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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
SFA112
Library SF
(Link to library)
Clone ID SFA112
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16453-1
Original site URL
Representative seq. ID SFA112P
(Link to Original site)
Representative DNA sequence 
>SFA112 (SFA112Q) /CSM/SF/SFA1-A/SFA112Q.Seq.d/
ACTGTTGGCCTACTGGTTAAAATTTAAATCACAAACGAAAGAAATAAAACATGTCCGCAA
GTAATAAAGATGACCAACTCATGAAAAATGAGTTCGAAAGTACCTACGACAAAATTGTCG
ATTCATTCGACAATATGGAGCTCCCAAAGGATCTCTTAAGAGGTATCTACTCCTATGGTT
TTGAAAAGCCATCAGCCATCCAACAAAGAGCTATCATTCCACTCTCAAAAGGTTTTGATA
TTATTGCCCAAGCTCAATCTGGTACTGGTAAAACTGCTACCTTCACCGTTGGTATCTTAT
CTAGAATTGATATTTCAGTCCTCAAATGTCAAGCTCTCATCATTGCCCCAACTCGTGAAC
TTGCTCAACAAATTCAAAAGGTTGTTTTAGCTTTAGGTGAATATTTACACATTAAATCAT
ACGCATGTATTGGTGGTACTAAGATTACTGATGATATTCAAGCCCTCCAAAGTGGTGTAC
ACGTAGTTGTCGGTACCCCAGGTCGTGTCTTTGAAATGTTATCACGTGGTATCATTAATC
GNGACACCATTAGAATTTTCTGTCTTGATGAAGCTGATGAAATGTTATXXXXXXXXXXCG
TCAAAAAGGATGAATTAACCCTTGAAGGTATTAGACAATTCTACGTCAACGTTGGTGTTG
AACAAGGTAAATTTGAAGTTTTAACCGATCTCTACGAAACCCTCTCTATCACTCAATCCG
TTATTTTCTGTAACACCCGTAGAAAGGTCGATTGGTTAACAAGCAAAATGACCGAACAAA
AATTCACCGTCTCATCAACTCACGGTGACCAAAAGGATCGTGATGGTATCTTACAAGCTT
TCCGTTCTGGTGCTACTCGTGTCCTCATCACCACCGATCTCTTAGCTCGTGGTATTGATG
TCCAACAAGTATCACTCGTTATCAACTTTGATTTACCAACCAACATTGAAAATTACATTC
ATCGTATCGGTCGTTCAGGTCGTTTCGGTCGTAAGGGTGTTGCCATCAACTTTATCACTG
ACGCTGAAAGAGGTCAAATCGATGAATTATNCTCGTCATTACAATACCTACATTGCCGAA
ATGCCACAAGACATTGGAAGTTTCCTCTAAACGAGTTCCAAAAATGGTTTATAGTTCAGT
TGGTCCCCATTTGGTGAGGGCCATAGGGTTTATAAATCGACCGCCCCCCTCCCTATCCAA
TACTCACACCACAACAACATATTTCAACCGNCTNCATTCATAAAAACAACAAAAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
cwptg*nlnhkrkk*NMSASNKDDQLMKNEFESTYDKIVDSFDNMELPKDLLRGIYSYGF
EKPSAIQQRAIIPLSKGFDIIAQAQSGTGKTATFTVGILSRIDISVLKCQALIIAPTREL
AQQIQKVVLALGEYLHIKSYACIGGTKITDDIQALQSGVHVVVGTPGRVFEMLSRGIINR
DTIRIFCLDEADEML---

---VKKDELTLEGIRQFYVNVGVEQGKFEVLTDLYETLSITQSVIFCNTRRKVDWLTSKM
TEQKFTVSSTHGDQKDRDGILQAFRSGATRVLITTDLLARGIDVQQVSLVINFDLPTNIE
NYIHRIGRSGRFGRKGVAINFITDAERGQIDELXSSLQYLHCRNATRHWKFPLNEFQKWF
IVQLVPIW*gp*gl*idrpppypiltpqqhistxxihknnk
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllvki*itnernktcpqvikmtns*kmsskvpttklsihstiwssqris*evstpmv
lkshqpsnkelsfhsqkvlillpklnlvlvkllpsplvsylelifqssnvklsslpqlvn
llnkfkrlf*l*vniytlnhthvlvvlrllmifkpskvvyt*lsvpqvvslkcyhvvsli
xtplefsvlmklmkcy---

