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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFA886
Library SF
(Link to library)
Clone ID SFA886
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SFA886P
(Link to Original site)
Representative DNA sequence 
>SFA886 (SFA886Q) /CSM/SF/SFA8-D/SFA886Q.Seq.d/
AAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCA
ACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCA
GTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAA
TCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAA
CCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGAT
CTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTA
GCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAA
ATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCA
CATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCAXXXXXXXXXXAATTA
GGTGATCTNTACANANAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTT
GATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTT
GGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCA
TGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCC
GCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACT
GAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGT
GCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAG
TTAGGTGAAAACCATAACTATG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDK
SRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSL
AVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMILP---

---LGDLYXXFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGI
EKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQ
IKTGAPCRSERLAKYNQLVRINEELGENHNY
Translated Amino Acid sequence (All Frames) 
Frame A:
KMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDK
SRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSL
AVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKLAMQEFMILP---

---n*vixtxnslkniqsfqlkihlikmignhtln*qhqlifkllvmiyllptqkelrlv
lkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswlaivqvklkipslli*llv*vlv
klklvlhvdqkd*pntinlleltks*vktitm

Frame B:
k*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhqlvfmklln*emvin
qdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqikvn*vptlslqsv*
lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin*lcknl*fy---

---ir*sxxxih*risnhfn*rsi*sr*lgiih*insis*ysncw**ftcyqprkn*dwy
*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*psfr*n*rylhc*fscwfryws
n*nwcsm*irkisqiqstc*n*rrvr*kp*l

Frame C:
nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssircinwyl*sc*ikrw**i
kilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk*r*ircqrypcsqfs
clqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**isyariydft---

---LGDLYXXFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGI
EKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQ
IKTGAPCRSERLAKYNQLVRINEELGENHNY
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA886 (SFA886Q) /CSM/SF/SFA8-D/SFA886Q.Seq.d/                       1869   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1041   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                       1041   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                       1041   0.0
SLA770 (SLA770Q) /CSM/SL/SLA7-C/SLA770Q.Seq.d/                       1041   0.0
SHG555 (SHG555Q) /CSM/SH/SHG5-C/SHG555Q.Seq.d/                       1041   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1041   0.0
SFH308 (SFH308Q) /CSM/SF/SFH3-A/SFH308Q.Seq.d/                       1041   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1041   0.0
SFC211 (SFC211Q) /CSM/SF/SFC2-A/SFC211Q.Seq.d/                       1041   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           86   4e-60  9
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-56  9
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-56  9
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   2e-56  9
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   2e-54  8
X80474|X80474.1 N.frontalis enolase gene.                          64   1e-51  8
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   2e-45  4
AX489475|AX489475.1 Sequence 6775 from Patent WO02053728.          56   1e-40  9
M93712|M93712.1 Candida albicans (clone lambda-C1.d) enolase mRNA, complete cds.    56   1e-40  9
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.          50   6e-39  9
dna update 2003.12.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Z68318_5(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   377   e-103
BC009018_1(BC009018|pid:none) Mus musculus enolase 2, gamma neur...   334   3e-90
(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   334   3e-90
EF676265_1(EF676265|pid:none) Picea sitchensis clone WS02725_D05...   333   7e-90
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   239   1e-61
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   238   2e-61
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   238   2e-61
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   237   5e-61
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   235   1e-60
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   235   1e-60
protein update 2009. 5.22
PSORT 

 psg: 0.47  gvh: 0.46  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for SFA886 is cyt
5' end seq. ID SFA886F
5' end seq. 
>SFA886F.Seq
AAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCA
ACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCA
GTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAA
TCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAA
CCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGAT
CTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTA
GCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAA
ATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCA
CATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCA----------
Length of 5' end seq. 525
3' end seq. ID SFA886Z
3' end seq. 
>SFA886Z.Seq
----------AATTAGGTGATCTNTACANANAATTCATTAAAGAATATCCAATCATTTCA
ATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTT
GATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGT
ATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACT
GAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCAT
CGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGT
CAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTT
AGAATTAACGAAGAGTTAGGTGAAAACCATAACTATG
Length of 3' end seq. 447
Connected seq. ID SFA886P
Connected seq. 
>SFA886P.Seq
AAAATGAGCGTCATCAAATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCA
ACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCA
GTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAA
TCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAA
CCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGAT
CTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTA
GCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAA
ATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCA
CATGCAGGTAATAAATTAGCTATGCAAGAATTTATGATTTTACCA----------AATTA
GGTGATCTNTACANANAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTT
GATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTT
GGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCA
TGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCC
GCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACT
GAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGT
GCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAG
TTAGGTGAAAACCATAACTATG
Length of connected seq. 972
Full length Seq ID -
Full length Seq. -
Length of full length seq. -