>FCL-AD22 (FCL-AD22Q) /CSM/FCL/FCL-AD/FCL-AD22Q.Seq.d/
XXXXXXXXXXAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCT
GTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCC
AATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGT
GAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTC
TTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTT
AACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTT
GTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAA
GAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAA
GAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAA
AAAGAAGAAGTCAAAAAATCAACCAAAAAAGTTGATCATGACGAACTTTAAATGAAATAA
ATAAATAATAAAAAAAAAACAAATAAAAAAAAACTCGTGTTAAACTAA

---PAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGE
WVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFV
ANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKK
EEVKKSTKKVDHDEL*mk*innkkktnkkklvln*

Frame A:
---tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*
mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlc
cqprsrkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qk
rrsqkinqks*s*rtlneink**kknk*kktrvkl


Frame B:
---PAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGE
WVHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFV
ANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKK
EEVKKSTKKVDHDEL*mk*innkkktnkkklvln*


Frame C:
---qpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvn
gyth*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskklkislkrpll
ptkkpkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklmtkk
kksknqpkklimtnfk*nk*iikkkqikknsc*t

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        805   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        805   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        805   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        805   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        805   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        805   0.0
VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/                        805   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        805   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        805   0.0
VFD449 (VFD449Q) /CSM/VF/VFD4-C/VFD449Q.Seq.d/                        805   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   825  0.0  2
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  6e-12  2
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  4e-11  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  2e-09  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  2e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  2e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  2e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  2e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  2e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  2e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   238   1e-61
AF052978_1(AF052978|pid:none) Dirofilaria immitis calreticulin p...   149   5e-35
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   149   5e-35
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   148   1e-34
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   147   2e-34
JH0795(JH0795;B31409;F60977) calreticulin precursor - California...   147   3e-34
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   146   4e-34
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   146   6e-34
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   145   1e-33
AY510702_1(AY510702|pid:none) Haemonchus contortus calreticulin-...   145   1e-33

 psg: 0.75  gvh: 0.24  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.06  erl: 1.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: nuclear
	 20.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for FCL-AD22 is nuc

>FCL-AD22Z.Seq
----------AACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCT
GTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCC
AATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGT
GAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTC
TTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTT
AACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTT
GTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAA
GAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAA
GAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAA
AAAGAAGAAGTCAAAAAATCAACCAAAAAAGTTGATCATGACGAACTTTAAATGAAATAA
ATAAATAATAAAAAAAAAACAAATAAAAAAAAACTCGTGTTAAACTAA