>FCL-AC08 (FCL-AC08Q) /CSM/FCL/FCL-AC/FCL-AC08Q.Seq.d/
CGGACGCGTGGCGGACGCGTGGGATAAAAAGAATGTTTTAGGTGAAATTATTTATAAAGG
ACATCCAAGTTGGGCATTAATGTTAAATATTCAAACTGGTATTAGAAATGCGGTTGGTAA
ATCAATGGGTACAGAAGGAGGAGTTAATGCAAAGACACCACAACAGTATCATACATTAAG
GAAACCAAGTGAATTTAAGAGAAAGGAAGATTTTTATGTATCACCAAAAACACAAAGATT
CGAGAGTGCAGGCACTGCAATGACAAATGCTCACTCAACTGGTGCATTCAAATTTAAAGA
TTATTGTCCAAACGCTTTTAGATATCTACGTTATCTCTTTGGTATTGATACGGCGGATTT
CATGGTATCACTATGCAACACCCTCAAGAATGGCGAGAATGCACTACGTGAGCTACCAAC
ACCAGGTAAGAGTGGTAGTTTGTTCTTTTTCTCACACGATATGAAATTCATCATTAAAAC
CATTCCAAAGGATGAGGCTAAATTGTTACGTGATATTCTACCATCGTATTTGGAGCATAT
TCAGTCGAATCCAAATTCATTGTTACCTCGTTTCTTTGGTTTGTACAGAGTGAAACCACA
TTCAGGCAGACAGGTTXXXXXXXXXXTTCTTCTGTTTCTCCTGATGAATACTNTGAAAGA
TTCCAAGAATTTTTATCAACAATTATCGAATAATAATAATAAAAAAAAAAAAACTAAAAT
CCAAAAAAAAAAATCAAACCAAATAGTAATAATAATAATAATTTTAATAATATAATAATA
CTAATTGTCAATCATTACTTTTTTTTAAAAAATATATATAACATTACAAATAAATATTAA
AATAATTGTGTTTTTTTTAATTGAAAAAAAAAATTAAATAAATAAATAAAATAAAAAAAA
AAAAAAAAAAAAAAAAA

GRVADAWDKKNVLGEIIYKGHPSWALMLNIQTGIRNAVGKSMGTEGGVNAKTPQQYHTLR
KPSEFKRKEDFYVSPKTQRFESAGTAMTNAHSTGAFKFKDYCPNAFRYLRYLFGIDTADF
MVSLCNTLKNGENALRELPTPGKSGSLFFFSHDMKFIIKTIPKDEAKLLRDILPSYLEHI
QSNPNSLLPRFFGLYRVKPHSGRQV---

---LLFLLMNTXKDSKNFYQQLSNNNNKKKKTKIQKKKSNQIVIIIIILII**y*lsiit
ff*kiyitlqinikiivffliekkn*ink*nkkkkkkkk

Frame A:
rtrggrvg*kecfr*nyl*rtsklginvkysnwy*kcgw*ingyrrrs*ckdtttvsyik
etk*i*ekgrflcitkntkirecrhcndkcslnwciqi*rllskrf*istlslwy*yggf
hgitmqhpqewrectt*atntr*ew*fvlfltryeihh*nhskg*g*ivt*ystivfgay
sveskfivtsflwfvqsettfrqtg---

---ffcfs**il*kiprifinnyriiiikkkklkskkknqtk******f**ynntncqsl
lffkkyi*hyk*ilk*lcff*lkkkik*inkikkkkkkkk

Frame B:
GRVADAWDKKNVLGEIIYKGHPSWALMLNIQTGIRNAVGKSMGTEGGVNAKTPQQYHTLR
KPSEFKRKEDFYVSPKTQRFESAGTAMTNAHSTGAFKFKDYCPNAFRYLRYLFGIDTADF
MVSLCNTLKNGENALRELPTPGKSGSLFFFSHDMKFIIKTIPKDEAKLLRDILPSYLEHI
QSNPNSLLPRFFGLYRVKPHSGRQV---

---ssvspdeyxerfqeflstiie****kkkn*npkkkikpnsnnnnnfnniiilivnhy
fflkniynitnky*nncvffn*kkklnk*ik*kkkkkkk

Frame C:
dawrtrgikrmf*vklfikdiqvgh*c*ifklvlemrlvnqwvqkeelmqrhhnsiih*g
nqvnlrerkifmyhqkhkdsrvqalq*qmltqlvhsnlkiivqtlldiyvislvlirris
wyhyatpsrmarmhyvsyqhqvrvvvcsfshti*nsslkpfqrmrlncyvifyhriwsif
sriqihcylvslvcte*nhiqadr---

