>SFA328 (SFA328Q) /CSM/SF/SFA3-B/SFA328Q.Seq.d/
XXXXXXXXXXAATTTCACAAATCTCATGCAAAGANATCNCTTCATATGTTCCAAGAGGTA
ACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCATTTAACTGAA
TTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACCATTCTCAAGGGTT
GAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGAAAGAAAATTA
TCACTTATGATCCTTGATAAGAAATGTAATGGTATCCTCGATCAAGGTACTGGTACTTTA
ATTGTATTTGAAGAACAAAAANAAGATAAATTATTTAATTG

---FHKSHAKXSLHMFQEVTKEFSDQLSDDPIIHSHLTELYSNLLEQNLCRIIEPFSRVE
ISHIAKLIDLPVDVVERKLSLMILDKKCNGILDQGTGTLIVFEEQKXDKLFN

Frame A:
---isqisckxixsyvprgnqrif*sii**snhs*sfn*ii**ss*tkfmsyy*tilkg*
nftyckin*fts*cc*kkiitydp**em*wyprsrywyfnci*rtkxr*ii*l


Frame B:
---FHKSHAKXSLHMFQEVTKEFSDQLSDDPIIHSHLTELYSNLLEQNLCRIIEPFSRVE
ISHIAKLIDLPVDVVERKLSLMILDKKCNGILDQGTGTLIVFEEQKXDKLFN


Frame C:
---ftnlmqrxxficskr*pknflinylmiqsfivi*lnyiviflnkiyvvllnhsqglk
fhilqn*liyqlmllkenyhl*slirnvmvssikvlvl*lylknkxkinyli

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA328 (SFA328Q) /CSM/SF/SFA3-B/SFA328Q.Seq.d/                        638   0.0
VSD281 (VSD281Q) /CSM/VS/VSD2-D/VSD281Q.Seq.d/                        615   e-175
VFO795 (VFO795Q) /CSM/VF/VFO7-D/VFO795Q.Seq.d/                        615   e-175
VFN509 (VFN509Q) /CSM/VF/VFN5-A/VFN509Q.Seq.d/                        615   e-175
VFM634 (VFM634Q) /CSM/VF/VFM6-B/VFM634Q.Seq.d/                        615   e-175
VFK702 (VFK702Q) /CSM/VF/VFK7-A/VFK702Q.Seq.d/                        615   e-175
VFK121 (VFK121Q) /CSM/VF/VFK1-A/VFK121Q.Seq.d/                        615   e-175
VFI186 (VFI186Q) /CSM/VF/VFI1-D/VFI186Q.Seq.d/                        615   e-175
VFH786 (VFH786Q) /CSM/VF/VFH7-D/VFH786Q.Seq.d/                        615   e-175
VFH659 (VFH659Q) /CSM/VF/VFH6-C/VFH659Q.Seq.d/                        615   e-175

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BE320243|BE320243.2 NF024E04RT1F1023 Developing root Medicago truncatula cDNA clone NF024E04RT 5', mRNA sequence.    68   4e-09  2
AW126253|AW126253.2 N100360e rootphos(-) Medicago truncatula cDNA clone MHRP-5A2, mRNA sequence.    68   7e-09  2
AJ497077|AJ497077.1 Medicago truncatula EST, clone mt--abc955104c04.    68   8e-09  2
CB893586|CB893586.1 EST646378 HOGA Medicago truncatula cDNA clone HOGA-28K10, mRNA sequence.    68   1e-08  2
BG648576|BG648576.1 EST510195 HOGA Medicago truncatula cDNA clone pHOGA-23M13 5' end, mRNA sequence.    68   1e-08  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    32   0.005  12
AC122060|AC122060.2 Mus musculus chromosome UNK clone RP24-468P4, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48   0.008  7
AC116963|AC116963.1 Dictyostelium discoideum chromosome 2 map 4790445-4846595 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    34   0.010  5
BQ412650|BQ412650.1 GA__Ed0060G10r Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ed0060G10r, mRNA sequence.    44   0.076  2
AC125272|AC125272.2 Mus musculus clone RP24-155E2, LOW-PASS SEQUENCE SAMPLING.    48   0.076  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UB5) RecName: Full=26S proteasome non-ATPase regulatory subu...   205   4e-52
AM424416_1(AM424416|pid:none) Vitis vinifera contig VV78X196128....   138   7e-32
EF146969_1(EF146969|pid:none) Populus trichocarpa clone WS01225_...   137   9e-32
AY230837_1(AY230837|pid:none) Arabidopsis thaliana 26S proteasom...   137   1e-31
AF083890_1(AF083890|pid:none) Arabidopsis thaliana 19S proteosom...   137   1e-31
AY230834_1(AY230834|pid:none) Arabidopsis thaliana 26S proteasom...   137   1e-31
A86414(A86414) hypothetical protein F28N24.15 - Arabidopsis thal...   137   1e-31
BT053774_1(BT053774|pid:none) Zea mays full-length cDNA clone ZM...   133   2e-30
EU969379_1(EU969379|pid:none) Zea mays clone 329471 26S proteaso...   132   4e-30
AK064788_1(AK064788|pid:none) Oryza sativa Japonica Group cDNA c...   132   4e-30

 psg: 0.70  gvh: 0.28  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for SFA328 is cyt

>SFA328Z.Seq
----------AATTTCACAAATCTCATGCAAAGANATCNCTTCATATGTTCCAAGAGGTA
ACCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCATTTAACTGAA
TTATATAGTAATCTTCTTGAACAAAATTTATGTCGTATTATTGAACCATTCTCAAGGGTT
GAAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGAAAGAAAATTA
TCACTTATGATCCTTGATAAGAAATGTAATGGTATCCTCGATCAAGGTACTGGTACTTTA
ATTGTATTTGAAGAACAAAAANAAGATAAATTATTTAATTG