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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFH706
Library AF
(Link to library)
Clone ID AFH706
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U08694-1
Original site URL
Representative seq. ID AFH706P
(Link to Original site)
Representative DNA sequence 
>AFH706 (AFH706Q) /CSM/AF/AFH7-A/AFH706Q.Seq.d/
TTGGCCTACTGGAAATAAATAAATAAATAAAATGTCAGAAAAAGAAACAAATACAACATC
AACAGAAAATAAAGAAAAAGAAAAACAAGAACAAACTAATACAAATTCAACAACAGAATC
AACAAATAATCAAGTTGATGAAGAATATGAAAGACCAGGAAGATCAGAAGGTTTAGATGA
ATTTGGAATTCAATTAGATATTCAACAATCTGATCCAAATTCACCATTATATTCAGTGAA
AACATTTGAAGAATTAGGATTAAAACCAGAGTTATTAAAGGGTGTTTATGCAATGGGTTA
TAATAAACCATCTAAAATTCAAGAAGCAGCATTACCAATTATTATTCAATCACCAAATAA
TTTAATTGCACAATCACAATCAGGTACTGGTAAAACTGCTGCATTCACATTGGGTATGTT
AAATTGTGTTGATCCATCAATCAATGCACCACAAGCAATTTXXXXXXXXXXTCTGCAACA
TTTAGTATGGGAGTTGAGGAATTGATTAAAAAGATCGTTCAAGATCCATATACAAGTATA
AGATTAAAAAGACAAGAGTTATCTGTTGAAAAGATTCATCAATATTTTATAGATTGTGGT
AGTGAAGATAATAAAGCATTGATTCTATCAGACATCTATGGTTTCATATCAGTTGGTCAG
TCAATTGTATTCGTACATACAATTGCAACTGCAAAATCGGTACACCAAAAAATGGTTGAT
GAAGGTCATTCAGTTTCATTACTCTATGGTAAAGATTTAACAACAGAGGAACGTTTTAAA
CAAATTAAAGATTTCAAAGATGGTAAATCAAAAGTATTGATAACAACAAATGTTTTAGCT
AGAGGTATTGATATTCCACAAGTTTCATTGGTGATCAATTATGATGTACCATTGGATGAA
ATGGGTAAACCTGACCCAGTTCATTATCTTCATCGTATTGGTAGAGTTGGTAGATTCGGT
CGTTCTGGTGTTGCTCTTAGTTTTGTTTATGATCAACAATCAACAAATAAATTAATGAAT
ATTTCAACCCATCTTGGTGTACCTTTAAAAGAATTAAAATCTTCTGAAATTGAATCTTTA
GATGGTATCTTAAAAGGTATTAGAAATCAATTAACACCATTAAAAATTAAAAAAAAACAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
wptgnk*INKMSEKETNTTSTENKEKEKQEQTNTNSTTESTNNQVDEEYERPGRSEGLDE
FGIQLDIQQSDPNSPLYSVKTFEELGLKPELLKGVYAMGYNKPSKIQEAALPIIIQSPNN
LIAQSQSGTGKTAAFTLGMLNCVDPSINAPQAI---

---SATFSMGVEELIKKIVQDPYTSIRLKRQELSVEKIHQYFIDCGSEDNKALILSDIYG
FISVGQSIVFVHTIATAKSVHQKMVDEGHSVSLLYGKDLTTEERFKQIKDFKDGKSKVLI
TTNVLARGIDIPQVSLVINYDVPLDEMGKPDPVHYLHRIGRVGRFGRSGVALSFVYDQQS
TNKLMNISTHLGVPLKELKSSEIESLDGILKGIRNQLTPLKIKKKH
Translated Amino Acid sequence (All Frames) 
Frame A:
laywk*ink*nvrkrnkyninrk*rkrktrtn*ykfnnrink*ss**ri*ktrkirrfr*
iwnsirysti*skftiifseni*ririktrvikgclcngl**ti*nsrssitnyysitk*
fnctitiryw*nccihigyvklc*sinqcttsn---

---SATFSMGVEELIKKIVQDPYTSIRLKRQELSVEKIHQYFIDCGSEDNKALILSDIYG
FISVGQSIVFVHTIATAKSVHQKMVDEGHSVSLLYGKDLTTEERFKQIKDFKDGKSKVLI
TTNVLARGIDIPQVSLVINYDVPLDEMGKPDPVHYLHRIGRVGRFGRSGVALSFVYDQQS
TNKLMNISTHLGVPLKELKSSEIESLDGILKGIRNQLTPLKIKKKH

Frame B:
wptgnk*INKMSEKETNTTSTENKEKEKQEQTNTNSTTESTNNQVDEEYERPGRSEGLDE
FGIQLDIQQSDPNSPLYSVKTFEELGLKPELLKGVYAMGYNKPSKIQEAALPIIIQSPNN
LIAQSQSGTGKTAAFTLGMLNCVDPSINAPQAI---

---lqhlvwelrn*lkrsfkihiqv*d*kdksyllkrfinil*ivvvkiikh*fyqtsmv
syqlvsqlysyiqlqlqnrytkkwlmkviqfhysmvki*qqrnvlnklkiskmvnqky**
qqmf*levlifhkfhw*simmyhwmkwvnltqfiifivlvelvdsvvlvlllvlfminnq
qin**ifqpilvyl*kn*nllklnl*mvs*kvlein*hh*klkkn

Frame C:
glleink*ikcqkkkqiqhqqkikkkknknkliqiqqqnqqiiklmknmkdqedqkv*mn
lefn*ifnnliqihhyiq*khlkn*d*nqsy*rvfmqwviinhlkfkkqhyqllfnhqii
*lhnhnqvlvkllhshwvc*ivlihqsmhhkqf---

---cni*ygs*gid*kdrsrsiykykikktrvic*kdssifyrlw**r**sidsirhlwf
hiswsvncirtyncnckigtpkng**rsfsfitlw*rfnnrgtf*tn*rfqrw*iksidn
nkcfs*ry*ystsfigdql*ctig*ng*t*psslsssyw*sw*irsfwccs*fcl*stin
k*ineyfnpswctfkrikif*n*ifrwylkry*ksintikn*kkt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFH706 (AFH706Q) /CSM/AF/AFH7-A/AFH706Q.Seq.d/                       1927   0.0
AFC886 (AFC886Q) /CSM/AF/AFC8-D/AFC886Q.Seq.d/                       1265   0.0
SFJ390 (SFJ390Q) /CSM/SF/SFJ3-D/SFJ390Q.Seq.d/                       1245   0.0
CFG311 (CFG311Q) /CSM/CF/CFG3-A/CFG311Q.Seq.d/                       1241   0.0
VFM343 (VFM343Q) /CSM/VF/VFM3-B/VFM343Q.Seq.d/                        549   e-155
VFB308 (VFB308Q) /CSM/VF/VFB3-A/VFB308Q.Seq.d/                         50   8e-05
SHB305 (SHB305Q) /CSM/SH/SHB3-A/SHB305Q.Seq.d/                         42   0.018
AFJ849 (AFJ849Q) /CSM/AF/AFJ8-C/AFJ849Q.Seq.d/                         42   0.018
VFO234 (VFO234Q) /CSM/VF/VFO2-B/VFO234Q.Seq.d/                         40   0.073
VFN391 (VFN391Q) /CSM/VF/VFN3-D/VFN391Q.Seq.d/                         40   0.073
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF002677|AF002677.1 Dictyostelium discoideum DEAD-box RNA helicase (HelC) mRNA, complete cds.  1092   0.0    2
BP179955|BP179955.1 Bombyx mori cDNA, clone: ovS309A02f.           48   2e-14  3
CK522134|CK522134.1 rswea0_007252.y1 swe Bombyx mori cDNA, mRNA sequence.    48   5e-13  3
AV401150|AV401150.1 Bombyx mori cDNA clone:e96h0289.               48   5e-13  3
AU000564|AU000564.1 Bombyx mori cDNA, clone e40707.                48   7e-13  3
AV400446|AV400446.1 Bombyx mori cDNA clone:br--1252.               48   8e-13  3
AL407601|AL407601.1 T3 end of clone AV0AA003C07 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58   2e-10  2
AV401489|AV401489.1 Bombyx mori cDNA clone:e96h0796.               44   2e-10  3
BI452414|BI452414.1 GI10_G07 Glomus intraradices extra-radical mycelium Glomus intraradices cDNA clone GI10_G07 3', mRNA sequence.    52   5e-10  2
BI452308|BI452308.1 GI08_E11 Glomus intraradices extra-radical mycelium Glomus intraradices cDNA clone GI08_E11 3', mRNA sequence.    58   3e-08  2
dna update 2004. 6.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54TF8) RecName: Full=ATP-dependent RNA helicase ddx19;        ...   426   e-118
AF002677_1(AF002677|pid:none) Dictyostelium discoideum DEAD-box ...   365   1e-99
(Q6CJU1) RecName: Full=ATP-dependent RNA helicase DBP5;         ...   195   2e-48
FM992690_168(FM992690|pid:none) Candida dubliniensis CD36 chromo...   195   3e-48
(Q75C39) RecName: Full=ATP-dependent RNA helicase DBP5;         ...   195   3e-48
(A6ZNQ1) RecName: Full=ATP-dependent RNA helicase DBP5;         ...   190   9e-47
(A3GH91) RecName: Full=ATP-dependent RNA helicase DBP5;         ...   189   1e-46
(Q4P7Z8) RecName: Full=ATP-dependent RNA helicase DBP5;         ...   187   7e-46
(Q09747) RecName: Full=ATP-dependent RNA helicase dbp5;         ...   183   8e-45
(Q6FKN8) RecName: Full=ATP-dependent RNA helicase DBP5;         ...   180   7e-44
protein update 2009. 4.22
PSORT 