---LLFLLMNTXKDSKNFYQQLSNNNNKKKKTKIQKKKSNQIVIIIIILII**y*lsiit
ff*kiyitlqinikiivffliekkn*ink*nkkkkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FCL-AC08 (FCL-AC08Q) /CSM/FCL/FCL-AC/FCL-AC08Q.Seq.d/                1322   0.0
SFA226 (SFA226Q) /CSM/SF/SFA2-B/SFA226Q.Seq.d/                       1090   0.0
SLK612 (SLK612Q) /CSM/SL/SLK6-A/SLK612Q.Seq.d/                        809   0.0
VSE576 (VSE576Q) /CSM/VS/VSE5-D/VSE576Q.Seq.d/                        113   5e-24
CHI439 (CHI439Q) /CSM/CH/CHI4-B/CHI439Q.Seq.d/                         88   3e-16
VSK269 (VSK269Q) /CSM/VS/VSK2-C/VSK269Q.Seq.d/                         40   0.058
AHL408 (AHL408Q) /CSM/AH/AHL4-A/AHL408Q.Seq.d/                         40   0.058
VSJ311 (VSJ311Q) /CSM/VS/VSJ3-A/VSJ311Q.Seq.d/                         38   0.23
SSL151 (SSL151Q) /CSM/SS/SSL1-C/SSL151Q.Seq.d/                         38   0.23
SSK895 (SSK895Q) /CSM/SS/SSK8-D/SSK895Q.Seq.d/                         38   0.23

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI248494|BI248494.1 602992909F1 NCI_CGAP_Mam5 Mus musculus cDNA clone IMAGE:5148579 5', mRNA sequence.    56  8e-04  1
CD650187|CD650187.1 CvGil00892 Crassostrea virginica Gill Crassostrea virginica cDNA 5', mRNA sequence.    52  0.012  1
BG974025|BG974025.1 602843710F1 NIH_CGAP_Mam4 Mus musculus cDNA clone IMAGE:4979283 5', mRNA sequence.    50  0.048  1
AY254474|AY254474.1 Dictyostelium discoideum frizzled protein (frzA) gene, complete cds.    46  0.077  2
AL393425|AL393425.1 T3 end of clone AR0AA010H09 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    40  0.093  2
BU908867|BU908867.1 AGENCOURT_10487926 NICHD XGC Emb1 Xenopus laevis cDNA clone IMAGE:6634425 5', mRNA sequence.    48  0.19  1
CD203600|CD203600.1 Ls_AM1_11A10_T7 Litomosoides sigmodontis adult male 1 Litomosoides sigmodontis cDNA clone Ls_AM1_11A10 5' similar to Q8HN46 (Q8HN46) Cytochrome c oxidase subunit II, mRNA sequence.    48  0.19  1
BI658825|BI658825.1 603301975F1 NIH_CGAP_Mam4 Mus musculus cDNA clone IMAGE:5347602 5', mRNA sequence.    48  0.19  1
BQ593755|BQ593755.1 E012763-024-026-I13-SP6 MPIZ-ADIS-024-developing root Beta vulgaris cDNA clone 024-026-I13 5-PRIME, mRNA sequence.    48  0.19  1
AX346270|AX346270.1 Sequence 1341 from Patent WO0200928.          38  0.21  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FJ024081_1(FJ024081|pid:none) Physcomitrella patens phosphatidyl...   165   2e-39
AC091775_10(AC091775|pid:none) Oryza sativa chromosome 3 BAC OSJ...   158   2e-37
(Q56YP2) RecName: Full=Phosphatidylinositol-4-phosphate 5-kinase...   144   4e-33
AF019380_1(AF019380|pid:none) Arabidopsis thaliana putative phos...   144   4e-33
AB005902_1(AB005902|pid:none) Arabidopsis thaliana mRNA for AtPI...   141   3e-32
AF157047_1(AF157047|pid:none) Nicotiana rustica putative phospha...   140   4e-32
AK120829_1(AK120829|pid:none) Oryza sativa Japonica Group cDNA c...   140   6e-32
AM461285_8(AM461285|pid:none) Vitis vinifera contig VV79X002621....   139   2e-31
(O48709) RecName: Full=Phosphatidylinositol-4-phosphate 5-kinase...   138   2e-31
CR954207_399(CR954207|pid:none) Ostreococcus tauri strain OTTH05...   138   3e-31

 psg: 0.74  gvh: 0.38  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.02
 nuc: 0.07  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 40.0 %: cytoplasmic
	 16.0 %: cytoskeletal

