NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH733
Library AF
(Link to library)
Clone ID AFH733
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFH733P
(Link to Original site)
Representative DNA sequence 
>AFH733 (AFH733Q) /CSM/AF/AFH7-B/AFH733Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTXXXXXXXXXXAAAGG
AAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCG
TTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAG
CCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATC
CAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAAGACA
ATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAAT
CTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAGCTAAAGATTTCT
NTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAG
CCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTG
AAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTA
AAACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFG---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEX
AKDFXEKTFVANQEAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTDXKKEEVKKSTKK
Translated Amino Acid sequence (All Frames) 
Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFG---

---kgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*mesk
enpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrxrlcrx
s*rfl*kdlccqprsrkenv*rprsrqs*xekes*rkiscrksc*krsrrs**rrrrsr*
rrfs*n*xqkrrsqkinqks

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplcl---

---KEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAK
KIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVXDSVEX
AKDFXEKTFVANQEAEKKMFDDLEAAKAEXRKKADEKLAAEKAAEKEAEEADEEEEEVAE
EDLVKTDXKKEEVKKSTKK

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvw---

---rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqr
ksqiqntkvngxth*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlsxk
lkisxkrpllptkkpkrkclttskppklxrerkltkn*lpkkllkkkpkklmkkkkkslk
ki*lklmxkkkksknqpkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        868   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        868   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        868   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        868   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        868   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        868   0.0
VFE671 (VFE671Q) /CSM/VF/VFE6-C/VFE671Q.Seq.d/                        868   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        868   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        868   0.0
VFD449 (VFD449Q) /CSM/VF/VFD4-C/VFD449Q.Seq.d/                        868   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   868   0.0    4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   7e-19  2
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    74   1e-11  3
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   1e-10  2
BT013428|BT013428.1 Lycopersicon esculentum clone 132068F, mRNA sequence.    74   3e-10  2
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   5e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   5e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74   5e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74   5e-09  1
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    74   5e-09  1
dna update 2004. 6.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   250   6e-65
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   159   1e-37
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   159   1e-37
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   159   2e-37
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   158   3e-37
AC003114_8(AC003114|pid:none) Arabidopsis thaliana chromosome 1 ...   158   3e-37
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   158   3e-37
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   158   3e-37
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   158   3e-37
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   157   7e-37
protein update 2009. 4.22
PSORT 

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for AFH733 is exc
5' end seq. ID AFH733F
5' end seq. 
>AFH733F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGT----------
Length of 5' end seq. 465
3' end seq. ID AFH733Z
3' end seq. 
>AFH733Z.Seq
----------AAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATA
TCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACG
ACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAA
AGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAG
AATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAAT
TATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAN
AAGCTAAAGATTTCTNTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGT
TTGACGACCTCGAAGCCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTG
CCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTG
AAGAAGATTTAGTTAAAACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAG
T
Length of 3' end seq. 591
Connected seq. ID AFH733P
Connected seq. 
>AFH733P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGT----------AAAGG
AAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCG
TTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAG
CCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATC
CAGAATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAAGACA
ATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAAT
CTGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAANAAGCTAAAGATTTCT
NTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAG
CCGCCAAAGCTGAANAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTG
AAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTA
AAACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT
Length of connected seq. 1056
Full length Seq ID -
Full length Seq. -
Length of full length seq. -