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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
FC-BT01
Library FC
(Link to library)
Clone ID FC-BT01
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID FC-BT01E
(Link to Original site)
Representative DNA sequence 
>FC-BT01 (FC-BT01Q) /CSM/FC/FC-BT/FC-BT01Q.Seq.d/
TGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAA
ACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCAC
CACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCC
ATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTG
AAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCG
CCGTCGTCTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCCATCTCGTCCAATGT
GTGTTGAATCATTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGAC
AAAAAATTGCCGTCGGTGTCATCAAATCACCCGTCAAGAAAGCCCCAGGTAAAGCAGGTG
ATAAGAAAGGTGCTGCTGCTCATCAAAGAAGAAATAAATAATTTGAAAAAAAATTTTATA
ATTATTCTAAAAATAAAAAAAAAAATAATAAAAAAGAGCTTTTTAAAAATAAAAA
sequence update 1998. 5.14
Translated Amino Acid sequence 
HHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIH
AGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMC
VESFTEYPPLGRFAVRDMRQKIAVGVIKSPVKKAPGKAGDKKGAAAHQRRNK*fekkfyn
ysknkkknnkkelfknk
Translated Amino Acid sequence (All Frames) 
Frame A:
citnnsqkpvqvtm*vstlktfqskklkevwslvtpktihhkklknsllklssstiqvks
mlvthqs*ivtlltlpvnslkssiklivvlvpslpkkvlppss*rmvmllwsn*phlvqc
vlnhslnthh*vvspsei*dkklpsvssnhpsrkpqvkqvirkvlllikeeinnlkknfi
iilkikkkiikksflkik


Frame B:
asrttprspsr*qcrfqr*krfsqrn*krygrw*lqkrsttrn*kircssyrpqpsrsnp
cwlltslrlshcshcl*ih*nrr*s*ssywcrrcqrrycrrrlkew*ccygrinpissnv
c*iih*iptirsfrrqryetkncrrchqitrqespr*sr**ercccsskkk*ii*kkil*
lf*k*kkk**kraf*k*k


Frame C:
HHEQLPEARPGDNVGFNVKNVSVKEIKRGMVAGDSKNDPPQETEKFVAQVIVLNHPGQIH
AGYSPVLDCHTAHIACKFTEIVDKVDRRTGAVVAKEGTAAVVLKNGDAAMVELTPSRPMC
VESFTEYPPLGRFAVRDMRQKIAVGVIKSPVKKAPGKAGDKKGAAAHQRRNK*fekkfyn
ysknkkknnkkelfknk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FC-BT01 (FC-BT01Q) /CSM/FC/FC-BT/FC-BT01Q.Seq.d/                     1041   0.0
SLC814 (SLC814Q) /CSM/SL/SLC8-A/SLC814Q.Seq.d/                        995   0.0
SLA634 (SLA634Q) /CSM/SL/SLA6-B/SLA634Q.Seq.d/                        995   0.0
SLA633 (SLA633Q) /CSM/SL/SLA6-B/SLA633Q.Seq.d/                        995   0.0
SFG782 (SFG782Q) /CSM/SF/SFG7-D/SFG782Q.Seq.d/                        995   0.0
FC-BQ16 (FC-BQ16Q) /CSM/FC/FC-BQ/FC-BQ16Q.Seq.d/                      995   0.0
FC-BP24 (FC-BP24Q) /CSM/FC/FC-BP/FC-BP24Q.Seq.d/                      995   0.0
FC-BO15 (FC-BO15Q) /CSM/FC/FC-BO/FC-BO15Q.Seq.d/                      995   0.0
FC-BG17 (FC-BG17Q) /CSM/FC/FC-BG/FC-BG17Q.Seq.d/                      995   0.0
VFI867 (VFI867Q) /CSM/VF/VFI8-C/VFI867Q.Seq.d/                        993   0.0
own update 2001.11.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   954   0.0    2
X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   926   0.0    1
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   797   0.0    1
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   101   4e-67  6
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   101   5e-67  6
AX109678|AX109678.1 Sequence 411 from Patent WO0123604.           101   5e-60  6
AX109677|AX109677.1 Sequence 410 from Patent WO0123604.           101   5e-60  6
AX109675|AX109675.1 Sequence 408 from Patent WO0123604.           101   2e-59  6
AX109676|AX109676.1 Sequence 409 from Patent WO0123604.           101   2e-59  6
AX109674|AX109674.1 Sequence 407 from Patent WO0123604.           101   2e-59  6
dna update 2003.12.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   301   7e-81
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   301   7e-81
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   301   7e-81
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   232   5e-60
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   230   2e-59
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   229   3e-59
AY582829_1(AY582829|pid:none) Acanthamoeba culbertsoni ef1a gene...   229   4e-59
AB326305_1(AB326305|pid:none) Solea senegalensis SseEF1A4 mRNA f...   228   1e-58
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   228   1e-58
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   227   2e-58
protein update 2009. 7. 3
PSORT 

 psg: 0.75  gvh: 0.50  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for FC-BT01 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID FC-BT01E
Full length Seq. 
>FC-BT01E.Seq
TGCATCACGAACAACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAA
ACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCAC
CACAAGAAACTGAAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCC
ATGCTGGTTACTCACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTG
AAATCGTCGATAAAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCG
CCGTCGTCTTAAAGAATGGTGATGCTGCTATGGTCGAATTAACCCCATCTCGTCCAATGT
GTGTTGAATCATTCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGAC
AAAAAATTGCCGTCGGTGTCATCAAATCACCCGTCAAGAAAGCCCCAGGTAAAGCAGGTG
ATAAGAAAGGTGCTGCTGCTCATCAAAGAAGAAATAAATAATTTGAAAAAAAATTTTATA
ATTATTCTAAAAATAAAAAAAAAAATAATAAAAAAGAGCTTTTTAAAAATAAAAA
Length of full length seq. 595