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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFA258
Library SF
(Link to library)
Clone ID SFA258
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09871-1
Original site URL
Representative seq. ID SFA258P
(Link to Original site)
Representative DNA sequence 
>SFA258 (SFA258Q) /CSM/SF/SFA2-C/SFA258Q.Seq.d/
ACTGTTGGCCTACTGGAACATTTCATTCATATATTTGTATATACTTATATAAAATGTCAC
TCTTTAATTCAGCTAATAAAGTTATTGGAGGAGTTTTAAAATTTAATCCAAGTAAATATC
AAGTTAGATATTTAAATTTACATGAATATCAAAGTAAATCATTAATGGATAAATATGGAG
TTAATACACAAAAATGGAGAGTTGTAACAAAAGCATCAGATGCCATCAAAGCAGCATCAG
AATTGAATGGAGAATTAGTAGTTAAAGCACAAGTACATGCAGGTGGTAGAGGTAAGGGTT
CATTCATAGAGACAGGATTTAAAGGAGGTGTTCATXXXXXXXXXXTGGTAACATTGGTTG
TATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAATTAAAGGGTGG
TATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAGC
CTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTGG
TATCATGAAATGTGATATCATTGCCTCCTGGTATCGTAAATGCTTCAAAACAAGTTGGTC
TTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGTAAAGAAATCC
TTGGAAAAATNCTGGTTTAAATATTACTTCTGCTAGTGATTTAGATGATGCTGCTATTAA
AGCTGTTAATTGTTTAAAAAAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
CWPTGTFHSYICIYLYKMSLFNSANKVIGGVLKFNPSKYQVRYLNLHEYQSKSLMDKYGV
NTQKWRVVTKASDAIKAASELNGELVVKAQVHAGGRGKGSFIETGFKGGVH---

---GNIGCMVNGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKC
LLVNIFGGIMKCDIIASWYRKCFKTSWS*nsisctfrrykckhw*rnpwknxglnitsas
dlddaaikavnclkk
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllehfihifvytyikchsliqliklleef*nliqvnikldi*iymnikvnh*winme
lihkngel*qkhqmpskqhqn*men**lkhkymqvvevrvhs*rqdlkevf---

---w*hwlyg*wcwfgyghhgyh*ikgwypc*fprcrw*cl*tsrh*slqnph*rsks*m
ftrkhfrwyhem*yhcllvs*mlqnklvlkfh*lyv*kvqm*tlvkkslekxwfkyyfc*
*fr*ccy*sc*lfkki

Frame B:
llaywnisfiylyili*nvtl*fs**sywrsfki*sk*iss*ifkft*isk*iing*iws
*ytkmescnksirchqssiriewriss*ststcrw*r*gfihrdri*rrcs---

---GNIGCMVNGAGLAMATMDIIKLKGGIPANFLDVGGSASEQAVTEAFKILTKDPRVKC
LLVNIFGGIMKCDIIASWYRKCFKTSWS*nsisctfrrykckhw*rnpwknxglnitsas
dlddaaikavnclkk

Frame C:
CWPTGTFHSYICIYLYKMSLFNSANKVIGGVLKFNPSKYQVRYLNLHEYQSKSLMDKYGV
NTQKWRVVTKASDAIKAASELNGELVVKAQVHAGGRGKGSFIETGFKGGVH---

---vtlvvwlmvlvwlwppwisln*rvvsllissm*vvvplnkpslkpsksslkiqelnv
ys*tfsvvs*nvislppgivnaskqvglkiplvvrlegtnvnigkeilgkxlv*illllv
i*mmlllklliv*kn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA258 (SFA258Q) /CSM/SF/SFA2-C/SFA258Q.Seq.d/                       1392   0.0
VFN263 (VFN263Q) /CSM/VF/VFN2-C/VFN263Q.Seq.d/                        726   0.0
SSI167 (SSI167Q) /CSM/SS/SSI1-C/SSI167Q.Seq.d/                        726   0.0
SLG890 (SLG890Q) /CSM/SL/SLG8-D/SLG890Q.Seq.d/                        726   0.0
SLG726 (SLG726Q) /CSM/SL/SLG7-B/SLG726Q.Seq.d/                        726   0.0
AFB401 (AFB401Q) /CSM/AF/AFB4-A/AFB401Q.Seq.d/                        726   0.0
AFD423 (AFD423Q) /CSM/AF/AFD4-A/AFD423Q.Seq.d/                        724   0.0
VFH579 (VFH579Q) /CSM/VF/VFH5-D/VFH579Q.Seq.d/                        720   0.0
VFG281 (VFG281Q) /CSM/VF/VFG2-D/VFG281Q.Seq.d/                        716   0.0
VFM565 (VFM565Q) /CSM/VF/VFM5-C/VFM565Q.Seq.d/                        712   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   494   0.0    10
AF493055|AF493055.1 Neocallimastix patriciarum succinyl-CoA synthetase, beta subunit (scsB) mRNA, complete cds.    74   8e-19  2
X84222|X84222.2 Neocallimastix frontalis mRNA for beta-succinyl CoA synthetase  precursor.    74   3e-18  2
AX621832|AX621832.1 Sequence 4795 from Patent WO02094868.          50   2e-17  4
AR354123|AR354123.1 Sequence 241 from patent US 6593114.           50   1e-15  4
Y11777|Y11777.1 R.prowazekii rbfA, sucC, sucD, com1, smpB, dapA, ilvE genes.    70   3e-15  2
BJ519880|BJ519880.1 Oryzias latipes cDNA clone:MF01SSB014A03, 5' end single read.    66   4e-15  2
BJ532800|BJ532800.1 Oryzias latipes cDNA clone:MF01SSB015A03, 3' end single read.    66   4e-15  2
BJ520215|BJ520215.1 Oryzias latipes cDNA clone:MF01SSB015A03, 5' end single read.    66   4e-15  2
AL034491|AL034491.1 S.pombe chromosome III cosmid c1620.           70   2e-14  2
dna update 2003.12.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q2IIC1) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   120   2e-35
CP001131_2217(CP001131|pid:none) Anaeromyxobacter sp. K, complet...   120   4e-35
(Q5L0N7) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   120   7e-35
(A8MM16) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   120   2e-34
CP001390_241(CP001390|pid:none) Geobacter sp. FRC-32, complete g...   115   3e-33
CP000903_3561(CP000903|pid:none) Bacillus weihenstephanensis KBA...   111   2e-32
CP001176_3782(CP001176|pid:none) Bacillus cereus B4264, complete...   111   2e-32
(A0RHK9) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   111   2e-32
(Q819X0) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   111   2e-32
CP000698_4274(CP000698|pid:none) Geobacter uraniireducens Rf4, c...   114   4e-32
protein update 2009. 5.21
PSORT 

