>FCL-AB19 (FCL-AB19Q) /CSM/FCL/FCL-AB/FCL-AB19Q.Seq.d/
XXXXXXXXXXACCACCACATGCTGCCGCTGCTATCAAAGAGCTATCGATGCTGAAATGGA
ATTAGTAGTTTGTATCACTGAAGGTATCCCACAACAAGATATGGTTAAAGTTAAATATTT
ACTTAATAAACAAAATAAAACTCGTTTAATTGGACCAAATTGTCCAGGTATTATTAAACC
AGGTGAATGCAAGATTGGTATTATGCCAGGTCACATCCATAAACCAGGTAAGATTGGTAT
CGTTTCACGTTCAGGTACATTAACTTATGAAGCAGTCGCTCAAACTACCGCTGTTGGTTT
AGGTCAATCAACTTGTATTGGTATTGGTGGTGATCCATTCAATGGAACAAACTTTATCGA
TTGTTTAAAGATGTTCACTCAAGATCCACAAACCGAAGGTATCATTTTAATTGGTGAAAT
CGGTGGTGAAGCCGAAGAAGAAGCCGCCCAATGGTTAATTGATAATCCAACCGATAAACC
AGTCGTCTCTTTCATCGCTGGTCTCTCTGCACCACCAGGTAGAAGAATGGGTCATGCTGG
TGCCATCATCAGTGGTGGTAAAGGTGATGCCAACAGTAAAATTGAAGCTTTGAAAGCTGC
AGGTGTTACAGTTACTTTTTCACCAGCCAAATTAGGTGAAACTATACTACAAAAAATGAA
TGAAAAAAAAATAAATAAACTAATCAATTTTTCAATTTTTCAATAAT

---TTCCRCYQRAIDAEMELVVCITEGIPQQDMVKVKYLLNKQNKTRLIGPNCPGIIKPG
ECKIGIMPGHIHKPGKIGIVSRSGTLTYEAVAQTTAVGLGQSTCIGIGGDPFNGTNFIDC
LKMFTQDPQTEGIILIGEIGGEAEEEAAQWLIDNPTDKPVVSFIAGLSAPPGRRMGHAGA
IISGGKGDANSKIEALKAAGVTVTFSPAKLGETILQKMNEKKINKLINFSIFQ*

Frame A:
---pphaaaaikelsmlkwn**fvslkvshnkiwlklniylinkiklv*ldqivqvllnq
vnarlvlcqvtsinqvrlvsfhvqvh*lmkqslklpllv*vnqlvlvlvvihsmeqtlsi
v*rcslkihkpkvsf*lvksvvkpkkkppng*liiqpinqsslsslvslhhqveewvmlv
pssvvvkvmptvklkl*klqvlqllfhqpn*vklyykk*mkkk*in*sifqffnn


Frame B:
---hhmlpllsksyrc*ngisslyh*rypttryg*s*ift**tk*nsfnwtklsryy*tr
*mqdwyyarshp*tr*dwyrftfryinl*ssrsnyrcwfrsinlywyww*siqwnklyrl
fkdvhsrstnrryhfnw*nrw*srrrsrpmvn**snr*tsrlfhrwslcttr*kngscwc
hhqww*r*cqq*n*sfescrcysyfftsqir*nyttkne*kknk*tnqffnfsi


Frame C:
---TTCCRCYQRAIDAEMELVVCITEGIPQQDMVKVKYLLNKQNKTRLIGPNCPGIIKPG
ECKIGIMPGHIHKPGKIGIVSRSGTLTYEAVAQTTAVGLGQSTCIGIGGDPFNGTNFIDC
LKMFTQDPQTEGIILIGEIGGEAEEEAAQWLIDNPTDKPVVSFIAGLSAPPGRRMGHAGA
IISGGKGDANSKIEALKAAGVTVTFSPAKLGETILQKMNEKKINKLINFSIFQ*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FCL-AB19 (FCL-AB19Q) /CSM/FCL/FCL-AB/FCL-AB19Q.Seq.d/                1328   0.0
VFI484 (VFI484Q) /CSM/VF/VFI4-D/VFI484Q.Seq.d/                       1294   0.0
VFC865 (VFC865Q) /CSM/VF/VFC8-C/VFC865Q.Seq.d/                       1259   0.0
SSH760 (SSH760Q) /CSM/SS/SSH7-C/SSH760Q.Seq.d/                         38   0.18
VSE317 (VSE317Q) /CSM/VS/VSE3-A/VSE317Q.Seq.d/                         36   0.70
VSB628 (VSB628Q) /CSM/VS/VSB6-B/VSB628Q.Seq.d/                         36   0.70
AHB748 (AHB748Q) /CSM/AH/AHB7-B/AHB748Q.Seq.d/                         36   0.70
VSD515 (VSD515Q) /CSM/VS/VSD5-A/VSD515Q.Seq.d/                         34   2.8
VSA633 (VSA633Q) /CSM/VS/VSA6-B/VSA633Q.Seq.d/                         34   2.8
VSA561 (VSA561Q) /CSM/VS/VSA5-C/VSA561Q.Seq.d/                         34   2.8

