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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH712
Library AF
(Link to library)
Clone ID AFH712
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14943-1
Original site URL
Representative seq. ID AFH712P
(Link to Original site)
Representative DNA sequence 
>AFH712 (AFH712Q) /CSM/AF/AFH7-A/AFH712Q.Seq.d/
AAAACATCACTTATCTTTATAAATAAATAAATAAATAAATTTATACAATTTACAAAGAAT
AATATAAAACTAGATATTTAAATTTTTGATAATAAAAACACTTAATAGATTTTGTATTTA
AAATTTAAATAGAAATTAAAGATGGCAACTAAAAATAAACCAAATTTATCAGTTCAAGAT
GTAAAGTTATTGAATGTCAATGAATTGACCCCATTAACACCAAATGTTATTAGTAGACAA
GCAACAATTAATATTGGTACCATTGGTCATGTAGCTCATGGTAAAAGTACTGTAGTTAAA
GCAATCTCTGGTGTTTTGACAGTTAGATTTACATCAGAATTCAAACGTAATATTACAATT
AAATTAGGTTATGCAAATGCAAAGATTTATAAATGTGATAACCCACAATGTGAAAGACCA
GGTTGTTACAAATCAGCACGTTCAAATACTGAAGATAATCCACAATGTGAAAGACCAGGT
TGTGGTGGTAGAATGACTCTCTTACGTCATGTTTCTTTTGTAGATTGTCCAGGTCATGAT
GTTTTAATGGCAACTATGTTAAATGGTGCAGCAGTTATGGATGCAGCTCXXXXXXXXXXA
ATNAACTTCAANACNCTGNACCAGGTGGTTTAATTGGNGTTGGNACAAANATCGATCCAA
CCCTTTGCAAANCTGATCGTTTGGTTGGTCAAGTTTTAGGTTCAGTCGGTAAATTACCAN
AAATTTTNGTCGCTCTCGAAGTTAACTTTTTCCTCTTACGTANACTTTTAGGTGTTAAAT
CCGATGGTGATAAACAAAGTAAAGTTAAAAAATTATCCAAANAANATACCCTTATGGTTA
ACNTTGGTTCAACTAGNACTGGTTGTCGNGTCGTCNCTGTTAAACATGATTTAGCTAAAC
NTCAACNTTTAACNCCNGTTTGNTCTCAANAAGGTGAAAAAATCGCTTTATCAANAANAG
TCGATAAAAATTGGAGACTTATTGGTTGGGGTGAAATTAAAAAAGGGACCGTTCNCNCTA
ATTAATCNAAAAATAATAANGCNTTCNATTTAACTTAATGAAACATGNAATTTTCNAAAA
ATTTGCNCGTAAAAAAAAAAAATATTAAACCAAAAAAAATAAAAAATAAAAAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
ktslifink*inkfiqftknnikldi*ifdnknt**ilylkfk*KLKMATKNKPNLSVQD
VKLLNVNELTPLTPNVISRQATINIGTIGHVAHGKSTVVKAISGVLTVRFTSEFKRNITI
KLGYANAKIYKCDNPQCERPGCYKSARSNTEDNPQCERPGCGGRMTLLRHVSFVDCPGHD
VLMATMLNGAAVMDAA---

---NXLQXXXPGGLIGVGTXIDPTLCKXDRLVGQVLGSVGKLPXIXVALEVNFFLLRXLL
GVKSDGDKQSKVKKLSKXXTLMVNXGSTXTGCRVVXVKHDLAKXQXLTPVXSQXGEKIAL
SXXVDKNWRLIGWGEIKKGTVXXN*sknnxafxlt**nmxfsknlxvkkknikpkkiknk
k
Translated Amino Acid sequence (All Frames) 
Frame A:
ktslifink*inkfiqftknnikldi*ifdnknt**ilylkfk*KLKMATKNKPNLSVQD
VKLLNVNELTPLTPNVISRQATINIGTIGHVAHGKSTVVKAISGVLTVRFTSEFKRNITI
KLGYANAKIYKCDNPQCERPGCYKSARSNTEDNPQCERPGCGGRMTLLRHVSFVDCPGHD
VLMATMLNGAAVMDAA---

