>FC-BH12 (FC-BH12Q) /CSM/FC/FC-BH/FC-BH12Q.Seq.d/
CGATAATACCAATTGAAATTGTCCATGGTAGAAATGTTTGGAAGAATGATTTAACTAAAT
CATTAGTTACCTTAAAGAATATTATCGCCAAAACTGGTAGTGATAGAATTTTATTGGCAC
CATCTTGTTCATTATTACATTGTCCACATTCATTAACTCGTGAAATTGGTAGAGTTGAAA
GTCAAGTCTTAGATTGGTTAGCTTTCTCATTAGAGAAATTAAAAGAAATTTCTTTCCTTA
CCTACGCTCTTAATAAATGTACTTTTACCACTGAAAATACTATTACTTTCCCAGAAGATA
CTTGTTCAAAAATTAAAGAATATTATCAATTAAATAAAGAATCAAATCAAAAGAGAAGAG
AATCAAAATTAATTCATAATGATATCGTTAAAAAGAGAGTTACTGAAATTACTCCATCAA
TGTTAAAACGTGAAAATCCATTCCCAGTTCGTCGTGAAGCCCAAAGAAAAAGATTAACTA
CTCTTCCAGCATTATTCCCAACCACTACTATTGGTTCATTCCCACAAACTAAAGAAGTCC
GTTTAGCAAGAAGTAACTTTAAATCAAAGAAAATCACACCAGAAGCTTATGATCAATTCA
TTCGTGATGAAATTAGAAAATGTATTAAAGTTCAAGAAGAATGTGAATTGGATGTTTTAG
TACATGGTGAXXXXXXXXXXTAAAGGTATTGCTTGTATTCAAATCGATGAACCAGCAATT
CGTGAAGGTCTTCCATTGCGTTTATCCGATTGGAATCAATATCTTAAATGGGCAATTGAC
TCTTTCTTATTATCAAGCACTGGTGTTAAAGATAGTACTCAAATTCACTCCCATATGTGT
TATTCAGATTTCAATGATATCTTTGAATCAATTCAAAGAATGGATACTGATGTTTTAACT
ATTGAAAATTCAAAATCTGATCTCAAACTCTTAAAAGCTTTTGAAAAATATGGTTACACA
AATGAAATTGGTCCAGGTCTCTATGATATTCACTCTCCAAGAATACCATCTGTTGAAGAT
ATGAAAGATCGTGTTGAACAAATGCTTAAATATCTCTCAACTAATCTCTTATGGATTAAT
CCAGATTGTGGTCTTAAAACTAGCCAACCAGCCACTACAAGATTAGCTTCCTTAATATGG
TTAAAGTTGCTAAACAATTTAGAGAAATTTATGCTAAAAAGAATAAAAATATAAACTAAT
AAATAAAATAAATAAAATAAAAAAAATATATTATTTAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA

IIPIEIVHGRNVWKNDLTKSLVTLKNIIAKTGSDRILLAPSCSLLHCPHSLTREIGRVES
QVLDWLAFSLEKLKEISFLTYALNKCTFTTENTITFPEDTCSKIKEYYQLNKESNQKRRE
SKLIHNDIVKKRVTEITPSMLKRENPFPVRREAQRKRLTTLPALFPTTTIGSFPQTKEVR
LARSNFKSKKITPEAYDQFIRDEIRKCIKVQEECELDVLVHG---

---KGIACIQIDEPAIREGLPLRLSDWNQYLKWAIDSFLLSSTGVKDSTQIHSHMCYSDF
NDIFESIQRMDTDVLTIENSKSDLKLLKAFEKYGYTNEIGPGLYDIHSPRIPSVEDMKDR
VEQMLKYLSTNLLWINPDCGLKTSQPATTRLASLIWLKLLNNLEKFMLKRIKI*tnk*nk
*nkknilfkkkkkkkkkkk

Frame A:
r*yqlklsmvemfgrmi*lnh*lp*rilspklvviefywhhlvhyyivhih*lvklvelk
vks*ig*lsh*rn*kkflslptllinvllplkilllsqkilvqklkniin*iknqikree
nqn*fimislkrellkllhqc*nvkihsqfvvkpkekd*llfqhysqplllvhshklkks
v*qevtlnqrkshqklminsfvmklenvlkfkknvnwmf*ymv---

---*ryclysnr*tsns*rssiafirlesis*mgn*lfliikhwc*r*ysnslpyvlfrf
q*yl*inskngy*cfny*kfki*sqtlksf*kiwlhk*nwsrsl*yslskntic*ryers
c*tna*isln*slmd*srlws*n*ptshykisflnmvkvakqfreiyakknknin**ik*
ik*kkyii*kkkkkkkkkkk

Frame B:
dntn*ncpw*kclee*fn*iisylkeyyrqnw***nfigtilfiitlstfins*nw*s*k
sslrlvsflireikrnffpylrs**myfyh*kyyyfprrylfkn*rilsik*rikskekr
ikins**yr*kesy*nysinvkt*ksipsss*spkkkinysssiipnhyywfiptn*rsp
fskk*l*ikenhtrsl*sihs**n*kmy*ssrrm*igcfstw*---

---KGIACIQIDEPAIREGLPLRLSDWNQYLKWAIDSFLLSSTGVKDSTQIHSHMCYSDF
NDIFESIQRMDTDVLTIENSKSDLKLLKAFEKYGYTNEIGPGLYDIHSPRIPSVEDMKDR
VEQMLKYLSTNLLWINPDCGLKTSQPATTRLASLIWLKLLNNLEKFMLKRIKI*tnk*nk
*nkknilfkkkkkkkkkkk

Frame C:
IIPIEIVHGRNVWKNDLTKSLVTLKNIIAKTGSDRILLAPSCSLLHCPHSLTREIGRVES
QVLDWLAFSLEKLKEISFLTYALNKCTFTTENTITFPEDTCSKIKEYYQLNKESNQKRRE
SKLIHNDIVKKRVTEITPSMLKRENPFPVRREAQRKRLTTLPALFPTTTIGSFPQTKEVR
LARSNFKSKKITPEAYDQFIRDEIRKCIKVQEECELDVLVHG---

---kvllvfksmnqqfvkvfhcvypiginilngqltlsyyqalvlkivlkftpicviqis
mislnqfkewilmf*llkiqnlisns*kllknmvtqmklvqvsmiftlqeyhllki*kiv
lnkclnisqlisygliqivvlklanqplqd*lp*yg*sc*ti*rnlc*ke*kyklinkin
kikkiyylkkkkkkkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FC-BH12 (FC-BH12Q) /CSM/FC/FC-BH/FC-BH12Q.Seq.d/                     2268   0.0
FC-393 (FC-393Q) /CSM/CG/CG-FC/FC-393Q.Seq.d/                        2268   0.0
VHO891 (VHO891Q) /CSM/VH/VHO8-D/VHO891Q.Seq.d/                        910   0.0
VHO802 (VHO802Q) /CSM/VH/VHO8-A/VHO802Q.Seq.d/                        910   0.0
VHH834 (VHH834Q) /CSM/VH/VHH8-B/VHH834Q.Seq.d/                        910   0.0
FCL-AB22 (FCL-AB22Q) /CSM/FCL/FCL-AB/FCL-AB22Q.Seq.d/                 910   0.0
VHK882 (VHK882Q) /CSM/VH/VHK8-D/VHK882Q.Seq.d/                        904   0.0
VHO272 (VHO272Q) /CSM/VH/VHO2-C/VHO272Q.Seq.d/                        896   0.0
VHO408 (VHO408Q) /CSM/VH/VHO4-A/VHO408Q.Seq.d/                        878   0.0
VHL170 (VHL170Q) /CSM/VH/VHL1-C/VHL170Q.Seq.d/                        868   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL403248|AL403248.1 T7 end of clone AT0AA007E03 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    62  2e-12  2
U32843|U32843.1 Haemophilus influenzae Rd section 158 of 163 of the complete genome.    44  5e-09  4
AX601846|AX601846.1 Sequence 32 from Patent WO02092626.           40  3e-08  4
U15099|U15099.1 Saccharomyces cerevisiae cobalamin-independent methionine synthase (MET6) gene, complete cds.    40  7e-08  4
U32508|U32508.1 Saccharomyces cerevisiae N5-methyltetrahydrofolate homocysteine methyltransferase (MET6) gene and aspartyl protease gene, complete cds.    40  3e-07  4
AF220054|AF220054.1 Coffea arabica methionine synthase mRNA, partial cds.    66  1e-06  1
BQ409914|BQ409914.1 GA__Ed0025H06r Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ed0025H06r, mRNA sequence.    50  2e-04  2
U18839|U18839.1 Saccharomyces cerevisiae chromosome V cosmids 9747, 8198, 9781, and lambda clones 3612 and 6052.    40  3e-04  4
BF276576|BF276576.1 GA__Eb0029H23f Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Eb0029H23f, mRNA sequence.    58  3e-04  1
BQ415319|BQ415319.1 GA__Ed0097G05r Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ed0097G05r, mRNA sequence.    58  3e-04  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X49) RecName: Full=5-methyltetrahydropteroyltriglutamate--ho...   406   e-112
AF226997_1(AF226997|pid:none) Cladosporium fulvum methionine syn...   196   1e-48
CP000109_2179(CP000109|pid:none) Thiomicrospira crunogena XCL-2,...   195   3e-48
(Q9UT19) RecName: Full=Probable 5-methyltetrahydropteroyltriglut...   194   4e-48
(Q058E7) RecName: Full=5-methyltetrahydropteroyltriglutamate--ho...   194   7e-48
(A6V1K5) RecName: Full=5-methyltetrahydropteroyltriglutamate--ho...   192   3e-47
AF275676_1(AF275676|pid:none) Aspergillus nidulellus cobalamin-i...   189   6e-47
CP001339_606(CP001339|pid:none) Thioalkalivibrio sp. HL-EbGR7, c...   190   8e-47
(Q2KYF3) RecName: Full=5-methyltetrahydropteroyltriglutamate--ho...   190   8e-47
(A9N9C7) RecName: Full=5-methyltetrahydropteroyltriglutamate--ho...   189   2e-46

 psg: 0.56  gvh: 0.57  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.19  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: extracellular, including cell wall

>> prediction for FC-BH12 is nuc

>FC-BH12F.Seq
CGATAATACCAATTGAAATTGTCCATGGTAGAAATGTTTGGAAGAATGATTTAACTAAAT
CATTAGTTACCTTAAAGAATATTATCGCCAAAACTGGTAGTGATAGAATTTTATTGGCAC
CATCTTGTTCATTATTACATTGTCCACATTCATTAACTCGTGAAATTGGTAGAGTTGAAA
GTCAAGTCTTAGATTGGTTAGCTTTCTCATTAGAGAAATTAAAAGAAATTTCTTTCCTTA
CCTACGCTCTTAATAAATGTACTTTTACCACTGAAAATACTATTACTTTCCCAGAAGATA
CTTGTTCAAAAATTAAAGAATATTATCAATTAAATAAAGAATCAAATCAAAAGAGAAGAG
AATCAAAATTAATTCATAATGATATCGTTAAAAAGAGAGTTACTGAAATTACTCCATCAA
TGTTAAAACGTGAAAATCCATTCCCAGTTCGTCGTGAAGCCCAAAGAAAAAGATTAACTA
CTCTTCCAGCATTATTCCCAACCACTACTATTGGTTCATTCCCACAAACTAAAGAAGTCC
GTTTAGCAAGAAGTAACTTTAAATCAAAGAAAATCACACCAGAAGCTTATGATCAATTCA
TTCGTGATGAAATTAGAAAATGTATTAAAGTTCAAGAAGAATGTGAATTGGATGTTTTAG
TACATGGTGA----------

>FC-BH12Z.Seq
----------TAAAGGTATTGCTTGTATTCAAATCGATGAACCAGCAATTCGTGAAGGTC
TTCCATTGCGTTTATCCGATTGGAATCAATATCTTAAATGGGCAATTGACTCTTTCTTAT
TATCAAGCACTGGTGTTAAAGATAGTACTCAAATTCACTCCCATATGTGTTATTCAGATT
TCAATGATATCTTTGAATCAATTCAAAGAATGGATACTGATGTTTTAACTATTGAAAATT
CAAAATCTGATCTCAAACTCTTAAAAGCTTTTGAAAAATATGGTTACACAAATGAAATTG
GTCCAGGTCTCTATGATATTCACTCTCCAAGAATACCATCTGTTGAAGATATGAAAGATC
GTGTTGAACAAATGCTTAAATATCTCTCAACTAATCTCTTATGGATTAATCCAGATTGTG
GTCTTAAAACTAGCCAACCAGCCACTACAAGATTAGCTTCCTTAATATGGTTAAAGTTGC
TAAACAATTTAGAGAAATTTATGCTAAAAAGAATAAAAATATAAACTAATAAATAAAATA
AATAAAATAAAAAAAATATATTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
A

>FC-BH12P.Seq
CGATAATACCAATTGAAATTGTCCATGGTAGAAATGTTTGGAAGAATGATTTAACTAAAT
CATTAGTTACCTTAAAGAATATTATCGCCAAAACTGGTAGTGATAGAATTTTATTGGCAC
CATCTTGTTCATTATTACATTGTCCACATTCATTAACTCGTGAAATTGGTAGAGTTGAAA
GTCAAGTCTTAGATTGGTTAGCTTTCTCATTAGAGAAATTAAAAGAAATTTCTTTCCTTA
CCTACGCTCTTAATAAATGTACTTTTACCACTGAAAATACTATTACTTTCCCAGAAGATA
CTTGTTCAAAAATTAAAGAATATTATCAATTAAATAAAGAATCAAATCAAAAGAGAAGAG
AATCAAAATTAATTCATAATGATATCGTTAAAAAGAGAGTTACTGAAATTACTCCATCAA
TGTTAAAACGTGAAAATCCATTCCCAGTTCGTCGTGAAGCCCAAAGAAAAAGATTAACTA
CTCTTCCAGCATTATTCCCAACCACTACTATTGGTTCATTCCCACAAACTAAAGAAGTCC
GTTTAGCAAGAAGTAACTTTAAATCAAAGAAAATCACACCAGAAGCTTATGATCAATTCA
TTCGTGATGAAATTAGAAAATGTATTAAAGTTCAAGAAGAATGTGAATTGGATGTTTTAG
TACATGGTGA----------TAAAGGTATTGCTTGTATTCAAATCGATGAACCAGCAATT
CGTGAAGGTCTTCCATTGCGTTTATCCGATTGGAATCAATATCTTAAATGGGCAATTGAC
TCTTTCTTATTATCAAGCACTGGTGTTAAAGATAGTACTCAAATTCACTCCCATATGTGT
TATTCAGATTTCAATGATATCTTTGAATCAATTCAAAGAATGGATACTGATGTTTTAACT
ATTGAAAATTCAAAATCTGATCTCAAACTCTTAAAAGCTTTTGAAAAATATGGTTACACA
AATGAAATTGGTCCAGGTCTCTATGATATTCACTCTCCAAGAATACCATCTGTTGAAGAT
ATGAAAGATCGTGTTGAACAAATGCTTAAATATCTCTCAACTAATCTCTTATGGATTAAT
CCAGATTGTGGTCTTAAAACTAGCCAACCAGCCACTACAAGATTAGCTTCCTTAATATGG
TTAAAGTTGCTAAACAATTTAGAGAAATTTATGCTAAAAAGAATAAAAATATAAACTAAT
AAATAAAATAAATAAAATAAAAAAAATATATTATTTAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA