>FCL-AA01 (FCL-AA01Q) /CSM/FCL/FCL-AA/FCL-AA01Q.Seq.d/
GCAAATAATAATATTATGGGTATTGACTTTGGTACACATTTCGCATGTGTTGGTATTTTC
AAGAATGAAAGAATTGAAATCTGTCCAAATCAACAAGGTAACAGAACTACTCCATCAGTT
GTTTCATTCGTTGGTGACGATAAATTAGTTGGTGATGAAGCTAAAGCACAAATGGATAGA
AATCCATTAAATACAATTTATGATGTTAAAAGATTATTAGGTAGAAAAACTACTGACGAA
TTATTTGATCATGAAGTTAAAAAATTATCATTTAAAGTTACAACATATGAAGATAATAAT
GAAAAGATTGAATTTCAAGTTAACTATAAATCAAATGTTGTTACATTAACACCAATTGAA
ATTGCAACCAGTATTTTAGAACAAATTAAACATACTGCAGAGACATTTATTGGTGGTGAA
TCAATTAAGAAGGCCGTTATATCAGTACCAACAGATTTCACAGAGAAACAAAGAAACGAT
TTGAAAGAGGCAGCCACAGCAGCAGGTATTACAGTTGTTAGAATGATTCATGAACATTCA
GCAGTTGCATTAGCATATGGTTATGATCAAGTTAAGGAATGTTCAGAGACAACTAATGAA
TCAXXXXXXXXXXTTCCTTCATCTTCCCTCATTCCATGCAAACGTACTTTCACTTTAAAT
ACTTCCTCTGAAAATCAAGATAATCTTAATATGGCAGTCTATCAAGGTAGTGCACCACTC
GCCAAAGATAATCAATTGGTTTCACGTTTCATCTTTAAATCTTCTCCAAATCCAATCGAT
GTCACTTTTGAAATTGATAATAACAATACTTTATTAATTAAATCCGAACAAGCAAATTAT
TCAATTAAATTTTAAAAATTAAACTATTAAATATTATAAAAAAATTCAAATAATAATAAT
AATAATAATAATTAATAACAAATAAATAAAAATAAAAATAAAAAAAAAATAAAAAAAAGT
AATACACGGTAAATAATTTGATTTTCAAATTAAAAAATAGTATTAAAAAAAAAAAAAAAA
ATTA

ANNNIMGIDFGTHFACVGIFKNERIEICPNQQGNRTTPSVVSFVGDDKLVGDEAKAQMDR
NPLNTIYDVKRLLGRKTTDELFDHEVKKLSFKVTTYEDNNEKIEFQVNYKSNVVTLTPIE
IATSILEQIKHTAETFIGGESIKKAVISVPTDFTEKQRNDLKEAATAAGITVVRMIHEHS
AVALAYGYDQVKECSETTNES---

---PSSSLIPCKRTFTLNTSSENQDNLNMAVYQGSAPLAKDNQLVSRFIFKSSPNPIDVT
FEIDNNNTLLIKSEQANYSIKF*klny*il*knsnnnnnnnn**qinknknkkkikksnt
r*ii*fsn*kivlkkkkki

Frame A:
ANNNIMGIDFGTHFACVGIFKNERIEICPNQQGNRTTPSVVSFVGDDKLVGDEAKAQMDR
NPLNTIYDVKRLLGRKTTDELFDHEVKKLSFKVTTYEDNNEKIEFQVNYKSNVVTLTPIE
IATSILEQIKHTAETFIGGESIKKAVISVPTDFTEKQRNDLKEAATAAGITVVRMIHEHS
AVALAYGYDQVKECSETTNES---

---flhlpsfhanvlsl*ilplkikiiliwqsikvvhhspkiinwfhvsslnllqiqsms
llkliitily*lnpnkqiiqlnfkn*tikyykkiqiiiiiiiinnk*ikikikkk*kkvi
hgk*fdfqikk*y*kkkkkl

Frame B:
qiiilwvltlvhishvlvfsrmkelksvqinkvtellhqlfhslvtin*lvmklkhkwie
ih*iqfmmlkdy*veklltnylimklknyhlklqhmkiimkrlnfkltinqmllh*hqlk
lqpvf*nklnilqrhllvvnqlrrplyqyqqisqrnketi*krqpqqqvlqlle*fmniq
qlh*hmvmiklrnvqrqlmn---