>> prediction for FCL-AC08 is nuc

>FCL-AC08F.Seq
CGGACGCGTGGCGGACGCGTGGGATAAAAAGAATGTTTTAGGTGAAATTATTTATAAAGG
ACATCCAAGTTGGGCATTAATGTTAAATATTCAAACTGGTATTAGAAATGCGGTTGGTAA
ATCAATGGGTACAGAAGGAGGAGTTAATGCAAAGACACCACAACAGTATCATACATTAAG
GAAACCAAGTGAATTTAAGAGAAAGGAAGATTTTTATGTATCACCAAAAACACAAAGATT
CGAGAGTGCAGGCACTGCAATGACAAATGCTCACTCAACTGGTGCATTCAAATTTAAAGA
TTATTGTCCAAACGCTTTTAGATATCTACGTTATCTCTTTGGTATTGATACGGCGGATTT
CATGGTATCACTATGCAACACCCTCAAGAATGGCGAGAATGCACTACGTGAGCTACCAAC
ACCAGGTAAGAGTGGTAGTTTGTTCTTTTTCTCACACGATATGAAATTCATCATTAAAAC
CATTCCAAAGGATGAGGCTAAATTGTTACGTGATATTCTACCATCGTATTTGGAGCATAT
TCAGTCGAATCCAAATTCATTGTTACCTCGTTTCTTTGGTTTGTACAGAGTGAAACCACA
TTCAGGCAGACAGGTT----------

>FCL-AC08Z.Seq
----------TTCTTCTGTTTCTCCTGATGAATACTNTGAAAGATTCCAAGAATTTTTAT
CAACAATTATCGAATAATAATAATAAAAAAAAAAAAACTAAAATCCAAAAAAAAAAATCA
AACCAAATAGTAATAATAATAATAATTTTAATAATATAATAATACTAATTGTCAATCATT
ACTTTTTTTTAAAAAATATATATAACATTACAAATAAATATTAAAATAATTGTGTTTTTT
TTAATTGAAAAAAAAAATTAAATAAATAAATAAAATAAAAAAAAAAAAAAAAAAAAAAAA
A

>FCL-AC08P.Seq
CGGACGCGTGGCGGACGCGTGGGATAAAAAGAATGTTTTAGGTGAAATTATTTATAAAGG
ACATCCAAGTTGGGCATTAATGTTAAATATTCAAACTGGTATTAGAAATGCGGTTGGTAA
ATCAATGGGTACAGAAGGAGGAGTTAATGCAAAGACACCACAACAGTATCATACATTAAG
GAAACCAAGTGAATTTAAGAGAAAGGAAGATTTTTATGTATCACCAAAAACACAAAGATT
CGAGAGTGCAGGCACTGCAATGACAAATGCTCACTCAACTGGTGCATTCAAATTTAAAGA
TTATTGTCCAAACGCTTTTAGATATCTACGTTATCTCTTTGGTATTGATACGGCGGATTT
CATGGTATCACTATGCAACACCCTCAAGAATGGCGAGAATGCACTACGTGAGCTACCAAC
ACCAGGTAAGAGTGGTAGTTTGTTCTTTTTCTCACACGATATGAAATTCATCATTAAAAC
CATTCCAAAGGATGAGGCTAAATTGTTACGTGATATTCTACCATCGTATTTGGAGCATAT
TCAGTCGAATCCAAATTCATTGTTACCTCGTTTCTTTGGTTTGTACAGAGTGAAACCACA
TTCAGGCAGACAGGTT----------TTCTTCTGTTTCTCCTGATGAATACTNTGAAAGA
TTCCAAGAATTTTTATCAACAATTATCGAATAATAATAATAAAAAAAAAAAAACTAAAAT
CCAAAAAAAAAAATCAAACCAAATAGTAATAATAATAATAATTTTAATAATATAATAATA
CTAATTGTCAATCATTACTTTTTTTTAAAAAATATATATAACATTACAAATAAATATTAA
AATAATTGTGTTTTTTTTAATTGAAAAAAAAAATTAAATAAATAAATAAAATAAAAAAAA
AAAAAAAAAAAAAAAAA