NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFA148
Library SF
(Link to library)
Clone ID SFA148
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16560-1
Original site URL
Representative seq. ID SFA148Z
(Link to Original site)
Representative DNA sequence 
>SFA148 (SFA148Q) /CSM/SF/SFA1-B/SFA148Q.Seq.d/
XXXXXXXXXXTCCAANATAAANAAGGTTTCCCCCCAGNTCAACAANGTTTTTTTTTTGGG
GGGAAACAATTAAAAAAATGGTCGTTCTCTTTCCGNNCTATAACATTCAAAAGGNATCCC
CCTCNCCCNCTTAGTTTAAANATTAAAAGGTGGTATGCAAATCTTTGTTAAAACTCNCCN
CTGGTAAAACAATCCCTTTANAAGTTGAAGGTTTNTGATACCTTGAAAAATGTAAAGGCT
AAAATTCAAGATAAAGAAGGTTTTCCACCAGATCAACAACGTCTCATTTTTGCTGGTAAA
CAATTGNAANATGGTCGTNCTCTCTCTGACTATAACATTCAAAAGGAATCANCTCTTCNC
TTAGTTTTAANATTAANAGGTGGTATGCAAATNTTTGTTAAAACTCTCACTGGTAAAACT
ATCCCTTTGGAAGTTGAAGGTTCTGATAATATTGAAAATGTAAAGGCTAAAATCCAANAT
AAAGAAGGTATTCCNCCAGATCAACAAAGATTAATNTTTGCCGGTAAACAATTANAANAT
GGTCGTACTCTTTCTGACTACAATATTCAAAAAGAATCCCCTCTCCATTTAGTTTTAAGA
TTAANAGGTGGTATGCAAATCTTTGTTAAAACTCTCACTGGTAAAACAATCCCTTTANAA
GTTGAAGGTTCTGATAACATTGAAAATGTTAAAGCCAAGATCCAAGATAAAGAAGGTATT
CCNCCAGATCAACAAAGATTAATTCTTTGCCGGTAAACAATTAGAAGATGGTCGNACTC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---qxkxgfppxqqxfffggkqlkkwsfsfrxitfkrxppxplslxikrwyanlc*NSPL
VKQSLXKLKVXDTLKNVKAKIQDKEGFPPDQQRLIFAGKQLXXGRXLSDYNIQKESXLXL
VLXLXGGMQXFVKTLTGKTIPLEVEGSDNIENVKAKIQXKEGIPPDQQRLXFAGKQLXXG
RTLSDYNIQKESPLHLVLRLXGGMQIFVKTLTGKTIPLXVEGSDNIENVKAKIQDKEGIP
PDQQRLILCR*tirrwsx
Translated Amino Acid sequence (All Frames) 
Frame A:
---px*xrfppxstxfffwgetikkmvvlfpxyniqkxspspx*fkx*kvvcksllklxx
gktiplxvegx*ylekckg*nsr*rrfstrsttshfcw*tixxwsxsl*l*hskgixssl
sfxixrwyanxc*nshw*nypfgs*rf**y*kckg*npx*rrysxrstkinxcr*tixxw
sysf*lqyskripspfsfkixrwyanlc*nshw*nnpfxs*rf**h*kc*sqdpr*rrys
xrstkinslpvnn*kmvxl


Frame B:
---qxkxgfppxqqxfffggkqlkkwsfsfrxitfkrxppxplslxikrwyanlc*NSPL
VKQSLXKLKVXDTLKNVKAKIQDKEGFPPDQQRLIFAGKQLXXGRXLSDYNIQKESXLXL
VLXLXGGMQXFVKTLTGKTIPLEVEGSDNIENVKAKIQXKEGIPPDQQRLXFAGKQLXXG
RTLSDYNIQKESPLHLVLRLXGGMQIFVKTLTGKTIPLXVEGSDNIENVKAKIQDKEGIP
PDQQRLILCR*tirrwsx