 psg: 0.86  gvh: 0.37  alm: 0.33  top: 0.57  tms: 0.00  mit: 0.30  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 20.0 %: cytoskeletal
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for SFA258 is cyt
5' end seq. ID SFA258F
5' end seq. 
>SFA258F.Seq
ACTGTTGGCCTACTGGAACATTTCATTCATATATTTGTATATACTTATATAAAATGTCAC
TCTTTAATTCAGCTAATAAAGTTATTGGAGGAGTTTTAAAATTTAATCCAAGTAAATATC
AAGTTAGATATTTAAATTTACATGAATATCAAAGTAAATCATTAATGGATAAATATGGAG
TTAATACACAAAAATGGAGAGTTGTAACAAAAGCATCAGATGCCATCAAAGCAGCATCAG
AATTGAATGGAGAATTAGTAGTTAAAGCACAAGTACATGCAGGTGGTAGAGGTAAGGGTT
CATTCATAGAGACAGGATTTAAAGGAGGTGTTCAT----------
Length of 5' end seq. 335
3' end seq. ID SFA258Z
3' end seq. 
>SFA258Z.Seq
----------TGGTAACATTGGTTGTATGGTTAATGGTGCTGGTTTGGCTATGGCCACCA
TGGATATCATTAAATTAAAGGGTGGTATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTG
CCTCTGAACAAGCCGTCACTGAAGCCTTCAAAATCCTCACTAAAGATCCAAGAGTTAAAT
GTTTACTCGTAAACATTTTCGGTGGTATCATGAAATGTGATATCATTGCCTCCTGGTATC
GTAAATGCTTCAAAACAAGTTGGTCTTAAAATTCCATTAGTTGTACGTTTAGAAGGTACA
AATGTAAACATTGGTAAAGAAATCCTTGGAAAAATNCTGGTTTAAATATTACTTCTGCTA
GTGATTTAGATGATGCTGCTATTAAAGCTGTTAATTGTTTAAAAAAATA
Length of 3' end seq. 399
Connected seq. ID SFA258P
Connected seq. 
>SFA258P.Seq
ACTGTTGGCCTACTGGAACATTTCATTCATATATTTGTATATACTTATATAAAATGTCAC
TCTTTAATTCAGCTAATAAAGTTATTGGAGGAGTTTTAAAATTTAATCCAAGTAAATATC
AAGTTAGATATTTAAATTTACATGAATATCAAAGTAAATCATTAATGGATAAATATGGAG
TTAATACACAAAAATGGAGAGTTGTAACAAAAGCATCAGATGCCATCAAAGCAGCATCAG
AATTGAATGGAGAATTAGTAGTTAAAGCACAAGTACATGCAGGTGGTAGAGGTAAGGGTT
CATTCATAGAGACAGGATTTAAAGGAGGTGTTCAT----------TGGTAACATTGGTTG
TATGGTTAATGGTGCTGGTTTGGCTATGGCCACCATGGATATCATTAAATTAAAGGGTGG
TATCCCTGCTAATTTCCTCGATGTAGGTGGTAGTGCCTCTGAACAAGCCGTCACTGAAGC
CTTCAAAATCCTCACTAAAGATCCAAGAGTTAAATGTTTACTCGTAAACATTTTCGGTGG
TATCATGAAATGTGATATCATTGCCTCCTGGTATCGTAAATGCTTCAAAACAAGTTGGTC
TTAAAATTCCATTAGTTGTACGTTTAGAAGGTACAAATGTAAACATTGGTAAAGAAATCC
TTGGAAAAATNCTGGTTTAAATATTACTTCTGCTAGTGATTTAGATGATGCTGCTATTAA
AGCTGTTAATTGTTTAAAAAAATA
Length of connected seq. 734
Full length Seq ID -
Full length Seq. -
Length of full length seq. -