---NXLQXXXPGGLIGVGTXIDPTLCKXDRLVGQVLGSVGKLPXIXVALEVNFFLLRXLL
GVKSDGDKQSKVKKLSKXXTLMVNXGSTXTGCRVVXVKHDLAKXQXLTPVXSQXGEKIAL
SXXVDKNWRLIGWGEIKKGTVXXN*sknnxafxlt**nmxfsknlxvkkknikpkkiknk
k

Frame B:
khhlsl*ink*inlynlqrii*n*ifkfliiktlnrfci*nlnrn*rwqlkinqiyqfkm
*sy*msmn*ph*hqmllvdkqqlilvplvm*lmvkvl*lkqslvf*qldlhqnsnvilql
n*vmqmqrfinvithnvkdqvvtnqhvqilkiihnvkdqvvvve*lsyvmfll*ivqvmm
f*wqlc*mvqqlwmql---

---xnfxtlxqvv*lxlxqxsiqpfaxlivwlvkf*vqsvnyxkfxslskltfssyvxf*
vlnpmvinkvklknypxxiplwltlvqlxlvvxssllnmi*lnxnx*xxfxlxkvkksly
qxxsikigdllvgvklkkgpfxlinxkiixxsi*lnetxnfxkicx*kkkilnqkk*kik

Frame C:
nitylyk*ink*iytiyke*yktrylnf***khlidfvfki*ieikdgn*k*tkfissrc
kviecq*idpintkcy**tsnn*ywyhwscssw*kycs*snlwcfds*iyiriqt*yyn*
irlckckdl*m**ptm*ktrllqistfky*r*stm*ktrlww*ndsltscffcrlsrs*c
fngnyvkwcssygcs---

