>FCL-AB13 (FCL-AB13Q) /CSM/FCL/FCL-AB/FCL-AB13Q.Seq.d/
XXXXXXXXXXAACCCAACTTGATCATGGTGTTTTAGTTGTTGGTTATGGTTCAGGTTCAA
GTTCATCATCTGGTTCATCATCTGGTAAATCATCATCATCATCATCAACTGGTGGTAAAA
CTTCATCCTCATCATCATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTTCATCAT
CATCATCATCAGGTAAAACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAATCAGGTT
CCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCTCTGCTT
CTGGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTT
CAGGCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACTCATGGG
GTACTTCATGGGGTATGGATGGTTACATTTTTATGAGCAAAGATAGAAATAACAATTGTG
GTATCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTTTTAATT
GTCCGACTAACATTAATTTGATTTGTAATAAATAAAAAAAATAAAAATAAAAAATAAAAT
AAAAAAAAAAAAACTATTTTTCATTTTAAAAAAAAAAAAAAAAAAAAAAA

---TQLDHGVLVVGYGSGSSSSSGSSSGKSSSSSSTGGKTSSSSSSGKASSSSSGKASSS
SSSGKTSSAASSTSGSQSGSQSGSQSGQSTGSQSGQTSASGQASASGSGSGSGSGSGSGS
GSGAVEASSGNYWIVKNSWGTSWGMDGYIFMSKDRNNNCGIATMASFPTASSN*nfifnc
ptninlicnk*kk*k*kik*kkknyfsf*kkkkkkk

Frame A:
---TQLDHGVLVVGYGSGSSSSSGSSSGKSSSSSSTGGKTSSSSSSGKASSSSSGKASSS
SSSGKTSSAASSTSGSQSGSQSGSQSGQSTGSQSGQTSASGQASASGSGSGSGSGSGSGS
GSGAVEASSGNYWIVKNSWGTSWGMDGYIFMSKDRNNNCGIATMASFPTASSN*nfifnc
ptninlicnk*kk*k*kik*kkknyfsf*kkkkkkk


Frame B:
---pnlimvf*llvmvqvqvhhlvhhlvnhhhhhqlvvklhphhhqvklhhhhqaklhhh
hhqvklhlllhqplvlnqvpnqvanqanppvhnqvkplllvkhqhqvlvlaqvqvqvqvq
aqvllrphlvttgslkthgvlhgvwmvtfl*akieitivvsqqwllsqlphqikilfliv
rltli*fvinkknknkk*nkkkktifhfkkkkkkk


Frame C:
---pt*swcfscwlwfrfkfiiwfiiw*iiiiiinww*nfiliiir*sfiiiirqsfiii
iir*nficcfinlwfsirfpir*pirpihrftirsnlcfwssisirfwfwlrfrfrfrfr
lrcc*gliw*lldr*klmgyfmgygwlhfyeqr*k*qlwyrnngffpncliklkfyf*ls
d*h*fdl**ikkikiknkikkkklffilkkkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO439 (VFO439Q) /CSM/VF/VFO4-B/VFO439Q.Seq.d/                        392   e-108
VFO416 (VFO416Q) /CSM/VF/VFO4-A/VFO416Q.Seq.d/                        392   e-108
SLG572 (SLG572Q) /CSM/SL/SLG5-C/SLG572Q.Seq.d/                        392   e-108
SLC532 (SLC532Q) /CSM/SL/SLC5-B/SLC532Q.Seq.d/                        392   e-108
FCL-AB13 (FCL-AB13Q) /CSM/FCL/FCL-AB/FCL-AB13Q.Seq.d/                 392   e-108
FC-AN18 (FC-AN18Q) /CSM/FC/FC-AN/FC-AN18Q.Seq.d/                      392   e-108
VFE627 (VFE627Q) /CSM/VF/VFE6-B/VFE627Q.Seq.d/                        387   e-106
SSM682 (SSM682Q) /CSM/SS/SSM6-D/SSM682Q.Seq.d/                        385   e-106
VFO828 (VFO828Q) /CSM/VF/VFO8-B/VFO828Q.Seq.d/                        377   e-103
VFN392 (VFN392Q) /CSM/VF/VFN3-D/VFN392Q.Seq.d/                        377   e-103

