
CTTATGGTCATCAAAAACAACAACAACAAGGTTTTAATCCTGCTNATTTTGGTGCTCAAT
TTGGTGGTGGGGTCCACAACAAGCACAACAGTGTCAACAACAATAAACAA
Length of connected seq. 1060
Full length Seq ID -
Full length Seq. -
Length of full length seq. -