---xtsxxxtrwfnwxwxkxrsnplqx*sfgwssfrfsr*itxnfxrsrs*lfpltxtfr
c*irw**tk*s*kiiqxxypyg*xwfn*xwlsxrxc*t*fs*tstfnxxlxsxr*knrfi
xxsr*kletywlg*n*krdrsx*lixk**xxxfnlmkhxifxkfarkkkky*tkknkk*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH712 (AFH712Q) /CSM/AF/AFH7-A/AFH712Q.Seq.d/                       1772   0.0
VHF332 (VHF332Q) /CSM/VH/VHF3-B/VHF332Q.Seq.d/                       1041   0.0
VFN278 (VFN278Q) /CSM/VF/VFN2-D/VFN278Q.Seq.d/                       1041   0.0
VFG614 (VFG614Q) /CSM/VF/VFG6-A/VFG614Q.Seq.d/                       1041   0.0
VFF472 (VFF472Q) /CSM/VF/VFF4-C/VFF472Q.Seq.d/                       1041   0.0
SFB138 (SFB138Q) /CSM/SF/SFB1-B/SFB138Q.Seq.d/                       1041   0.0
CFI833 (CFI833Q) /CSM/CF/CFI8-B/CFI833Q.Seq.d/                       1041   0.0
AHA128 (AHA128Q) /CSM/AH/AHA1-B/AHA128Q.Seq.d/                       1041   0.0
AFJ717 (AFJ717Q) /CSM/AF/AFJ7-A/AFJ717Q.Seq.d/                       1041   0.0
AFB361 (AFB361Q) /CSM/AF/AFB3-C/AFB361Q.Seq.d/                       1041   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ010980|AJ010980.1 Dictyostelium discoideum mRNA for translation initiation factor 2  gamma, partial.   650   0.0    2
AE014817|AE014817.1 Plasmodium falciparum 3D7 chromosome 14 section 2 of 13 of the complete sequence.   105   2e-40  16
BI512080|BI512080.1 BB160008A10D08 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160008A10D08 5', mRNA sequence.    84   7e-39  3
BI515858|BI515858.1 BB160020B10C10 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160020B10C10 5', mRNA sequence.    84   1e-38  3
BI511038|BI511038.1 BB160004A20G11 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160004A20G11 5', mRNA sequence.    84   1e-38  3
CB615909|CB615909.1 AMGNNUC:NRDG1-00078-H9-A nrdg1 (10855) Rattus norvegicus cDNA clone nrdg1-00078-h9 5', mRNA sequence.   100   2e-29  3
CB585276|CB585276.1 AMGNNUC:NRDG1-00192-C8-A nrdg1 (10855) Rattus norvegicus cDNA clone nrdg1-00192-c8 5', mRNA sequence.   100   2e-29  3
CB576222|CB576222.1 AMGNNUC:NRDG1-00184-H12-A nrdg1 (10855) Rattus norvegicus cDNA clone nrdg1-00184-h12 5', mRNA sequence.   100   3e-29  3
BH136149|BH136149.1 ENTOF55TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    98   1e-27  5
BX665323|BX665323.1 Sus Scrofa library (scag) clone scag0006c.b.12, 5prim end (T7  primer).    84   9e-26  3
dna update 2004. 6.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ290961_3(AJ290961|pid:none) Clytus arietis partial Su(var)3-9 ...   227   5e-58
AJ715860_1(AJ715860|pid:none) Oniscus asellus mRNA for eukaryoti...   222   2e-56
AJ715864_1(AJ715864|pid:none) Forficula auricularia mRNA for euk...   220   6e-56
CR940347_847(CR940347|pid:none) Theileria annulata strain Ankara...   218   4e-55
AM270071_15(AM270071|pid:none) Aspergillus niger contig An04c010...   217   5e-55
AJ715862_1(AJ715862|pid:none) Lepismachilis sp. VK-2004 mRNA for...   216   1e-54
AJ715857_1(AJ715857|pid:none) Lithobius forficatus mRNA for euka...   214   3e-54
AJ715868_1(AJ715868|pid:none) Cercopis vulnerata mRNA for eukary...   214   6e-54
AY223199_1(AY223199|pid:none) Schistosoma japonicum clone ZZD207...   214   6e-54
AJ715861_1(AJ715861|pid:none) Allacma fusca eIF2g gene for eukar...   214   6e-54
protein update 2009. 4.22
PSORT 