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L36204|L36204.1 Dictyostelium discoideum cysteine proteinase (CP4) mRNA, complete cds.   315  0.0  4
L36205|L36205.1 Dictyostelium discoideum cysteine proteinase CP5 mRNA, complete cds.   100  1e-30  2
U72746|U72746.1 Dictyostelium discoideum cysteine proteinase (cprG) mRNA, complete cds.   117  6e-28  3
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    92  8e-26  3
X03344|X03344.1 Dictyostelium discoideum mRNA for cysteine proteinase 2.    76  1e-20  2
M16039|M16039.1 Dictyostelium discoideum pst-cath gene encoding pst-cathepsin, complete cds.    64  1e-16  2
U72745|U72745.1 Dictyostelium discoideum cysteine proteinase (cprF) mRNA, complete cds.    60  6e-13  3
BJ171907|BJ171907.1 Physcomitrella patens subsp. patens cDNA clone:pph30i20, 3' end, single read.    60  3e-05  1
AI067610|AI067610.1 EST209292 Schistosoma mansoni, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMNCC16 5' end similar to cathepsin L, mRNA sequence.    42  3e-04  2
AI975022|AI975022.1 EST269616 Schistosoma mansoni female, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMFAA90 5' end similar to cathepsin L, mRNA sequence.    42  4e-04  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L36204_1(L36204|pid:none) Dictyostelium discoideum cysteine prot...   110   3e-23
(Q94504) RecName: Full=Cysteine proteinase 7;          EC=3.4.22...    81   3e-14
(P04989) RecName: Full=Cysteine proteinase 2;          EC=3.4.22...    79   1e-13
(P54640) RecName: Full=Cysteine proteinase 5;          EC=3.4.22...    77   5e-13
L36205_1(L36205|pid:none) Dictyostelium discoideum cysteine prot...    77   5e-13
DQ280314_1(DQ280314|pid:none) Hymeniacidon perlevis cathepsin L ...    77   5e-13
AX497240_1(AX497240|pid:none) Sequence 7 from Patent WO0229058.        76   7e-13
(Q94503) RecName: Full=Cysteine proteinase 6;          EC=3.4.22...    76   7e-13
BC051665_1(BC051665|pid:none) Mus musculus cDNA sequence BC05166...    74   3e-12
AK145559_1(AK145559|pid:none) Mus musculus blastocyst blastocyst...    74   3e-12

 psg: 0.75  gvh: 0.57  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.46  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for FCL-AB13 is mit

>FCL-AB13Z.Seq
----------AACCCAACTTGATCATGGTGTTTTAGTTGTTGGTTATGGTTCAGGTTCAA
GTTCATCATCTGGTTCATCATCTGGTAAATCATCATCATCATCATCAACTGGTGGTAAAA
CTTCATCCTCATCATCATCAGGTAAAGCTTCATCATCATCATCAGGCAAAGCTTCATCAT
CATCATCATCAGGTAAAACTTCATCTGCTGCTTCATCAACCTCTGGTTCTCAATCAGGTT
CCCAATCAGGTAGCCAATCAGGCCAATCCACCGGTTCACAATCAGGTCAAACCTCTGCTT
CTGGTCAAGCATCAGCATCAGGTTCTGGTTCTGGCTCAGGTTCAGGTTCAGGTTCAGGTT
CAGGCTCAGGTGCTGTTGAGGCCTCATCTGGTAACTACTGGATCGTTAAAAACTCATGGG
GTACTTCATGGGGTATGGATGGTTACATTTTTATGAGCAAAGATAGAAATAACAATTGTG
GTATCGCAACAATGGCTTCTTTCCCAACTGCCTCATCAAATTAAAATTTTATTTTTAATT
GTCCGACTAACATTAATTTGATTTGTAATAAATAAAAAAAATAAAAATAAAAAATAAAAT
AAAAAAAAAAAAACTATTTTTCATTTTAAAAAAAAAAAAAAAAAAAAAAA