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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFA401
Library SF
(Link to library)
Clone ID SFA401
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13873-1
Original site URL
Representative seq. ID SFA401P
(Link to Original site)
Representative DNA sequence 
>SFA401 (SFA401Q) /CSM/SF/SFA4-A/SFA401Q.Seq.d/
GNATAGTAGNAGGTAATCCAACATTAGNAAGTTGAAGTATGGACAAGTGAAGGNAATGTT
TTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGA
TGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAA
TTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAA
AATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGG
TGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTA
CATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTAT
TAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGNAXXXXX
XXXXXATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAATTGGTATTGATTCTGC
TGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAATATAGG
ATCCTCACATTTGAAGTCTAGTTTAGAATAGTGGTGAGCAATTAATGTCTAGATATTTAG
AGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGG
AAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGATGATT
TACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCAT
TATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTG
CAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATT
TCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATNCTGGTGCACCTTGT
AGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTTGAAAAA
CTTCATAGATCAAATGGTAATTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
xsxr*SNIXKLKYGQVKXMFSAKVPSGASTGIHEAFELRDGDKSRYFGYGVLNAIVNVNN
LISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVGVSMAVARAAASIKGVPLYRY
ISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLX---

---AINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLE*w*ainv*if
rdfkkvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckf
iiienksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc*scswswyw*nkxlvhl
vdlkdyqniid**klkknlknfidqmvi
Translated Amino Acid sequence (All Frames) 
Frame A:
x**xviqh*xvevwtsegnvfsqstkwsinwys*si*ikrw**kslfwiwcfecnc*ck*
fnitnieryes**sksnr**nd*igwyke*iktwvqcnrwcingsgkssrfy*rcalvpl
hftigwk**ysftstmf*cy*wwktcr**ttisrvyax---

---mlsiiraikmllklvlilllqnfgiqrlqnmiwisri*dphi*slv*nsgeqlmsry
leilkkypiaffedpfgeddwenhgkitaaignkiqiigddllctnperikkaisektvn
slllkinqigtltetieaaklakeagwgclvshrsgetddhfiadlvvglgtgeikxwct
l*i*kiiki**inen*rrt*kts*ikw*f

Frame B:
xsxr*SNIXKLKYGQVKXMFSAKVPSGASTGIHEAFELRDGDKSRYFGYGVLNAIVNVNN
LISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVGVSMAVARAAASIKGVPLYRY
ISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLX---

---cyqsfglsrcy*nwy*fccfrileskdfki*ygfqeyriltfev*frivvsn*cldi
*rf*ksiqlhslkihlvkmigkimvklqlqlvikfkslvmiyfvqiqkelkkqflrkl*i
hyy*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisllil*lvlvlvk*nxgap
crferlskynrlmkieeeleklhrsngns

Frame C:
ivxgnptlxs*smdk*rxcfqpkyqvehqlvfmkhln*emvikvvildmvf*mqllm*ii
*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpmqslvyqwqwqeqplllkvcpctvt
fhnwleivifvyqyhvlmllmvenmqvidyhfkslc*---

---AINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLE*w*ainv*if
rdfkkvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckf
iiienksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc*scswswyw*nkxlvhl
vdlkdyqniid**klkknlknfidqmvi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA401 (SFA401Q) /CSM/SF/SFA4-A/SFA401Q.Seq.d/                       2095   0.0
SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/                       1195   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1195   0.0
CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       1193   0.0
CHD848 (CHD848Q) /CSM/CH/CHD8-B/CHD848Q.Seq.d/                       1187   0.0
CHB459 (CHB459Q) /CSM/CH/CHB4-C/CHB459Q.Seq.d/                       1185   0.0
CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       1179   0.0
CFK240 (CFK240Q) /CSM/CF/CFK2-B/CFK240Q.Seq.d/                       1174   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1174   0.0
CHC156 (CHC156Q) /CSM/CH/CHC1-C/CHC156Q.Seq.d/                       1172   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   5e-27  5
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   2e-16  5
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   2e-16  5
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   2e-16  5
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   2e-16  5
L23324|L23324.1 Schistosoma japonicum enolase mRNA, complete cds.    68   4e-15  5
AF063247|AF063247.1 Pneumocystis carinii f. sp. ratti enolase mRNA, complete cds.    64   5e-14  5
BU723146|ENO_SCHJA ENOLASE (2-PHOSPHOGLYCERATE DEHYDRATASE) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE), mRNA sequence.    68   7e-13  4
BU725524|ENO_SCHJA ENOLASE (2-PHOSPHOGLYCERATE DEHYDRATASE) (2-PHOSPHO-D-GLYCERATE HYDRO-LYASE), mRNA sequence.    68   8e-13  4
AZ690037|AZ690037.1 ENTKP77TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   8e-13  3
dna update 2003.12.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   177   1e-88
AF363629_1(AF363629|pid:none) Trichinella spiralis enolase (Eno)...   166   2e-85
Z68318_3(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   162   2e-84
(Q27527) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   162   2e-84
AY725787_1(AY725787|pid:none) Oncometopia nigricans enolase mRNA...   175   6e-84
(Q9PVK2) RecName: Full=Alpha-enolase;          EC=4.2.1.11; AltN...   164   3e-83
(P51555) RecName: Full=Enolase 1;          EC=4.2.1.11; AltName:...   169   1e-82
(Q7RA60) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   166   1e-82
AF348920_1(AF348920|pid:none) Mastocarpus papillatus enolase 1 (...   169   5e-82
(Q27877) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   156   6e-82
protein update 2009. 5.21
PSORT 

 psg: 0.72  gvh: 0.48  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 44.0 %: nuclear

>> prediction for SFA401 is cyt
5' end seq. ID SFA401F
5' end seq. 
>SFA401F.Seq
GNATAGTAGNAGGTAATCCAACATTAGNAAGTTGAAGTATGGACAAGTGAAGGNAATGTT
TTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGA
TGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAA
TTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAA
AATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGG
TGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTA
CATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTAT
TAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGNA-----
-----
Length of 5' end seq. 475
3' end seq. ID SFA401Z
3' end seq. 
>SFA401Z.Seq
----------ATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAATTGGTATTGAT
TCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAAT
ATAGGATCCTCACATTTGAAGTCTAGTTTAGAATAGTGGTGAGCAATTAATGTCTAGATA
TTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGA
TTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGA
TGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAA
TTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAA
ACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGA
TCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATNCTGGTGCAC
CTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTTG
AAAAACTTCATAGATCAAATGGTAATTCA
Length of 3' end seq. 619
Connected seq. ID SFA401P
Connected seq. 
>SFA401P.Seq
GNATAGTAGNAGGTAATCCAACATTAGNAAGTTGAAGTATGGACAAGTGAAGGNAATGTT
TTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGA
TGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAA
TTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAA
AATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGG
TGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTA
CATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTAT
TAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGNA-----
-----ATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAATTGGTATTGATTCTGC
TGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAATATAGG
ATCCTCACATTTGAAGTCTAGTTTAGAATAGTGGTGAGCAATTAATGTCTAGATATTTAG
AGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGG
AAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGATGATT
TACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCAT
TATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTG
CAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATT
TCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATNCTGGTGCACCTTGT
AGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTTGAAAAA
CTTCATAGATCAAATGGTAATTCA
Length of connected seq. 1094
Full length Seq ID -
Full length Seq. -
Length of full length seq. -