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U23408|U23408.1 Dictyostelium discoideum succinyl coenzyme A synthetase alpha subunit (SCS-alpha) mRNA, complete cds.  1215  0.0  3
AL436490|AL436490.1 T3 end of clone BC0AA003F04 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    74  1e-44  7
AX621830|AX621830.1 Sequence 4793 from Patent WO02094868.         50  2e-30  7
AY238445|AY238445.1 Neocallimastix frontalis succinyl-CoA synthetase alpha subunit mRNA, complete cds.    70  5e-27  5
AF493054|AF493054.1 Neocallimastix patriciarum succinyl-CoA synthetase, alpha subunit (scsA) mRNA, complete cds.    70  7e-27  5
AW443049|AW443049.1 EST307979 tomato mixed elicitor, BTI Lycopersicon esculentum cDNA clone cLET42N2 5', mRNA sequence.    64  2e-24  4
BI422741|BI422741.1 EST533407 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC71B23 5' end, mRNA sequence.    64  4e-24  4
AY167586|AY167586.1 Lycopersicon esculentum succinyl-CoA ligase alpha subunit (SCOA) mRNA, complete cds; nuclear gene for mitochondrial product.    64  1e-23  4
AI487985|AI487985.1 EST246307 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED19H22, mRNA sequence.    64  2e-23  4
BI511131|BI511131.1 BB160004B10H12 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160004B10H12 5', mRNA sequence.    66  7e-23  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36967) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   385   e-106
S65966(S65966;S65891) succinate-CoA ligase (GDP-forming) (EC 6.2...   373   e-103
EU959743_1(EU959743|pid:none) Zea mays clone 219746 succinyl-CoA...   296   3e-79
CR954203_584(CR954203|pid:none) Ostreococcus tauri strain OTTH05...   293   3e-78
DQ235193_1(DQ235193|pid:none) Solanum tuberosum clone 167F09 suc...   292   7e-78
S30579(S30579) succinate-CoA ligase (GDP-forming) (EC 6.2.1.4) a...   291   1e-77
AY087899_1(AY087899|pid:none) Arabidopsis thaliana clone 39462 m...   290   2e-77
(P68209) RecName: Full=Succinyl-CoA ligase [GDP-forming] subunit...   290   2e-77
CP000583_582(CP000583|pid:none) Ostreococcus lucimarinus CCE9901...   290   3e-77
BC081520_1(BC081520|pid:none) Danio rerio succinate-CoA ligase, ...   290   4e-77

 psg: 0.62  gvh: 0.38  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for FCL-AB19 is cyt

>FCL-AB19Z.Seq
----------ACCACCACATGCTGCCGCTGCTATCAAAGAGCTATCGATGCTGAAATGGA
ATTAGTAGTTTGTATCACTGAAGGTATCCCACAACAAGATATGGTTAAAGTTAAATATTT
ACTTAATAAACAAAATAAAACTCGTTTAATTGGACCAAATTGTCCAGGTATTATTAAACC
AGGTGAATGCAAGATTGGTATTATGCCAGGTCACATCCATAAACCAGGTAAGATTGGTAT
CGTTTCACGTTCAGGTACATTAACTTATGAAGCAGTCGCTCAAACTACCGCTGTTGGTTT
AGGTCAATCAACTTGTATTGGTATTGGTGGTGATCCATTCAATGGAACAAACTTTATCGA
TTGTTTAAAGATGTTCACTCAAGATCCACAAACCGAAGGTATCATTTTAATTGGTGAAAT
CGGTGGTGAAGCCGAAGAAGAAGCCGCCCAATGGTTAATTGATAATCCAACCGATAAACC
AGTCGTCTCTTTCATCGCTGGTCTCTCTGCACCACCAGGTAGAAGAATGGGTCATGCTGG
TGCCATCATCAGTGGTGGTAAAGGTGATGCCAACAGTAAAATTGAAGCTTTGAAAGCTGC
AGGTGTTACAGTTACTTTTTCACCAGCCAAATTAGGTGAAACTATACTACAAAAAATGAA
TGAAAAAAAAATAAATAAACTAATCAATTTTTCAATTTTTCAATAAT