---rqkg*inp*ry*tilrqrwc*tr*i*sfnrslrnplyhsiryfl*hp*kgrlvnkqn
drtkihrlinsr*pkgs*wyltsfpfwcyscphhhrslsswy*cptsitryql*ftnqh*
klhssyrsfrsfrs*gcchqlyh*r*krsnr*ixlvitiptlpkchktlevsskrvpkmv
yssvgphlvraigfinrpppslsnthttttyfnrlhs*kqqk

Frame B:
llaywlkfksqtkeikhvrk**r*pthek*vrkylrqncrfirqygapkgslkrylllwf
*kaishptksyhstlkrf*yycpssiwyw*ncylhrwyli*n*yfspqmssshhcpns*t
cstnskgcfsfr*ifth*iirmywwy*dy**yssppkwctrscryprscl*nvitwyh*s
xhh*nfls**s**nv---

---VKKDELTLEGIRQFYVNVGVEQGKFEVLTDLYETLSITQSVIFCNTRRKVDWLTSKM
TEQKFTVSSTHGDQKDRDGILQAFRSGATRVLITTDLLARGIDVQQVSLVINFDLPTNIE
NYIHRIGRSGRFGRKGVAINFITDAERGQIDELXSSLQYLHCRNATRHWKFPLNEFQKWF
IVQLVPIW*gp*gl*idrpppypiltpqqhistxxihknnk

Frame C:
cwptg*nlnhkrkk*NMSASNKDDQLMKNEFESTYDKIVDSFDNMELPKDLLRGIYSYGF
EKPSAIQQRAIIPLSKGFDIIAQAQSGTGKTATFTVGILSRIDISVLKCQALIIAPTREL
AQQIQKVVLALGEYLHIKSYACIGGTKITDDIQALQSGVHVVVGTPGRVFEMLSRGIINR
DTIRIFCLDEADEML---

