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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFA408
Library SF
(Link to library)
Clone ID SFA408
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10972-1
Original site URL
Representative seq. ID SFA408P
(Link to Original site)
Representative DNA sequence 
>SFA408 (SFA408Q) /CSM/SF/SFA4-A/SFA408Q.Seq.d/
ACTGTTGGCCTACTGGNATTCATCGATAATAGNTTTTTTAAAAAAATTTGATAAAAACAA
AACAAAAGAATTTTCACAAAATATAATGCAACCACAACAACCAATTGGTGCACCTCATTT
AGGTGGTGGTGAATTATTCAACAACAATATCGATTTGGAAAATAACCAAACACACGAATA
TAGAGGAATTATCCAAAGATTAAAATCAATCATTGTAAATTCAAAAGATGATAATTTAAT
GAGTATGATGAGAATGGTATTCTCTGAATCTAATCTTGGTAAATCATTTTTAGTTGATGA
TTACAATCAAAAAAGAATACCAGATATTGGAGGACCAATTCAAAATTTTGTTTTTTTAGG
AGATGTTGAACCATCACAAATGTCACCTACACCATTAAATTCATTTTTTTCAACACATGC
AGCAAATTCTTCAATTTCAGGATCACTTCCACCATTAAGATCTCCAAGAAAGGTTGAATC
TGTTCCATCAATTCCAGTTTTACCATTATXXXXXXXXXXTCTATGGTATTCATGGTATTC
AAAAGCTACTTCAATACCTCTCANCCGATCAAGTNGAATCCATCATTGCCTCCATCAAGG
GTAAGGTAATACAATTATCAAAGGATAACAAGGGTAACTACTTGATTCAATCATTTTTGA
AACAATTTTCACCAGAGGTCAATCAATTTGTTTGTGATGCAATCATGGGTAATATTGAGG
AGATTTGTACCCATAAAGTTGGCTGTACAGTCGTCAATCGTTGTATCGATAATGCCAATC
CTGAGCAACTAGAGAAATTGGTCGATAAAATCACTGAACATTCCCTCAAATTGGTACAAG
ATCAATTTGGCAACTATGTCGTTCAACATCTCCTCTCAAAGAATAAAGCTTACTCTTCAA
AATTGATTGTCTCACTCAAAGATAACATTGCTGAATTATCCATCCAAAAGTTTAGCTCCA
ATGTNAANAAAAAGTGTTTACAATTGGCTGATACTCCAACCTATGAATTGATCATCAAGG
CTTTGACAGAAGCTGATATCCTCAGTTTGCTTCAAGATAAATATGCCAACTTCGTGATCC
AAACCGCTTTGGACGTCAGTAATGATACTCAACACGCAAAACTTGTAAAAATTATTGTAC
CCTACATTCATCAAATTAAGACTNCCANACGTAATTCATATTCAAAAGTAAGATACTNCA
AGTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LLAYWXSSIIXFLKKFDKNKTKEFSQNIMQPQQPIGAPHLGGGELFNNNIDLENNQTHEY
RGIIQRLKSIIVNSKDDNLMSMMRMVFSESNLGKSFLVDDYNQKRIPDIGGPIQNFVFLG
DVEPSQMSPTPLNSFFSTHAANSSISGSLPPLRSPRKVESVPSIPVLPL---

---YGIHGIQKLLQYLSXDQVESIIASIKGKVIQLSKDNKGNYLIQSFLKQFSPEVNQFV
CDAIMGNIEEICTHKVGCTVVNRCIDNANPEQLEKLVDKITEHSLKLVQDQFGNYVVQHL
LSKNKAYSSKLIVSLKDNIAELSIQKFSSNVXKKCLQLADTPTYELIIKALTEADILSLL
QDKYANFVIQTALDVSNDTQHAKLVKIIVPYIHQIKTXXRNSYSKVRYXKF
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxfidnxffkki**kqnkriftkynattttnwctsfrww*iiqqqyrfgk*pntri
*rnypkikinhckfkr**fneydengil*i*sw*iifs**lqskkntrywrtnskfcffr
rc*titnvtytikfiffntcskffnfritstikiskkg*icsinssfti---

