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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFA308
Library SF
(Link to library)
Clone ID SFA308
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID SFA308P
(Link to Original site)
Representative DNA sequence 
>SFA308 (SFA308Q) /CSM/SF/SFA3-A/SFA308Q.Seq.d/
ACTGTTGGCCTACTGGCATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTT
TTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGGAAATTTTAGATTCACGTGGTAA
CCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGC
TGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGA
TAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCAT
TCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGAT
CGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAG
TTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTC
AGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGX
XXXXXXXXXTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAAT
GGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAA
AAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGAT
CCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAA
ATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAG
AAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
llaywhyfiffylylyiyhflk*ASSNQLKPEEILDSRGNPTVEVDLYTEKDGVVSSFRA
AVPSGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMI
DLDGTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVING-
--

---LVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIED
PFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVT
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllallyfflslsiyisffkmsviksikargnfrftw*pnc*s*plhrkrwccfiiqs
cssircinwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*ind
rsrwypk*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqw-
--

---LVKIGMDCAASEFKVENGYDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIED
PFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVT

Frame B:
llaywhyfiffylylyiyhflk*ASSNQLKPEEILDSRGNPTVEVDLYTEKDGVVSSFRA
AVPSGASTGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMI
DLDGTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVING-
--

---*lklvwivlplnsklkmvtiltsklktmtvqllslvkn*vistenslkniqsfqlki
hlikmignhtln*qhqlifkllvmiyllptqkelrlvlkrkhvmlfy*kltklvqs

Frame C:
cwptgitlfffisiyiyiif*nerhqin*sqrkf*ihvvtqllkltftpkkmvlfhhsel
qfhqvhqlvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**s
ismvpqikvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmv-
--

---s*nwyglccl*iqs*kwlrs*lqn*kq*rfscylw*kir*slqrih*risnhfn*rs
i*sr*lgiih*insis*ysncw**ftcyqprkn*dwy*kesm*csfiks*pnwfsh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA308 (SFA308Q) /CSM/SF/SFA3-A/SFA308Q.Seq.d/                       1653   0.0
VFH161 (VFH161Q) /CSM/VF/VFH1-C/VFH161Q.Seq.d/                        983   0.0
VFF123 (VFF123Q) /CSM/VF/VFF1-A/VFF123Q.Seq.d/                        983   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                        983   0.0
VFB851 (VFB851Q) /CSM/VF/VFB8-C/VFB851Q.Seq.d/                        983   0.0
SSM734 (SSM734Q) /CSM/SS/SSM7-B/SSM734Q.Seq.d/                        983   0.0
SHG555 (SHG555Q) /CSM/SH/SHG5-C/SHG555Q.Seq.d/                        983   0.0
SFK326 (SFK326Q) /CSM/SF/SFK3-B/SFK326Q.Seq.d/                        983   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                        983   0.0
SFH308 (SFH308Q) /CSM/SF/SFH3-A/SFH308Q.Seq.d/                        983   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-42  7
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76   2e-42  7
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76   2e-42  7
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76   9e-41  6
X80474|X80474.1 N.frontalis enolase gene.                          64   2e-35  7
BM165969|BM165969.1 EST568492 PyBS Plasmodium yoelii yoelii cDNA clone PYCMZ58 5' end, mRNA sequence.    92   4e-32  3
BM163099|BM163099.1 EST565622 PyBS Plasmodium yoelii yoelii cDNA clone PYCLA85 5' end, mRNA sequence.    92   4e-32  3
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92   4e-32  3
BM164428|BM164428.1 EST566951 PyBS Plasmodium yoelii yoelii cDNA clone PYCLU01 5' end, mRNA sequence.    92   4e-32  3
BM160389|BM160389.1 EST562912 PyBS Plasmodium yoelii yoelii cDNA clone PYCJQ69 5' end, mRNA sequence.    92   4e-32  3
dna update 2003.12.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Z68318_5(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   167   8e-79
(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   288   2e-76
BC009018_1(BC009018|pid:none) Mus musculus enolase 2, gamma neur...   149   5e-66
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   200   6e-50
U85835_1(U85835|pid:none) Unidentified pseudophyllidean enolase ...   198   2e-49
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   198   2e-49
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   198   2e-49
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   197   4e-49
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   197   4e-49
CR859195_1(CR859195|pid:none) Pongo abelii mRNA; cDNA DKFZp459B1...   197   4e-49
protein update 2009. 5.21
PSORT 

 psg: 0.75  gvh: 0.46  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: Golgi

>> prediction for SFA308 is cyt
5' end seq. ID SFA308F
5' end seq. 
>SFA308F.Seq
ACTGTTGGCCTACTGGCATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTT
TTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGGAAATTTTAGATTCACGTGGTAA
CCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGC
TGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGA
TAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCAT
TCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGAT
CGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAG
TTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTC
AGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTG-
---------
Length of 5' end seq. 539
3' end seq. ID SFA308Z
3' end seq. 
>SFA308Z.Seq
----------TTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAA
TGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGA
AAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGA
TCCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCA
AATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAA
GAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACT
Length of 3' end seq. 342
Connected seq. ID SFA308P
Connected seq. 
>SFA308P.Seq
ACTGTTGGCCTACTGGCATTACTTTATTTTTTTTTATCTCTATCTATATATATATCATTT
TTTAAAATGAGCGTCATCAAATCAATTAAAGCCAGAGGAAATTTTAGATTCACGTGGTAA
CCCAACTGTTGAAGTTGACCTTTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGC
TGCAGTTCCATCAGGTGCATCAACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGA
TAAATCAAGATACTTAGGTAAAGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCAT
TCAACCAGCCGTCATTGGTAAATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGAT
CGATCTCGATGGTACCCCAAATAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAG
TTTAGCTGTTTGCAGAGCTGGTGCTGCTGATAGAAATTTACCACTTTACAAATATATCTC
AGAAATTGCAGGTACTAAAATGAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTG-
---------TTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAAT
GGTTACGATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAA
AAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGAT
CCATTTGATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAA
ATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAG
AAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACT
Length of connected seq. 881
Full length Seq ID -
Full length Seq. -
Length of full length seq. -