>AFH766 (AFH766Q) /CSM/AF/AFH7-C/AFH766Q.Seq.d/
XXXXXXXXXXTAACACTCAAGTTCTAGAAGATGTAAAGAAAATCGCTACCTCACAAAAAG
GTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGG
GTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTT
ACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATG
TCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATG
CTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGG
ATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCT
ACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATG
CCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCT
TCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGA
ACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAA
CTGATGATAATGTGAATCTAAAAAAGATTCTTCTGCTAAACANANAATTA

---NTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARY
NFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKD
VLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLF
KADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKKILLLNXXL

Frame A:
---NTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARY
NFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKD
VLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLF
KADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTDDNVNLKKILLLNXXL


Frame B:
---tlkf*km*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvt
ismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrm
fwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscss
klmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklmim*i*krffc*txn


Frame C:
---hsssrrckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslq
fr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgc
fg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalq
s**qrksnll*rskk*syelc*ihsr*rch*iqitiisn***ceskkdssakxxi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH766 (AFH766Q) /CSM/AF/AFH7-C/AFH766Q.Seq.d/                       1376   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1368   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1364   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1362   0.0
AFN128 (AFN128Q) /CSM/AF/AFN1-B/AFN128Q.Seq.d/                       1362   0.0
AFM768 (AFM768Q) /CSM/AF/AFM7-C/AFM768Q.Seq.d/                       1362   0.0
AFM249 (AFM249Q) /CSM/AF/AFM2-C/AFM249Q.Seq.d/                       1362   0.0
AFL563 (AFL563Q) /CSM/AF/AFL5-C/AFL563Q.Seq.d/                       1362   0.0
AFO479 (AFO479Q) /CSM/AF/AFO4-D/AFO479Q.Seq.d/                       1360   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1358   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   2e-10  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   3e-09  1
CK029548|CK029548.1 AGENCOURT_16620510 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7051751 5', mRNA sequence.    70   5e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70   5e-08  1
CN206834|CN206834.1 Tor7258 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    70   5e-08  1
CK030146|CK030146.1 AGENCOURT_16621384 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7052631 5', mRNA sequence.    70   5e-08  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   173   5e-42
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   173   5e-42
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   170   4e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   168   2e-40
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   163   4e-39
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   163   5e-39
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   3e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   3e-35

 psg: 0.65  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system

>> prediction for AFH766 is cyt

>AFH766Z.Seq
----------TAACACTCAAGTTCTAGAAGATGTAAAGAAAATCGCTACCTCACAAAAAG
GTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGG
GTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTT
ACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATG
TCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATG
CTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGG
ATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCT
ACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATG
CCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCT
TCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGA
ACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAA
CTGATGATAATGTGAATCTAAAAAAGATTCTTCTGCTAAACANANAATTA