---smvfmvfksyfntsxpikxnpslppsrvr*ynyqritrvtt*fnhf*nnfhqrsinl
fvmqswvilrrfvpiklavqssivvsimpilsn*rnwsikslnipsnwykinlatmsfni
ssqrikltlqn*lshskitllnypskslapmxxksvynwlilqpmn*ssrl*qklissvc
fkinmpts*skplwtsvmilntqnl*kllyptfiklrlpxvihiqk*dtxs

Frame B:
LLAYWXSSIIXFLKKFDKNKTKEFSQNIMQPQQPIGAPHLGGGELFNNNIDLENNQTHEY
RGIIQRLKSIIVNSKDDNLMSMMRMVFSESNLGKSFLVDDYNQKRIPDIGGPIQNFVFLG
DVEPSQMSPTPLNSFFSTHAANSSISGSLPPLRSPRKVESVPSIPVLPL---

---lwyswyskatsiplxrssxihhclhqg*gntiikg*qg*lldsiifetiftrgqsic
l*cnhg*y*gdlyp*swlysrqslyr*cqs*atreigr*nh*tfpqigtrsiwqlcrsts
plke*sllfkidcltqr*hc*iihpkv*lqcxxkvftig*ysnl*idhqgfdrs*ypqfa
sr*icqlrdpnrfgrq**ystrktcknyctlhssn*dxxt*fifkskilqv

Frame C:
cwptgihr**xf*knliktkqknfhki*cnhnnqlvhli*vvvnysttisiwkitkhtni
eelskd*nqsl*iqkmii**v**ewyslnlilvnhf*lmitikkeyqiledqfkilff*e
mlnhhkchlhh*ihffqhmqqilqfqdhfhh*dlqerlnlfhqfqfyhy---

---YGIHGIQKLLQYLSXDQVESIIASIKGKVIQLSKDNKGNYLIQSFLKQFSPEVNQFV
CDAIMGNIEEICTHKVGCTVVNRCIDNANPEQLEKLVDKITEHSLKLVQDQFGNYVVQHL
LSKNKAYSSKLIVSLKDNIAELSIQKFSSNVXKKCLQLADTPTYELIIKALTEADILSLL
QDKYANFVIQTALDVSNDTQHAKLVKIIVPYIHQIKTXXRNSYSKVRYXKF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA408 (SFA408Q) /CSM/SF/SFA4-A/SFA408Q.Seq.d/                       2044   0.0
SHA672 (SHA672Q) /CSM/SH/SHA6-C/SHA672Q.Seq.d/                       1247   0.0
AFJ353 (AFJ353Q) /CSM/AF/AFJ3-C/AFJ353Q.Seq.d/                        993   0.0
CHR212 (CHR212Q) /CSM/CH/CHR2-A/CHR212Q.Seq.d/                        950   0.0
CHE817 (CHE817Q) /CSM/CH/CHE8-A/CHE817Q.Seq.d/                        759   0.0
CFB402 (CFB402Q) /CSM/CF/CFB4-A/CFB402Q.Seq.d/                        696   0.0
CHH864 (CHH864Q) /CSM/CH/CHH8-C/CHH864Q.Seq.d/                        601   e-170
CHR786 (CHR786Q) /CSM/CH/CHR7-D/CHR786Q.Seq.d/                        599   e-170
CHE680 (CHE680Q) /CSM/CH/CHE6-D/CHE680Q.Seq.d/                        591   e-167
CHK426 (CHK426Q) /CSM/CH/CHK4-B/CHK426Q.Seq.d/                        480   e-134
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X63867|X63867.1 S.cerevisiae SAC6 gene encoding fimbrin.           38   1.1    2
CD398919|CD398919.1 Gm_ck20311 Soybean induced by Salicylic Acid Glycine max cDNA 3', mRNA sequence.    46   1.1    1
CD397244|CD397244.1 Gm_ck17944 Soybean induced by Salicylic Acid Glycine max cDNA 3', mRNA sequence.    46   1.1    1
BU765150|BU765150.1 sas23e05.y1 Gm-c1080 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1080-4809 5', mRNA sequence.    46   1.1    1
AL445564|AL445564.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 2/3.    36   1.1    12
AC144405|AC144405.23 Medicago truncatula clone mth2-16m18, WORKING DRAFT SEQUENCE, 10 unordered pieces.    36   1.9    8
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    32   2.4    10
AC142143|AC142143.15 Medicago truncatula clone mth2-25c22, WORKING DRAFT SEQUENCE, 18 unordered pieces.    34   3.3    7
AC133358|AC133358.2 Rattus norvegicus clone CH230-40K10, WORKING DRAFT SEQUENCE, 5 unordered pieces.    44   4.4    1
Z83239|Z83239.1 Caenorhabditis elegans cosmid T09F5.               44   4.4    1
dna update 2003.12.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q92359) RecName: Full=Pumilio domain-containing protein C6G9.14...   165   3e-39
AE017343_431(AE017343|pid:none) Cryptococcus neoformans var. neo...   160   6e-38
AP007151_730(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   158   4e-37
CU638743_632(CU638743|pid:none) Podospora anserina genomic DNA c...   154   8e-36
CR954215_201(CR954215|pid:none) Ostreococcus tauri strain OTTH05...   153   1e-35
EF068153_1(EF068153|pid:none) Hortaea werneckii pumilio-family R...   150   6e-35
S57889_1(S57889|pid:none) YGL023=putative regulatory protein [Sa...   149   2e-34
CR380947_15(CR380947|pid:none) Candida glabrata strain CBS138 ch...   147   9e-34
CR382121_377(CR382121|pid:none) Kluyveromyces lactis strain NRRL...   143   1e-32
BT009970_1(BT009970|pid:none) Drosophila melanogaster RE63138 fu...   142   2e-32
protein update 2009. 5.21
PSORT 