---skrmn*plkvldnststlvlnkvnlkf*pistkpslslnplfsvtpversig*qak*
pnknspshqltvtkrivmvsyklsvlvllvsssppis*lvvlmsnkyhslstliyqptlk
itfivsvvqvvsvvrvlpstlsltlkevksmnyxrhyntyiaempqdigsfl*tsskngl
*fswspfgeghrvykstaplpiqyshhnnifqpxxfikttk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFA112 (SFA112Q) /CSM/SF/SFA1-A/SFA112Q.Seq.d/                       2405   0.0
SFK738 (SFK738Q) /CSM/SF/SFK7-B/SFK738Q.Seq.d/                       1241   0.0
VFH760 (VFH760Q) /CSM/VF/VFH7-C/VFH760Q.Seq.d/                       1235   0.0
VFI417 (VFI417Q) /CSM/VF/VFI4-A/VFI417Q.Seq.d/                       1231   0.0
VFI122 (VFI122Q) /CSM/VF/VFI1-A/VFI122Q.Seq.d/                       1231   0.0
VFF484 (VFF484Q) /CSM/VF/VFF4-D/VFF484Q.Seq.d/                       1231   0.0
VFD768 (VFD768Q) /CSM/VF/VFD7-C/VFD768Q.Seq.d/                       1231   0.0
VFB309 (VFB309Q) /CSM/VF/VFB3-A/VFB309Q.Seq.d/                       1231   0.0
SLE749 (SLE749Q) /CSM/SL/SLE7-C/SLE749Q.Seq.d/                       1231   0.0
SLE190 (SLE190Q) /CSM/SL/SLE1-D/SLE190Q.Seq.d/                       1231   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U78759|U78759.1 Dictyostelium discoideum IfdA (ifdA) mRNA, partial cds.  1063   0.0    3
X80796|X80796.1 S.pombe eIF-4A mRNA for translation initiation factor.   115   4e-74  7
L40627|L40627.1 Schizosaccharomyces pombe cell cycle control protein eIF-4A (tif1) mRNA, complete cds.   115   5e-74  7
AL132828|AL132828.1 S.pombe chromosome I cosmid c1006.            115   4e-67  8
CF245450|CF245450.1 Fcylcold195 Fragilariopsis cylindrus SMART cDNA library (Clontech) Fragilariopsis cylindrus cDNA clone Antarctic 5', mRNA sequence.   113   6e-38  3
AU011976|AU011976.1 Schizosaccharomyces pombe cDNA, clone spc06170.    66   1e-32  5
AY098962|AY098962.1 Arabidopsis thaliana AT3g13920/MDC16_4 mRNA, complete cds.    94   1e-32  7
AU011946|AU011946.1 Schizosaccharomyces pombe cDNA, clone spc06139.    66   1e-32  5
AU009453|AU009453.1 Schizosaccharomyces pombe cDNA, clone spc04934.    66   1e-32  5
AY091304|AY091304.1 Arabidopsis thaliana putative eukaryotic initiation factor 4A (At3g13920) mRNA, complete cds.    94   1e-32  7
dna update 2003.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U78759_1(U78759|pid:none) Dictyostelium discoideum IfdA (ifdA) m...   620   0.0
CP001142_274(CP001142|pid:none) Phaeodactylum tricornutum CCAP 1...   422   e-116
(Q9CAI7) RecName: Full=Eukaryotic initiation factor 4A-3;       ...   418   e-115
(P41376) RecName: Full=Eukaryotic initiation factor 4A-1;       ...   413   e-114
(Q5KN60) RecName: Full=ATP-dependent RNA helicase eIF4A;        ...   412   e-113
AY081287_1(AY081287|pid:none) Arabidopsis thaliana eukaryotic tr...   412   e-113
(P41382) RecName: Full=Eukaryotic initiation factor 4A-10;      ...   412   e-113
AM460190_1(AM460190|pid:none) Vitis vinifera contig VV78X065511....   412   e-113
DQ440212_1(DQ440212|pid:none) Aedes aegypti clone AET-2104 initi...   410   e-113
AY086907_1(AY086907|pid:none) Arabidopsis thaliana clone 29310 m...   411   e-113
protein update 2009. 5.21
PSORT 