 psg: 0.65  gvh: 0.47  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for AFH712 is cyt
5' end seq. ID AFH712F
5' end seq. 
>AFH712F.Seq
AAAACATCACTTATCTTTATAAATAAATAAATAAATAAATTTATACAATTTACAAAGAAT
AATATAAAACTAGATATTTAAATTTTTGATAATAAAAACACTTAATAGATTTTGTATTTA
AAATTTAAATAGAAATTAAAGATGGCAACTAAAAATAAACCAAATTTATCAGTTCAAGAT
GTAAAGTTATTGAATGTCAATGAATTGACCCCATTAACACCAAATGTTATTAGTAGACAA
GCAACAATTAATATTGGTACCATTGGTCATGTAGCTCATGGTAAAAGTACTGTAGTTAAA
GCAATCTCTGGTGTTTTGACAGTTAGATTTACATCAGAATTCAAACGTAATATTACAATT
AAATTAGGTTATGCAAATGCAAAGATTTATAAATGTGATAACCCACAATGTGAAAGACCA
GGTTGTTACAAATCAGCACGTTCAAATACTGAAGATAATCCACAATGTGAAAGACCAGGT
TGTGGTGGTAGAATGACTCTCTTACGTCATGTTTCTTTTGTAGATTGTCCAGGTCATGAT
GTTTTAATGGCAACTATGTTAAATGGTGCAGCAGTTATGGATGCAGCTC----------
Length of 5' end seq. 589
3' end seq. ID AFH712Z
3' end seq. 
>AFH712Z.Seq
----------AATNAACTTCAANACNCTGNACCAGGTGGTTTAATTGGNGTTGGNACAAA
NATCGATCCAACCCTTTGCAAANCTGATCGTTTGGTTGGTCAAGTTTTAGGTTCAGTCGG
TAAATTACCANAAATTTTNGTCGCTCTCGAAGTTAACTTTTTCCTCTTACGTANACTTTT
AGGTGTTAAATCCGATGGTGATAAACAAAGTAAAGTTAAAAAATTATCCAAANAANATAC
CCTTATGGTTAACNTTGGTTCAACTAGNACTGGTTGTCGNGTCGTCNCTGTTAAACATGA
TTTAGCTAAACNTCAACNTTTAACNCCNGTTTGNTCTCAANAAGGTGAAAAAATCGCTTT
ATCAANAANAGTCGATAAAAATTGGAGACTTATTGGTTGGGGTGAAATTAAAAAAGGGAC
CGTTCNCNCTAATTAATCNAAAAATAATAANGCNTTCNATTTAACTTAATGAAACATGNA
ATTTTCNAAAAATTTGCNCGTAAAAAAAAAAAATATTAAACCAAAAAAAATAAAAAATAA
AAAA
Length of 3' end seq. 534
Connected seq. ID AFH712P
Connected seq. 
>AFH712P.Seq
AAAACATCACTTATCTTTATAAATAAATAAATAAATAAATTTATACAATTTACAAAGAAT
AATATAAAACTAGATATTTAAATTTTTGATAATAAAAACACTTAATAGATTTTGTATTTA
AAATTTAAATAGAAATTAAAGATGGCAACTAAAAATAAACCAAATTTATCAGTTCAAGAT
GTAAAGTTATTGAATGTCAATGAATTGACCCCATTAACACCAAATGTTATTAGTAGACAA
GCAACAATTAATATTGGTACCATTGGTCATGTAGCTCATGGTAAAAGTACTGTAGTTAAA
GCAATCTCTGGTGTTTTGACAGTTAGATTTACATCAGAATTCAAACGTAATATTACAATT
AAATTAGGTTATGCAAATGCAAAGATTTATAAATGTGATAACCCACAATGTGAAAGACCA
GGTTGTTACAAATCAGCACGTTCAAATACTGAAGATAATCCACAATGTGAAAGACCAGGT
TGTGGTGGTAGAATGACTCTCTTACGTCATGTTTCTTTTGTAGATTGTCCAGGTCATGAT
GTTTTAATGGCAACTATGTTAAATGGTGCAGCAGTTATGGATGCAGCTC----------A
ATNAACTTCAANACNCTGNACCAGGTGGTTTAATTGGNGTTGGNACAAANATCGATCCAA
CCCTTTGCAAANCTGATCGTTTGGTTGGTCAAGTTTTAGGTTCAGTCGGTAAATTACCAN
AAATTTTNGTCGCTCTCGAAGTTAACTTTTTCCTCTTACGTANACTTTTAGGTGTTAAAT
CCGATGGTGATAAACAAAGTAAAGTTAAAAAATTATCCAAANAANATACCCTTATGGTTA
ACNTTGGTTCAACTAGNACTGGTTGTCGNGTCGTCNCTGTTAAACATGATTTAGCTAAAC
NTCAACNTTTAACNCCNGTTTGNTCTCAANAAGGTGAAAAAATCGCTTTATCAANAANAG
TCGATAAAAATTGGAGACTTATTGGTTGGGGTGAAATTAAAAAAGGGACCGTTCNCNCTA
ATTAATCNAAAAATAATAANGCNTTCNATTTAACTTAATGAAACATGNAATTTTCNAAAA
ATTTGCNCGTAAAAAAAAAAAATATTAAACCAAAAAAAATAAAAAATAAAAAA
Length of connected seq. 1123
Full length Seq ID -
Full length Seq. -
Length of full length seq. -