---sfifphsmqtyfhfkyfl*ksr*s*ygslsr*cttrqr*sigftfhl*ifsksnrch
f*n***qyfin*irtsklfn*ilkikllniikkfk*******litnk*k*k*kknkkk*y
tvnnlifklknsikkkkkn

Frame C:
k**yygy*lwytfrmcwyfqe*kn*nlskstr*qnysiscfirw*r*isw**s*stng*k
sikynl*c*kiir*kny*rii*s*s*kiii*syni*r***kd*iss*l*ikccyintn*n
cnqyfrtn*tycrdiyww*in*egryistnrfhretkkrfergshssryysc*nds*tfs
scisiwl*ss*gmfrdn**i---

---PSSSLIPCKRTFTLNTSSENQDNLNMAVYQGSAPLAKDNQLVSRFIFKSSPNPIDVT
FEIDNNNTLLIKSEQANYSIKF*klny*il*knsnnnnnnnn**qinknknkkkikksnt
r*ii*fsn*kivlkkkkki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FCL-AA01 (FCL-AA01Q) /CSM/FCL/FCL-AA/FCL-AA01Q.Seq.d/                1592   0.0
FC-IC0721 (FC-IC0721Q) /CSM/FC-IC/FC-IC0721Q.Seq.d/                   819   0.0
SHE286 (SHE286Q) /CSM/SH/SHE2-D/SHE286Q.Seq.d/                         40   0.065
SFK215 (SFK215Q) /CSM/SF/SFK2-A/SFK215Q.Seq.d/                         40   0.065
SFK163 (SFK163Q) /CSM/SF/SFK1-C/SFK163Q.Seq.d/                         40   0.065
CHQ519 (CHQ519Q) /CSM/CH/CHQ5-A/CHQ519Q.Seq.d/                         40   0.065
CHG524 (CHG524Q) /CSM/CH/CHG5-A/CHG524Q.Seq.d/                         40   0.065
CFI746 (CFI746Q) /CSM/CF/CFI7-B/CFI746Q.Seq.d/                         40   0.065
AHO630 (AHO630Q) /CSM/AH/AHO6-B/AHO630Q.Seq.d/                         40   0.065
AFL219 (AFL219Q) /CSM/AF/AFL2-A/AFL219Q.Seq.d/                         40   0.065

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    32  0.12  11
AL435810|AL435810.1 T3 end of clone XBB0AA001E10 of library XBB0AA from strain CBS 4732  of Pichia angusta.    46  0.12  2
AC137365|AC137365.1 Rattus norvegicus clone CH230-unknown, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44  0.13  4
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    40  0.14  11
AC096328|AC096328.9 Rattus norvegicus clone CH230-78B21, WORKING DRAFT SEQUENCE, 2 unordered pieces.    44  0.16  3
AC068637|AC068637.12 Homo sapiens chromosome 3 clone RP11-440C18, WORKING DRAFT SEQUENCE, 1 unordered pieces.    36  0.35  6
U20432|U20432.1 Dictyostelium discoideum TagB (tagB) gene, complete cds.    40  0.62  2
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32  0.80  12
U49932|U49932.1 Pichia angusta heat shock protein 70 homolog (HSA2) gene, complete cds.    46  0.84  1
AL419034|AL419034.1 T7 end of clone AX0AA036E02 of library AX0AA from strain CBS 7064 of Pichia farinosa.    46  0.84  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54MR6) RecName: Full=Heat shock 70-related protein 5;               401   e-110
(P14834) RecName: Full=Heat shock 70 kDa protein; Flags: Fragmen...   174   3e-42
M26595_1(M26595|pid:none) T.cruzi heat shock protein (HSP70) gen...   174   4e-42
AM502246_293(AM502246|pid:none) Leishmania infantum chromosome 28.    174   5e-42
AM502246_292(AM502246|pid:none) Leishmania infantum chromosome 28.    174   5e-42
CT005266_287(CT005266|pid:none) Leishmania major strain Friedlin...   174   5e-42
AY423868_1(AY423868|pid:none) Leishmania tarentolae 70 kDa heat ...   173   9e-42
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   172   1e-41
EF442767_1(EF442767|pid:none) Trypanosoma rangeli isolate H14 cl...   172   1e-41
FJ222459_1(FJ222459|pid:none) Trypanosoma cruzi heat shock prote...   172   1e-41

 psg: 0.75  gvh: 0.28  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for FCL-AA01 is cyt

>FCL-AA01F.Seq
GCAAATAATAATATTATGGGTATTGACTTTGGTACACATTTCGCATGTGTTGGTATTTTC
AAGAATGAAAGAATTGAAATCTGTCCAAATCAACAAGGTAACAGAACTACTCCATCAGTT
GTTTCATTCGTTGGTGACGATAAATTAGTTGGTGATGAAGCTAAAGCACAAATGGATAGA
AATCCATTAAATACAATTTATGATGTTAAAAGATTATTAGGTAGAAAAACTACTGACGAA
TTATTTGATCATGAAGTTAAAAAATTATCATTTAAAGTTACAACATATGAAGATAATAAT
GAAAAGATTGAATTTCAAGTTAACTATAAATCAAATGTTGTTACATTAACACCAATTGAA
ATTGCAACCAGTATTTTAGAACAAATTAAACATACTGCAGAGACATTTATTGGTGGTGAA
TCAATTAAGAAGGCCGTTATATCAGTACCAACAGATTTCACAGAGAAACAAAGAAACGAT
TTGAAAGAGGCAGCCACAGCAGCAGGTATTACAGTTGTTAGAATGATTCATGAACATTCA
GCAGTTGCATTAGCATATGGTTATGATCAAGTTAAGGAATGTTCAGAGACAACTAATGAA
TCA----------

>FCL-AA01Z.Seq
----------TTCCTTCATCTTCCCTCATTCCATGCAAACGTACTTTCACTTTAAATACT
TCCTCTGAAAATCAAGATAATCTTAATATGGCAGTCTATCAAGGTAGTGCACCACTCGCC
AAAGATAATCAATTGGTTTCACGTTTCATCTTTAAATCTTCTCCAAATCCAATCGATGTC
ACTTTTGAAATTGATAATAACAATACTTTATTAATTAAATCCGAACAAGCAAATTATTCA
ATTAAATTTTAAAAATTAAACTATTAAATATTATAAAAAAATTCAAATAATAATAATAAT
AATAATAATTAATAACAAATAAATAAAAATAAAAATAAAAAAAAAATAAAAAAAAGTAAT
ACACGGTAAATAATTTGATTTTCAAATTAAAAAATAGTATTAAAAAAAAAAAAAAAAATT
A

>FCL-AA01P.Seq
GCAAATAATAATATTATGGGTATTGACTTTGGTACACATTTCGCATGTGTTGGTATTTTC
AAGAATGAAAGAATTGAAATCTGTCCAAATCAACAAGGTAACAGAACTACTCCATCAGTT
GTTTCATTCGTTGGTGACGATAAATTAGTTGGTGATGAAGCTAAAGCACAAATGGATAGA
AATCCATTAAATACAATTTATGATGTTAAAAGATTATTAGGTAGAAAAACTACTGACGAA
TTATTTGATCATGAAGTTAAAAAATTATCATTTAAAGTTACAACATATGAAGATAATAAT
GAAAAGATTGAATTTCAAGTTAACTATAAATCAAATGTTGTTACATTAACACCAATTGAA
ATTGCAACCAGTATTTTAGAACAAATTAAACATACTGCAGAGACATTTATTGGTGGTGAA
TCAATTAAGAAGGCCGTTATATCAGTACCAACAGATTTCACAGAGAAACAAAGAAACGAT
TTGAAAGAGGCAGCCACAGCAGCAGGTATTACAGTTGTTAGAATGATTCATGAACATTCA
GCAGTTGCATTAGCATATGGTTATGATCAAGTTAAGGAATGTTCAGAGACAACTAATGAA
TCA----------TTCCTTCATCTTCCCTCATTCCATGCAAACGTACTTTCACTTTAAAT
ACTTCCTCTGAAAATCAAGATAATCTTAATATGGCAGTCTATCAAGGTAGTGCACCACTC
GCCAAAGATAATCAATTGGTTTCACGTTTCATCTTTAAATCTTCTCCAAATCCAATCGAT
GTCACTTTTGAAATTGATAATAACAATACTTTATTAATTAAATCCGAACAAGCAAATTAT
TCAATTAAATTTTAAAAATTAAACTATTAAATATTATAAAAAAATTCAAATAATAATAAT
AATAATAATAATTAATAACAAATAAATAAAAATAAAAATAAAAAAAAAATAAAAAAAAGT
AATACACGGTAAATAATTTGATTTTCAAATTAAAAAATAGTATTAAAAAAAAAAAAAAAA
ATTA