Frame C:
---xixkvspqxnxvfflggnn*kngrslsxl*hskgiplxxlv*xlkggmqifvktxxw
*nnpfxs*rfxip*km*rlkfkikkvfhqinnvsfllvnnxxmvvlsltitfkrnxlfx*
f*x*xvvckxllklslvklslwklkvliilkm*rlksxikkvfxqinkd*xlpvnnxxmv
vlflttifkknplsi*f*d*xvvcksllklslvkqslxklkvlitlkmlkprskikkvfx
qinkd*ffagkqledgrt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA148 (SFA148Q) /CSM/SF/SFA1-B/SFA148Q.Seq.d/                       1239   0.0
SFF654 (SFF654Q) /CSM/SF/SFF6-C/SFF654Q.Seq.d/                       1047   0.0
VFK831 (VFK831Q) /CSM/VF/VFK8-B/VFK831Q.Seq.d/                       1037   0.0
AFO288 (AFO288Q) /CSM/AF/AFO2-D/AFO288Q.Seq.d/                       1029   0.0
SFH395 (SFH395Q) /CSM/SF/SFH3-D/SFH395Q.Seq.d/                       1019   0.0
CFF694 (CFF694Q) /CSM/CF/CFF6-D/CFF694Q.Seq.d/                       1003   0.0
CHB665 (CHB665Q) /CSM/CH/CHB6-C/CHB665Q.Seq.d/                        997   0.0
CFJ877 (CFJ877Q) /CSM/CF/CFJ8-D/CFJ877Q.Seq.d/                        997   0.0
CFK434 (CFK434Q) /CSM/CF/CFK4-B/CFK434Q.Seq.d/                        993   0.0
CHE242 (CHE242Q) /CSM/CH/CHE2-B/CHE242Q.Seq.d/                        991   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M23754|M23754.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB2.   898   0.0    5
M23752|M23752.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB17.   708   0.0    4
M23751|M23751.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB18.   708   0.0    4
M19492|M19492.1 Slime mold (D.discoideum) ubiquitin lambda-229 gene, complete cds.   444   0.0    5
M23753|M23753.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB14.   551   e-169  4
M19491|M19491.1 Slime mold (D.discoideum) ubiquitin p229C/pLK229 gene, complete cds.   222   e-140  6
M23748|M23748.1 D.discoideum ubiquitin mRNA, complete cds, clone DCUB19.   216   e-139  6
M19666|M19666.1 Slime mold (D.discoideum) ubiquitin gene, 3' end, clone p229C.   216   e-138  6
CF181989|CF181989.1 ISO4G3F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   359   e-115  4
CF181994|CF181994.1 ISO4G8F Iso cDNA Isotricha sp. BBF-2003 cDNA similar to ubiquitin, mRNA sequence.   359   e-115  4
dna update 2003.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M23754_1(M23754|pid:none) D.discoideum ubiquitin mRNA, complete ...   298   4e-83
A27806(A27806) polyubiquitin 5 (clone pLK229) - slime mold (Dict...   295   3e-82
M19666_1(M19666|pid:none) Slime mold (D.discoideum) ubiquitin ge...   295   3e-82
B34080(B34080)polyubiquitin 5 (clone DCUB19) - slime mold (Dicty...   295   3e-82
B27806(B27806) ubiquitin (clone lambda229) - slime mold (Dictyos...   298   7e-81
FN315483_1(FN315483|pid:none) Schistosoma japonicum isolate Anhu...   289   5e-80
AE017351_259(AE017351|pid:none) Cryptococcus neoformans var. neo...   288   5e-80
AF060501_1(AF060501|pid:none) Botryotinia fuckeliana polyubiquit...   288   5e-80
AB021974_1(AB021974|pid:none) Bombyx mori UBI3 mRNA for polyubiq...   291   5e-80
EF134146_1(EF134146|pid:none) Karlodinium micrum strain CCMP1975...   286   1e-79
protein update 2009. 5.21
PSORT 

 psg: 0.57  gvh: 0.38  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 1.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for SFA148 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID SFA148Z
3' end seq. 
>SFA148Z.Seq
----------TCCAANATAAANAAGGTTTCCCCCCAGNTCAACAANGTTTTTTTTTTGGG
GGGAAACAATTAAAAAAATGGTCGTTCTCTTTCCGNNCTATAACATTCAAAAGGNATCCC
CCTCNCCCNCTTAGTTTAAANATTAAAAGGTGGTATGCAAATCTTTGTTAAAACTCNCCN
CTGGTAAAACAATCCCTTTANAAGTTGAAGGTTTNTGATACCTTGAAAAATGTAAAGGCT
AAAATTCAAGATAAAGAAGGTTTTCCACCAGATCAACAACGTCTCATTTTTGCTGGTAAA
CAATTGNAANATGGTCGTNCTCTCTCTGACTATAACATTCAAAAGGAATCANCTCTTCNC
TTAGTTTTAANATTAANAGGTGGTATGCAAATNTTTGTTAAAACTCTCACTGGTAAAACT
ATCCCTTTGGAAGTTGAAGGTTCTGATAATATTGAAAATGTAAAGGCTAAAATCCAANAT
AAAGAAGGTATTCCNCCAGATCAACAAAGATTAATNTTTGCCGGTAAACAATTANAANAT
GGTCGTACTCTTTCTGACTACAATATTCAAAAAGAATCCCCTCTCCATTTAGTTTTAAGA
TTAANAGGTGGTATGCAAATCTTTGTTAAAACTCTCACTGGTAAAACAATCCCTTTANAA
GTTGAAGGTTCTGATAACATTGAAAATGTTAAAGCCAAGATCCAAGATAAAGAAGGTATT
CCNCCAGATCAACAAAGATTAATTCTTTGCCGGTAAACAATTAGAAGATGGTCGNACTC
Length of 3' end seq. 769
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -