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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH810
Library AF
(Link to library)
Clone ID AFH810
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16368-1
Original site URL
Representative seq. ID AFH810P
(Link to Original site)
Representative DNA sequence 
>AFH810 (AFH810Q) /CSM/AF/AFH8-A/AFH810Q.Seq.d/
AAAAATTCTTTTTCTTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATAT
ATATATATAAAAATATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATT
TTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAG
TCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATC
ATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGA
TTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAA
ACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTGGG
TTGTGAAGCAAGAGGTATGGAATGTGAATGGGTNGAATCATCACCATGTTATGGAACTTC
TTGTTGCCCAAGAATTCCAAGATGTGTTGGNCXXXXXXXXXXGCACCAATATCGTGAAAT
GTTCACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCA
ATAGTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTTTGTAAAGTTA
CTCAATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACTTGTATTAAAG
CAAGACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCATCAGCATGTA
ATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGATTCCCAT
GTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCATTGCAA
CAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTG
GAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCG
CTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAA
GTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCT
CAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAGCTCAG
AAAGCTCAGAAAGCTCATCAGCCACTTCAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
kilflhsil*l*kkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCES
HRSEYTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCK
PHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVG---

---TNIVKCSPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCKMI
RGNPTCIKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPS
TTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSAAS
SAASSEPSSSAASSSAPSSASSSAPSSASSSAPSSSASSSAASSAASSESSESSSATS
Translated Amino Acid sequence (All Frames) 
Frame A:
knsfssfnfviikkgik*lyiyiki*kk*nenfifsfsfifnyiisitnfwlgwwqrl*k
ssfriym*ir*klcifticimlw*eriflc*s*s*kll**fimc*khenw*nl*dlvkl*
tt*rfctisftkynhm*iigl*skrygm*mgxiitmlwnfllpknskmcw---

---apis*nvhqrmtnvngivvhqvpaasivetvhtvlpimyshnfvkllnvqlislvk*
leviqlvlkqdhryhhhiaqlvqsyhqhvitle*fvfkyqaivpildshvahliqfvfih
qllllqplqqlhqlsqlqplqllqvqqlvelqlvvqllivvllpqpivalpphhhqavll
qvplqvnhqvaplqvvlhpllqvalhplpqvvlhhhqlqvqlhhqlqaqkaqkahqplh

Frame B:
kilflhsil*l*kkesnnyiyi*KYKKNKMRILYLASLLFLITLYLSPTFGWGGGRDCES
HRSEYTCKSDRSCAYLPFVSCCGKKEFFCVNRDHRNCCDDLSCAKNTRTGEIFEIWSSCK
PHRDFVPYHSPNTTTCESLGCEARGMECEWVESSPCYGTSCCPRIPRCVG---

---hqyremftke*rm*maslstrfqllq**krstlscq*cipttl*sysmsn*fll*nd
*r*snly*skttgttttllnlcraiism*srwndlysstkqlyqy*ipmlpissnlyssi
nyccfnhcnncincrnynlcnycrynnwwnynwwfnf***ccflsr**rcllitikqccf
kcrfk*tik*rrfk*csilcfk*rsilclk*csiiisfkfsciiscklrklrklishf

Frame C:
kffffiqfcnykkrnqiiiyiyknikkik*efyi*llfyf*lhyiyhqllvgvvaeivkv
ivqnihvnqievvhiyhlyhvvvrknfsvliviieivvmiyhvlktrelvkslrfgqvvn
hieilyhiihqiqphvnhwvvkqevwnvngxnhhhvmellvaqefqdvlx---

---TNIVKCSPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCKMI
RGNPTCIKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPS
TTAASTIATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSAAS
SAASSEPSSSAASSSAPSSASSSAPSSASSSAPSSSASSSAASSAASSESSESSSATS
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB244 (SLB244Q) /CSM/SL/SLB2-B/SLB244Q.Seq.d/                       1403   0.0
SLB171 (SLB171Q) /CSM/SL/SLB1-C/SLB171Q.Seq.d/                       1403   0.0
SLB132 (SLB132Q) /CSM/SL/SLB1-B/SLB132Q.Seq.d/                       1403   0.0
SLA236 (SLA236Q) /CSM/SL/SLA2-B/SLA236Q.Seq.d/                       1403   0.0
SFK752 (SFK752Q) /CSM/SF/SFK7-C/SFK752Q.Seq.d/                       1403   0.0
SFI829 (SFI829Q) /CSM/SF/SFI8-B/SFI829Q.Seq.d/                       1403   0.0
SFI626 (SFI626Q) /CSM/SF/SFI6-B/SFI626Q.Seq.d/                       1403   0.0
SFI550 (SFI550Q) /CSM/SF/SFI5-C/SFI550Q.Seq.d/                       1403   0.0
SFI545 (SFI545Q) /CSM/SF/SFI5-B/SFI545Q.Seq.d/                       1403   0.0
SFI229 (SFI229Q) /CSM/SF/SFI2-B/SFI229Q.Seq.d/                       1403   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.  1404   0.0    7
BJ170760|BJ170760.1 Physcomitrella patens subsp. patens cDNA clone:pph26m16, 3' end, single read.   190   3e-44  1
AF279135|AF279135.1 Dictyostelium discoideum spore coat structural protein SP65 (cotE) gene, complete cds.    64   5e-06  1
BX511123|BX511123.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    46   0.007  7
AC145563|AC145563.2 Mus musculus BAC clone RP24-75L3 from chromosome Y, complete sequence.    34   0.093  8
AY355260|AY355260.1 Human rhinovirus 79 VP1 capsid protein gene, partial cds.    48   0.100  2
AY450541|AY450541.1 Human rhinovirus 79 polyprotein mRNA, partial cds.    48   0.15   2
AC134329|AC134329.1 Mus musculus chromosome UNK clone RP24-310D17, WORKING DRAFT SEQUENCE, 12 unordered pieces.    42   0.31   5
AC079746|AC079746.2 Homo sapiens chromosome 7 clone CTD-2353O2, WORKING DRAFT SEQUENCE, 19 unordered pieces.    48   0.32   1
AC145891|AC145891.4 Pan troglodytes BAC clone RP43-20A24 from 7, complete sequence.    48   0.32   1
dna update 2004. 6.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       320   9e-94
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   316   1e-91
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   126   2e-31
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    96   2e-18
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    63   2e-08
B25439(B25439)Beejin protein - slime mold (Dictyostelium discoid...    62   4e-08
AC117076_50(AC117076|pid:none) Dictyostelium discoideum chromoso...    48   1e-07
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    57   1e-06
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    57   1e-06
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    53   2e-05
protein update 2009. 4.22
PSORT 

 psg: 0.96  gvh: 0.77  alm: 0.45  top: 0.10  tms: 0.00  mit: 0.32  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: nuclear

>> prediction for AFH810 is exc
5' end seq. ID AFH810F
5' end seq. 
>AFH810F.Seq
AAAAATTCTTTTTCTTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATAT
ATATATATAAAAATATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATT
TTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAG
TCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATC
ATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGA
TTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAA
ACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTGGG
TTGTGAAGCAAGAGGTATGGAATGTGAATGGGTNGAATCATCACCATGTTATGGAACTTC
TTGTTGCCCAAGAATTCCAAGATGTGTTGGNC----------
Length of 5' end seq. 512
3' end seq. ID AFH810Z
3' end seq. 
>AFH810Z.Seq
----------GCACCAATATCGTGAAATGTTCACCAAAGAATGACGAATGTAAATGGCAT
CGTTGTCCACCAGGTTCCAGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTTGCCAAT
AATGTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATG
ATTAGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCA
ACTTGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCA
AGCAATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCA
TCAACTACTGCTGCTTCAACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCT
GCAACTACTGCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGT
AGTGCTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCT
TCAAGTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCT
GCTTCAAGTAGCGCTCCATCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGT
TCAGCTGCATCATCAGCTGCAAGCTCAGAAAGCTCAGAAAGCTCATCAGCCACTTCAT
Length of 3' end seq. 708
Connected seq. ID AFH810P
Connected seq. 
>AFH810P.Seq
AAAAATTCTTTTTCTTCATTCAATTTTGTAATTATAAAAAAAGGAATCAAATAATTATAT
ATATATATAAAAATATAAAAAAAATAAAATGAGAATTTTATATTTAGCTTCTCTTTTATT
TTTAATTACATTATATCTATCACCAACTTTTGGTTGGGGTGGTGGCAGAGATTGTGAAAG
TCATCGTTCAGAATATACATGTAAATCAGATAGAAGTTGTGCATATTTACCATTTGTATC
ATGTTGTGGTAAGAAAGAATTTTTCTGTGTTAATCGTGATCATAGAAATTGTTGTGATGA
TTTATCATGTGCTAAAAACACGAGAACTGGTGAAATCTTTGAGATTTGGTCAAGTTGTAA
ACCACATAGAGATTTTGTACCATATCATTCACCAAATACAACCACATGTGAATCATTGGG
TTGTGAAGCAAGAGGTATGGAATGTGAATGGGTNGAATCATCACCATGTTATGGAACTTC
TTGTTGCCCAAGAATTCCAAGATGTGTTGGNC----------GCACCAATATCGTGAAAT
GTTCACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCA
ATAGTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTTTGTAAAGTTA
CTCAATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACTTGTATTAAAG
CAAGACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCATCAGCATGTA
ATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGATTCCCAT
GTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCATTGCAA
CAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTG
GAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCG
CTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAA
GTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCT
CAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAGCTCAG
AAAGCTCAGAAAGCTCATCAGCCACTTCAT
Length of connected seq. 1220
Full length Seq ID -
Full length Seq. -
Length of full length seq. -