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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
FC-AP18
Library FC
(Link to library)
Clone ID FC-AP18
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID FC-AP18Z
(Link to Original site)
Representative DNA sequence 
>FC-AP18 (FC-AP18Q) /CSM/FC/FC-AP/FC-AP18Q.Seq.d/
XXXXXXXXXXTCACATTACCTGTAAATTCACTGAAATCGTCGATAAAGTTAATCGTCGTA
CTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCG
CTATGGNCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCAATGAATACCCAC
CATTAGGTCGTTTCGCCGTCAGAGATATGGGACAAACCGTCGCCGTCGGTGTCATAAAAT
CAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGNTGCCCCATCAA
AAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTTATAATAATTTTAAAAAAA
AAAAAAAAAAAAAAAAA
sequence update 1997. 3.28
Translated Amino Acid sequence 
---HITCKFTEIVDKVNRRTGAVVAKEGTAAVVLKNGDAAMXELTPSRPMCVESFNEYPP
LGRFAVRDMGQTVAVGVIKSTVKKAPGKAGDKKGAXAPSKKK*innis**iafynnfkkk
kkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
---HITCKFTEIVDKVNRRTGAVVAKEGTAAVVLKNGDAAMXELTPSRPMCVESFNEYPP
LGRFAVRDMGQTVAVGVIKSTVKKAPGKAGDKKGAXAPSKKK*innis**iafynnfkkk
kkkkk


Frame B:
---tlpvnslkssiklivvlvpslpkkvlppss*rmvmplwxn*phlvqcvlnpsmnthh
*vvspseiwdkpspsvs*nqpsrkpqvkpvirkvlxphqkrnk*iiflnk*hfiiilkkk
kkkk


Frame C:
---hyl*ih*nrr*s*ssywcrrcqrrycrrrlkew*crygrinpissnvc*ilq*ipti
rsfrrqrygtnrrrrchkinrqespr*sr**ercxcpikkeink*yflinsil**f*kkk
kkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FC-AP18 (FC-AP18Q) /CSM/FC/FC-AP/FC-AP18Q.Seq.d/                      557   e-158
VFM817 (VFM817Q) /CSM/VF/VFM8-A/VFM817Q.Seq.d/                        525   e-148
VFJ401 (VFJ401Q) /CSM/VF/VFJ4-A/VFJ401Q.Seq.d/                        525   e-148
VSI414 (VSI414Q) /CSM/VS/VSI4-A/VSI414Q.Seq.d/                        517   e-146
VSF761 (VSF761Q) /CSM/VS/VSF7-C/VSF761Q.Seq.d/                        517   e-146
VSE380 (VSE380Q) /CSM/VS/VSE3-D/VSE380Q.Seq.d/                        517   e-146
VSD740 (VSD740Q) /CSM/VS/VSD7-B/VSD740Q.Seq.d/                        517   e-146
VSD380 (VSD380Q) /CSM/VS/VSD3-D/VSD380Q.Seq.d/                        517   e-146
VSD312 (VSD312Q) /CSM/VS/VSD3-A/VSD312Q.Seq.d/                        517   e-146
VFO616 (VFO616Q) /CSM/VF/VFO6-A/VFO616Q.Seq.d/                        517   e-146
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   519  e-144  1
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   498  e-137  1
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   321  3e-84  1
AZ932350|AZ932350.1 474.dhz97h09.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz97h09.s1, DNA sequence.    92  1e-17  2
BE449922|BE449922.1 EST400809 tomato root, plants pre-anthesis, Cornell University Lycopersicon esculentum cDNA clone cLEY11I18, mRNA sequence.    90  4e-16  2
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.    94  1e-15  1
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.    94  1e-15  1
Z35987|Z35987.1 S.cerevisiae chromosome II reading frame ORF YBR118w.    92  5e-15  1
M15667|M15667.1 S.cerevisiae EF1 alpha-B gene encoding polypeptide chain elongation factor 1-alpha, complete cds.    92  5e-15  1
M15666|M15666.1 S.cerevisiae EF1 alpha-A gene encoding polypeptide chain elongation factor 1-alpha, complete cds.    92  5e-15  1
dna update 2003. 7.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   147   8e-35
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   147   8e-35
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   147   8e-35
AJ970312_1(AJ970312|pid:none) Hebeloma cylindrosporum mRNA for e...   104   1e-21
FJ695618_1(FJ695618|pid:none) Piriformospora indica translation ...   103   1e-21
FJ539138_1(FJ539138|pid:none) Ignatius tetrasporus strain UTEX B...   102   4e-21
(Q00251) RecName: Full=Elongation factor 1-alpha;          Short...   102   4e-21
AY445082_1(AY445082|pid:none) Metarhizium anisopliae strain E6 t...   100   1e-20
(P40911) RecName: Full=Elongation factor 1-alpha;          Short...   100   1e-20
AY849694_1(AY849694|pid:none) Magnaporthe grisea elongation fact...   100   1e-20
protein update 2009. 4.13
PSORT 

 psg: 0.75  gvh: 0.34  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for FC-AP18 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID FC-AP18Z
3' end seq. 
>FC-AP18Z.Seq
----------TCACATTACCTGTAAATTCACTGAAATCGTCGATAAAGTTAATCGTCGTA
CTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCG
CTATGGNCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCAATGAATACCCAC
CATTAGGTCGTTTCGCCGTCAGAGATATGGGACAAACCGTCGCCGTCGGTGTCATAAAAT
CAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTGATAAGAAAGGTGCTGNTGCCCCATCAA
AAAAGAAATAAATAAATAATATTTCTTAATAAATAGCATTTTATAATAATTTTAAAAAAA
AAAAAAAAAAAAAAAAA
Length of 3' end seq. 367
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -