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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH744
Library AF
(Link to library)
Clone ID AFH744
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFH744P
(Link to Original site)
Representative DNA sequence 
>AFH744 (AFH744Q) /CSM/AF/AFH7-B/AFH744Q.Seq.d/
TAGATTCTGATAATTTCCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTT
ATGCCCCATGGTGCGGTCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAAC
AATTATCAGCCAACAAAAAGATTGCAATTGCAAAAGTTGATTGTACTCAACATGAACAAC
TCTGTAAACAAAATAAAGTTCAAGGTTATCCAACTTTAGTTGTTTTCAAGAATGGTAAAG
CTGAACCATATGAAGGTGATAGAACCACTAAATCAATTGTTCAAACTTTAGAAGAAGAAT
TAAAACCAACTATTTCAACTTTAGAATCAAATGAAGATATTGAAGAATTTAAAAAACAAC
ACCCAATTAGTGTTGTTGGTTTCTTTGATAATGATCATGATGATAGATTTAAATTATTTA
GTGAATTAGCAGGAAATAATAAGAAATCAGCTAAATTTGCCGTTGTAATTGATAAAGATT
TCTCAAAGGAACATGTTGAATCCACACCAAACGTTGTTTTATTTAGAAGTTTTGATGAAC
CAAXXXXXXXXXXCCACTCAAGATANCACTCAAGTTCTCGAAGATGTAAAGAAAATCGCT
ACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAA
GCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCC
AATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAA
TGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCA
GAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTA
GACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAAT
CTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATC
GTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCA
ACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGA
AATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTAC
CATCATCTCAAACTGANATAATGTNAATCTAAAAAAGATTCTTCGCTAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
DSDNFHNSVSEHDVTLVMFYAPWCGHCKTLKPLYEEAAKQLSANKKIAIAKVDCTQHEQL
CKQNKVQGYPTLVVFKNGKAEPYEGDRTTKSIVQTLEEELKPTISTLESNEDIEEFKKQH
PISVVGFFDNDHDDRFKLFSELAGNNKKSAKFAVVIDKDFSKEHVESTPNVVLFRSFDEP
---

---TQDXTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANK
ARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDS
PKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTI
MLFKADDKENPISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKLX*cxskkdssl
Translated Amino Acid sequence (All Frames) 
Frame A:
*iliisiiqflnmt*lllcfmphgavivkl*nhymkkllnnyqptkrlqlqklivlnmnn
svnkikfkviql*lfsrmvklnhmkvieplnqlfkl*kkn*nqlfql*nqmkilknlknn
tqlvllvslimimmidlnylvn*qeiirnqlnlpl*likisqrnmlnphqtlfylevlmn
q---

---plkixlkfskm*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpi
klvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*t
hqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqp
scssklmtkkiqslmkvkemii*tllnsfkitlp*iqitiisn*xnvnlkkilr*

Frame B:
rf**fp*fsf*t*rnscyvlcpmvrsl*nfktii*rsc*tiisqqkdcncks*lyst*tt
l*tk*ssrlsnfscfqew*s*ti*r**nh*incsnfrrriktnyfnfrik*ry*ri*ktt
pn*ccwfl***s***i*ii**isrk**eis*icrcn**rflkgtc*ihtkrcfi*kf**t
---

---hsrxhsssrrckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*
sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrl
tkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnh
halqs**qrksnll*rskk*syelc*ihsr*rchefklpssqtximxi*krffa

Frame C:
DSDNFHNSVSEHDVTLVMFYAPWCGHCKTLKPLYEEAAKQLSANKKIAIAKVDCTQHEQL
CKQNKVQGYPTLVVFKNGKAEPYEGDRTTKSIVQTLEEELKPTISTLESNEDIEEFKKQH
PISVVGFFDNDHDDRFKLFSELAGNNKKSAKFAVVIDKDFSKEHVESTPNVVLFRSFDEP
---

---TQDXTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANK
ARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDS
PKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTI
MLFKADDKENPISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKLX*cxskkdssl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH744 (AFH744Q) /CSM/AF/AFH7-B/AFH744Q.Seq.d/                       2399   0.0
AFJ889 (AFJ889Q) /CSM/AF/AFJ8-D/AFJ889Q.Seq.d/                       1348   0.0
AFJ339 (AFJ339Q) /CSM/AF/AFJ3-B/AFJ339Q.Seq.d/                       1340   0.0
AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1338   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1338   0.0
AFI881 (AFI881Q) /CSM/AF/AFI8-D/AFI881Q.Seq.d/                       1334   0.0
AFK319 (AFK319Q) /CSM/AF/AFK3-A/AFK319Q.Seq.d/                       1332   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1332   0.0
AFJ720 (AFJ720Q) /CSM/AF/AFJ7-A/AFJ720Q.Seq.d/                       1332   0.0
AFK889 (AFK889Q) /CSM/AF/AFK8-D/AFK889Q.Seq.d/                       1330   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end,single read.   599   e-167  1
CN206669|CN206669.1 Tor7097 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    78   4e-10  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
CN591465|CN591465.1 TTE00010235 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
CN597971|CN597971.1 TTE00009997 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
CN590755|CN590755.1 TTE00006978 Normalized large Tetrahymena thermophila cDNA, mRNA sequence.    74   6e-09  1
BM898120|BM898120.1 ph59g02.y1 Ostertagia ostertagi L4 pAMP1 v1 Ostertagia ostertagi cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    56   2e-08  2
BM897160|BM897160.1 ph57e02.y1 Ostertagia ostertagi L4 pAMP1 v1 Ostertagia ostertagi cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    56   3e-08  2
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.     70   9e-08  1
CK029548|CK029548.1 AGENCOURT_16620510 NIH_ZGC_7 Danio rerio cDNA clone IMAGE:7051751 5', mRNA sequence.    70   9e-08  1
dna update 2004. 6.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   174   7e-42
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   171   6e-41
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   170   8e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   3e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   164   6e-39
CP000585_262(CP000585|pid:none) Ostreococcus lucimarinus CCE9901...    94   7e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   8e-38
CR382131_125(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   152   3e-35
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   5e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   7e-35
protein update 2009. 4.22
PSORT 

 psg: 0.75  gvh: 0.43  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFH744 is cyt
5' end seq. ID AFH744F
5' end seq. 
>AFH744F.Seq
TAGATTCTGATAATTTCCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTT
ATGCCCCATGGTGCGGTCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAAC
AATTATCAGCCAACAAAAAGATTGCAATTGCAAAAGTTGATTGTACTCAACATGAACAAC
TCTGTAAACAAAATAAAGTTCAAGGTTATCCAACTTTAGTTGTTTTCAAGAATGGTAAAG
CTGAACCATATGAAGGTGATAGAACCACTAAATCAATTGTTCAAACTTTAGAAGAAGAAT
TAAAACCAACTATTTCAACTTTAGAATCAAATGAAGATATTGAAGAATTTAAAAAACAAC
ACCCAATTAGTGTTGTTGGTTTCTTTGATAATGATCATGATGATAGATTTAAATTATTTA
GTGAATTAGCAGGAAATAATAAGAAATCAGCTAAATTTGCCGTTGTAATTGATAAAGATT
TCTCAAAGGAACATGTTGAATCCACACCAAACGTTGTTTTATTTAGAAGTTTTGATGAAC
CAA----------
Length of 5' end seq. 543
3' end seq. ID AFH744Z
3' end seq. 
>AFH744Z.Seq
----------CCACTCAAGATANCACTCAAGTTCTCGAAGATGTAAAGAAAATCGCTACC
TCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCA
ACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAAT
AAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGG
ATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAA
TCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGAC
TCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTT
GCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTA
AAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACC
ATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAAT
GATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTACCAT
CATCTCAAACTGANATAATGTNAATCTAAAAAAGATTCTTCGCTAA
Length of 3' end seq. 696
Connected seq. ID AFH744P
Connected seq. 
>AFH744P.Seq
TAGATTCTGATAATTTCCATAATTCAGTTTCTGAACATGACGTAACTCTTGTTATGTTTT
ATGCCCCATGGTGCGGTCATTGTAAAACTTTAAAACCATTATATGAAGAAGCTGCTAAAC
AATTATCAGCCAACAAAAAGATTGCAATTGCAAAAGTTGATTGTACTCAACATGAACAAC
TCTGTAAACAAAATAAAGTTCAAGGTTATCCAACTTTAGTTGTTTTCAAGAATGGTAAAG
CTGAACCATATGAAGGTGATAGAACCACTAAATCAATTGTTCAAACTTTAGAAGAAGAAT
TAAAACCAACTATTTCAACTTTAGAATCAAATGAAGATATTGAAGAATTTAAAAAACAAC
ACCCAATTAGTGTTGTTGGTTTCTTTGATAATGATCATGATGATAGATTTAAATTATTTA
GTGAATTAGCAGGAAATAATAAGAAATCAGCTAAATTTGCCGTTGTAATTGATAAAGATT
TCTCAAAGGAACATGTTGAATCCACACCAAACGTTGTTTTATTTAGAAGTTTTGATGAAC
CAA----------CCACTCAAGATANCACTCAAGTTCTCGAAGATGTAAAGAAAATCGCT
ACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAA
GCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCC
AATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAA
TGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCA
GAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTA
GACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAAT
CTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATC
GTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCA
ACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGA
AATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATGAATTCAAATTAC
CATCATCTCAAACTGANATAATGTNAATCTAAAAAAGATTCTTCGCTAA
Length of connected seq. 1239
Full length Seq ID -
Full length Seq. -
Length of full length seq. -