 psg: 0.75  gvh: 0.28  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for SFA112 is cyt
5' end seq. ID SFA112F
5' end seq. 
>SFA112F.Seq
ACTGTTGGCCTACTGGTTAAAATTTAAATCACAAACGAAAGAAATAAAACATGTCCGCAA
GTAATAAAGATGACCAACTCATGAAAAATGAGTTCGAAAGTACCTACGACAAAATTGTCG
ATTCATTCGACAATATGGAGCTCCCAAAGGATCTCTTAAGAGGTATCTACTCCTATGGTT
TTGAAAAGCCATCAGCCATCCAACAAAGAGCTATCATTCCACTCTCAAAAGGTTTTGATA
TTATTGCCCAAGCTCAATCTGGTACTGGTAAAACTGCTACCTTCACCGTTGGTATCTTAT
CTAGAATTGATATTTCAGTCCTCAAATGTCAAGCTCTCATCATTGCCCCAACTCGTGAAC
TTGCTCAACAAATTCAAAAGGTTGTTTTAGCTTTAGGTGAATATTTACACATTAAATCAT
ACGCATGTATTGGTGGTACTAAGATTACTGATGATATTCAAGCCCTCCAAAGTGGTGTAC
ACGTAGTTGTCGGTACCCCAGGTCGTGTCTTTGAAATGTTATCACGTGGTATCATTAATC
GNGACACCATTAGAATTTTCTGTCTTGATGAAGCTGATGAAATGTTAT----------
Length of 5' end seq. 588
3' end seq. ID SFA112Z
3' end seq. 
>SFA112Z.Seq
----------CGTCAAAAAGGATGAATTAACCCTTGAAGGTATTAGACAATTCTACGTCA
ACGTTGGTGTTGAACAAGGTAAATTTGAAGTTTTAACCGATCTCTACGAAACCCTCTCTA
TCACTCAATCCGTTATTTTCTGTAACACCCGTAGAAAGGTCGATTGGTTAACAAGCAAAA
TGACCGAACAAAAATTCACCGTCTCATCAACTCACGGTGACCAAAAGGATCGTGATGGTA
TCTTACAAGCTTTCCGTTCTGGTGCTACTCGTGTCCTCATCACCACCGATCTCTTAGCTC
GTGGTATTGATGTCCAACAAGTATCACTCGTTATCAACTTTGATTTACCAACCAACATTG
AAAATTACATTCATCGTATCGGTCGTTCAGGTCGTTTCGGTCGTAAGGGTGTTGCCATCA
ACTTTATCACTGACGCTGAAAGAGGTCAAATCGATGAATTATNCTCGTCATTACAATACC
TACATTGCCGAAATGCCACAAGACATTGGAAGTTTCCTCTAAACGAGTTCCAAAAATGGT
TTATAGTTCAGTTGGTCCCCATTTGGTGAGGGCCATAGGGTTTATAAATCGACCGCCCCC
CTCCCTATCCAATACTCACACCACAACAACATATTTCAACCGNCTNCATTCATAAAAACA
ACAAAAA
Length of 3' end seq. 657
Connected seq. ID SFA112P
Connected seq. 
>SFA112P.Seq
ACTGTTGGCCTACTGGTTAAAATTTAAATCACAAACGAAAGAAATAAAACATGTCCGCAA
GTAATAAAGATGACCAACTCATGAAAAATGAGTTCGAAAGTACCTACGACAAAATTGTCG
ATTCATTCGACAATATGGAGCTCCCAAAGGATCTCTTAAGAGGTATCTACTCCTATGGTT
TTGAAAAGCCATCAGCCATCCAACAAAGAGCTATCATTCCACTCTCAAAAGGTTTTGATA
TTATTGCCCAAGCTCAATCTGGTACTGGTAAAACTGCTACCTTCACCGTTGGTATCTTAT
CTAGAATTGATATTTCAGTCCTCAAATGTCAAGCTCTCATCATTGCCCCAACTCGTGAAC
TTGCTCAACAAATTCAAAAGGTTGTTTTAGCTTTAGGTGAATATTTACACATTAAATCAT
ACGCATGTATTGGTGGTACTAAGATTACTGATGATATTCAAGCCCTCCAAAGTGGTGTAC
ACGTAGTTGTCGGTACCCCAGGTCGTGTCTTTGAAATGTTATCACGTGGTATCATTAATC
GNGACACCATTAGAATTTTCTGTCTTGATGAAGCTGATGAAATGTTAT----------CG
TCAAAAAGGATGAATTAACCCTTGAAGGTATTAGACAATTCTACGTCAACGTTGGTGTTG
AACAAGGTAAATTTGAAGTTTTAACCGATCTCTACGAAACCCTCTCTATCACTCAATCCG
TTATTTTCTGTAACACCCGTAGAAAGGTCGATTGGTTAACAAGCAAAATGACCGAACAAA
AATTCACCGTCTCATCAACTCACGGTGACCAAAAGGATCGTGATGGTATCTTACAAGCTT
TCCGTTCTGGTGCTACTCGTGTCCTCATCACCACCGATCTCTTAGCTCGTGGTATTGATG
TCCAACAAGTATCACTCGTTATCAACTTTGATTTACCAACCAACATTGAAAATTACATTC
ATCGTATCGGTCGTTCAGGTCGTTTCGGTCGTAAGGGTGTTGCCATCAACTTTATCACTG
ACGCTGAAAGAGGTCAAATCGATGAATTATNCTCGTCATTACAATACCTACATTGCCGAA
ATGCCACAAGACATTGGAAGTTTCCTCTAAACGAGTTCCAAAAATGGTTTATAGTTCAGT
TGGTCCCCATTTGGTGAGGGCCATAGGGTTTATAAATCGACCGCCCCCCTCCCTATCCAA
TACTCACACCACAACAACATATTTCAACCGNCTNCATTCATAAAAACAACAAAAA
Length of connected seq. 1245
Full length Seq ID -
Full length Seq. -
Length of full length seq. -