 psg: 0.63  gvh: 0.06  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.07  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 28.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFH706 is nuc
5' end seq. ID AFH706F
5' end seq. 
>AFH706F.Seq
TTGGCCTACTGGAAATAAATAAATAAATAAAATGTCAGAAAAAGAAACAAATACAACATC
AACAGAAAATAAAGAAAAAGAAAAACAAGAACAAACTAATACAAATTCAACAACAGAATC
AACAAATAATCAAGTTGATGAAGAATATGAAAGACCAGGAAGATCAGAAGGTTTAGATGA
ATTTGGAATTCAATTAGATATTCAACAATCTGATCCAAATTCACCATTATATTCAGTGAA
AACATTTGAAGAATTAGGATTAAAACCAGAGTTATTAAAGGGTGTTTATGCAATGGGTTA
TAATAAACCATCTAAAATTCAAGAAGCAGCATTACCAATTATTATTCAATCACCAAATAA
TTTAATTGCACAATCACAATCAGGTACTGGTAAAACTGCTGCATTCACATTGGGTATGTT
AAATTGTGTTGATCCATCAATCAATGCACCACAAGCAATTT----------
Length of 5' end seq. 461
3' end seq. ID AFH706Z
3' end seq. 
>AFH706Z.Seq
----------TCTGCAACATTTAGTATGGGAGTTGAGGAATTGATTAAAAAGATCGTTCA
AGATCCATATACAAGTATAAGATTAAAAAGACAAGAGTTATCTGTTGAAAAGATTCATCA
ATATTTTATAGATTGTGGTAGTGAAGATAATAAAGCATTGATTCTATCAGACATCTATGG
TTTCATATCAGTTGGTCAGTCAATTGTATTCGTACATACAATTGCAACTGCAAAATCGGT
ACACCAAAAAATGGTTGATGAAGGTCATTCAGTTTCATTACTCTATGGTAAAGATTTAAC
AACAGAGGAACGTTTTAAACAAATTAAAGATTTCAAAGATGGTAAATCAAAAGTATTGAT
AACAACAAATGTTTTAGCTAGAGGTATTGATATTCCACAAGTTTCATTGGTGATCAATTA
TGATGTACCATTGGATGAAATGGGTAAACCTGACCCAGTTCATTATCTTCATCGTATTGG
TAGAGTTGGTAGATTCGGTCGTTCTGGTGTTGCTCTTAGTTTTGTTTATGATCAACAATC
AACAAATAAATTAATGAATATTTCAACCCATCTTGGTGTACCTTTAAAAGAATTAAAATC
TTCTGAAATTGAATCTTTAGATGGTATCTTAAAAGGTATTAGAAATCAATTAACACCATT
AAAAATTAAAAAAAAACAT
Length of 3' end seq. 669
Connected seq. ID AFH706P
Connected seq. 
>AFH706P.Seq
TTGGCCTACTGGAAATAAATAAATAAATAAAATGTCAGAAAAAGAAACAAATACAACATC
AACAGAAAATAAAGAAAAAGAAAAACAAGAACAAACTAATACAAATTCAACAACAGAATC
AACAAATAATCAAGTTGATGAAGAATATGAAAGACCAGGAAGATCAGAAGGTTTAGATGA
ATTTGGAATTCAATTAGATATTCAACAATCTGATCCAAATTCACCATTATATTCAGTGAA
AACATTTGAAGAATTAGGATTAAAACCAGAGTTATTAAAGGGTGTTTATGCAATGGGTTA
TAATAAACCATCTAAAATTCAAGAAGCAGCATTACCAATTATTATTCAATCACCAAATAA
TTTAATTGCACAATCACAATCAGGTACTGGTAAAACTGCTGCATTCACATTGGGTATGTT
AAATTGTGTTGATCCATCAATCAATGCACCACAAGCAATTT----------TCTGCAACA
TTTAGTATGGGAGTTGAGGAATTGATTAAAAAGATCGTTCAAGATCCATATACAAGTATA
AGATTAAAAAGACAAGAGTTATCTGTTGAAAAGATTCATCAATATTTTATAGATTGTGGT
AGTGAAGATAATAAAGCATTGATTCTATCAGACATCTATGGTTTCATATCAGTTGGTCAG
TCAATTGTATTCGTACATACAATTGCAACTGCAAAATCGGTACACCAAAAAATGGTTGAT
GAAGGTCATTCAGTTTCATTACTCTATGGTAAAGATTTAACAACAGAGGAACGTTTTAAA
CAAATTAAAGATTTCAAAGATGGTAAATCAAAAGTATTGATAACAACAAATGTTTTAGCT
AGAGGTATTGATATTCCACAAGTTTCATTGGTGATCAATTATGATGTACCATTGGATGAA
ATGGGTAAACCTGACCCAGTTCATTATCTTCATCGTATTGGTAGAGTTGGTAGATTCGGT
CGTTCTGGTGTTGCTCTTAGTTTTGTTTATGATCAACAATCAACAAATAAATTAATGAAT
ATTTCAACCCATCTTGGTGTACCTTTAAAAGAATTAAAATCTTCTGAAATTGAATCTTTA
GATGGTATCTTAAAAGGTATTAGAAATCAATTAACACCATTAAAAATTAAAAAAAAACAT
Length of connected seq. 1130
Full length Seq ID -
Full length Seq. -
Length of full length seq. -