>SFA227 (SFA227Q) /CSM/SF/SFA2-B/SFA227Q.Seq.d/
CACCACCACGTCCACCAGTTGACCAATGCCGTAATCAACATTGTCCACATGGTTATTCAT
GTCGTGTCATTAAAGGTTGTGCCACTTGTGTCAGAGATGCCCGTCCACCACATAACCTTT
GTCGTGGTTTTGGTTGTCCAGAAGGCTCCCATTGTGAAGTTCTTGAAAAACATCCAGTTT
GTGTTAGAAATTATGTTCCACCACACCCACCACCACCACCACAAATTTGCGGTAGTGTAA
ATTGTGGTCCAGGTTATATTTGTACAATTATCAATGGTCATCCAACTTGNATTCGTGGTG
ATGGTTATTTATGTAATCAAACCCGTTXXXXXXXXXXGTCCACACTGTCTTGCCAATAAT
GTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATGATT
AGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCAACT
TGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGC
AATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCA
ACTACTGCTGCTTCAACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCA
ACTACTGCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGT
GCTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCA
AGTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCT
TCAAGTAGCGCTCCATCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCA
GCTGCATCATCAGCTGCAAGCNCAGAAAG

PPRPPVDQCRNQHCPHGYSCRVIKGCATCVRDARPPHNLCRGFGCPEGSHCEVLEKHPVC
VRNYVPPHPPPPPQICGSVNCGPGYICTIINGHPTXIRGDGYLCNQTR---

---PHCLANNVFPQLCKVTQCPTDFSCKMIRGNPTCIKARPPVPPPHCSTCAELSSACNH
VGMICIQVPSNCTNTRFPCCPSHPICIHPSTTAASTIATTASTVATTTSATTAGTTTGGT
TTGGSTSDSSAASSADSSAASSSPSSSAASSAASSEPSSSAASSSAPSSASSSAPSSASS
SAPSSSASSSAASSAASXE

Frame A:
hhhvhqltnavinivhmvihvvslkvvplvsempvhhitfvvvlvvqkapivkflkniqf
vleimfhhthhhhhkfavv*ivvqvifvqlsmviqlxfvvmviyvikpv---

---vhtvlpimyshnfvkllnvqlislvk*leviqlvlkqdhryhhhiaqlvqsyhqhvi
tle*fvfkyqaivpildshvahliqfvfihqllllqplqqlhqlsqlqplqllqvqqlve
lqlvvqllivvllpqpivalpphhhqavllqvplqvnhqvaplqvvlhpllqvalhplpq
vvlhhhqlqvqlhhqlqaqk

Frame B:
tttsts*pmp*stlstwlfmsch*rlchlcqrcpstt*plswfwlsrrlpl*ss*ktssl
c*klcsttptttttnlr*cklwsrlylynyqwssnlxsw*wlfm*snp---

---stlscq*cipttl*sysmsn*fll*nd*r*snly*skttgttttllnlcraiism*s
rwndlysstkqlyqy*ipmlpissnlyssinyccfnhcnncincrnynlcnycrynnwwn
ynwwfnf***ccflsr**rcllitikqccfkcrfk*tik*rrfk*csilcfk*rsilclk
*csiiisfkfsciisckxrk

Frame C:
PPRPPVDQCRNQHCPHGYSCRVIKGCATCVRDARPPHNLCRGFGCPEGSHCEVLEKHPVC
VRNYVPPHPPPPPQICGSVNCGPGYICTIINGHPTXIRGDGYLCNQTR---

---PHCLANNVFPQLCKVTQCPTDFSCKMIRGNPTCIKARPPVPPPHCSTCAELSSACNH
VGMICIQVPSNCTNTRFPCCPSHPICIHPSTTAASTIATTASTVATTTSATTAGTTTGGT
TTGGSTSDSSAASSADSSAASSSPSSSAASSAASSEPSSSAASSSAPSSASSSAPSSASS
SAPSSSASSSAASSAASXE