 psg: 0.90  gvh: 0.36  alm: 0.43  top: 0.67  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for SFA408 is nuc
5' end seq. ID SFA408F
5' end seq. 
>SFA408F.Seq
ACTGTTGGCCTACTGGNATTCATCGATAATAGNTTTTTTAAAAAAATTTGATAAAAACAA
AACAAAAGAATTTTCACAAAATATAATGCAACCACAACAACCAATTGGTGCACCTCATTT
AGGTGGTGGTGAATTATTCAACAACAATATCGATTTGGAAAATAACCAAACACACGAATA
TAGAGGAATTATCCAAAGATTAAAATCAATCATTGTAAATTCAAAAGATGATAATTTAAT
GAGTATGATGAGAATGGTATTCTCTGAATCTAATCTTGGTAAATCATTTTTAGTTGATGA
TTACAATCAAAAAAGAATACCAGATATTGGAGGACCAATTCAAAATTTTGTTTTTTTAGG
AGATGTTGAACCATCACAAATGTCACCTACACCATTAAATTCATTTTTTTCAACACATGC
AGCAAATTCTTCAATTTCAGGATCACTTCCACCATTAAGATCTCCAAGAAAGGTTGAATC
TGTTCCATCAATTCCAGTTTTACCATTAT----------
Length of 5' end seq. 509
3' end seq. ID SFA408Z
3' end seq. 
>SFA408Z.Seq
----------TCTATGGTATTCATGGTATTCAAAAGCTACTTCAATACCTCTCANCCGAT
CAAGTNGAATCCATCATTGCCTCCATCAAGGGTAAGGTAATACAATTATCAAAGGATAAC
AAGGGTAACTACTTGATTCAATCATTTTTGAAACAATTTTCACCAGAGGTCAATCAATTT
GTTTGTGATGCAATCATGGGTAATATTGAGGAGATTTGTACCCATAAAGTTGGCTGTACA
GTCGTCAATCGTTGTATCGATAATGCCAATCCTGAGCAACTAGAGAAATTGGTCGATAAA
ATCACTGAACATTCCCTCAAATTGGTACAAGATCAATTTGGCAACTATGTCGTTCAACAT
CTCCTCTCAAAGAATAAAGCTTACTCTTCAAAATTGATTGTCTCACTCAAAGATAACATT
GCTGAATTATCCATCCAAAAGTTTAGCTCCAATGTNAANAAAAAGTGTTTACAATTGGCT
GATACTCCAACCTATGAATTGATCATCAAGGCTTTGACAGAAGCTGATATCCTCAGTTTG
CTTCAAGATAAATATGCCAACTTCGTGATCCAAACCGCTTTGGACGTCAGTAATGATACT
CAACACGCAAAACTTGTAAAAATTATTGTACCCTACATTCATCAAATTAAGACTNCCANA
CGTAATTCATATTCAAAAGTAAGATACTNCAAGTTT
Length of 3' end seq. 686
Connected seq. ID SFA408P
Connected seq. 
>SFA408P.Seq
ACTGTTGGCCTACTGGNATTCATCGATAATAGNTTTTTTAAAAAAATTTGATAAAAACAA
AACAAAAGAATTTTCACAAAATATAATGCAACCACAACAACCAATTGGTGCACCTCATTT
AGGTGGTGGTGAATTATTCAACAACAATATCGATTTGGAAAATAACCAAACACACGAATA
TAGAGGAATTATCCAAAGATTAAAATCAATCATTGTAAATTCAAAAGATGATAATTTAAT
GAGTATGATGAGAATGGTATTCTCTGAATCTAATCTTGGTAAATCATTTTTAGTTGATGA
TTACAATCAAAAAAGAATACCAGATATTGGAGGACCAATTCAAAATTTTGTTTTTTTAGG
AGATGTTGAACCATCACAAATGTCACCTACACCATTAAATTCATTTTTTTCAACACATGC
AGCAAATTCTTCAATTTCAGGATCACTTCCACCATTAAGATCTCCAAGAAAGGTTGAATC
TGTTCCATCAATTCCAGTTTTACCATTAT----------TCTATGGTATTCATGGTATTC
AAAAGCTACTTCAATACCTCTCANCCGATCAAGTNGAATCCATCATTGCCTCCATCAAGG
GTAAGGTAATACAATTATCAAAGGATAACAAGGGTAACTACTTGATTCAATCATTTTTGA
AACAATTTTCACCAGAGGTCAATCAATTTGTTTGTGATGCAATCATGGGTAATATTGAGG
AGATTTGTACCCATAAAGTTGGCTGTACAGTCGTCAATCGTTGTATCGATAATGCCAATC
CTGAGCAACTAGAGAAATTGGTCGATAAAATCACTGAACATTCCCTCAAATTGGTACAAG
ATCAATTTGGCAACTATGTCGTTCAACATCTCCTCTCAAAGAATAAAGCTTACTCTTCAA
AATTGATTGTCTCACTCAAAGATAACATTGCTGAATTATCCATCCAAAAGTTTAGCTCCA
ATGTNAANAAAAAGTGTTTACAATTGGCTGATACTCCAACCTATGAATTGATCATCAAGG
CTTTGACAGAAGCTGATATCCTCAGTTTGCTTCAAGATAAATATGCCAACTTCGTGATCC
AAACCGCTTTGGACGTCAGTAATGATACTCAACACGCAAAACTTGTAAAAATTATTGTAC
CCTACATTCATCAAATTAAGACTNCCANACGTAATTCATATTCAAAAGTAAGATACTNCA
AGTTT
Length of connected seq. 1195
Full length Seq ID -
Full length Seq. -
Length of full length seq. -