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSG603 (SSG603Q) /CSM/SS/SSG6-A/SSG603Q.Seq.d/                       1168   0.0
SLG750 (SLG750Q) /CSM/SL/SLG7-C/SLG750Q.Seq.d/                       1168   0.0
SLE334 (SLE334Q) /CSM/SL/SLE3-B/SLE334Q.Seq.d/                       1168   0.0
SLE302 (SLE302Q) /CSM/SL/SLE3-A/SLE302Q.Seq.d/                       1168   0.0
SLE241 (SLE241Q) /CSM/SL/SLE2-B/SLE241Q.Seq.d/                       1168   0.0
SLC119 (SLC119Q) /CSM/SL/SLC1-A/SLC119Q.Seq.d/                       1168   0.0
SLB706 (SLB706Q) /CSM/SL/SLB7-A/SLB706Q.Seq.d/                       1168   0.0
SLB313 (SLB313Q) /CSM/SL/SLB3-A/SLB313Q.Seq.d/                       1168   0.0
SLB244 (SLB244Q) /CSM/SL/SLB2-B/SLB244Q.Seq.d/                       1168   0.0
SLB211 (SLB211Q) /CSM/SL/SLB2-A/SLB211Q.Seq.d/                       1168   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.  1168   0.0    4
BJ170760|BJ170760.1 Physcomitrella patens subsp. patens cDNA clone:pph26m16, 3' end, single read.   190   2e-44  1
AF279135|AF279135.1 Dictyostelium discoideum spore coat structural protein SP65 (cotE) gene, complete cds.    64   4e-06  1
C26876|C26876.1 Oryza sativa (japonica cultivar-group) cDNA, partial sequence (C50317_1A).    36   0.40   2
CD069679|CD069679.1 MA2-0011T-V070-E04-U.B MA2-0011 Schistosoma mansoni cDNA clone MA2-0011T-V070-E04.B, mRNA sequence.    34   2.2    2
CD069686|CD069686.1 MA2-0011T-V070-E11-U.B MA2-0011 Schistosoma mansoni cDNA clone MA2-0011T-V070-E11.B, mRNA sequence.    34   2.2    2
AK100095|AK100095.1 Oryza sativa (japonica cultivar-group) cDNA clone:J023004B12, full insert sequence.    36   2.4    2
AY344488|AY344488.1 Oryza sativa (japonica cultivar-group) heat shock factor RHSF6 mRNA, complete cds.    44   3.4    1
AK072391|AK072391.1 Oryza sativa (japonica cultivar-group) cDNA clone:J023077L03, full insert sequence.    44   3.4    1
BX546501|BX546501.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone BUSM1-216C6.    44   3.4    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       228   3e-58
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   228   3e-58
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    70   9e-13
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    61   2e-09
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    61   2e-09
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    52   1e-08
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    50   7e-08
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    47   2e-06
S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...    47   9e-06
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    49   2e-04

 psg: 0.74  gvh: 0.53  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for SFA227 is mit

>SFA227F.Seq
CACCACCACGTCCACCAGTTGACCAATGCCGTAATCAACATTGTCCACATGGTTATTCAT
GTCGTGTCATTAAAGGTTGTGCCACTTGTGTCAGAGATGCCCGTCCACCACATAACCTTT
GTCGTGGTTTTGGTTGTCCAGAAGGCTCCCATTGTGAAGTTCTTGAAAAACATCCAGTTT
GTGTTAGAAATTATGTTCCACCACACCCACCACCACCACCACAAATTTGCGGTAGTGTAA
ATTGTGGTCCAGGTTATATTTGTACAATTATCAATGGTCATCCAACTTGNATTCGTGGTG
ATGGTTATTTATGTAATCAAACCCGTT----------

>SFA227Z.Seq
----------GTCCACACTGTCTTGCCAATAATGTATTCCCACAACTTTGTAAAGTTACT
CAATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACTTGTATTAAAGCA
AGACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCATCAGCATGTAAT
CACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGATTCCCATGT
TGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCATTGCAACA
ACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTGGA
ACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCT
GCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGT
AGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCTCA
AGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAGCNCAGAA
AG

>SFA227P.Seq
CACCACCACGTCCACCAGTTGACCAATGCCGTAATCAACATTGTCCACATGGTTATTCAT
GTCGTGTCATTAAAGGTTGTGCCACTTGTGTCAGAGATGCCCGTCCACCACATAACCTTT
GTCGTGGTTTTGGTTGTCCAGAAGGCTCCCATTGTGAAGTTCTTGAAAAACATCCAGTTT
GTGTTAGAAATTATGTTCCACCACACCCACCACCACCACCACAAATTTGCGGTAGTGTAA
ATTGTGGTCCAGGTTATATTTGTACAATTATCAATGGTCATCCAACTTGNATTCGTGGTG
ATGGTTATTTATGTAATCAAACCCGTT----------GTCCACACTGTCTTGCCAATAAT
GTATTCCCACAACTTTGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATGATT
AGAGGTAATCCAACTTGTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCAACT
TGTGCAGAGCTATCATCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGC
AATTGTACCAATACTAGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCA
ACTACTGCTGCTTCAACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCA
ACTACTGCAGGTACAACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGT
GCTGCTTCCTCAGCCGATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCA
AGTGCCGCTTCAAGTGAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCT
TCAAGTAGCGCTCCATCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCA
GCTGCATCATCAGCTGCAAGCNCAGAAAG